c-Cbl-Mediated Neddylation Antagonizes Ubiquitination and Degradation of the TGF-β Type II Receptor

Zuo, W.,Huang, F.,Chiang, Y.,Li, M.,Du, J.,Ding, Y.,Zhang, T.,Lee, H.,Jeong, L.,Chen, Y.,Deng, H.,Feng, X.H.,Luo, S.,Gao, C.,Chen, Y.G. Cell Press 2013 Molecular cell Vol.49 No.3

Transforming growth factor β (TGF-β) is a potent antiproliferative factor in multiple types of cells. Deregulation of TGF-β signaling is associated with the development of many cancers, including leukemia, though the molecular mechanisms are largely unclear. Here, we show that Casitas B-lineage lymphoma (c-Cbl), a known proto-oncogene encoding an ubiquitin E3 ligase, promotes TGF-β signaling by neddylating and stabilizing the type II receptor (TβRII). Knockout of c-Cbl decreases the TβRII protein level and desensitizes hematopoietic stem or progenitor cells to TGF-β stimulation, while c-Cbl overexpression stabilizes TβRII and sensitizes leukemia cells to TGF-β. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TβRII at Lys556 and Lys567. Neddylation of TβRII promotes its endocytosis to EEA1-positive early endosomes while preventing its endocytosis to caveolin-positive compartments, therefore inhibiting TβRII ubiquitination and degradation. We have also identified a neddylation-activity-defective c-Cbl mutation from leukemia patients, implying a link between aberrant TβRII neddylation and leukemia development.

Structural RC computer aided intelligent analysis and computational performance via experimental investigations

Y.C. Huang,M.D. TuMuli Lulios,Chu-Ho Chang,M. Nasir Noor,Jen-Chung Shao,Chien-Liang Chiu,Tsair-Fwu Lee,Renata Wang 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.90 No.3

This research explores a new finite element model for the free vibration analysis of bi-directional functionally graded (BDFG) beams. The model is based on an efficient higher-order shear deformation beam theory that incorporates a trigonometric warping function for both transverse shear deformation and stress to guarantee traction-free boundary conditions without the necessity of shear correction factors. The proposed two-node beam element has three degrees of freedom per node, and the inter-element continuity is retained using both C1 and C0 continuities for kinematics variables. In addition, the mechanical properties of the (BDFG) beam vary gradually and smoothly in both the in-plane and out-of-plane beam’s directions according to an exponential power-law distribution. The highly elevated performance of the developed model is shown by comparing it to conceptual frameworks and solution procedures. Detailed numerical investigations are also conducted to examine the impact of boundary conditions, the bi-directional gradient indices, and the slenderness ratio on the free vibration response of BDFG beams. The suggested finite element beam model is an excellent potential tool for the design and the mechanical behavior estimation of BDFG structures.

Integrated cladding-pumped multicore few-mode erbium-doped fibre amplifier for space-division-multiplexed communications

Chen, H.,Jin, C.,Huang, B.,Fontaine, N. K.,Ryf, R.,Shang, K.,Gré,goire, N.,Morency, S.,Essiambre, R.-J.,Li, G.,Messaddeq, Y.,LaRochelle, S. Nature Publishing Group 2016 Nature photonics Vol.10 No.8

Space-division multiplexing (SDM), whereby multiple spatial channels in multimode and multicore optical fibres are used to increase the total transmission capacity per fibre, is being investigated to avert a data capacity crunch and reduce the cost per transmitted bit. With the number of channels employed in SDM transmission experiments continuing to rise, there is a requirement for integrated SDM components that are scalable. Here, we demonstrate a cladding-pumped SDM erbium-doped fibre amplifier (EDFA) that consists of six uncoupled multimode erbium-doped cores. Each core supports three spatial modes, which enables the EDFA to amplify a total of 18 spatial channels (six cores × three modes) simultaneously with a single pump diode and a complexity similar to a single-mode EDFA. The amplifier delivers >20 dBm total output power per core and <7 dB noise figure over the C-band. This cladding-pumped EDFA enables combined space-division and wavelength-division multiplexed transmission over multiple multimode fibre spans.

Bone Drilling System for Quantifying Hand Feeling in Orthopedic Surgery

( C. -y. Wu ),( J. -y. Su ),( C. -s. Hsu ),( Y. -h. Chen ),( K. -f. Chang ),( C. -c. Huang ),( H. -m. Wu ),( Y. -c. Tsai ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

In this work, a bone drilling system was designed to quantify “hand feeling”, which can benefit surgeons to be more acquainted with bone hardness. A bi-axial force/torque sensor is mounted on the bone drilling system in order to measure the thrust force and the torque caused by drilling in bones. The system is assembled with a motorized stage, which is directly integrated with a surgical handpiece by an adjustable holder, to drill into various bones (porcine mandible, porcine femur, porcine rib) at different feed speeds and spindle speeds. The results reveal that the feed speed is positively corresponded with force and torque. However, the effect of spindle speed on force and torque is negligible. In addition, drilling bones without spraying water can cause the drilling temperature to increase to 73.3°C. According to the data from the experiments, the exact force and torque during bone drilling can be predicted with the given parameters. Thus, surgeons are capable of preventing the use of excessive force during bone drilling.

The Corrosion Behavior of Cold-Rolled 304 Stainless Steel In Salt Spray Environments

Chiang, M.F.,Young, M.C.,Huang, J.Y. Korean Radioactive Waste Society 2011 방사성폐기물학회지 Vol.9 No.2

염분분위기에서의 부식은 사용후핵연료의 중간저장 기간동안 304 스테인레스 강재 건식저장용기의 주 열화기구들 중 하나다. 본 연구에서는 감소정도가 서로 다른 냉연 304 스테인레스 강 시편들에 0.5wt.%의 염화나트륨 연무를 분사시키면서 느린 변형속도시험(SSRT)과 중성염 분사시험(NSS)을 $85^{\circ}C$와 $200^{\circ}C$ 에서 수행하였다. $85^{\circ}C$에서 2000 시간 동안 시험한 NSS시편의 무게 변화는 $200^{\circ}C$에서 시험한 시편의 무게 변화와 크게 달랐다. NSS 시편의 $85^{\circ}C$에서 무게 감량은 미미하였지만, 냉연 감소율이 증가함에 따라서 무게 변화는 점진적으로 감소하였다. $85^{\circ}C$와 $200^{\circ}C$에서 그리고 염분분사 환경에서 가볍게 냉연 가공된 시편의 SSRT 시험으로부터 얻은 항복강도와 극한 인장응력의 값은 공기 중의 값보다 약간 낮았다. 그러나 염분 분위기에서 부식으로 인한 20% 감소 냉연시편의 강도는 더 이상 변화하지 않았다. 예비결과는 냉연 304 스테인레스 강의 질과 성능이 건식저장용기의 제작을 위한 조건에 맞는다는 것을 증명하였다. 그러나 냉연 스테인레스 강의 장기적인 성능을 더 잘 이해하기 위해서는 염분분위기에서 이 재질의 부식거동에 관한 더 많은 연구가 필요하다. Saline corrosion is one of the major degradation mechanisms for stainless steel type 304 (SS304) dry storage cask during the spent fuel interim storage period. Slow strain rate test (SSRT) and neutral salt spray test (NSS) were performed at $85^{\circ}C$ and $200^{\circ}C$ with 0.5 wt% sodium chloride mist sprayed on the cold-rolled SS304 specimens of different degrees of reduction in this study. The weight changes of the NSS specimens tested at $85^{\circ}C$ for 2000 hours differed greatly from those at $200^{\circ}C$. The weight loss of NSS specimens was not significant at $85^{\circ}C$ but the weight gain decreased gradually with increasing the cold-rolled reduction. The yield strength (YS) and ultimate tensile stress (UTS) values obtained from the SSRT tests for lightly cold-rolled specimens in the salt spray environment at $85^{\circ}C$ and $200^{\circ}C$ are slightly lower than in air. But for those with 20% reductions, the specimen strengths were no longer changed by the saline corrosion. The preliminary results demonstrated that the quality and performance of cold-rolled SS304 is acceptable for fabrication of dry storage casks. However, more work on the corrosion behavior of cold-rolled stainless steel in the saline atmosphere is needed to better understand its long-term performance.

Effects of Wheat Fiber, Oat Fiber, and Inulin on Sensory and Physico-chemical Properties of Chinese-style Sausages

Huang, S.C.,Tsai, Y.F.,Chen, C.M. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.6

This study introduces the addition of wheat fiber, oat fiber, and inulin to Chinese-style sausages, in amounts of 3.5% and 7%, respectively. Researchers used analysis of general composition and texture properties, and sensory evaluation to assess the influence of these three types of dietary fiber on the quality and palatability of Chinese-style sausages. Results showed that the type and amount of dietary fiber introduced did not significantly influence the general composition, color, and total plate count of sausages. However, the addition of wheat fiber and oat fiber significantly hardened the texture of Chinese-style sausages (p<0.05). A greater amount of dietary fiber added implied a harder texture. Added inulin did not influence the texture of Chinese-style sausages (p>0.05). Results of product assessment showed that, aside from sausages with 7% wheat fiber scoring less than 6 points (on a 9-point scale) in terms of overall acceptability, the other groups of Chinese-style sausages scored over 6 points. Judges preferred the sausage groups with 3.5% added oat and wheat fiber. This study demonstrates that adding fiber to Chinese-style sausages to increase the amount of dietary fiber is feasible.

Medicolegal Aspects of Brain Death : The Problem in Intensive Crae Units

Huang, C.Y. Medical Publications Korea 1987 Medical Progress Vol.9 No.9

Analytic Modeling for Current-Voltage Characteristics and Drain-Induced Barrier-Lowering (DIBL) Phenomenon of the InGaP/InGaAs/GaAs PDCFET

C. S Lee,J. C Huang,H. H Chen,W. C Hsu,W. L Yang,Y. J Chen 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.3

A two-dimensional analytic model is proposed for characterizing the current-voltage characteristics and the drain-induced barrier-lowering (DIBL) phenomenon for the InGaP/InGaAs/GaAs pseudomorphic doped-channel eld-eect transistor (PDCFET). The signicant velocity overshoot eects, associated with the low eective mass in the In0:15Ga0:85As channel, have been included to analytically solve the two-dimensional Poisson equation. In addition, by solving the nonlinear equations with the Newton method, this model can be extended to provide a straightforward physical expression of the channel potential prole near or within the sub-threshold regime for short-channel eects. Calculations for the device structure with a gate length of 0.25 m have been conducted, indicating that the pinch-o channel, at a gate-to-source bias of 1.2 V, will resume current conduction as the potential barrier is lowered comparably to the thermal voltage when the drain bias elevates to 2.2 V. This work presents a comprehensive investigation, fast and convenient estimation for the device characteristics and the short-channel eect, and can be extended to multichannel PDCFET structures.

Management of Transient Ischaemic Attacks

Huang, C.Y. Medical Publications Korea 1978 Medical Progress Vol.5 No.11

Probing two-dimensional metallic-like and localization effects at low magnetic fields

Huang, T.Y.,Liang, C.T.,Kim, G.H.,Huang, C.F.,Huang, C.P.,Ritchie, D.A. North-Holland 2010 Physica E, Low-dimensional systems & nanostructure Vol.42 No.4

The metallic-like regime characterized by Shubnikov-de Haas (SdH) oscillations is investigated between localization-induced weak insulator and quantum Hall liquid in a gated two-dimensional GaAs electron system containing InAs dots. Multiple SdH crossing points are observed in the longitudinal resistivity before the appearance of the critical point of a plateau transition with increasing perpendicular magnetic field. In conductivities, however, there is no corresponding crossing in the metallic-like regime because of the T-dependent Hall slope under electron-electron interaction, which provides an explicit way to distinguish SdH crossing points from the critical point in our study.