http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Xianyu Piao (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Xianyu Piao,Jung-Woo Kim,Moonjung Hyun,Zhao Wang,Suk-Gyun Park,In A Cho,Je-Hwang Ryu,Bin-Na Lee,Ju Han Song,Jeong-Tae Koh 생화학분자생물학회 2023 BMB Reports Vol.56 No.10
Osteoporosis is a major public health concern, which requiresnovel therapeutic strategies to prevent or mitigate bone loss. Natural compounds have attracted attention as potential therapeuticagents due to their safety and efficacy. In this study, weinvestigated the regulatory activities of boeravinone B (BOB), anatural rotenoid isolated from the medicinal plant Boerhaviadiffusa, on the differentiation of osteoclasts and mesenchymalstem cells (MSCs), the two main cell components responsiblefor bone remodeling. We found that BOB inhibited osteoclastdifferentiation and function, as determined by TRAP stainingand pit formation assay, with no significant cytotoxicity. Furthermore,our results showing that BOB ameliorates ovariectomyinducedbone loss demonstrated that BOB is also effective invivo. BOB exerted its inhibitory effects on osteoclastogenesis bydownregulating the RANKL/RANK signaling pathways, includingNF-κB, MAPK, and PI3K/Akt, resulting in the suppression ofosteoclast-specific gene expression. Further experiments revealedthat, at least phenomenologically, BOB promotes osteoblast differentiationof bone marrow-derived MSCs but inhibits their differentiationinto adipocytes. In conclusion, our study demonstratesthat BOB inhibits osteoclastogenesis and promotes osteoblastogenesisin vitro by regulating various signaling pathways. Thesefindings suggest that BOB has potential value as a novel therapeuticagent for the prevention and treatment of osteoporosis.
-
Mechanisms underlying diabetes-induced bone loss
송주한,Xianyu Piao,고정태 대한구강생물학회 2024 International Journal of Oral Biology Vol.49 No.2
Diabetes, a chronic hyperglycemic condition, is caused by insufficient insulin secretion or functional impairment. Long-term inadequate regulation of blood glucose levels or hyperglycemia can lead to various complications, such as retinopathy, nephropathy, and cardiovascular disease. Recent studies have explored the molecular mechanisms linking diabetes to bone loss and an increased susceptibility to fractures. This study reviews the characteristics and molecular mechanisms of diabetes-induced bone disease. Depending on the type of diabetes, changes in bone tissue vary. The molecular mechanisms responsible for bone loss in diabetes include the accumulation of advanced glycation end products (AGEs), upregulation of inflammatory cytokines, induction of oxidative stress, and deficiencies in insulin/IGF-1. In diabetes, alveolar bone loss results from complex interactions involving oral bacterial infections, host responses, and hyperglycemic stress in periodontal tissues. Therapeutic strategies for diabetes-induced bone loss may include blocking the AGEs signaling pathway, decreasing inflammatory cytokine activity, inhibiting reactive oxygen species generation and activity, and controlling glucose levels; however, further research is warranted.
-
Song Ju Han,김정우,Lee Mi Nam,Oh Sin-Hye,Piao Xianyu,Wang Zhao,권승희,김옥수,고정태 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3
BACKGROUND: The use of mouse bone marrow mesenchymal stem cells (mBMSCs) represents a promising strategy for performing preclinical studies in the field of cell-based regenerative medicine; however, mBMSCs obtained via conventional isolation methods have two drawbacks, i.e., (i) they are heterogeneous due to frequent macrophage contamination, and (ii) they require long-term culturing for expansion. METHODS: In the present study, we report a novel strategy to generate highly pure mBMSCs using liposomal clodronate. This approach is based on the properties of the two cell populations, i.e., BMSCs (to adhere to the plasticware in culture dishes) and macrophages (to phagocytose liposomes). RESULTS: Liposomal clodronate added during the first passage of whole bone marrow culture was selectively engulfed by macrophages in the heterogeneous cell population, resulting in their effective elimination without affecting the MSCs. This method allowed the generation of numerous high-purity Sca-1?CD44?F4/80- mBMSCs ([95%) with just one passaging. Comparative studies with mBMSCs obtained using conventional methods revealed that the mBMSCs obtained in the present study had remarkably improved experimental utilities, as demonstrated by in vitro multilineage differentiation and in vivo ectopic bone formation assays. CONCLUSION: Our newly developed method, which enables the isolation of mBMSCs using simple and convenient protocol, will aid preclinical studies based on the use of MSCs.
ScienceON
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
