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Xiao‑xia Ma,Yi‑ning Wang,Xiao‑an Cao,Xue‑rui Li,Yong‑sheng Liu,Jian‑hua Zhou,Xue‑peng Cai 한국유전학회 2018 Genes & Genomics Vol.40 No.9
The nucleocapsid (N) protein of peste des petits ruminants virus (PPRV) with a conserved amino acid usage pattern plays an important role in viral replication. The primary objective of this study was to estimate roles of synonymous codon usages of PPRV N gene and tRNA abundances of host in the formation of secondary structure of N protein. The potential effects of synonymous codon usages of N gene and tRNA abundances of host on shaping different folding units (α-helix, β-strand and the coil) in N protein were estimated, based on the information about the modeling secondary structure of PPRV N protein. The synonymous codon usage bias was found in different folding units in PPRV N protein. To better understand the role of translation speed caused by variant tRNA abundances in shaping the specific folding unit in N protein, we modeled the changing trends of tRNA abundance at the transition boundaries from one folding unit to another folding unit (β-strand → coil, coil → β-strand, α-helix → coil, coil → α-helix). The obvious fluctuations of tRNA abundance were identified at the two transition boundaries (β-strand → coil and coil → β-strand) in PPRV N protein. Our findings suggested that viral synonymous codon usage bias and cellular tRNA abundance variation might have potential effects on the formation of secondary structure of PPRV N protein.
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VAULT PROTEIN-TEMPLATED ASSEMBLIES OF NANOPARTICLES
XIAO-YING QI,XIAO HUANG,HAI LI,YUSONG WANG,Yun Xia,MEENUBHARATHI NATARAJAN,JUN WEI,Subbu S. Venkatraman,HUA ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.1
Recombinant vault nanoparticles are used as stable nanoscale platforms for controlled self-assembly of various kinds of nanoparticles into the predefined multidimensional architectures. High-yield and uniform discoidal assemblies templated by vaults are constructed from gold nanospheres and quantum dots, while dimeric assemblies are formed from relatively-large gold nanocubes. The vault-templated approach appears to be mainly mediated by the surface and dimensional properties of nanoparticles while less affected by the chemical composition of nanoparticles, making it a universal strategy for fabrication of nanoassemblies with designed properties for potential applications.
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Xiao-Suo He,Yao-Dong Dai,Xiao-Tao He,Qing-Hua He Korean Nuclear Society 2024 Nuclear Engineering and Technology Vol.56 No.4
Information on isotopic composition and geometric structure is necessary for identifying a true warhead. Nevertheless, such classified information should be protected physically or electronically. With a novel Hash encryption algorithm, this paper presents a Monte Carlo-based design of a neutron activation analysis verification module. The verification module employs a thermal neutron source, a non-uniform mask (physically encrypting information about isotopic composition and geometric structure), a gamma detector array, and a Hash encryption algorithm (for electronic encryption). In the physical field, a non-uniform mask is designed to distort the characteristic gamma rays emitted by the inspected item. Furthermore, as part of the Hash algorithm, a key is introduced to encrypt the data and improve the system resolution through electronic design. In order to quantify the difference between items, Hamming distance is used, which allows data encryption and analysis simultaneously. Simulated inspections of simple objects are used to quantify system performance. It is demonstrated that the method retains superior resolution even with 1% noise level. And the performances of anti-statistical attack and anti-brute force cracking are evaluated and found to be very excellent. The verification method lays a solid foundation for nuclear disarmament verification in the upcoming era.
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Xiao, Yang,Liu, Jun,Huang, Xin-En,Ca, Li-Hua,Ma, Yi-Min,Wei, Wei,Zhang, Rong-Xia,Huang, Xiao-Hong,Chang, Juan,Wu, Yi-Jia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23
Objective: To observe treatment effects and safety of fluvoxamine combined with oxycodone prolonged-release tablets in treating patients with moderate to severe cancer pain. Methods: Patients confirmed pathologically with cancer and complicated with moderate to severe pain, were divided into control and experimental groups. Oxycodone prolonged-release tablets, with or without fluvoxamine, were administrated to all study patients until pain relief. Degree of pain relief, dose of oxycodone prolonged-release tablets, side effects and quality of life were compared before and after treatment. Results: In total, 120 patients were recruited. No statistically significant difference was detected regarding age, gender, types of cancer, KPS between two groups of patients (P>0.05). Baseline pain score of patients with moderate pain in treatment and control group was $4.9{\pm}0.8$ and $5.1{\pm}0.8$, respectively; and decreased to $1.8{\pm}1.1$ and $1.2{\pm}1.1$ after treatment, respectively. Pain intensity was significantly reduced in the treatment group (P=0.028). Average daily consumption of oxycodone prolonged-release tablets was ($54.0{\pm}19.6$) mg and ($44.7{\pm}18.7$) mg respectively, which is lower in treatment grpup than in control group, but the difference was not statistically significant (P=0.065). Baseline pain score of patients with severe pain in treatment and control groups were $8.3{\pm}1.1$ and $8.3{\pm}1.1$, respectively; and pain intensity after treatment decreased to $2.9{\pm}1.0$ and $2.3{\pm}1.0$. Pain intensity was significantly reduced in the treatment group, with statistical significance (P=0.026). Average daily consumption of oxycodone prolonged-release tablets was ($132.0{\pm}42.2$) mg and ($110.7{\pm}33.9$) mg, respectively, which is lower in treatment group than in control group, and the difference was statistically significant (P=0.035). In terms of quality of life, patients in treatment group had better performance status, daily activity, mood, and sleep than that in control group (P < 0.05). Patients in two groups had similar side effects, eg., constipation, nausea/vomiting, lethargy, dizziness, itchy skin, dysuria, and ataxia. Lower incidence of nausea/vomiting, lethargy, was obtained from patients in treatment than in control group, while significant low constipation was observed in treatment than in control group (35.0% vs 49.2%, P=0.026). Conclusion: Fluvoxamine combined with oxycodone prolonged-release tablets could be more effective in treating patients with cancer pain, and could reduce the dosage of oxycodone prolonged-release tablets and thus be associated with lower side effects, and improved quality of life.
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A New Bond-Slip Model of Hybrid Bonded FRP-to-Concrete Joints
Xiao-Hua Gao,Lei Gao,Feng Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.1
The dowel effect in hybrid bonded fiber reinforced plastic (HB-FRP) to concrete joints is difficult to quantify and make the anchoring joint instability. A new HB-FRP joint was built, which eliminates bonding between steel and FRP plates. Based on an empirical model of external bonding FRP (EB-FRP) to concrete joints and the new model of slip at debonding, the new unified bond-slip model of the HB-FRP joints is proposed. The parameters that affect the bond-slip model are analyzed. Pull-off tests are conducted for eight specimens with different numbers of mechanical fasteners, which has verified the proposed model. The load-slip relationship at the loaded end of the FRP plate was well predicted by the partial interaction numerical calculation and further verify the theoretical model.
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Structural and Optical Properties of RF Magnetron Reactively Sputtered Ag2O Film
Xiao-Yong Gao,Jiao-Min Ma,Chao Chen,Meng-Ke Zhao,Jin-Hua Gu,Jing-Xiao Lu 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.60 No.5
A series of <111> oriented Ag2O films was deposited onto glass substrates by using RF magnetron reactive sputtering at different flow ratios (FRs) of oxygen to argon. The Ag2O film deposited at FR = 0.667 was the best <111> oriented film due to dual contributions from both lattice strain and film stress. The films’ transmissivity of over 70% in the near-infrared region indicated that Ag2O films are not suitable for applications as transparent conductive films in the visible region. Ag2O films deposited at FRs from 0.467 to 0.800 had optical band gaps ranging from 3.266 eV to 3.107 eV. The redshift in the films’ absorption edge may be attributed to the decrease in the lattice strain with increasing in FR. A series of <111> oriented Ag2O films was deposited onto glass substrates by using RF magnetron reactive sputtering at different flow ratios (FRs) of oxygen to argon. The Ag2O film deposited at FR = 0.667 was the best <111> oriented film due to dual contributions from both lattice strain and film stress. The films’ transmissivity of over 70% in the near-infrared region indicated that Ag2O films are not suitable for applications as transparent conductive films in the visible region. Ag2O films deposited at FRs from 0.467 to 0.800 had optical band gaps ranging from 3.266 eV to 3.107 eV. The redshift in the films’ absorption edge may be attributed to the decrease in the lattice strain with increasing in FR.
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Identification and evolutionary history of the DD41D transposons in insects
Xiao-Gu Zhang,Hua-Hao Zhang,Yi-Hong Shen,Xiao-Min Xiong,Min-Jin Han 한국유전학회 2016 Genes & Genomics Vol.38 No.2
The rosa monophyletic group of transposons is a group of transposable element with characteristics of encoding a DD41D motif in the catalytic domain. However, biology and evolutionary history of this monophyletic group are still poorly understood. In this study, we report the first description for the presence of a rosa transposon in the silkworm Bombyx mori. Further analyses confirmed that this element in the silkworm genome had recently amplified and might still be capable of transposition. In addition, we present evidence, based on searches of publicly available insect genomes, that a new clade of the rosa monophyletic group was identified. Interestingly, analysis of their three dimensional structures suggested that these proteins showed highly similar protein structures with that of the Mos1 transposase. These results provided useful insights into the functionality of these transposases and their structural and functional deviations from other transposases in the Tc1/mariner superfamily. Meanwhile, sequence and phylogenetic analysis confirmed that DD41D and maT elements might represent another independent large group of the Tc1/mariner superfamily. Importantly, the result of the comparison of terminal inverted repeats (TIRs) validated that DD41D and maT elements almost had identical consensus terminal sequences (50-CAGGGTGNS NCA-30), implying they might have similar cleavage sites or patterns during the process of their transposition. In a word, this study will enrich and expand our knowledge of the Tc1/mariner superfamily.
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