An Improved DV-Hop Localization Algorithm Based on Hop Distance and Hops Correction

Xiaoying Yang,Wanli Zhang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6

In order to solve the problem that the DV-Hop algorithm has large error in the network topology environment of random distribution of nodes, this paper proposes a WSN localization algorithm based on hop distance correction. The algorithm uses the method of multi communication radius to correct the hop number within 1 hops, which makes it more consistent with the real situation; Then, the weighted factor is obtained by error of the average distance per hop of anchor node; Finally, the total least squares method is used to calculate the coordinates of the unknown nodes to improve the positioning accuracy of DV-Hop positioning algorithm. The simulation results show that the improved algorithm has a better accuracy and stability than the original DV-Hop algorithm.

A Temperature Control System Design Based on 51 SCM

Xiaoying Yang,Wanli Zhang,Qixiang Song 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.4

Temperature control is quite extensive in their daily lives and industrial applications, and past temperature control is done by hand and not enough attention, in fact, temperature needs to be monitored in many places in order to prevent accidents. To solve this problem, a temperature control system based on 51 Single Chip Microcomputer (SCM) is designed in the paper. Combining with SCM AT89C51, temperature sensor is used to collect temperature, the collected temperature values are transferred to the LED display to shows. Then cooling or heating is automatically operated by the fan or an electric wire in order to achieve a constant temperature. The experimental results show that the system can perform real-time monitoring of temperature and reach the goal of effectively controlling temperature.

Multi-scale and Interactive Visual Analysis of Public Bicycle System

( Xiaoying Shi ),( Yang Wang ),( Fanshun Lv ),( Xiaohang Yang ),( Qiming Fang ),( Li Zhang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.6

Public bicycle system (PBS) is a new emerging and popular mode of public transportation. PBS data can be adopted to analyze human movement patterns. Previous work usually focused on specific scales, and the relationships between different levels of hierarchies are ignored. In this paper, we introduce a multi-scale and interactive visual analytics system to investigate human cycling movement and PBS usage condition. The system supports level-of-detail explorative analysis of spatio-temporal characteristics in PBS. Visual views are designed from global, regional and microcosmic scales. For the regional scale, a bicycle network is constructed to model PBS data, and an flow-based community detection algorithm is applied on the bicycle network to determine station clusters. In contrast to the previous used Louvain algorithm, our method avoids producing super-communities and generates better results. We provide two cases to demonstrate how our system can help analysts explore the overall cycling condition in the city and spatio-temporal aggregation of stations.

Ginsenosides Rc, as a novel SIRT6 activator, protects mice against high fat diet induced NAFLD

Zehong Yang,Yuanyuan Yu,Nannan Sun,Limian Zhou,Dong Zhang,HaiXin Chen,Wei Miao,Weihang Gao,Canyang Zhang,Changhui Liu,Xiaoying Yang,Xiaojie Wu,Yong Gao The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.3

Background: Hepatic lipid disorder impaired mitochondrial homeostasis and intracellular redox balance, triggering development of non-alcohol fatty liver disease (NAFLD), while effective therapeutic approach remains inadequate. Ginsenosides Rc has been reported to maintain glucose balance in adipose tissue, while its role in regulating lipid metabolism remain vacant. Thus, we investigated the function and mechanism of ginsenosides Rc in defending high fat diet (HFD)-induced NAFLD. Methods: Mice primary hepatocytes (MPHs) challenged with oleic acid & palmitic acid were used to test the effects of ginsenosides Rc on intracellular lipid metabolism. RNAseq and molecular docking study were performed to explore potential targets of ginsenosides Rc in defending lipid deposition. Wild type and liver specific sirtuin 6 (SIRT6, 50721) deficient mice on HFD for 12 weeks were subjected to different dose of ginsenosides Rc to determine the function and detailed mechanism in vivo. Results: We identified ginsenosides Rc as a novel SIRT6 activator via increasing its expression and deacetylase activity. Ginsenosides Rc defends OA&PA-induced lipid deposition in MPHs and protects mice against HFD-induced metabolic disorder in dosage dependent manner. Ginsenosides Rc (20mg/kg) injection improved glucose intolerance, insulin resistance, oxidative stress and inflammation response in HFD mice. Ginsenosides Rc treatment accelerates peroxisome proliferator activated receptor alpha (PPAR-α, 19013)-mediated fatty acid oxidation in vivo and in vitro. Hepatic specific SIRT6 deletion abolished ginsenoside Rc-derived protective effects against HFD-induced NAFLD. Conclusion: Ginsenosides Rc protects mice against HFD-induced hepatosteatosis by improving PPAR-α-mediated fatty acid oxidation and antioxidant capacity in a SIRT6 dependent manner, and providing a promising strategy for NAFLD.

Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae

Liu Yang,Ce Shi,Xiaoying Mu,Chao Liu,Ke Shi,Wen-jiao Zhu,Qing Yang 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4

Plant P450s play an important role in plant response to environmental stimuli, especially to pathogen challenge, besides leading to the biosynthesis of secondary metabolites. In this work, a Verticillium dahliae-induced cDNA sequence was cloned from wild eggplant Solanum torvum and designated StoCYP77A2. StoCYP77A2 encodes a 511-amino acid peptide that shows sequence homology to the plant CYP77 family. StoCYP77A2 is expressed in a tissue-specific manner with the highest expression level in stem. The expression level of StoCYP77A2 was increased in wild eggplants which were infected with Verticillium dahlia. We expressed StoCYP77A2 in V. dahliae-sensitive tobacco, and the transgenic tobacco plants were more resistant to V. dahliae infection than the wild type. Protein extraction of transgenic tobacco showed strong antifungal activity against V. dahliae, indicating that StoCYP77A2 may be involved in the synthesis of some antimicrobial compounds. These results suggest that StoCYP77A2 plays a role in plant defense to V. dahliae infection.

Development of a Malignancy Potential Binary Prediction Model Based on Deep Learning for the Mitotic Count of Local Primary Gastrointestinal Stromal Tumors

Yang Jiejin,Chen Zeyang,Liu Weipeng,Wang Xiangpeng,Ma Shuai,Jin Feifei,Wang Xiaoying 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.3

Objective: The mitotic count of gastrointestinal stromal tumors (GIST) is closely associated with the risk of planting and metastasis. The purpose of this study was to develop a predictive model for the mitotic index of local primary GIST, based on deep learning algorithm. Materials and Methods: Abdominal contrast-enhanced CT images of 148 pathologically confirmed GIST cases were retrospectively collected for the development of a deep learning classification algorithm. The areas of GIST masses on the CT images were retrospectively labelled by an experienced radiologist. The postoperative pathological mitotic count was considered as the gold standard (high mitotic count, > 5/50 high-power fields [HPFs]; low mitotic count, ≤ 5/50 HPFs). A binary classification model was trained on the basis of the VGG16 convolutional neural network, using the CT images with the training set (n = 108), validation set (n = 20), and the test set (n = 20). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated at both, the image level and the patient level. The receiver operating characteristic curves were generated on the basis of the model prediction results and the area under curves (AUCs) were calculated. The risk categories of the tumors were predicted according to the Armed Forces Institute of Pathology criteria. Results: At the image level, the classification prediction results of the mitotic counts in the test cohort were as follows: sensitivity 85.7% (95% confidence interval [CI]: 0.834–0.877), specificity 67.5% (95% CI: 0.636–0.712), PPV 82.1% (95% CI: 0.797–0.843), NPV 73.0% (95% CI: 0.691–0.766), and AUC 0.771 (95% CI: 0.750–0.791). At the patient level, the classification prediction results in the test cohort were as follows: sensitivity 90.0% (95% CI: 0.541–0.995), specificity 70.0% (95% CI: 0.354–0.919), PPV 75.0% (95% CI: 0.428–0.933), NPV 87.5% (95% CI: 0.467–0.993), and AUC 0.800 (95% CI: 0.563–0.943). Conclusion: We developed and preliminarily verified the GIST mitotic count binary prediction model, based on the VGG convolutional neural network. The model displayed a good predictive performance.

First-principles study of Ti doping in FeF3$0.33H2O

Zhenhua Yang,Zhijuan Zhang,Yalong Yuan,Yunqing Huang,Xianyou Wang,Xiaoying Chen,Shuangying Wei 한국물리학회 2016 Current Applied Physics Vol.16 No.8

The effect of Ti doping on the geometrical and electronic structures of FeF3$0.33H2O are systematically investigated by using the first principles calculations. We focused on TixFe1-xF3$0.33H2O systems, in which x is equal to 0, 0.08, 0.17 and 0.25, respectively. Different kinds of Ti dopant sites are checked and the most stable structure can be obtained by comparison of total energy. The crystal volume of TixFe1- xF3$0.33H2O expands gradually with increasing Ti doping concentration. Calculated formation energies indicate TixFe1-xF3 is easiest to fabricate and the difficulty of Ti doping FeF3 with hexagonal-tungstenbronze( HTB) structure decreases with the increase of Ti doping concentration under the Fe-rich and Ti-rich growth conditions. Moreover, TixFe1-xF3$0.33H2O is thermodynamically stable, indicating that water molecule can preferentially occupy one-dimensional cavity in the TixFe1-xF3. The band gap of TixFe1-xF3$0.33H2O decreases with increasing Ti doping concentration and Ti0.25Fe0.75F3$0.33H2O exhibits character of half metal, indicating that the conductivity of FeF3$0.33H2O can be improved by Ti-doping. Besides, it can be confirmed that Ti-doping also can broaden the hexagonal cavity in the FeF3$0.33H2O by analyzing the crystal structure of FeF3$0.33H2O and TixFe1-xF3$0.33H2O. With excellent conductivity and larger hexagonal cavity, TixFe1-xF3$0.33H2O can afford open diffusion channels. Therefore, Li ions can remain unblocked, which is beneficial to fast charge and discharge.

Overexpressing osa-miR171c decreases salt stress tolerance in rice

Wu Yang,Tian Fan,Xiaoying Hu,Taihui Cheng,Mingyong Zhang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

The miRNA171 family is one of the well-conservedmiRNA families, and its role under stresses is not knownexcept its expression on genome-wide expression analyses. osa-miR171c was induced by high concentration of salt (150mM NaCl). A rice dh mutant with osa-miR171c overexpressiontriggered by a T-DNA insertion, significantly decreased salttolerance at the stages of germination and seedling. Thisphenotype was confirmed by osa-miR171c overexpressiontransgenic rice. Compared with wild-type (WT), dh mutantreduced amounts of free proline and increased the water lossrate after salt treatment. Stomatal density in the leafepidermis of dh mutant also increased. Moreover, dh mutantincreased sensitivity to ABA treatment. Several stressresponsivegenes were down-regulated in dh mutant than inWT under salty stress. These results indicate that osamiR171cis involved in modulating physiological changes,stomatal development, ABA-dependent pathways and expressionof stress-related genes; thereby, it possibly contributes tosalty tolerance.

Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope

Wang Xiaoxia,Yang Jing,Huang Yuyuan,Wu Xiaomin,Wang Licheng,Han Limei,Li Sha,Li Huan,Fu Xiaoying,Chen Hai,Zhu Xiong 한국미생물학회 2020 The journal of microbiology Vol.58 No.9

Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423T and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423T and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612T and Georgenia subflava CGMCC 1.12782T. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612T and Georgenia wutianyii Z294T. The predominant cellular fatty acids were anteiso-C15:0, anteiso-C15:1 A and C16:0. The major menaquinone was MK-8(H4). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA–DNA hybridization value between strains ZLJ0423T and ZLJ0321 was 91.2%, but their values with closely related species and other available type strains of the genus Georgenia were lower than the 70% threshold. On the basis of polyphasic taxonomic data, strains ZLJ0423T and ZLJ0321 represent a novel species within the genus Georgenia, for which the name Georgenia faecalis sp. nov. is proposed. The type strain is ZLJ0423T (= CGMCC 1.13681T = JCM 33470T).

Circ_0000554 is identified as a cancer‑promoting circRNA in colorectal cancer by regulating the miR‑1205/LASP1 axis

Luo Jinlong,Yang Hua,Peng Xuefeng,Zhang Faqiang,Shu Shilong,Lan Ke,Tu Shengjin,Lu Kai,Cha Xiaoying 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5

Background: Colorectal cancer (CRC) is a prevalent malignant tumor with poor prognosis. Circular RNAs (circRNAs) are key regulators in the progression of CRC. Our study aimed to disclose the role of circ_0000554 in CRC. Methods: The expression of circ_0000554, miR-1205 and LIM and SH3 protein 1 (LASP1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, invasion and migration were monitored using cell counting kit-8 (CCK-8) assay, EdU assay, transwell assay and wound healing assay respectively. The protein levels of C-myc, matrix metallopeptidase 2 (MMP-2) and LASP1 were detected by western blot. Tumor formation assay in nude mice was conducted to explore the role of circ_0000554 in vivo. The association between miR-1205 and circ_0000554 or LASP1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Results: circ_0000554 was upregulated in CRC tissues and cells, high circ_0000554 expression was significantly linked to shorter overall survival. Downregulation of circ_0000554 restrained cell growth and metastasis while promoted apoptosis in vitro, and suppressed tumorigenesis of CRC in vivo. Furthermore, mechanism study and rescue experiments confirmed miR-1205 could be sponged by circ_0000554 and its inhibitor reversed the inhibitory effect of circ_0000554 silencing on CRC progression. LASP1 was a target gene of miR-1205 and the upregulation of LASP1 overturned miR-1205-induced effects on CRC cells. Circ_0000554 could elevate LASP1 expression via interacting with miR-1205.