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RISS 인기검색어
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- 저자 : Xing Binglin (검색결과 2 건)
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Green and mild production of 5-aminolevulinic acid from algal biomass
Binglin Chen,Jiachen Li,Yunchao Feng,Kai Le,Yuxia Zai,Xing Tang,Yong Sun,Xianhai Zeng,Lu Lin 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5
Algal biomass was converted into 5-aminolevulinic acid (5-ALA) in five chemical steps: conversion to 5- (chloromethyl)furfural (5-CMF), ammoniation, ring-opening (photo-oxidation), reduction, and hydrolyzation. Among them, we mainly focused on the 5-CMF production and the following ammoniation. To our knowledge, the mixed solvent catalytic system of deep eutectic solvent (DES) and low concentration hydrochloric acid is the first reported for the synthesis of 5-CMF from algal biomass, providing a 24.6% 5-CMF yield at 120 oC for 5 h. Potassium phthalimide (KPI) was employed as an ammoniation reagent with superb selectivity and activity instead of conventional sodium azide (NaN3). Optimizing the experimental design, a 23.7% 5-ALA yield along with high purity (>96%) was achieved from 5-CMF, and the total 5-ALA yield was 5.8% from algal biomass. This work provides a green and mild pathway for 5- ALA production from algal biomass.
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Yang Lei,Xing Binglin,Wang Likui,Yuan Linlin,Manzoor Mujahid,Li Fen,Wu Shaoying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4
In insects, proteolytic cascades medicated by serine proteases (SPs), serine protease homologs (SPHs) and prophenoloxidases (PPOs) control several physiological processes, notably the innate immunity. However, no attempts have been made to identify and characterize these genes in Spodoptera frugiperda, one of the most destructive agricultural pests. In this study, 83 SPs, 26 SPHs and four PPOs were respectively identified in S. frugiperda genome based on homology blast against those of other insects. We then analyzed the domain organization of these proteins and assigned them into different groups by phylogenetic reconstruction. Furthermore, the mRNA levels of clip-domain SPs/SPHs (cSPs/cSPHs) and PPOs were quantified in response to a mixed infection of Micrococcus luteus and Escherichia coli, and obvious accumulations were recorded in immune tissues, including hemocytes and fat body. In the latter study, we profiled the expression patterns of highly expressed cSPs and PPOs in different developmental stages, including egg, larva, pupa, female and male adults. It was shown that most cSPs were abundantly expressed in adults, while PPOs were detected at high levels in both egg and larval stages. These current findings substantially add to our understanding of the roles of S. frugiperda SPs, SPHs and PPOs in immune regulation and further lay a solid foundation for uncovering the interaction mechanisms between insects and pathogens.
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