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Xiangmin Chen,Guoqiang Shu,Kang Zhang,Meng Duan,Luping Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.3
The fault characteristics of rolling bearing with variable rotational speed are usually related to shafting speed and vary with time. Moreover, the rolling bearing fault characteristics are easily submerged by the noises. To address these issues, an adaptive time-varying comb filtering (ATVCF) method that combines the merits of comb filter and adaptive timevarying filtering (ATVF) is proposed and applied to extract the fault-related component from the envelope signal of rolling bearing. And on this basis, via the joint application of ATVCF and order tracking (OT), a fault characteristics extraction methodology for rolling bearing with variable rotational speed, namely ATVCF-OT, is developed. In the ATVCF-OT, the ATVCF method can adaptively extract time-varying harmonic components containing rolling bearing fault information from the rolling bearing fault vibration signal, and the OT analysis can effectively stabilize the time-varying rolling bearing fault features. Therefore, the ATVCF-OT methodology is particularly suitable for fault feature extraction of rolling bearing with variable rotational speed. Simulation and experimental results indicate that the ATVCF-OT method can effectively remove the fault-unrelated components and highlight the fault features of rolling bearing. The comparisons with the direct envelope order method and the ensemble empirical mode decomposition (EEMD)-based envelope order method demonstrate the advantages of the proposed ATVCF-OT method.
Yan, XiangMin,Wang, Jia,Li, Hongbo,Gao, Liang,Geng, Juan,Ma, Zhen,Liu, Jianming,Zhang, Jinshan,Xie, Penggui,Chen, Lei Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9
Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.
Xuesen Du,Xiangmin Wang,Yanrong Chen,Xiang Gao,Li Zhang 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.36 No.-
Cu and Fe sulfates were loaded on a Ce–Ti mixed oxide and tested for the reduction of NO with NH3. Theloading of metal sulfate greatly enhances the resistance to SO2 of the Ce–Ti oxide. The prepared catalystsalso show better performances after doping with K2O if compared to the V–W–TiOx catalyst. Surfacecharacterizations reveal that better resistance to potassium oxide can be attributed to better acidity andreducibility. The catalyst with iron sulfate supported on Ce–TiOx was found to be a promising catalyst forNO removal in the flue gas with high alkali metal content.
Huiqiang Wu,Xiangmin Wang,Yang Liu,Yu Zhao,Xinyue Wang,Woo-Kyoung Lee,심영기,Il Yoon,Xinming Xu,Jiazhu Li 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.5
In this article, we report the stereoselective Horner?Wadsworth?Emmons reaction of reactive carbonyl-substituted chlorophyll a derivatives with diethyl cyanomethylphosphonate. We found that the formyl or keto group at the chlorin periphery can react stereoselectively with phosphonate carbanions to produce a series of cyanomethylene-substituted chlorins with predominantly E geometry. Considering that no consistent records are available in the literature on the results of the allomerization of methyl pyropheophorbide a, we propose a reliable process for the transformation of this chlorophyll derivative. Our methodology represents a simple and efficient way for the preparation of novel, differently functionalized long-wavelength absorbing chlorins, those can be applied for photodynamic therapy, solar cells, and other relevant purposes.
Efficient Metal-Free Synthesis of Dihydro[1,3]oxazines Assisted by Intramolecular Hydrogen Bonding
Jian Wang,Xiangmin Wang,Zhong Zheng,Jihua Ma,Xu Wang,이우경,심영기,윤일,Jinchun Chen,Jiazhu Li 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.9
A novel and efficient intramolecular hydrogen bonding assisted approach to various dihydro[1,3]oxazines is described. Reaction is carried out under ambient condition without any metal catalysts.
Expression Vectors for Human-mouse Chimeric Antibodies
Xiong, Hua,Ran, Yuliang,Xing, Jinliang,Yang, Xiangmin,Li, Yu,Chen, Zhinan Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.4
The production of recombinant antibodies has been generally recognized as time-consuming and labor-intensive. The aim of our study is to construct mammalian expression vectors containing the cDNA encoding the human constant regions and murine variable regions to massively and cost-effectively produce full-length chimeric antibodies. Unique restriction sites flanking the Ig variable region were designed to allow for the replacement of variable regions generated by PCR. Western blot analysis of the chimeric antibodies revealed that the expressed products were of the predicted size, structure and specificity. The usefulness of the vectors was confirmed by construction of human-mouse chimeric antibody-HCAb which secretes murine antibody against the human colorectal cancer. Selected in medium containing gradually increasing methotrexate (MTX), clones with increased expression of the product gene can be efficiently generated. The secretion of recombinant chimeric antibody-HCAb yielded $30\;pg\;cell^{-1}\;day^{-1}$ at $10^{-6}\;M$ MTX. With this high-level expression from pools, the convenient and rapid production of over 100 milligram amounts per liter of recombinant antibodies may be achieved, which indicates the significant roles of pYR-GCEVH and pYR-GCEVL in the production of chimeric antibodies.
Wenping Hua,Weiwei Kong,XiaoYan Cao,Chen Chen,Qian Liu,Xiangmin Li,Zhezhi Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.5
Dioscorea zingiberensis is the main plant source of diosgenin, a precursor for the production of steroid hormones used in the pharmaceutical industry. The extraction process of diosgenin from D. zingiberensis can generate high-acid and high-strength wastewater on a large scale and can threaten the environment. Bioengineering microorganisms to produce diosgenin is an effective way to avoid pollution. However, little is known about the genes that are involved in the biosynthesis of diosgenin. We obtained 85,010 unigenes (average length of 1142 bases) from the D. zingiberensis transcriptome through RNAseq. A large number of unigenes (59,368; 69.83%) were annotated, and 2488 unigenes were assigned to 27 secondary- metabolite pathways. In our database, 66 unigenes encoding up to 40 key enzymes were found to be present in diosgenin biosynthesis pathways. In addition, we found 203 unigenes encoding CYP450 proteins and 47 unigenes encoding UGT proteins that may be involved in modifications of a downstream pathway. The expression patterns of key diosgenin biosynthesis genes were studied to identify the most important members of the enzyme family. These results add to the available genetic data of D. zingiberensis and lay the foundation for the further production of diosgenin using genetic engineering.