Minor constituents from the Roots of Sophora flavescens

Ju Sun Kim,Sang Jun Han,Ji Hye Byun,Yong Nan Xu,Sang Woo Yoo,Sam Sik Kang,Kun Ho Son,Hyeun Wook Chang,Hyun Pyo Kim 한국생약학회 2001 Natural Product Sciences Vol.7 No.1

Lupenone, hexadecyl ferulate, (-)-sophocarpine and three isoflavonoids such as genistein, 3`-methoxydaidzein and calycosin were isolated from the roots of Sophora flavescens.

Synthesis and Characterization of Magnetic Nanoparticles and Its Application in Lipase Immobilization

Xu, Jiakun,Ju, Caixia,Sheng, Jun,Wang, Fang,Zhang, Quan,Sun, Guolong,Sun, Mi Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

We demonstrate herein the synthesis and modification of magnetic nanoparticles and its use in the immobilization of the lipase. Magnetic $Fe_3O_4$ nanoparticles (MNPs) were prepared by simple co-precipitation method in aqueous medium and then subsequently modified with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were synthesized successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its derivatives were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) analysis, X-ray diffraction, superconducting quantum interference device (SQUID) and thermogravimetric analyses (TGA). All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47.9 emu/g, 33.0 emu/g and 19.5 emu/g, respectively. The amino content was 5.66%. The AMNP was used to immobilize lipase, and the maximum adsorption capacity of the protein was 26.3 mg/g. The maximum maintained activity (88 percent) was achieved while the amount of immobilized lipase was 23.7 mg $g^{-1}$. Immobilization of enzyme on the magnetic nanoparticles can facilitate the isolation of reaction products from reaction mixture and thus lowers the cost of enzyme application.

Drug-loaded microbubble delivery system to enhance PD-L1 blockade immunotherapy with remodeling immune microenvironment

Zheng Jun,Huang Ju,Zhang Liang,Wang Mengna,Xu Lihong,Dou Xiaoyun,Leng Xiaojing,Fang Mingxiao,Sun Yang,Wang Zhigang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Although programmed cell death protein 1 (PD-1)/ programmed cell death-ligand protein 1 (PD-L1) checkpoint blockade immunotherapy demonstrates great promise in cancer treatment, poor infiltration of T cells resulted from tumor immunosuppressive microenvironment (TIME) and insufficient accumulation of anti-PD-L1 (αPD-L1) in tumor sites diminish the immune response. Herein, we reported a drug-loaded microbubble delivery system to overcome these obstacles and enhance PD-L1 blockade immunotherapy.Docetaxel (DTX) and imiquimod (R837)-loaded microbubbles (RD@MBs) were synthesized via a typical rotary evaporation method combined with mechanical oscillation. The targeted release of drugs was achieved by using the directional "bursting" capability of ultrasound-targeted microbubble destruction (UTMD) technology. The antitumor immune response by RD@MBs combining αPD-L1 were evaluated on 4T1 and CT26 tumor models.The dying tumor cells induced by DTX release tumor-associated antigens (TAAs), together with R837, promoted the activation, proliferation and recruitment of T cells. Besides, UTMD technology and DTX enhanced the accumulation of αPD-L1 in tumor sites. Moreover, RD@MBs remolded TIME, including the polarization of M2-phenotype tumor-associated macrophages (TAMs) to M1-phenotype, and reduction of myeloid-derived suppressor cells (MDSCs). The RD@MBs + αPD-L1 synergistic therapy not only effectively inhibited the growth of primary tumors, but also significantly inhibited the mimic distant tumors as well as lung metastases.PD-L1 blockade immunotherapy was enhanced by RD@MBs delivery system.

Synthesis and Characterization of Magnetic Nanoparticles and Its Application in Lipase Immobilization

Jiakun Xu,Caixia Ju,Jun Sheng,Fang Wang,Quan Zhang,Guolong Sun,Mi Sun 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8

We demonstrate herein the synthesis and modification of magnetic nanoparticles and its use in the immobilization of the lipase. Magnetic Fe3O4 nanoparticles (MNPs) were prepared by simple co-precipitation method in aqueous medium and then subsequently modified with tetraethyl orthosilicate (TEOS) and 3- aminopropyl triethylenesilane (APTES). Silanization magnetic nanoparticles (SMNP) and amino magnetic nanomicrosphere (AMNP) were synthesized successfully. The morphology, structure, magnetic property and chemical composition of the synthetic MNP and its derivatives were characterized using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) analysis, X-ray diffraction, superconducting quantum interference device (SQUID) and thermogravimetric analyses (TGA). All of these three nanoparticles exhibited good crystallization performance, apparent superparamagnetism, and the saturation magnetization of MNP, SMNP, AMNP were 47.9 emu/g, 33.0 emu/g and 19.5 emu/g, respectively. The amino content was 5.66%. The AMNP was used to immobilize lipase, and the maximum adsorption capacity of the protein was 26.3 mg/g. The maximum maintained activity (88 percent) was achieved while the amount of immobilized lipase was 23.7 mg g−1. Immobilization of enzyme on the magnetic nanoparticles can facilitate the isolation of reaction products from reaction mixture and thus lowers the cost of enzyme application.

Correlation Between Mammograghic Findings and Clinical/Pathologic Features in Women with Small Invasive Breast Carcinomas

Li, Jun-Nan,Xu, Jing,Wang, Ju,Qing, Chun,Zhao, Yu-Mei,Liu, Pei-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: To study the relationship between mammographic findings and clinical/pathologic features in women with 1-15mm sized invasive breast cancer. Materials and Methods: We investigated a consecutive series of 134 cases diagnosed in Tianjin Medical University Cancer Institute and Hospital in 2007. Mammographic findings were classified into five groups as follows :1) stellate mass without calcification; 2) non-stellate mass without calcification; 3) intermediate suspicious calcification with or without associated mass; 4) higher probability malignant calcification with or without associated mass; 5) focal asymmetry/distortion without associated calcification. Associations between mammographic and clinical/pathological features (menopause status/family history/histologic grade/lymph node status and ER/PR/HER2 status) was analyzed through logistic regression and chi square tests. Results: Compared to the stellate mass without calcification group, higher probability malignant calcification patients were associated significantly with a positive lymph node status, always presenting in patients who were non-menopausal and with a family history of carcinoma. Conclusions: Higher probability malignant calcifications with or without associated tumor masses are associated with clinical/pathologic features of poor prognosis.

Minor Constituents from the Roots of Sophora flavescens

Kim, Ju Sun,Han, Sang Jun,Byun, Ji Hye,Xu, Yong Nan,Yoo, Sang Woo,Kang, Sam Sik,Son, Kun Ho,Chang, Hyeun Wook,Kim, Hyun Pyo 영남대학교 약품개발연구소 2001 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

Lupenone, hexadecyl fenulate, (-)-sophocarpine and threeisoflavonoids such as genistein, 3'-methoxydaidzein and calycosin were isolated from the roots of Sophora flavescens.

Stability analysis of continuous-time systems with time-varying delay using new Lyapunov–Krasovskii functionals

Chen, Jun,Park, Ju H.,Xu, Shengyuan Elsevier 2018 Journal of the Franklin Institute Vol.355 No.13

<P><B>Abstract</B></P> <P>This paper studies the stability of linear continuous-time systems with time-varying delay by employing new Lyapunov–Krasovskii functionals. Based on the new Lyapunov–Krasovskii functionals, more relaxed stability criteria are obtained. Firstly, in order to coordinate with the use of the third-order Bessel-Legendre inequality, a proper quadratic functional is constructed. Secondly, two couples of integral terms { ∫ t − <SUB> h t </SUB> s x ( s ) d s , ∫ s t x ( s ) d s } and { ∫ t − <SUB> h M </SUB> s x ( s ) d s , ∫ s t − <SUB> h t </SUB> x ( s ) d s } are involved in the integral functionals ∫ t − <SUB> h t </SUB> t ( · ) d s and ∫ t − <SUB> h M </SUB> t − <SUB> h t </SUB> ( · ) d s , respectively, so that the coupling information between them can be fully utilized. Finally, two commonly-used numerical examples are given to demonstrate the effectiveness of the proposed method.</P>

Water-Soluble MnO Nanocolloid for a Molecular <i>T</i>₁ MR Imaging: A Facile One-Pot Synthesis, In vivo <i>T</i>₁ MR Images, and Account for Relaxivities

Baek, Myung Ju,Park, Ja Young,Xu, Wenlong,Kattel, Krishna,Kim, Han Gyeol,Lee, Eun Jung,Patel, Anilkumar Kantilal,Lee, Jae Jun,Chang, Yongmin,Kim, Tae Jeong,Bae, Ji Eun,Chae, Kwon Seok,Lee, Gang Ho American Chemical Society 2010 ACS APPLIED MATERIALS & INTERFACES Vol.2 No.10

<P>A facile one-pot synthesis of a water-soluble MnO nanocolloid (i.e., <SMALL>d</SMALL>-glucuronic acid-coated MnO nanoparticle) is presented. The MnO nanoparticle in the MnO nanocolloid was coated with a biocompatible and hydrophilic <SMALL>d</SMALL>-glucuronic acid, and its particle diameter was nearly monodisperse and ranged from 2 to 3 nm. The average hydrodynamic diameter of the MnO nanocolloid was estimated to be 5 nm. The MnO nanoparticle was nearly paramagnetic down to <I>T</I> = 3 K. The MnO nanocolloid showed a high longitudinal water proton relaxivity of <I>r</I><SUB>1</SUB> = 7.02 s<SUP>−1</SUP> mM<SUP>−1</SUP> with the <I>r</I><SUB>2</SUB>/<I>r</I><SUB>1</SUB> ratio of 6.83 due to five unpaired <I>S</I>-state electrons of Mn(II) ion (<I>S</I> = 5/2) as well as a high surface to volume ratio of the MnO nanoparticle. High contrast in vivo <I>T</I><SUB>1</SUB> MR images were obtained for various organs, showing the capability of the MnO nanocolloid as a sensitive <I>T</I><SUB>1</SUB> MRI contrast agent. The suggested three key-parameters which control the <I>r</I><SUB>1</SUB> and <I>r</I><SUB>2</SUB> relaxivities of nanocolloids (i.e., the <I>S</I> value of a metal ion, the spin structure, and the surface to volume ratio of a nanoparticle) successfully accounted for the observed <I>r</I><SUB>1</SUB> and <I>r</I><SUB>2</SUB> relaxivities of the MnO nanocolloid.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2010/aamick.2010.2.issue-10/am100641z/production/images/medium/am-2010-00641z_0012.gif'></P>

Risk Factors, Patterns, and Outcomes of Late Recurrence after Liver Resection for Patients with Hepatocellular Carcinoma (Analysis of a Multicenter Cohort over 15 Years)

( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Hao Xing ),( Jun Han ),( Zhen-li Li ),( Han Wu ),( Han Zhang ),( Jian-hong Zhong ),( Yi- Sheng Huang ),( Ya-hao Zhou ),( Ting-hao Chen ),( Hong Wang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Late recurrence (> 2 years) after liver resection of hepatocellular carcinoma (HCC) is usually considered as multi-centric tumors or de novo cancer formation. We aimed to investigate risk factors, patterns and outcomes of late recurrence after HCC resection. Methods: From a multicenter database from 2001 to 2015, 734 patients who were alive and recurrence-free at 2 years after curative resection of initial HCC were enrolled into this retrospective study. Univariate and multivariate Cox-regression analysis were used to identify independent risk factors of late recurrence. Patterns, treatments and outcomes of late recurrence were investigated and analyzed. Results: During a median follow-up of 78.0 months after surgery, 303 patients (41.3%) developed late recurrence. Multivariate analysis revealed that cirrhosis, macroscopic vascular invasion, satellites, and tumor size > 5cm were independent risk factors of late recurrence. Among them, 273 (90.1%) were sole intrahepatic recurrence, 30 (9.9%) were concurrent intrahepatic and extrahepatic recurrence, and none of them was sole extrahepatic recurrence; 165 (54.4%) patients received curative treatments for recurrent HCC, including re-resection, transplantation and local ablation. Multivariate analysis showed regular postoperative surveillance and receiving curative treatments were two independent protective factors of prolonging survival for those patients with late recurrence. Conclusions: Late recurrence is correlated with cirrhosis and certain tumor-related characteristics of initial HCC. The patterns of late recurrence suggest that postoperative surveillance after 2 years of surgery could be adjusted and more targeted. Regular postoperative surveillance improves the probability to receive curative treatments again, yielding to better outcomes for patients with late recurrence.

Is Surgical Resection Justified for Hepatocellular Carcinoma with Portal Vein Tumor Thrombus? (A Systematic Review and Meta-Analysis)

( Liang Lei ),( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Zhen-li Li ),( Jun Han ),( Han Zhang ),( Hao Xing ),( Han Wu ),( Ming-da Wang ),( Chao Li ),( Zheng Wang ),( Feng Shen ),( Meng-chao Wu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The prognosis of hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is very poor. According to the BCLC treatment recommendations, sorafenib or other palliative treatment (PT) is recommended as the first-line therapy when it happens. In real world, however, a significant number of selected patients with HCC and PVTT suffered from surgical resection (SR). Methods: PubMed, Embase, Medline and Cochrane library were searched for studies comparing SR with PT (including TACE, sorafenib, etc.) for HCC with PVTT, which were published before September 2017. Results: 4,810 patients from 7 studies were enrolled in this meta-analysis, which divided into the SR group (n = 2,344) and the PT group (n = 2476). When compared with the PT group, the pooled hazard ratio (HR) for the 1, 3 and 5-year OS rates of the SR group were 0.56 (95% CI 0.52-0.60, P=0.03), 0.56 (95% CI 0.53-0.59, P<0.001) and 0.55 (95% CI 0.54-0.57, P<0.001). For subgroup analysis, when compared with the mere TACE group, the pooled HR for the 1, 3 and 5-year OS rates of the SR group were 0.54 (95% CI 0.43-0.67, P=0.81), 0.75 (95% CI 0.65-0.87, P=0.25) and 0.76 (95% CI 0.67-0.88, P=0.25). Conclusions: This meta-analysis demonstrated SR had better OS than TACE or other palliative therapy for HCC with PVTT. SR may be suitable as the first-line treatment for selected patients with resectable HCC and removable PVTT.