Improved Fluorescence of Fluorene Polymer in Solid State

Xiaoyan Zhao,Shuai Zhang,Chen Huang,Chenyi Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.10

Fluorene-containing poly(aryl ether nitrile) (FPAN) was prepared by a typically aromatic nucleophilic substitutionpolymerization. The chemical structure of FPAN was confirmed by FTIR and 1H NMR spectroscopy. Different morphologiesof FPAN fibers could obtain through electrospinning. The light-emission properties of polymer in solution, casting film andfibrous membrane were examined by spectrofluorometry. This polymer had good thermal properties with glass-transitiontemperatures at 280 oC and 10 % weight loss temperatures in the range of 497-502 oC. Additionally, the contact angles in thethin-film state and electrospun fiber membranes were examined. The water contact angle of fiber membrane was improvedcompared to that of casting film which could reach to the 137 o. The resulting material exhibited stable luminescent intensity,possessed considerable thermal stability, and could be facilely processed into device for optical field applications.

Family History of Cancer and Head and Neck Cancer Risk in a Chinese Population

Huang, Yu-Hui Jenny,Lee, Yuan-Chin Amy,Li, Qian,Chen, Chien-Jen,Hsu, Wan-Lun,Lou, Pen-Jen,Zhu, Cairong,Pan, Jian,Shen, Hongbing,Ma, Hongxia,Cai, Lin,He, Baochang,Wang, Yu,Zhou, Xiaoyan,Ji, Qinghai,Zho Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: The aim of this study was to investigate whether family history of cancer is associated with head and neck cancer risk in a Chinese population. Materials and Methods: This case-control study included 921 cases and 806 controls. Recruitment was from December 2010 to January 2015 in eight centers in East Asia. Controls were matched to cases with reference to sex, 5-year age group, ethnicity, and residence area at each of the centers. Results: We observed an increased risk of head and neck cancer due to first degree family history of head and neck cancer, but after adjustment for tobacco smoking, alcohol drinking and betel quid chewing the association was no longer apparent. The adjusted OR were 1.10 (95% CI=0.80-1.50) for family history of tobacco-related cancer and 0.96 (95%CI=0.75-1.24) for family history of any cancer with adjustment for tobacco, betel quid and alcohol habits. The ORs for having a first-degree relative with HNC were higher in all tobacco/alcohol subgroups. Conclusions: We did not observe a strong association between family history of head and neck cancer and head and neck cancer risk after taking into account lifestyle factors. Our study suggests that an increased risk due to family history of head and neck cancer may be due to shared risk factors. Further studies may be needed to assess the lifestyle factors of the relatives.

SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco

Xiaoyan Hao,Jianping Li,Shengqi Gao,Zumuremu Tuerxun,Xiaochun Chang,Wenran Hu,Guo Chen,Quansheng Huang 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality. Objective: To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance. Methods: Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method. Results: A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines. Conclusions: These results show that SsPsaH may contribute to the salt and osmotic stress response of plants.

The Role of Neutrophil-to-Lymphocyte Ratio in Predicting Pathological Response for Resectable Non–Small Cell Lung Cancer Treated with Neoadjuvant Chemotherapy Combined with PD-1 Checkpoint Inhibitors

Xiaoyan Sun,Yingnan Feng,Bin Zhang,Wuhao Huang,Xiaoliang Zhao,Hua Zhang,Dongsheng Yue,Changli Wang 대한암학회 2022 Cancer Research and Treatment Vol.54 No.4

Purpose The aim of our study was to investigate the value of baseline and preoperative neutrophil-to-lymphocyte ratio (NLR) in predicting the pathological response and disease-free survival (DFS) of neoadjuvant chemotherapy alone or combined with programmed cell death-1 (PD-1) checkpoint inhibitors in patients with resectable non–small cell lung cancer (NSCLC). Materials and Methods Resectable NSCLC patients who underwent neoadjuvant chemotherapy alone or combined with PD-1 checkpoint inhibitors between January 2018 and January 2020 were included. Peripheral venous blood samples of the patients were collected within 3 days prior to the first neoadjuvant treatment and within 3 days prior to surgery. Results A total of 79 patients in neoadjuvant chemotherapy combined with PD-1 checkpoint inhibitors group and 89 patients in neoadjuvant chemotherapy alone group were included. Thirty-five point four percent of the patients achieved pathological complete response (pCR) in neoadjuvant chemotherapy combined with PD-1 checkpoint inhibitors group, whereas only 9.0% reached pCR in the group of neoadjuvant chemotherapy. High NLR level were correlated with poor pathological response and DFS in neoadjuvant chemotherapy or combined with PD-1 checkpoint inhibitors group. Multivariate analysis revealed that baseline NLR could independently predict pathological response and DFS in the neoadjuvant chemotherapy combined with PD-1 checkpoint inhibitors group. Conclusion High NLR level were correlated with poor pathological response and shorter DFS in patients with NSCLC undergoing neoadjuvant chemotherapy or combined with PD-1 checkpoint inhibitors. Meanwhile, baseline NLR could independently predict response to pathological response and DFS, revealing its potential as a screening tool in NSCLC patients who received neoadjuvant chemotherapy combined with PD-1 checkpoint inhibitors.

The Second-order Scattering of the Interaction of Pd Nanoparticles with Protein and Its Analytical Application

Xiaoyan Guo,Baolin He,Chuntao Sun,Yanxi Zhao,Tao Huang*,Kongyong Liew,Hanfan Liu 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.10

The second-order scattering (SOS) phenomenon of the interaction of Pd nanoparticles with protein was reported and a simple, sensitive, palladium nanoparticle-based assay for trace amount of protein with SOS technique was developed. The SOS intensities were significantly enhanced due to the interaction of Pd nanoparticles with bovine serum albumin (BSA) or human serum albumin (HSA) at pH 3.5 or 4.0, respectively. The maximum SOS peak appeared at 260/520 nm (lex/lem). The optimal experiment conditions, affecting factors and the influence of some coexisting substances were checked. The SOS intensity increased proportionally with the increase of Pd concentration below 3.0?´?10-5 molL-1, while declined gradually above 4.0 ´ 10-5 molL-1. BSA within the range of 0.01-2.6 mgmL-1 and HSA of 0.01-1.7 mgmL-1 can be detected with this method and the detection limits were 2.3 and 11.2 ngmL-1, respectively. The method was successfully applied to the quantitative detection of total protein content in human serum samples with the maximum relative standard deviation (RSD) lower than 2.6% and the recoveries over the range of 99.5-100.5%.

Curcumin suppresses oxidative stress via regulation of ROS/NF-κB signaling pathway to protect retinal vascular endothelial cell in diabetic retinopathy

Huang Jiang,Yi Quanyong,You Yuhong,Chen Yao,Niu Tongtong,Yi Li,Zhang Ji,Ji Xiaoyan,Xu Guoxu,Zou Weijie,Ji Fangfang,Luo Weifeng 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3

Background The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance. Objective The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry. Results We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin. Conclusion We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR. Background The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance. Objective The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry. Results We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin. Conclusion We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR.

Effect of irradiation temperature on the nanoindentation behavior of P92 steel with thermomechanical treatment

Huang Xi,Shen Yinzhong,Li Qingshan,Li Xiaoyan,Zhan Zixiong,Li Guang,Li Zhenhe 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7

The nanoindentation behavior of P92 steel with thermomechanical treatment under 3.5 MeV Fe13þ ion irradiation at room temperature, 400 and 700 C was investigated. Pop-in behavior is observed for all the samples with and without irradiation at room temperature, while the temperature dependence of pop-in behavior is only observed in irradiated samples. The average load and penetration depth at the onset of pop-in increase as the irradiation temperature increases, in line with the results of the maximum shear stress. Irradiation induced hardening is exhibited for all irradiated samples, but there is a significant reduction in the hardness of sample irradiated at 700 C in comparison to the samples irradiated at room temperature and 400 C. The ratio of hardness to elastic modulus for all samples decreases with increasing penetration depth except for samples at 700 C. With the increasing of irradiation temperature, the ratio of the irreversible work to the total work gradually decreases. In contrast, it increases for samples without irradiation

Effect of Precipitate State on Mechanical Properties, Corrosion Behavior, and Microstructures of Al–Zn–Mg–Cu Alloy

Xiaoyan Peng,Yao Li,Guofu Xu,Jiwu Huang,Zhimin Yin 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.5

The mechanical properties, corrosion behavior and microstructures of the Al–Zn–Mg–Cu alloy under various ageing treatmentswere investigated comparatively. The results show that the tensile strength and corrosion resistance are stronglyaff ected by the precipitate state. Massive fi ne intragranular precipitates contribute to high strength. Discontinuous coarsegrain boundary precipitates containing high Cu content, as well as the narrow precipitate free zone, result in low corrosionsusceptibility. After the non-isothermal ageing (NIA) treatment, the tensile strength of 577 MPa is equivalent to that of579 MPa for the T6 temper. Meanwhile, the stress corrosion susceptibility r tf and the maximum corrosion depth are 97.8%and 23.5 μm, which are comparable to those of 92.8% and 26.7 μm for the T73 temper. Moreover, the total ageing timeof the NIA treatment is only 7.25 h, which is much less than that of 48.67 h for the retrogression and re-ageing condition.

Characterization of a Thermostable Lichenase from Bacillus subtilis B110 and Its Effects on β-Glucan Hydrolysis

( Zhen Huang ),( Guorong Ni ),( Fei Wang ),( Xiaoyan Zhao ),( Yunda Chen ),( Lixia Zhang ),( Mingren Qu ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.4

Lichenase is an enzyme mainly implicated in the degradation of polysaccharides in the cell walls of grains. Emerging evidence shows that a highly efficient expression of a thermostable recombinant lichenase holds considerable promise for application in the beer-brewing and animal feed industries. Herein, we cloned a lichenase gene (CelA203) from Bacillus subtilis B110 and expressed it in E. coli. This gene contains an ORF of 729 bp, encoding a protein with 242 amino acids and a calculated molecular mass of 27.3 kDa. According to the zymogram results, purified CelA203 existed in two forms, a monomer, and a tetramer, but only the tetramer had potent enzymatic activity. CelA203 remained stable over a broad pH and temperature range and retained 40% activity at 70℃ for 1 h. The K_m and V_max of CelA203 towards barley β-glucan and lichenan were 3.98 mg/ml, 1017.17 U/mg, and 2.78 mg/ml, 198.24 U/mg, respectively. Furthermore, trisaccharide and tetrasaccharide were the main products obtained from CelA203-mediated hydrolysis of deactivated oat bran. These findings demonstrate a promising role for CelA203 in the production of oligosaccharides in animal feed and brewing industries.

The Second-order Scattering of the Interaction of Pd Nanoparticles with Protein and Its Analytical Application

Guo, Xiaoyan,He, Baolin,Sun, Chuntao,Zhao, Yanxi,Huang, Tao,Liew, Kongyong,Liu, Hanfan Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.10

The second-order scattering (SOS) phenomenon of the interaction of Pd nanoparticles with protein was reported and a simple, sensitive, palladium nanoparticle-based assay for trace amount of protein with SOS technique was developed. The SOS intensities were significantly enhanced due to the interaction of Pd nanoparticles with bovine serum albumin (BSA) or human serum albumin (HSA) at pH 3.5 or 4.0, respectively. The maximum SOS peak appeared at 260/520 nm (λex/λem). The optimal experiment conditions, affecting factors and the influence of some coexisting substances were checked. The SOS intensity increased proportionally with the increase of Pd concentration below 3.0 × 10?5 mol·L?1, while declined gradually above 4.0 × 10?5 mol·L?1. BSA within the range of 0.01-2.6 μg·mL?1 and HSA of 0.01-1.7 μg·mL?1 can be detected with this method and the detection limits were 2.3 and 11.2 ng·mL?1, respectively. The method was successfully applied to the quantitative detection of total protein content in human serum samples with the maximum relative standard deviation (RSD) lower than 2.6% and the recoveries over the range of 99.5-100.5%.