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      • KCI등재

        Four new species of the genus Rhagonycha (Coleoptera: Cantharidae) from Southeast China

        Xiaoyun Feng,Liu Haoyu,Yang Xing-ke,Yang Yuxia 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

        Four new species of Rhagonycha Eschscholtz, 1830 are described, including Rh. hainanensis sp. nov. (China, Hainan), Rh. nigroimpressimima sp. nov. (China, Fujian), Rh. tumida sp. nov. and Rh. nigrotibia sp. nov. (China, Guangxi). These new species are illustrated with habitus, aedeagi, abdominal sternites VIII and internal genitalia of females. Besides, the female internal genitalia of Rh. nigroimpressa (Pic, 1922a) is illustrated for the first time to make comparison with the new species. An identification key and a distribution map of those Rhagonycha species with yellowish elytra from southeast China are present.

      • KCI등재후보

        Atmospheric Corrosion of 7B04 Aluminum Alloy in Marine Environments

        ( Xiaoyun Zhang ),( Ming Liu ),( Feng Lu ),( Minghui Liu ),( Zhihua Sun ),( Zhihui Tang ) 한국부식방식학회(구 한국부식학회) 2018 Corrosion Science and Technology Vol.17 No.1

        Outdoor exposure tests using of 7B04 aluminium alloy samples including plate, tensile and various SCC samples were carried out in Tuandao station, Shandong province (East of China) and Wanning station, Hainan province (South of China). Corrosion characteristics including weight loss, microstructure, tensile strength and SCC susceptibility were investigated. The corrosion rates in Tuandao and Wanning showed high to low and the corrosion rates changed to the following equation of w=at<sup>b</sup> (b<1). The corrosion of 7B04 aluminium alloy in Wanning was more serious than that in Tuandao. Pitting appeared at early stage of expose test, and it can be changed to general corrosion with test time extension. The 7B04 aluminium alloy of which specimen shapes are forging and thick plate also showed SCC (Stress corrosion cracking) in the marine atmosphere. The higher SCC sensitivity was observed in Wanning station than in Tuandao station. The 7B04 aluminium alloy with a high stress level was more sensitive to SCC. Intergranular and transgranular or a mixed mode of cracking can be observed in different marine exposure.

      • SCIESCOPUSKCI등재

        Rapid Detection of Clostridium tetani by Recombinase Polymerase Amplification Using an Exo Probe

        ( Mingjing Guo ),( Pan Feng ),( Liqun Zhang ),( Chunfeng Feng ),( Jie Fu ),( Xiaoyun Pu ),( Fei Liu ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1

        Tetanus is a potentially fatal public health illness resulted from the neurotoxins generated by Clostridium tetani. C. tetani is not easily culturable and culturing the relevant bacteria from infected wounds has rarely been useful in diagnosis; PCR-based assays can only be conducted at highly sophisticated laboratories. Therefore, a real-time recombinase polymerase amplification assay (Exo-RPA) was constructed to identify the fragments of the neurotoxin gene of C. tetani. Primers and the exo probe targeting the conserved region were designed, and the resulting amplicons could be detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in penetrating wounds. Tests of target-spiked serum and pus extract revealed that RPA is robust to interfering factors and has great potential for further development for biological sample analysis. This method has been confirmed to be reliable for discriminating between toxic and nontoxic C. tetani strains. The RPA assay dramatically improves the diagnostic efficacy with simplified device architecture and is a promising alternative to real-time PCR for tetanus detection.

      • SCIESCOPUSKCI등재

        A Hybrid CBPWM Scheme for Single-Phase Three-Level Converters

        Wang, Shunliang,Song, Wensheng,Feng, Xiaoyun,Ding, Rongjun The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

        A novel hybrid carrier-based pulse width modulation (CBPWM) scheme that combines unipolar and dipolar modulations is proposed for single-phase three-level rectifiers, which are widely applied in railway traction drive systems. The proposed CBPWM method can satisfy the volt-second balancing principle in the complete modulation index region through overmodulation compensation. The modulation scheme features two modulation modes: unipolar and dipolar. The operation range limits of these modulation modes can be modified by changing the separation coefficient. In comparison with the traditional unipolar CBPWM, the proposed hybrid CBPWM scheme can provide advantageous features, such as lower high-order harmonic distortion of the line current and better utilization of switching frequency. The separation coefficient value is optimized to achieve the maximum utilization of these advantages. The experimental results verify the feasibility and effectiveness of the proposed hybrid CBPWM scheme.

      • KCI등재

        A Hybrid CBPWM Scheme for Single-Phase Three-Level Converters

        Shunliang Wang,Wensheng Song,Xiaoyun Feng,Rongjun Ding 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.2

        A novel hybrid carrier-based pulse width modulation (CBPWM) scheme that combines unipolar and dipolar modulations is proposed for single-phase three-level rectifiers, which are widely applied in railway traction drive systems. The proposed CBPWM method can satisfy the volt-second balancing principle in the complete modulation index region through overmodulation compensation. The modulation scheme features two modulation modes: unipolar and dipolar. The operation range limits of these modulation modes can be modified by changing the separation coefficient. In comparison with the traditional unipolar CBPWM, the proposed hybrid CBPWM scheme can provide advantageous features, such as lower high-order harmonic distortion of the line current and better utilization of switching frequency. The separation coefficient value is optimized to achieve the maximum utilization of these advantages. The experimental results verify the feasibility and effectiveness of the proposed hybrid CBPWM scheme.

      • SCOPUSKCI등재

        Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues

        Shen, Yuanming,Li, Yang,Ye, Feng,Wang, Fenfen,Wan, Xiaoyun,Lu, Weiguo,Xie, Xing Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.6

        Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA RT-qPCR studies. The aim of this study was to identify the most stable reference gene(s) for quantification of microRNA expression analysis in uterine cervical tissues. A microarray was performed on 6 pairs of uterine cervical tissues to identify the candidate reference genes. The stability of candidate reference genes was assessed by RT-qPCR in 23 pairs of uterine cervical tissues. The identified most stable reference genes were further validated in other cohort of 108 clinical uterine cervical samples: (HR-HPV- normal, n = 21; HR-HPV+ normal, n = 19; cervical intraepithelial neoplasia [CIN], n = 47; cancer, n = 21), and the effects of normalizers on the relative quantity of target miR-424 were assessed. In the array experiment, miR-26a, miR-23a, miR-200c, let-7a, and miR-1979 were identified as candidate reference genes for subsequent validation. MiR-23a was identified as the most reliable reference gene followed by miR-191. The use of miR-23a and miR-191 to normalize expression data enabled detection of a significant deregulation of miR-424 between normal, CIN and cancer tissue. Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer.

      • Evaluation of optimal ground motion intensity measures of high-speed railway train running safety on bridges during earthquakes

        Xiang Liu,Lizhong Jiang,Ping Xiang,Yulin Feng,Zhipeng Lai,Xiaoyun Sun 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.81 No.2

        Due to the large number of railway bridges along China's high-speed railway (HSR) lines, which cover a wide area with many lines crossing the seismic zone, the possibility of a HSR train running over a bridge when an earthquake occurs is relatively high. Since the safety performance of the train will be threatened, it is necessary to study the safety of trains running over HSR bridges during earthquakes. However, ground motion (GM) is highly random and selecting the appropriate groundmotion intensity measures (IMs) for train running safety analysis is not trivial. To deal this problem, a model of a coupled trainbridge system under seismic excitation was established and 104 GM samples were selected to evaluate the correlation between 16 different IMs and train running safety over HSR bridges during earthquakes. The results show that spectral velocity (SvT1) and displacement (SdT1) at the fundamental period of the structure have good correlation with train running safety for mediumand long-period HSR bridges, and velocity spectrum intensity (VSI) and Housner intensity (HI) have good correlation for a wide range of structural periods. Overall, VSI and HI are the optimal IMs for safety analysis of trains running over HSR bridges during earthquakes. Finally, based on VSI and HI, the IM thresholds of an HSR bridge at different speed were analyzed.

      • KCI등재

        Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues

        Yuanming Shen,Xing Xie,Yang Li,Feng Ye,Fenfen Wang,Xiaoyun Wan,Weiguo Lu 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.6

        Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However,few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA RT-qPCR studies. The aim of this study was to identify the most stable reference gene(s) for quantification of microRNA expression analysis in uterine cervical tissues. A microarray was performed on 6 pairs of uterine cervical tissues to identify the candidate reference genes. The stability of candidate reference genes was assessed by RT-qPCR in 23 pairs of uterine cervical tissues. The identified most stable reference genes were further validated in other cohort of 108 clinical uterine cervical samples:(HR-HPV- normal, n = 21; HR-HPV+ normal, n = 19; cervical intraepithelial neoplasia [CIN], n = 47; cancer, n = 21), and the effects of normalizers on the relative quantity of target miR-424 were assessed. In the array experiment, miR-26a, miR-23a, miR-200c, let-7a, and miR-1979 were identified as candidate reference genes for subsequent validation. MiR-23a was identified as the most reliable reference gene followed by miR-191. The use of miR-23a and miR-191 to normalize expression data enabled detection of a significant deregulation of miR-424 between normal, CIN and cancer tissue. Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer.

      • SCIESCOPUSKCI등재

        Mesocarbon microbead densified matrix graphite A3-3 for fuel elements in molten salt reactors

        Wang, Haoran,Xu, Liujun,Zhong, Yajuan,Li, Xiaoyun,Tang, Hui,Zhang, Feng,Yang, Xu,Lin, Jun,Zhu, Zhiyong,You, Yan,Lu, Junqiang,Zhu, Libing Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.5

        This study aims to provide microstructural characterization for the matrix graphite which molten salt reactors (MSRs) use, and improve resistance to molten salt infiltration of the matrix graphite for fuel elements. Mesocarbon microbeads (MCMB) densified matrix graphite A3-3 (MDG) was prepared by a quasi-isostatic pressure process. After densification by MCMBs with average particle sizes of 2, 10, and 16 ㎛, the pore diameter of A3-3 decreased from 924 nm to 484 nm, 532 nm, and 778 nm, respectively. Through scanning electron microscopy, the cross-section energy spectrum and time-of-flight secondary ion mass spectrometry, resistance levels of the matrix graphite to molten salt infiltration were analyzed. The results demonstrate that adding a certain proportion of MCMB powders can improve the anti-infiltration ability of A3-3. Meanwhile, the closer the particle size of MCMB is to the pore diameter of A3-3, the smaller the average pore diameter of MDG and the greater the densification. As a matrix graphite of fuel elements in MSR was involved, the thermal and mechanical properties of matrix graphite MDG were also studied. When densified by the MCMB matrix graphite, MDGs can meet the molten salt anti-infiltration requirements for MSR operation.

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