HGAL, a germinal center specific protein, decreases lymphoma cell motility by modulation of the RhoA signaling pathway

Jiang, Xiaoyu,Lu, Xiaoqing,McNamara, George,Liu, Xiaofei,Cubedo, Elena,Sarosiek, Kristopher A.,Sá,nchez-Garcí,a, Isidro,Helfman, David M.,Lossos, Izidore S. American Society of Hematology 2010 Blood Vol.116 No.24

<B>Abstract</B><P>HGAL is a germinal center (GC)-specific gene that negatively regulates lymphocyte motility and whose expression predicts improved survival of patients with diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin lymphoma (cHL). We demonstrate that HGAL serves as a regulator of the RhoA signaling pathway. HGAL enhances activation of RhoA and its down-stream effectors by a novel mechanism - direct binding to the catalytic DH-domain of the RhoA-specific guanine nucleotide exchange factors (RhoGEFs) PDZ-RhoGEF and LARG that stimulate the GDP-GTP exchange rate of RhoA. We delineate the structural domain of HGAL that mediates its interaction with the PDZ-RhoGEF protein. These observations reveal a novel molecular mechanism underlying the inhibitory effects of GC-specific HGAL protein on the motility of GC-derived lymphoma cells. This mechanism may underlie the limited dissemination and better outcome of patients with HGAL-expressing DLBCL and cHL.</P>

CircRNA circ_0067772 aggravates the malignant progression of cutaneous squamous cell carcinoma by regulating miR‑1238‑3p/ FOXG1 axis

Xiaoqing Li,Yinghui Kong,He Li,Manyuan Xu,Ming Jiang,Weiguo Sun,Suping Xu 한국유전학회 2021 Genes & Genomics Vol.43 No.5

Background Cutaneous squamous cell carcinoma (CSCC) is a severe malignancy derived from skin. Dysregulated circular RNAs (circRNAs) might play vital roles in tumor development. Objective Here, we aimed to explore the function of a novel circRNA circ_0067772 in CSCC. Methods Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to determine the expression of circ_0067772, microRNA (miR)-1238-3p and forkhead box protein G1 (FOXG1). Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Transwell assay and wound healing assay were employed to examine cell metastasis. Flow cytometry was employed to monitor cell cycle and apoptosis. The target association between miR-1238-3p and circ_0067772 or FOXG1 was validated by dual-luciferase reporter assay. Moreover, role of circ_0067772 in vivo was investigated via xenograft model in nude mice. Results Circ_0067772 and FOXG1 were upregulated, while miR-1238-3p was downregulated in CSCC tissues and cells. Circ_0067772 knockdown conferred inhibitory efects on cell proliferation, migration and invasion of CSCC cells. MiR1238-3p served as a target of circ_0067772, whose silencing could reverse circ_0067772 knockdown-induced inhibitory impact on the malignant cellular behaviors. Circ_0067772 positively regulated FOXG1 expression by antagonizing miR1238-3p. Additionally, miR-1238-3p could repress CSCC cell proliferation, migration and invasion by targeting FOXG1. Also, circ_0067772 knockdown hindered CSCC tumor growth in vivo. Conclusion Our study identifed a novel oncogenic circRNA and the involvement of circ_0067772/miR-1238-3p/FOXG1 axis in CSCC development, providing a target for CSCC therapy.

Experimental study on wake-induced vibrations of two circular cylinders with two degrees of freedom

Xiaoqing Du,Benjian Jiang,Chin Dai,Guoyan Wang,Suren Chen 한국풍공학회 2018 Wind and Structures, An International Journal (WAS Vol.26 No.2

Wind tunnel tests are conducted to investigate wake-induced vibrations of two circular cylinders with a center-to-center spacing of 4 diameters and attack angle varying from 0 to 20 for Reynolds numbers between 18,000 and 168,800. Effects of structural damping, Reynolds number, attack angle and reduced velocity on dynamic responses are examined. Results show that wake-induced vortex vibrations of the downstream cylinder occur in a wider range of the reduced velocity and have higher amplitudes in comparison to the vortex-induced vibration of a single circular cylinder. Two types of wake-induced instability phenomena with distinct dynamic characteristics are observed, which may be due to different generation mechanisms. For small attack angles like 5 and 10, the instability of the downstream cylinder characterizes a one-degree-of-freedom (1-DOF) oscillation moving in the across-wind direction. For a large attack angle like 20, the instability characterizes a two-degree-of-freedom (2-DOF) oscillation with elliptical trajectories. For an attack angle of 15, the instability can transform from the 1-DOF pattern to the 2-DOF one with the increase of the Reynolds number. Furthermore, the two instabilities show different sensitivity to the structural damping. The 1-DOF instability can be either completely suppressed or reduced to an unsteady oscillation, while the 2-DOF one is relatively less sensitive to the damping level. Reynolds number has important effects on the wake-induced instabilities.

CHIRAL CARBON NANOTUBES AND CARBON NANOTUBE CHIRAL COMPOSITES:PREPARATION AND APPLICATIONS

XIAOQING CHEN,XIAO-YUN LIAO,JIN-GANG YU,FEI-PENG JIAO,XINYU JIANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.4

A review of preparation of chiral carbon nanotubes (CNTs) and carbon nanotube chiral composites is presented. Novel chiral separation science and technology based on CNTs is analyzed and discussed, with the emphasis in two topics. The first topic concerns the possibility of application of chiral CNTs in resolution and chiral sensing. The second topic centers on the subject of enantioseparation based on CNT chiral composites. Despite the crucial functionalization of CNTs using chiral selectors, better resolution could also be achieved by using a mixture of CNTs and optical pure substances as chiral selectors. Due to their ability of improving the degree of enantioseparation, CNTs could find their potential applications in the resolution in the future.

Reusable, magnetic laser-induced graphene for efficient removal of organic pollutants from water

Jiang Ye,Wan Sijie,Zhao Weiwei,Yu Wenjie,Wang Shuaipeng,Yu Zeqi,Yang Qiu,Zhou Weihua,Liu Xiaoqing 한국탄소학회 2022 Carbon Letters Vol.32 No.4

The hybridization of graphene with magnetic nanoparticles has endowed graphene with increasing interest as the adsorbent for wastewater treatment. However, its fabrication often involves a multi-stepped chemical synthesis process. In this work, we demonstrate a facile, one-step, and solvent-free approach to fabricate Fe3O4 nanoparticle-anchored Laser-Induced Graphene (Fe3O4@LIG) as an efficient adsorbent by direct laser irradiation on a ferric acetylacetonate containing polybenzoxazine film. Raman and X-ray diffraction analysis confirm the graphene component in the adsorbent, and the morphology characterizations show that Fe3O4 nanoparticles are distributed uniformly on LIG with hierarchical meso- and macro-porous structures. Adsorption experiments indicate that Fe3O4@LIG can adsorb methylene blue (MB) from aqueous solutions in a fast and effective manner, with a maximum adsorption capacity up to 350.9 mg/g. The adsorption kinetics and isotherms are also investigated, which are well-described by the pseudo-second-order model and Langmuir model, respectively. Additionally, Fe3O4@LIG is also demonstrated with the efficient removal of a variety of organic solvents from water. The favorable adsorption behavior of Fe3O4@LIG is attributed to its unique porous structure and the molecular interactions with adsorbates. On the other hand, Fe3O4@LIG has high magnetic property, and therefore, it could be easily recovered from water and well regenerated for repeated use. With the efficient adsorption of organic pollutants, magnetic separability, and good recyclability, it is believed that the easy-fabricated Fe3O4@LIG has great potential applications in wastewater treatment.

Germinal center‐specific protein human germinal center associated lymphoma directly interacts with both myosin and actin and increases the binding of myosin to actin

Lu, Xiaoqing,Kazmierczak, Katarzyna,Jiang, Xiaoyu,Jones, Michelle,Watt, James,Helfman, David M.,Moore, Jeffrey R.,Szczesna‐,Cordary, Danuta,Lossos, Izidore S. Blackwell Publishing Ltd 2011 The FEBS journal Vol.278 No.11

<P>Human germinal center associated lymphoma (HGAL) is a germinal center‐specific gene whose expression correlates with a favorable prognosis in patients with diffuse large B‐cell and classic Hodgkin lymphomas. HGAL is involved in negative regulation of lymphocyte motility. The movement of lymphocytes is directly driven by actin polymerization and actin–myosin interactions. We demonstrate that HGAL interacts directly and independently with both actin and myosin and delineate the HGAL and myosin domains responsible for the interaction. Furthermore, we show that HGAL increases the binding of myosin to F‐actin and inhibits the ability of myosin to translocate actin by reducing the maximal velocity of myosin head/actin movement. No effects of HGAL on actomyosin ATPase activity and the rate of actin polymerization from G‐actin to F‐actin were observed. These findings reveal a new mechanism underlying the inhibitory effects of germinal center‐specific HGAL protein on lymphocyte and lymphoma cell motility.</P>

Experimental study on wake-induced vibrations of two circular cylinders with two degrees of freedom

Du, Xiaoqing,Jiang, Benjian,Dai, Chin,Wang, Guoyan,Chen, Suren Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.26 No.2

Wind tunnel tests are conducted to investigate wake-induced vibrations of two circular cylinders with a center-to-center spacing of 4 diameters and attack angle varying from $0^{\circ}$ to $20^{\circ}$ for Reynolds numbers between 18,000 and 168,800. Effects of structural damping, Reynolds number, attack angle and reduced velocity on dynamic responses are examined. Results show that wake-induced vortex vibrations of the downstream cylinder occur in a wider range of the reduced velocity and have higher amplitudes in comparison to the vortex-induced vibration of a single circular cylinder. Two types of wake-induced instability phenomena with distinct dynamic characteristics are observed, which may be due to different generation mechanisms. For small attack angles like $5^{\circ}$ and $10^{\circ}$, the instability of the downstream cylinder characterizes a one-degree-of-freedom (1-DOF) oscillation moving in the across-wind direction. For a large attack angle like $20^{\circ}$, the instability characterizes a two-degree-of-freedom (2-DOF) oscillation with elliptical trajectories. For an attack angle of $15^{\circ}$, the instability can transform from the 1-DOF pattern to the 2-DOF one with the increase of the Reynolds number. Furthermore, the two instabilities show different sensitivity to the structural damping. The 1-DOF instability can be either completely suppressed or reduced to an unsteady oscillation, while the 2-DOF one is relatively less sensitive to the damping level. Reynolds number has important effects on the wake-induced instabilities.

Removal of gaseous toluene by nonthermal plasma coupled with wet scrubber containing Fe2+

Chaochao Jiang,Caihong Qin,Mengke Guo,Jiayu Huang,Dongjie Yan,Xiaoqing Dang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

A non-thermal plasma with a wet scrubber (NTP + WS) was used to degrade gaseous toluene. Comparedwith the NTP + WS/H2O system, the toluene and by-product ozone removal efficiencies were increased by10% and 85% in the NTP + WS/Fe2+ system, respectively. For 60 ppm toluene degradation, the maximummineralization rate (MR) of toluene (61%) was obtained, at the optimum Fe2+ concentration of 50 mg/L. During catalytic oxidation, the removal efficiency of toluene was limited by its solubility in WS systems. In addition, decreased Fe2+ concentration and accumulation of organics led to the system deactivation. The EPR and quenching experiments verified the presence and contribution of hydroxyl radicals (4.6%)and superoxide radicals (3.8%) to the removal efficiency of toluene. Based on the radicals and intermediatesdetected in the gas and liquid phases, a degradation mechanism for toluene in the NTP + WS/Fe2+system was proposed.

A Visual Study of Effect of Heating on Burst of Turbulent Spots

Li Yang,Xiaoqing Jiang,Fuguo Zhuo,Xianzhi Du 대한기계학회 1998 Heat transter conference Vol.3 No.1

INVESTIGATION OF OCCUPANT LOWER EXTREMITY INJURES UNDER VARIOUS OVERLAP FRONTAL CRASHES

Fuhao Mo,Shuyong Duan,Xiaoqing Jiang,Sen Xiao,Zhi Xiao,Wei Shi,Kai Wei 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.2

Objective: With widely usage of restraint system, fatal injuries to occupants have been largely limited while non-fatal lower extremity injuries have not been effectively improved. The present study aims to investigate occupant lower extremity injuries under realistic impact environments. Methods: A biofidelic lower extremity model, a dummy model and a car cab model were combined to set up a realistic impact environment. Three typical frontal impact groups were simulated. Occupant global lower kinematics, long bone axial force and bending moment were presented to in-depth investigate lower extremity injury mechanism and tolerance. Results: Various overlap frontal impacts cause totally different lower extremity kinematics in the combination of structural invasion and restraint system effects. The femur fracture occurred at a small axial force of 7.57 kN combing a substantial bending moment peak of 172 Nm. Ankle joint injuries were found in 100 % and 25 % overlap impacts that present large tibial axial force and joint rotation angle. Conclusions: Overall results indicate that a coupling threshold of femur axial force and bending moment is of rationality to predict global femur fracture. The ankle joint injury occurrence is significantly related to the coupling effects of tibia axial force and excessive self-kinematics.