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      • KCI등재

        Estimation of Water Quality Model Parameters

        김태윤,Y. P. Sheng 대한토목학회 2010 KSCE JOURNAL OF CIVIL ENGINEERING Vol.14 No.3

        A modified Gauss-Newton method was utilized to estimate water quality model parameters, which is important in the modeling of water quality transport process. This method served the unknown parameters of three engineering problems exceptionally well. We applied this method to obtain a set of water quality parameters by solving the kinetic equations for dissolved and particulate water quality species during episodic events in the Indian River Lagoon, FL. The method was successful in producing water quality model parameters, and the relative errors between measurements and model predictions are below 46.4% and 39.4% for all dissolved and particulate water quality state variables, respectively. In this study we evaluated a good capability of suggested parameter estimation method by comparing a couple of water quality model simulations: (1) water quality model simulation using model parameters produced by the modified Gauss-Newton method and (2) water quality model simulation employing model parameters obtained by the trial-and-error method. The average relative error and ROC score between observed data and water quality model results using model parameters calculated by the modified Gauss-Newton method are 58.3% and 0.565, respectively. In the case of water quality model simulation using model parameters obtained by the trial-and-error method, the average relative error and ROC score are 60.5% and 0.555, respectively. This suggests that the modified Gauss-Newton method efficiently yields water quality model parameters resulting in better or similar model prediction errors when compared to the trial-and-error method.

      • Genome-wide Functional Annotation of Dual-Specificity Protein- and Lipid-Binding Modules that Regulate Protein Interactions

        Chen, Y.,Sheng, R.,Kallberg, M.,Silkov, A.,Tun, Moe P.,Bhardwaj, N.,Kurilova, S.,Hall, Randy A.,Honig, B.,Lu, H.,Cho, W. Cell Press 2012 Molecular cell Vol.46 No.2

        Emerging evidence indicates that membrane lipids regulate protein networking by directly interacting with protein-interaction domains (PIDs). As a pilot study to identify and functionally annodate lipid-binding PIDs on a genomic scale, we performed experimental and computational studies of PDZ domains. Characterization of 70 PDZ domains showed that ~40% had submicromolar membrane affinity. Using a computational model built from these data, we predicted the membrane-binding properties of 2,000 PDZ domains from 20 species. The accuracy of the prediction was experimentally validated for 26 PDZ domains. We also subdivided lipid-binding PDZ domains into three classes based on the interplay between membrane- and protein-binding sites. For different classes of PDZ domains, lipid binding regulates their protein interactions by different mechanisms. Functional studies of a PDZ domain protein, rhophilin 2, suggest that all classes of lipid-binding PDZ domains serve as genuine dual-specificity modules regulating protein interactions at the membrane under physiological conditions.

      • KCI등재

        Comparison of tropical cyclone wind field models and their influence on estimated wind hazard

        J.Y. Gu,C. Sheng,H. P. Hong 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.31 No.4

        Engineering type tropical cyclone (TC) wind field models are used to estimate TC wind hazard. Some of the models are well-calibrated using observation data, while others are not extensively compared and verified. They are all proxies to the real TC wind fields. The computational effort for their use differs. In the present study, a comparison of the predicted wind fields is presented by considering three commonly used models: the gradient wind field model, slab-resolving model, and a linear height-resolving model. These models essentially predict the horizontal wind speed at a different height. The gradient wind field model and linear height-resolving model are simple to use while the nonlinear slab-resolving model is more compute-intensive. A set of factors is estimated and recommended such that the estimated TC wind hazard by using these models becomes more consistent. The use of the models, including the developed set of factors, for estimating TC wind hazard over-water and over-land is presented by considering the historical tracks for a few sites. It is shown that the annual maximum TC wind speed can be adequately modelled by the generalized extreme value distribution.

      • SCIESCOPUSKCI등재

        Optimal Design for Marker-assisted Gene Pyramiding in Cross Population

        Xu, L.Y.,Zhao, F.P.,Sheng, X.H.,Ren, H.X.,Zhang, L.,Wei, C.H.,Du, L.X. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

        Marker-assisted gene pyramiding aims to produce individuals with superior economic traits according to the optimal breeding scheme which involves selecting a series of favorite target alleles after cross of base populations and pyramiding them into a single genotype. Inspired by the science of evolutionary computation, we used the metaphor of hill-climbing to model the dynamic behavior of gene pyramiding. In consideration of the traditional cross program of animals along with the features of animal segregating populations, four types of cross programs and two types of selection strategies for gene pyramiding are performed from a practical perspective. Two population cross for pyramiding two genes (denoted II), three population cascading cross for pyramiding three genes(denoted III), four population symmetry (denoted IIII-S) and cascading cross for pyramiding four genes (denoted IIII-C), and various schemes (denoted cross program-A-E) are designed for each cross program given different levels of initial favorite allele frequencies, base population sizes and trait heritabilities. The process of gene pyramiding breeding for various schemes are simulated and compared based on the population hamming distance, average superior genotype frequencies and average phenotypic values. By simulation, the results show that the larger base population size and the higher the initial favorite allele frequency the higher the efficiency of gene pyramiding. Parents cross order is shown to be the most important factor in a cascading cross, but has no significant influence on the symmetric cross. The results also show that genotypic selection strategy is superior to phenotypic selection in accelerating gene pyramiding. Moreover, the method and corresponding software was used to compare different cross schemes and selection strategies.

      • SCIESCOPUSKCI등재

        Genetic Analysis of Haimen Chicken Populations Using Decamer Random Markers

        Olowofeso, O.,Wang, J.Y.,Zhang, P.,Dai, G.J.,Sheng, H.W.,Wu, R.,Wu, X. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.11

        Through a screening and selection approach method, decamer random markers were used in a technique called random amplified polymorphic DNA (RAPD) assay with 252 genomic DNAs isolated from four major Haimen chicken populations: Rugao (62), Jiangchun (62), Wan-Nan (63) and Cshiqishi (65). A total of 3-score decamer random primers (S241-S260, S1081-S1100 and S1341-S1360) were employed in the preliminary RAPD-polymerase chain reaction (RAPD-PCR) assay with 50 random template DNA samples from all the populations. Four (6.67%) of the primers that produced obvious polymorphic patterns, interpretable and reproducible bands were selected and used with both the individual DNAs from each population and with pooled DNA samples of the four populations in subsequent analyses. The selected primers produced a total of 131 fragments with molecular size ranging from 835 to 4,972 base pairs (bp) when used with the individual DNAs; 105 (80.15%) of these fragments were polymorphic. With the pooled DNAs, 47 stable and characteristic bands with molecular size ranging from 840 to 4,983 bp, of which 23 (48.94%) polymorphic, were also generated. The band-sharing coefficient (BSC) calculated for the individuals in the population and among populations of bulked samples was between 0.8247 (Rugao) and 0.9500 (Cshiqishi); for pairwise populations, it was between 0.7273 (Rugao vs. Wan-Nan) and 0.9367 (Jiangchun vs. Cshiqishi) chicken populations. Using the BSC for individual and pairwise populations, the Nei's standard genetic distances between the chicken populations were determined and ranged from 0.0043 (Jiangchun vs. Cshiqishi) to 0.1375 (Rugao vs. Cshiqishi). The reconstructed dendrogram linked the Jiangchun and Cshiqishi chickens as closely related populations, followed by Wan-Nan, while the Rugao was the most genetically distant among the populations.

      • SCIESCOPUSKCI등재

        Estimation of the Cumulative Power of Discrimination in Haimen Chicken Populations with Ten Microsatellite Markers

        Olowofeso, O.,Wang, J.Y.,Shen, J.C.,Chen, K.W.,Sheng, H.W.,Zhang, P.,Wu, R. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.8

        To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ($h_i$) within locus, effective number of alleles ($N_e$) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00${\pm}$0.33 (Rugao) to 4.90${\pm}$0.48 (Cshiqishi) and across populations for all loci was 4.60${\pm}$0.20, while (H) ranged from 0.65${\pm}$0.03 (Rugao) to 0.69${\pm}$0.03 (Jiangchun) among loci and across populations, (H) was 0.67${\pm}$0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.

      • In-situ high-energy synchrotron X-ray diffraction study of micromechanical behavior of multiple phases in Ni<sub>47</sub>Ti<sub>44</sub>Nb<sub>9</sub> shape memory alloy

        Sun, G.A.,Wang, X.L.,Wang, Y.D.,Woo, W.C.,Wang, H.,Liu, X.P.,Chen, B.,Fu, Y.Q.,Sheng, L.S.,Ren, Y. Elsevier Sequoia 2013 Materials science & engineering. properties, micro Vol.560 No.-

        High-energy synchrotron X-ray diffraction technique was used to in-situ characterize microstructure, lattice strain, and phase transition behavior of a Ni<SUB>47</SUB>Ti<SUB>44</SUB>Nb<SUB>9</SUB> shape memory alloy. Phase transformation kinetics and deformation mechanisms were studied under a uniaxial tension at three testing temperatures, i.e., -70<SUP>o</SUP>C, 25<SUP>o</SUP>C, and 150<SUP>o</SUP>C. At a testing temperature of -70<SUP>o</SUP>C, a complicated phase transformation with four stages of micromechanical deformation was identified which is associated with changes of martensite substructures. At room temperature of 25<SUP>o</SUP>C, there was no stress-induced selection process of variants of B19' phases observed. Whereas at a testing temperature of 150<SUP>o</SUP>C, there was no any phase transformation observed. It is verified that β-Nb phase, an effective stabilizer for the austenite, delays the process of martensitic transformation and relaxes the strain energy without strengthening the matrix. This new finding is important to understand the relationship between the micromechanical deformation behavior and phase transformations in the Ni<SUB>47</SUB>Ti<SUB>44</SUB>Nb<SUB>9</SUB> SMA.

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