High Sustained Virologic Response with Daclatasvir plus Asunaprevir in HCV GT-1b Chinese, Korean and Taiwanese without Baseline NS5A Polymorphisms

( F. Mcphee ),( L. Wei ),( Q. Xie ),( Y. Suzuki ),( J. Toyota ),( Y. Karino ),( K. Chayama ),( Y. Kawakami ),( M. L. Yu ),( S. H. Ahn ),( N. Zhou ),( H. Kumada ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Daclatasvir (DCV) plus asunaprevir (ASV) has demonstrated highsustained virologic response (SVR) in HCV genotype (GT-)1b infection.NS5A-Y93H and NS5A-L31 resistance-associated polymorphisms(RAPs) to DCV are known to impact DCV+ASV response in GT-1b-infectedJapanese. The effect of RAPs on SVR at posttreatment week12 (SVR12) to DCV+ASV was explored in mainland Chinese, Korean,and Taiwanese.Methods: Pooled data from 2 studies of DCV (60 mg daily) + ASV(100 mg capsule, twice-daily) for 24 weeks in GT-1b-infected interferon/ribavirin-naive and -experienced patients from mainland China,Korea, and Taiwan. Similar Japanese data (4 studies; n=445) werepooled for comparison. SVR12 with versus without baseline Y93Hand/or L31 RAPs was compared by age (<65 vs ≥65 years), cirrhosisstatus, and baseline HCV-RNA.Results: SVR12 and baseline NS5A sequences were available for 282patients (126 mainland Chinese [45%〕, 80 Koreans [28%〕, 76Taiwanese [27%〕). NS5A-Y93H and/or -L31 RAPs were observed pretreatmentin 8% mainland Chinese, 14% Korean, and 18%Taiwanese patients, compared with 19% in Japanese. SVR12 in allnon-Japanese patients is shown (Figure); rates were broadly similarbetween countries and with Japanese data (Japanese: 96% overallwithout RAPs, 41% with RAPs). Responses were lower among patientswith baseline RAPs. By contrast, SVR12 in patients without RAPs washigh (92-100%), irrespective of cirrhosis, age, or baseline HCV-RNA.Conclusions: At least 95% of HCV GT-1b-infected patients from mainlandChina, Korea or Taiwan without baseline NS5A-Y93H or -L31polymorphisms who had HCV-RNA ≤7 log10 IU/mL achieved SVR12on DCV+ASV, regardless of cirrhosis status and age.

Separation dynamics of hydrogen isotope gas in mesoporous and microporous adsorbent beds at 77 K: SBA-15 and zeolites 5A, Y, 10X

Chu, X.Z.,Cheng, Z.P.,Xiang, X.X.,Xu, J.M.,Zhao, Y.J.,Zhang, W.G.,Lv, J.S.,Zhou, Y.P.,Zhou, L.,Moon, D.K.,Lee, C.H. Pergamon Press ; Elsevier Science Ltd 2014 International journal of hydrogen energy Vol.39 No.9

The separation of a hydrogen isotope mixture on porous materials was studied using equilibrium and breakthrough experiments. The adsorption equilibria of H<SUB>2</SUB> and D<SUB>2</SUB> on SBA-15 with mesopores and molecular sieves 5A, Y, and 10X with micropores were measured at 77 K using the volumetric method. The breakthrough experiments of a H<SUB>2</SUB> and D<SUB>2</SUB> mixture in each adsorbent bed were carried out at various conditions of flow rate and pressure. The equilibrium ratio of D<SUB>2</SUB> to H<SUB>2</SUB> on mesoporous molecular sieves was larger than the ratio on microporous molecular sieves (SBA-15 > 10X > Y > 5A), but the difference among the adsorbents decreased with increases in pressure. On the other hand, the order of breakthrough separation factor showed the opposite result (SBA-15 < 10X < Y < 5A). The breakthrough separation factors for zeolite 10X was approximately equal to the equilibrium ratio of D<SUB>2</SUB> to H<SUB>2</SUB> at the corresponding partial pressures, whereas zeolites 5A and Y showed higher breakthrough separation factors than their equilibrium ratios. In SBA-15, the separation factors from breakthrough results were even smaller than the corresponding equilibrium ratio. In the microporous adsorbent with a limited pore size (zeolite 5A in the study), the diffusion mechanism contributed to the separation of hydrogen isotope gases as one of key factors.

Effects of Fabric Surface Energy on Human Thermophysiological Responses during Exercise and Recovery

Zhou, L.Y.,Li, Y.,Chung, J.,Tokura, H.,Gohel, M.D.I.,Kwok, Y.L.,Feng, X.W. The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.3

The present paper reports a study on influences of fabric surface energy of cotton and polyester garments on clothing microclimates and human thermophysiological responses during intermittent exercise and recovery. Eight healthy males wearing the garments prepared performed exercises and rest according to the following protocol: rest for 30 min, run on treadmill for total 60 min of three sessions with different intensity and duration, and then sit quietly for 30 min for recovery, all at $30^{\circ}C$ and relative humidity of 30 %, while the microclimate humidity ($H_{mc}$) and temperature ($T_{mc}$), the clothing outside surface humidity ($H_{co}$) and temperature ($T_{co}$), the skin temperatures and ear canal temperature ($T_{ear_canal})$ were measured. The garments are made of: (a) hydrophilic and hydrophobic cotton knitted fabrics, and (b) hydrophilic and hydrophobic polyester knitted fabrics. During and after exercise, for cotton, hydrophilic garment resulted in significant lower ${\Delta}H_{mc}$, ${\Delta}H_{co}$, ${\Delta}T_{mc}$, during recovery, higher ${\Delta}{\bar{T}}_{sk}$, lower ${\Delta}T_{ear_canal}$ and ${\Delta}T_{forehead}$. For polyester, hydrophilic garment resulted in significantly lower ${\Delta}H_{co}$, ${\Delta}T_{co}$, higher ${\Delta}T_{forehead}$, during E1, E2 and recovery session but lower during E3. In summary, surface energy of cotton garments had significant influences on human thermophysiological responses during exercise and recovery, and hydrophilic cotton garment was better than hydrophobic one to reduce heat stress. Surface energy of polyester garments had influences of lower significance, and hydrophilic garment appeared better than hydrophobic garment.

Effect of Extenders and Temperatures on Sperm Viability and Fertilizing Capacity of Harbin White Boar Semen during Long-term Liquid Storage

Zhou, J.B.,Yue, K.Z.,Luo, M.J.,Chang, Z.L.,Liang, H.,Wang, Z.Y.,Tan, J.H. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.11

In this study the effect of extenders and temperatures on sperm viability and fertilizing capacity of boar sperm during long-term storage was investigated. Acrosomal integrity, membrane integrity, motility and hypo-osmotic resistance were evaluated by fluorescence and light microscopy. An in vitro fertilization test was performed to assess the fertilizing capacity of stored spermatozoa. The five diluents tested were ranked according to their ability to maintain sperm functional parameters and Zorlesco (ZO) extender with BSA or with PVA instead of BSA produced the best results. Zorlesco extender substituted with PVA (ZO+PVA) was found to maintain motility both at 15 and 20$^{\circ}C$. within 5 days of storage, but the quality of semen stored at 15$^{\circ}C$ decreased thereafter as compared to semen stored at 20$^{\circ}C$ Semen stored at 5$^{\circ}C$ demonstrated rapid loss of motility already within 24 h. Both fertilization and cleavage of semen stored at 20$^{\circ}C$ in ZO substituted with PVA instead of BSA did not change significantly until day 8 of storage. It is therefore concluded that PVA can be used to substitute for BSA and 20$^{\circ}C$ was more suitable than 15$^{\circ}C$ for boar semen storage, and in vitro fertilizing capacity of spermatozoa was maintained for at least 8 days in ZO+PVA at 20$^{\circ}C$.

Study of optical and electrical properties of TiO2/Ag/TiO2 multilayers

J. H. Jia,L. Y. Chen,H. Xie,H. Y. You,J. Li,P. Zhou 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.44 No.32

Optical and electrical properties of TiO2/Ag/TiO2 multilms prepared by a multi-target sputtering method were investigated. The transmittance spectra and the sheet resistance as a function of layer thickness were carefully measured and analyzed. By properly adjusting the thickness of the metal and dielectric lms, a low sheet resistance of 30 Ohms/sq. and a high transmittance of over 90 % in the 500-700 nm wavelength region were achieved. It was found that a Ag layer played a significant role in determining the optical and electrical properties of the film structure.

SOME GENERAL CONVERGENCE PRINCIPLES WITH APPLICATIONS

Zhou, H.Y.,Gao, G.L.,Guo, G.T.,Cho, Y.J. Korean Mathematical Society 2003 대한수학회보 Vol.40 No.3

In the present paper, some general convergence principles are established in metric spaces and then theses principles are applied to the convergence of the iterative sequences for approximating fixed points of certain classes of mappings. By virtue of our principles, most of the latest results obtained by several authors can be deduced easily.

Pharmacokinetics and Metabolism of Streptochlorin and Its Synthetic Derivative, 5-Hydroxy-2′-isobutyl Streptochlorin, in Mice

Zhou, Y.,Choi, Y. J.,Kim, E.,Oh, M. H.,Shin, H. J.,Kim, S. K.,Lee, K. PHARMACEUTICAL SOCIETY OF JAPAN 2018 Biological & pharmaceutical bulletin Vol.41 No.3

<P>The purpose of this study was to investigate the pharmacokinetics and metabolism of streptochlorin and its derivative 5-hydroxy-2'-isobutyl streptochlorin (HIS) in mice. Plasma concentration of streptochlorin declined rapidly resulting in a high sustemic plasma clearance (CLp) (5.8 +/- 1.7L/h/kg), a large volume of distribution (V-ss) (1.4 +/- 0.9L/kg) and a short half-life (t(1/2)) (0.4 +/- 0.1 h) after a single intravenous administration (5mg/kg). Oral bioavailability (F) was 10.3 +/- 3.4% after a single oral administration (10 mg/kg). HIS also showed a rapid plasma decline with a high CLp (11.3 +/- 8.8L/h/kg), a high V-ss (0.8 +/- 1.0L/kg) and a short t(1/2) (0.070 +/- 0.004h) following intravenous administration. It was not detected in plasma after oral administration. Metabolic stability studies using mouse liver microsomes and S9 fractions predicted a high hepatic clearance for both compounds, consistent with the in vivo data. Metabolite identification studies revealed three metabolic pathways for streptochlorin: monooxygenation, glucuronidation of the indole moiety and oxidative opening of the 4-chlorooxazole ring. HIS was metabolized via monooxygenation of the isobutyl chain and glucuronidation of the indole ring. These results may aid in structural optimization to mitigate the metabolic liability of streptochlorin.</P>

Effects of chitooligosaccharide supplementation on performance, blood characteristics, relative organ weight, and meat quality in broiler chickens.

Zhou, T X,Chen, Y J,Yoo, J S,Huang, Y,Lee, J H,Jang, H D,Shin, S O,Kim, H J,Cho, J H,Kim, I H Poultry Science Association, etc 2009 Poultry science Vol.88 No.3

<P>This study was conducted to evaluate the effects of dietary supplementation with chitooligosaccharide (COS) on growth performance, blood characteristics, relative organ weight, and meat quality in broilers. A total of 480 broilers with an average initial BW of 45.04 g per chick were randomly allocated into 1 of the following 4 dietary treatments (20 broilers per pen with 6 pens per treatment): 1) CON (basal diet), 2) ANT (basal diet + 44 mg/kg of avilamycin), 3) COS0.2 (basal diet + 14 g/kg of COS), 4) COS0.4 (basal diet + 28 g/kg of COS). The experiment lasted for 5 wk and avilamycin was administered from d 0 to 21. Growth performance was measured on d 0, 21, and 35, and all other response criteria were measured on d 35. No change in feed conversion (G:F) was observed in response to any of the treatments throughout the experimental period (P > 0.05). However, BW gain and feed intake were greater (P < 0.05) in broilers provided with feed supplemented with COS than in those in the control group. In addition, broilers had significantly greater (P < 0.05) red blood cell and high-density lipoprotein cholesterol concentrations when they were provided with the COS0.4 diet, whereas the triglyceride concentration was lower (P < 0.05) in broilers in the COS0.2 treatment group. No other blood characteristics were affected by the treatments. Additionally, as the dietary COS concentration increased, the liver weight increased (P < 0.05). Conversely, as the concentrations of dietary COS increased, abdominal fat decreased (P < 0.05). Furthermore, meat yellowness decreased (P < 0.05) as the concentration of COS increased. Finally, the breast meat and abdominal fat of birds provided with feed supplemented with COS had a lower (P < 0.05) saturated fatty acid concentration but a greater concentration of total monounsaturated fatty acids (P < 0.05) than that of birds in the control. In conclusion, COS can improve the performance and breast meat quality of broilers while increasing the red blood cell and high-density lipoprotein cholesterol concentrations in blood. In addition, COS can induce a decrease in abdominal fat and improve meat quality.</P>

Effect of Feeding Enzymolytic Soybean Meal on Performance, Digestion and Immunity of Weaned Pigs

Zhou, S.F.,Sun, Z.W.,Ma, L.Z.,Yu, J.Y.,Ma, C.S.,Ru, Y.J. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.1

The objective of this study was to investigate the effect of enzymolytic products of soybean meal (ESBM), as one of the protein sources in the diet, on the growth performance, nutrient digestibility and immune function of weaned piglets. Soybean meal produced by bioprocessing with fermentation and enzymolysis contains reduced anti-nutritional factors and improved protein utilization. A total of 240 weaned piglets (Duroc${\times}$Landrace${\times}$Yorkshire, $9.01{\pm}0.22\;kg$ body weight) were randomly allocated to 4 treatments with 6 pens per treatment and 10 piglets per pen. The diets were based on corn-soybean meal and ESBM partially replaced soybean meal and soybean protein isolate at the inclusion level of 5, 10 or 15% in the basal diet. Feed intake and body weight were measured weekly. On days 24 to 27, faeces of each replicate were proportionally collected to determine the nutrient digestibility. On day 28 of the experiment, one piglet from each replicate was slaughtered humanely to collect immune organs. The results showed that inclusion of ESBM increased (p<0.05) the final weight, daily feed intake and daily gain of weaned pigs compared with the control diet, and ESBM at the inclusion levels of 10 and 15% improved (p<0.05) the feed/gain compared with the control diet. There were no differences (p>0.05) in daily feed intake among the levels of ESBM, but increasing the levels of ESBM from 5 to 15% improved (p<0.05) the final weight, average daily gain of pigs and feed/gain. The inclusion of ESBM at 5 to 15% increased (p<0.05) the digestibility of crude protein (CP) by 5 to 16%, and ESBM at 15% increased (p<0.05) the digestibility of digestible energy (DE), Ca and P compared with the control diet. ESBM increased (p<0.05) the relative weights of thymus and mandibular lymph nodes by 57.7 and 29.6%, respectively. The percentages of T lymphocytes, CD4+ and CD8+ in peripheral blood of weaned piglets were also increased (p<0.05) by feeding ESBM. The results suggest that ESBM can be a better protein source in improving growth performance, nutrient digestibility and immune function of weaned piglets.

Dynamic Threshold Switching Behavior of Ge[sub 2]Sb[sub 2]Te[sub 5] and Sb-doped Ge[sub 2]Sb[sub 2]Te[sub 5] Thin Films Using Scanning Electrical Nanoprobe

Zhou, P.,Shin, Y. C.,Choi, B. J.,Choi, S.,Hwang, C. S.,Lin, Y. Y.,Lv, H. B.,Yan, X. J.,Tang, T. A.,Chen, L. Y.,Chen, B. M. The Electrochemical Society 2007 Electrochemical and solid-state letters Vol.10 No.9