Prepartum body condition score affects milk yield, lipid metabolism, and oxidation status of Holstein cows

Wei Zhao,Xue Chen,Jun Xiao,Xiao Hui Chen,Xue Feng Zhang,Tao Wang,Yu Guo Zhen,Gui Zin Qin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.12

Objective: This study aimed to investigate the effects of prepartum body condition score (BCS) on the milk yield, lipid metabolism, and oxidative status of Holstein cows. Methods: A total of 112 multiparous Holstein cows were divided into 4 groups according to the BCS at 21 days before calving: medium BCS (3.0 to 3.25, MBCS), high BCS (3.5 to 3.75, HBCS), higher BCS (4.0 to 4.25, HerBCS), and highest BCS (4.5 to 5.0, HestBCS). Blood samples were collected on 21, 14, and 7 days before calving (precalving), on the calving day (calving), and on 7, 14, and 21 days after calving (postcalving). The indices of lipid metabolism and oxidative status were analyzed using bovine-specific enzyme-linked immunosorbent assay kit. Colostrum were taken after calving and analyzed by a refractometer and milk analyzer. The individual milk yield was recorded every 3 days. Results: The density and levels of immune globulin and lactoprotein of colostrum from Holstein cows in the HestBCS group were the highest (p<0.05). These animals not only had the highest (p<0.05) levels of serum non-esterified fatty acids and beta-hydroxybutyrate, but also had the highest (p<0.05) levels of malondialdehyde, superoxide dismutase, catalase, vitamin A, and vitamin E. In addition, greater (p<0.05) BCS loss was observed in the HestBCS cows. Conclusion: This study demonstrates that the milk yield, lipid metabolism, and oxidative status of Holstein cows are related to prepartum BCS and BCS loss during the transition period. HestBCS cows are more sensitive to oxidative stress and suffer greater loss of BCS after calving, whereas the MBCS animals had better milk yield performance.

Chemical Constituents from the Aerial Parts of Isodon coetsa and their Cytotoxicity

Wei Zhao,Jian Xin Pu,Xue Du,Yong Zhao,Fei He,Hai Bo Zhang,Yong Bo Xue,Wei Lie Xiao,Han Dong Sun,Ying Li Wu,Guo Qiang Chen 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12

Three new compounds (1-3), including a neolignan, a triterpenoid, and a diterpenoid, together with twenty known compounds (4-23), were isolated from the aerial parts of Isodon coetsa. Their structures and relative configurations were elucidated on the basis of spectroscopic data. Compounds 1, 3, 5-9, 11-13, 16-17, and 19-23 were evaluated for their cytotoxicity against HT-29, BEL-7402, and SK-OV-3 human tumor cell lines. Compound 7 showed significant inhibitory effects on all three types of cells, with IC50 values of 2.52, 3.06, 2.14 μM, respectively.

An Improved Integrative Transformation System for Pichia pastoris with DNA-polyethylenimine-dextran Sulfate Nanoparticles

Wei Xue,Hang Wang,Zuodi Pei,Wei Zhao,Yong-Sheng Tian,Ri-He Peng,Quan-Hong Yao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3

Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it difficult to obtain a large number of transformants necessary to thoroughly cover a large library. We report here that pre-coating DNA with polyethyleneimine and dextran sulfate increased gene integration significantly in Pichia electroporation. This improvement on integrative efficiency has been proved in different voltage, resistance, cell phase, and DNA concentrations. The condensed DNA nanoparticles make Pichia available as a host for screening large libraries of random mutants.

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Du, Wei,Ma, Xue-Lei,Zhao, Chong,Liu, Tao,Du, Yu-Liang,Kong, Wei-Qi,Wei, Ben-Ling,Yu, Jia-Yun,Li, Yan-Yan,Huang, Jing-Wen,Li, Zi-Kang,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Background: MicroRNAs (miRNAs) are an abundant class of endogenous small non-coding RNAs of 20-25 nucleotides in length that function as negative gene regulators. MiRNAs play roles in most biological processes, as well as diverse human diseases including cancer. Recently, many studies investigated the association between SNPs in miR-146a rs2910164, miR-196a2 rs11614913, miR-149 rs229283, miR-499 rs3746444 and colorectal cancer (CRC), which results have been inconclusive. Methodology/Principal Findings: PubMed, EMBASE, CNKI databases were searched with the last search updated on November 5, 2013. For miR-196a2 rs11614913, a significantly decreased risk of CRC development was observed under three genetic models (dominant model: OR = 0.848, 95%CI: 0.735-0.979, P = 0.025; recessive model: OR = 0.838, 95%CI: 0.721-0.974, P = 0.021; homozygous model: OR = 0.754, 95%CI: 0.627-0.907, P = 0.003). In the subgroup analyses, miR-$196a2^*T$ variant was associated with a significantly decreased susceptibility of CRC (allele model: OR = 0.839, 95%CI: 0.749-0.940, P = 0.000; dominant model: OR = 0.770, 95%CI: 0.653-0.980, P = 0.002; recessive model: OR = 0.802, 95%CI: 0.685-0.939, P = 0.006; homozygous model: OR = 0.695, 95%CI: 0.570-0.847, P = 0.000). As for miR-149 rs2292832, the two genetic models (recessive model: OR = 1.199, 95% CI 1.028-1.398, P = 0.021; heterozygous model: OR = 1.226, 95% CI 1.039-1.447, P = 0.013) demonstrated increased susceptibility to CRC. On subgroup analysis, significantly increased susceptibility of CRC was found in the genetic models (recessive model: OR = 1.180, 95% CI 1.008-1.382, P = 0.040; heterozygous model: OR = 1.202, 95% CI 1.013-1.425, P = 0.013) in the Asian group. Conclusions: These findings supported that the miR-196a2 rs11614913 and miR-149 rs2292832 polymorphisms may contribute to susceptibility to CRC.

Antiproliferative Effects of Celecoxib in Hep-2 Cells through Telomerase Inhibition and Induction of Apoptosis

Zhao, Yong-Qiang,Feng, Hui-Wei,Jia, Tao,Chen, Xue-Mei,Zhang, Hui,Xu, An-Ting,Zhang, Hai-Ling,Fan, Xian-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: To investigate the effect of celecoxib on telomerase activity and apoptosis in a human laryngeal squamous carcinoma cell line (Hep-2 cells). Materials and Methods: The growth inhibition rate of Hep-2 cells in vitro was measured by MTT assay, and apoptosis by TUNEL assay and flow cytometry (FCM). The TRAP-ELISA method was used to determine telomerase activity in Hep-2 cells. The mRNA expression of human telomerase RNA component(hTR), human telomerase reverse transcriptase (hTERT) and human telomerase-associated protein(hTEP1) was determined by RT-PCR assay. Expression of Bax and Bcl-2 proteins was assessed by Western blotting. Results: Celecoxib can inhibit proliferation and induce apoptosis in a dose- and time-dependent manner, repress telomerase activity, decrease hTERT mRNA and Bcl-2 protein expression and increase Bax protein expression, PGE2 had no effect on telomerase. Conclusions: Celecoxib had the antiproliferative and pro-apoptotic effect in Hep-2 cells. Apoptosis was accompanied by a decrease in telomerase activity which was directly correlated with hTERT mRNA and up-regulation of Bax/Bcl-2. Bcl-2 may thus play an important role in telomerase activity as well as apoptosis.

자우주제 : 노가재(老稼齋)의 예술소양(藝術素養)과 연행(燕行) 문화교류(文化交流)

( Xue Tang Li ),( Hua Wei Zhao ),( Hong Wei ) 동방한문학회 2014 東方漢文學 Vol.0 No.58

조선후기 문인으로서 노가재는 연행을 통해 청나라의 정치사회와 경제발전 상황, 문화풍속 등을 두루 견문하였다. 그는 조선 선비로서의 긍지를 가지고 연행에 나섰으며 문명인의 모습을 보여주면서 자각적으로 문화 교류의 사절 역할을 담당하였다. 이번 연행의 성과물로써 삼대 연행록의 白眉로 평가받는 『老稼齋燕行日記』를 전대의 여타 연행록들과 구별짓는 중요한 특색은 노가재가 예리한 안목과 뛰어난 필치로 청나라의 사회상을 그려낸 것이다. 본 논문은 노가재의 남다른 예술적 자질을 특히 주목하고, 그가 연행 과정에서 견문한 연예, 환술 등 청나라 민간 문화예술에 대한 기술을 종합 분석하여 이 연행일기의 한중문화교류사상의 의미를 새로이 부각할 것을 목적으로 한다. 이렇기 위하여 먼저 노가재의 예술적 지향과 재능을 고찰한 다음 예술 부문을 나누어 연행과정에서의 견문과 기록 내용을 구체적으로 분석하였다. 그 결과, 노가재의 남다른 예술적 소양이 그의 기행문의 예술적 분위기를 특히 농후하게 하였다는 것을 확인할 수 있었다. 그는 연행일기에서 적지 않은 편폭으로 청나라 민간 예술인들의 연예와 환술, 공예품 등 문화예술과 관련한 기술을 남겼다. 이러한 내용들은 청나라의 문화사를 규명하는데 중요한 역사 자료로 활용할 수 있을 뿐만 아니라, 조선지식인의 청나라 문화에 대한 인식 태도를 알아보는데도 중요한 의미를 갖는다. From a young age, Kajae began to try some art activities such as literary creation and music art, which gave him a unique artistic accomplishment and enthusiasm of becoming a practice-based rural artist. All of those rich experiences became one of the preconditions and sources of the Diary of Kajae`s Traveling in Peking, his immortal classics. According to the related contents in this paper, we can found that because of his artistic accomplishment, there is an obvious feature in his travel note, which with dense academic atmosphere of art. He would describe what he heard or saw faithfully by chronological narrative according to the chronological and ordinal order. His skill of recording well-known mountains and rivers is symbolic almost to the point of landscape painting. Meanwhile, just because he can appreciate instruments, music, painting, handiwork, folk drama, vaudeville and magic, he can observe and record basic features and value of these folk arts that can`t be found by ordinary people. Diary of Kajae`s Traveling in Peking is a comprehensive diary style literary work with rich content and its own logic and system, as well as description of scenery and objects. That is the unique feature of this work, which can manifest its value both in history and literature.

Evaluation of Biogas Production Performance and Dynamics of the Microbial Community in Different Straws

( Xue Li ),( Yan-hua Liu ),( Xin Zhang ),( Chang-ming Ge ),( Ren-zhe Piao ),( Wei-dong Wang ),( Zong-jun Cui ),( Hong-yan Zhao ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The development and utilization of crop straw biogas resources can effectively alleviate the shortage of energy, environmental pollution, and other issues. This study performed a continuous batch test at 35°C to assess the methane production potential and volatile organic acid contents using the modified Gompertz equation. Illumina MiSeq platform sequencing, which is a sequencing method based on sequencing-by-synthesis, was used to compare the archaeal community diversity, and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community diversity in rice straw, dry maize straw, silage maize straw, and tobacco straw. The results showed that cumulative gas production values for silage maize straw, rice straw, dry maize straw, and tobacco straw were 4,870, 4,032.5, 3,907.5, and 3,628.3 ml/g ·VS , respectively, after 24 days. Maximum daily gas production values of silage maize straw and rice straw were 1,025 and 904.17 ml/g ·VS, respectively, followed by tobacco straw and dry maize straw. The methane content of all four kinds of straws was > 60%, particularly that of silage maize straw, which peaked at 67.3%. Biogas production from the four kinds of straw was in the order silage maize straw > rice straw > dry maize straw > tobacco straw, and the values were 1,166.7, 1,048.4, 890, and 637.4 ml/g ·VS, respectively. The microbial community analysis showed that metabolism was mainly carried out by acetateutilizing methanogens, and that Methanosarcina was the dominant archaeal genus in the four kinds of straw, and the DGGE bands belonged to the phyla Firmicutes, Bacteroidetes, and Chloroflexi. Silage maize is useful for biogas production because it contains four kinds of straw.

Arabidopsis thaliana Metallothionein, AtMT2a, Mediates ROS Balance during Oxidative Stress

Wei Zhu,Dong-Xiao Zhao,Qing Miao,Tong-Tong Xue,Xin-Zheng Li,Cheng-Chao Zheng 한국식물학회 2009 Journal of Plant Biology Vol.52 No.6

Cold stress has been shown to induce the production of reactive oxygen species (ROS), which can elicit a potentially damaging oxidative burden on cellular metabolism. Here, the expression of a metallothionein gene (AtMT2a) was upregulated under low temperature and hydrogen peroxide (H2O2) stresses. The Arabidopsis T-DNA insertion mutant, mt2a, exhibited more sensitivity to cold stress compared to WT plants during the seed germination, and H2O2 levels in mt2a mutant were higher than that in WT plants during the cold stress. Synthetic GFP fused to AtMT2a was observed to be localized in cytosol. These results indicated that AtMT2a functions in tolerance against cold stress by mediating the ROS balance in the cytosol. Interestingly, mRNA level of AtMT2a was increased in seedlings of Arabidopsis cat2 mutant after cold treatment compared to WT seedlings, and overexpression of AtMT2a in cat2 could improve CAT activity under chilling stress. Moreover, the enzymatic activity of CAT in mt2a was higher than that in WT plants after cold treatment, suggesting that AtMT2a and CAT might complement each other in antioxidative process potentially in Arabidopsis. Taken together, our results provided a novel insight into the relationship between MTs and antioxidative enzymes in the ROS-scavenging system in plants.

ATAD2 is Highly Expressed in Ovarian Carcinomas and Indicates Poor Prognosis

Wan, Wei-Na,Zhang, Yi-Xia,Wang, Xue-Mei,Liu, Yan-Jun,Zhang, Yu-Qin,Que, Yan-Hong,Zhao, Wen-Jing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

The purpose of this study was to explore the expression of ATAD2 in ovarian tumor tissue as well as its relationship with degree of malignancy. Tumor tissue from 110 cases of ovarian cancer was collected in accordance with the Declaration of Helsinki for evaluation of ATAD2 expression iimmunohistochemistry, quantitative PCR (qPCR) and Western blotting. The correlation between the ATAD2 expression and and the prognosis of ovarian cancer was evaluated by Cox regression model. In addition, HO-8910 and OVCAR-3 cells were transfected with two siRNAs targeting ATAD2. Cell viability was evaluated with MTT assay, and cell migration by transwell migration assay. ATAD2 was shown to be highly expressed in 65.5% (72/110) of ovarian cancer cases, both at transcriptional and protein levels. Moreover, highly expression was positively correlated with degree of malignancy. Knock-down of ATAD2 in HO-8910 and OVCAR-3 cells was found to reduce cell migration. In addition, follow-up visits of the patients demonstrated that the 5-year survival rate was lower in patients with high expression of ATAD2. Our study suggested that ovarian tumor tissue may have highly expressed ATAD2, which is associated with tumor stage, omentum-metastasis, ascites and CA-125. Increased ATAD2 may play important roles in tumor proliferation and migration. ATAD2 could serve in particular as a prognostic marker and a therapeutic target for ovarian cancer.

Attitude Optimization Control Method of Agile Optical Small Satellite for Nonparallel Ground Track Imaging

Guo-Wei Fan,Xue-Ying Lv,Ying Song,Huan-Yu Zhao,Liu Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.8

Nonparallel ground track imaging of optical remote sensing satellite is an efficient imaging mode which could realize one-time transit imaging of complex observation tasks due to its fast and flexible imaging capability, and has attracted more attentions in the earth observation field recently. In this paper, an off-line attitude trajectory planning and on-line moving horizon attitude tracking control scheme based on optimal control idea is designed to satisfy the imaging requirement of high-performance attitude control oriented to nonparallel ground track imaging. Considering the entire satellite attitude adjustment of the drift angle, based on the geometric relationship of sapce vectors and the coordinate transformation principle, the three-axis attitude calculation of the precise pointing of key feature targets in the interested ground curve strip is conducted. Taking the attitude orientation as the constraint, the pseudospectral method is used following the established agile satellite control model to design the optimal trajectory of the attitude manever and the feedforward control torque of actuator for nonparallel ground track imaging. A moving horizon tracking control law based on the nonlinear error control model is designed to realize the highprecision attitude tracking control. The feasibility and effectiveness of the proposed control scheme are illustrated by numerical and imaging simulations.