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      • KCI등재

        Power line interference noise elimination method based on independent component analysis in wavelet domain for magnetotelluric signal

        Xiaoling Cao,Liangjun Yan 한국자원공학회 2018 Geosystem engineering Vol.21 No.5

        With the urbanization in recent years, the power line interference noise in electromagnetic signal is increasing day by day, and has gradually become an unavoidable component of noises in magnetotelluric signal detection. Therefore, a kind of power line interference noise elimination method based on independent component analysis in wavelet domain for magnetotelluric signal is put forward in this paper. The method first uses wavelet decomposition to change single-channel signal into multi-channel signal, and then takes advantage of blind source separation principle of independent component analysis to eliminate power line interference noise. There is no need to choose the layer number of wavelet decomposition and the wavelet base of wavelet decomposition according to the observed signal. On the treatment effect, it is better than the previous power line interference removal method based on independent component analysis. Through the de-noising processing to actual magnetotelluric measuring data, it is shown that this method makes both the apparent resistivity curve near 50 Hz and the phase curve near 50 Hz become smoother and steadier than before processing, i.e., it effectively eliminates the power line interference noise.

      • KCI등재

        Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3

        Yan, Yutao,Ding, Yu,Ming, Bingxia,Du, Wenjiao,Kong, Xiaoling,Tian, Li,Zheng, Fang,Fang, Min,Tan, Zheng,Gong, Feili Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.5

        Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF-${\alpha}$ or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.

      • KCI등재

        The Production of Bioflocculants by Bacillus licheniformis Using Molasses and Its Application in the Sugarcane Industry

        Xiaoling Zhuang,Yuanpeng Wang,Qingbiao Li,Shan Yan,Ning He 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.5

        A bioflocculant produced by B. licheniformis was investigated with regard to a low-cost culture medium and its industrial application. Molasses replaced sucrose as the sole carbon source in bioflocculant fermentation. The optimum low-cost culture medium was determined to be composed of 20 g/L molasses, 0.4 g/L urea, 0.4 g/L NaCl,0.2 g/L KH2PO4, 1.6 g/L K2HPO4, and 0.2 g/L MgSO4. The bioflocculant from B. licheniformis was then applied to treat sugarcane-neutralizing juice to remove colloids,suspended particles, and coloring matters in a sugar refinery factory. The optimal operation conditions were a bioflocculant dosage of 21 U/mL, pH 7.3 and a heating temperature of 100oC. The color and turbidity of the sugarcane juice reached IU 1267 and IU 206, respectively,after clarification with the bioflocculant; these values were almost the same as those acquired following treatment with polyacrylamide (PAM), the most widely applied flocculant in sugar industries. These results suggest the great potential for use of bioflocculants in the sugar refinery process. A bioflocculant produced by B. licheniformis was investigated with regard to a low-cost culture medium and its industrial application. Molasses replaced sucrose as the sole carbon source in bioflocculant fermentation. The optimum low-cost culture medium was determined to be composed of 20 g/L molasses, 0.4 g/L urea, 0.4 g/L NaCl,0.2 g/L KH2PO4, 1.6 g/L K2HPO4, and 0.2 g/L MgSO4. The bioflocculant from B. licheniformis was then applied to treat sugarcane-neutralizing juice to remove colloids,suspended particles, and coloring matters in a sugar refinery factory. The optimal operation conditions were a bioflocculant dosage of 21 U/mL, pH 7.3 and a heating temperature of 100oC. The color and turbidity of the sugarcane juice reached IU 1267 and IU 206, respectively,after clarification with the bioflocculant; these values were almost the same as those acquired following treatment with polyacrylamide (PAM), the most widely applied flocculant in sugar industries. These results suggest the great potential for use of bioflocculants in the sugar refinery process.

      • KCI등재

        Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3

        Yutao Yan,Yu Ding,Bingxia Ming,Wenjiao Du,Xiaoling Kong,Li Tian,Fang Zheng,Min Fang,Zheng Tan,Feili Gong 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.5

        Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF- or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.

      • KCI등재

        Microstructure, Texture Characteristics, Mechanical and Bio-Corrosion Properties of High Strain Rate Rolled Mg–Zn–Sr Alloys

        Hongge Yan,Xiaole Gong,Jihua Chen,Meixin Cheng 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

        Microstructure, texture characteristics, mechanical and bio-corrosion properties of the Mg–5Zn–xSr alloys (x=0, 0.2, 0.6,1.0) prepared by high strain rate rolling are carefully studied. A low level of Sr addition enhances dynamic precipitationof nano-scale MgZn2 particles in the as-rolled Mg–5Zn alloy, with 0.6%Sr showing the maximum efciency. These highdensity MgZn2 particles can pin grain boundaries of dynamic recrystallization grains and inhibit their growth. A low levelof Sr addition (≤0.6%) enhances the (0002) basal texture, but 1.0%Sr is just the reverse. The as-rolled Mg–5Zn–0.6Sr alloyexhibits the best combination of ultimate tensile strength (359 MPa) and elongation to rupture (20%). The high strength canbe attributable to a reduced grain size, precipitation and basal texture strengthening. The Mg–5Zn–xSr alloys exhibit excellent bio-corrosion resistance, but a minor Sr addition cannot bring about further bio-corrosion resistance improvement dueto the multiple actions of grain size, DRX degree, dynamic precipitates and texture characteristics. The 0.6%Sr addition cangreatly improve the strength of the as-rolled alloy at no expense of bio-corrosion resistance.

      • KCI등재

        Maternal undernutrition alters the skeletal muscle development and methylation of myogenic factors in goat offspring

        Zhou Xiaoling,Yan Qiongxian,Liu Liling,Chen Genyuan,Tang Shaoxun,He Zhixiong,Tan Zhiliang 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6

        Objective: The effects of maternal undernutrition during midgestation on muscle fiber histology, myosin heavy chain (MyHC) expression, methylation modification of myogenic factors, and the mammalian target of rapamycin (mTOR) signaling pathway in the skeletal muscles of prenatal and postnatal goats were examined. Methods: Twenty-four pregnant goats were assigned to a control (100% of the nutrients requirement, n = 12) or a restricted group (60% of the nutrients requirement, n = 12) between 45 and 100 days of gestation. Descendants were harvested at day 100 of gestation and at day 90 after birth to collect the femoris muscle tissue. Results: Maternal undernutrition increased (p<0.05) the fiber area of the vastus muscle in the fetuses and enhanced (p<0.01) the proportions of MyHCI and MyHCIIA fibers in offspring, while the proportion of MyHCIIX fibers was decreased (p<0.01). DNA methylation at the +530 cytosine-guanine dinucleotide (CpG) site of the myogenic factor 5 (MYF5) promoter in restricted fetuses was increased (p<0.05), but the methylation of the MYF5 gene at the +274,280 CpG site and of the myogenic differentiation (MYOD) gene at the +252 CpG site in restricted kids was reduced (p<0.05). mTOR protein signals were downregulated (p<0.05) in the restricted offspring. Conclusion: Maternal undernutrition altered the muscle fiber type in offspring, but its relationship with methylation in the promoter regions of myogenic genes needs to be elucidated. Objective: The effects of maternal undernutrition during midgestation on muscle fiber histology, myosin heavy chain (MyHC) expression, methylation modification of myogenic factors, and the mammalian target of rapamycin (mTOR) signaling pathway in the skeletal muscles of prenatal and postnatal goats were examined.Methods: Twenty-four pregnant goats were assigned to a control (100% of the nutrients requirement, n = 12) or a restricted group (60% of the nutrients requirement, n = 12) between 45 and 100 days of gestation. Descendants were harvested at day 100 of gestation and at day 90 after birth to collect the femoris muscle tissue.Results: Maternal undernutrition increased (p<0.05) the fiber area of the vastus muscle in the fetuses and enhanced (p<0.01) the proportions of MyHCI and MyHCIIA fibers in offspring, while the proportion of MyHCIIX fibers was decreased (p<0.01). DNA methylation at the +530 cytosine-guanine dinucleotide (CpG) site of the myogenic factor 5 (MYF5) promoter in restricted fetuses was increased (p<0.05), but the methylation of the MYF5 gene at the +274,280 CpG site and of the myogenic differentiation (MYOD) gene at the +252 CpG site in restricted kids was reduced (p<0.05). mTOR protein signals were downregulated (p<0.05) in the restricted offspring.Conclusion: Maternal undernutrition altered the muscle fiber type in offspring, but its relationship with methylation in the promoter regions of myogenic genes needs to be elucidated.

      • KCI등재후보
      • KCI등재

        Morphology-Controlled Synthesis and Electrochemical Characteristics of Fe2O3 Nanorods

        Bo Bai,Xiaole Yan,Gang Li,Pengwei Li,Jie Hu,Huabei Jiang,Wendong Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.12

        In this work, highly uniform single crystal Fe2O3 nanorods have been synthesized by a facile hydrothermal method in the presence of dihydrogen phosphate ions. Phosphate ions were speculated capping to the sidewalls of Fe2O3 nanocrystals, and resulted in the anisotropic growth of hematite crystals along their [006] zone axis. Fe2O3 nanorods with various aspect ratios have been realized by applying different phosphate concentration of 0.1–0.4 mM. The electrochemical properties of Fe2O3 nanorods showed that the samples with the smallest aspect ratio possessed superior specific capacitance and stability. It was speculated that the larger specific area of the Fe2O3 nanorods with the shortest axial length facilitated the efficient access of electrolyte ions to the electrode surface, and thus would aid in delivering the high pseudocapacitance. These results provide a promising route to obtain the desired hematite-based energy storage materials.

      • KCI등재후보
      • KCI등재

        Identification and differential expression analysis of anthocyanin biosynthetic genes in root-skin color variants of radish (Raphanus sativus L.)

        Rugang Yu,Xueling Du,Jing Li,Lan Liu,Chaomeng Hu,Xiaoling Yan,Yuqing Xia,Huijuan Xu 한국유전학회 2020 Genes & Genomics Vol.42 No.4

        Background Taproot skin color is a major trait for assessing the commercial and nutritional quality of radish, and red-skinned radish is confirmed to improve consumer’s interest and health. However, little is known about the molecular mechanisms responsible for controlling the formation of red-skinned radish. Objective This study aimed to identify the differentially expressed anthocyanin biosynthetic genes between red- and whiteskinned radishes and understand the molecular regulatory mechanism underlying red-skinned radish formation. Methods Based on the published complete genome sequence of radish, the digital gene expression profiles of Yangzhouyuanbai (YB, white-skinned) and Sading (SD, red-skinned) were analyzed using Illumina sequencing. Results A total of 3666 DEGs were identified in SD compared with YB. Interestingly, 46 genes encoded enzymes related to anthocyanin biosynthesis and 241 genes encoded transcription factors were identified. KEGG pathway analysis showed that the formation of red-skinned radish was mainly controlled by pelargonidin-derived anthocyanin biosynthetic pathway genes. This process included the upregulation of PAL, C4H, 4CL, CHS, CHI, F3H, DFR, LDOX, and UGT enzymes in SD. CHS genes were specifically expressed in SD, and it might be the key point for red pigment accumulation in red-skinned radish. Furthermore, MYB1/2/75, bHLH (TT8), and WD 40 showed higher expression in SD than in YB. Meanwhile, the corresponding low-abundance anthocyanin biosynthesis enzymes and upregulation of MYB4 might be the factors influencing the formation of white-skinned radish. Conclusion These findings provide new insights into the molecular mechanisms and regulatory network of anthocyanin biosynthesis in red-skinned radish.

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