Study on Properties of Contact Lenses Surface-coated with a Monosaccharide-containing Monomer 2-(α-D-Mannopyranosyloxy)ethyl Methacrylate

( Wu Shengnan ),권영환 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0

A contact lens containing silicone polymers is coated with a monosaccharide-containing monomer 2-(α-D-mannopyranosyloxy)ethyl methacrylate (ManEMA) with the help of plasma treatment and the use of SCA to give an increased hydrophilicity. Silicone lenses are first treated with plasma and reacted with SCA. ManEMA is grafted or immobilized at an elevated temperature. Surface grafting typically involves the propagation of the ManEMA to form an anchored chain, wherein a competition between solution and interfacial reactions occurs. Surface crosslinking may also occur. Finally, the extent of surface grating of contact lenses by measuring the thickness using FE-SEM as well as the increased hydrophilicity of contact lenses as a result of the surface grafting are investigated.

1P-16 Synthesis and Characterization of a Monosaccharide-containing Monomer 2-(α-D-Mannopyranosyloxy)Ethyl Methacrylate

( Wu Shengnan ),권영환,( Van Nga Nguyen ) 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

2-(α-D-Mannopyranosyloxy)ethyl methacrylate (ManEMA) was synthesized and isolated as an easy-to-handle dry white solid. It was prepared by reaction of D-mannose with 2-hydroxethyl methacrylate (HEMA) in three steps. To protect 2,3,4,5,6-hydroxy from being reacted, first we need to protect D-mannose with acetic anhydride. Then protected D-mannose with HEMA was conducted in dry acetonitrile using BF3·OEt2 as a Lewis acid. Finally removal of acetyl groups was carefully performed by using methanolic sodium methoxide to give ManEMA. 2-HEMA is a well-know vinyl monomer and it’s corresponding polymer is one of the most successful biologically compatible materials for optical lens and ocular implants. Hence, glycopolymers based on poly(HEMA) are expected to be a good platform for developing diverse biomaterials.

Influence of graphene oxide on the transport and deposition behaviors of colloids in saturated porous media

Peng, Shengnan,Wu, Dan,Ge, Zhi,Tong, Meiping,Kim, Hyunjung Elsevier Applied Science Publishers 2017 Environmental pollution Vol.225 No.-

<P><B>Abstract</B></P> <P>The effects of graphene oxide (GO) on the transport and deposition behaviors of colloids with different sizes in packed quartz sand were investigated in both NaCl (10 and 50 mM) and CaCl<SUB>2</SUB> solutions (1 and 5 mM) at pH 6. Fluorescent carboxylate-modified polystyrene latex microspheres (CMLs) with size ranging from 0.2 to 2 μm were utilized as model colloids. Both breakthrough curves and retained profiles of colloids in the presence and absence of GO in suspensions under all examined solution conditions were analyzed. The breakthrough curves of all three different-sized CMLs with GO were higher yet the retained profiles were lower than those without GO at both examined ionic strengths in NaCl solutions. The observation showed that GO increased the transport and decreased the deposition of all three different-sized CMLs in NaCl solutions. However, in CaCl<SUB>2</SUB> solutions, opposite observation was achieved at two different ionic strength conditions. Specifically, the presence of GO increased the transport and decreased the deposition of all three different-sized CMLs in 1 mM CaCl<SUB>2</SUB> solutions, whereas, it decreased the transport and increased the deposition of all three different-sized CMLs in 5 mM CaCl<SUB>2</SUB> solutions. Comparison the breakthrough curves and retained profiles of CMLs versus those of GO yielded that the overall transport and deposition behaviors of all three different-sized CMLs with GO copresent in suspensions agreed well with the transport and deposition behaviors of GO under all examined conditions. The transport and deposition behaviors of CMLs in packed porous media clearly were controlled by those of GO under the conditions investigated in present study due to the adsorption of CMLs onto GO surfaces. Our study showed that once released into natural environment, GO would adsorb (interact with) different types of colloids and thus have significant influence on the fate and transport of colloids in porous media.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The transport of CMLs in quartz sand was affected by GO present in suspensions. </LI> <LI> GO increased all three sized CMLs transport in quartz sand in NaCl solutions. </LI> <LI> GO had opposite effects on CMLs transport at low and high IS in CaCl<SUB>2</SUB> solutions. </LI> <LI> Adsorption of CMLs onto GO surfaces led to altered transport of CMLs. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The differentiation of rat-induced pluripotent stem cells into alveolar type II epithelial cells with a three-step induction protocol

Bei Wu,Chen Wang,Feilong Hei,Cun Long,Mengmeng Chen,Shengnan Yang,Jie Yu,Zhihai Ju 한국통합생물학회 2015 Animal cells and systems Vol.19 No.1

Induced pluripotent stem (iPS) cells derive from autologous somatic cells, the application prospect of iPS cells forregenerative medicine and tissue engineering is better than embryonic stem cells (ESCs) to some extent. Alveolar type II(AT II) epithelial cells play key role in the injured lung tissue regeneration and function recovery. The differentiation of iPScells into AT II cells could provide available source for injured lung treatment. In this study, rat iPS (riPS) cells wereresuscitated and proliferated for 14 days before differentiation. A modified three-step induction protocol similar to thereported ESCs inducing procedure was used in this study for the differentiation groups. Routine cell culture was done to theriPS cell control group (riPS-con). At stage 3, cells of day 7 (Diff. 7) and day 14 (Diff. 14) were collected for the real-timepolymerase chain reaction tests for gene expressions of Oct4, Nanog, SPA, SPB, SPC, SPD, and CC10. Immunofluorescencestaining of SPC and SSEA-1 was conducted. At the end of the differentiation, cell morphology becameoutstretched and epithelium-like. Cells of the Diff. 14 group positively expressed SPC and negatively expressed SSEA-1,which is contrary to the riPS-con group. In the Diff. 7 and the Diff. 14 groups, the expression of Oct4, Nanog, and SPBdecreased (P < 0.05), whereas the expression of SPA, SPC, SPD (P < 0.05), and CC10 (P > 0.05) increased. This studyindicated that riPS cells can successfully differentiate into AT II epithelial cells with the three-step induction protocol andmay be further applied to implanting in decellularized rat lung scaffolds and building a bio-artificial lung.

Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9

Li Guodong,Li Dan,Wu Chun,Li Shengnan,Chen Feng,Li Peng,Ko Chung-Nga,Wang Wanhe,Lee Simon Ming-Yuen,Lin Ligen,Ma Dik-Lung,Leung Chung-Hang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

In hypoxia and hyperglycemia, SET7/9 plays an important role in controlling HIF-1α methylation and regulating the transcription of HIF-1α target genes, which are responsible for angiogenesis and wound healing. Here, we report the Ir(III) complex Set7_1a bearing acetonitrile (ACN) ligands as a SET7/9 methyltransferase inhibitor and HIF-1α stabilizer. Interestingly, Set7_1a could engage SET7/9 and strongly inhibit SET7/9 activity, especially after preincubation with homocysteine (Hcy), which is elevated in diabetes. We hypothesize that Set7_1a exchanges ACN subunits for Hcy to disrupt the interaction between SET7/9 and SAM/SAH, which are structurally related to Hcy. Inhibition of SET7/9 methyltransferase activity by Set7_1a led to reduced HIF-1α methylation at the lysine 32 residue, causing increased HIF-1α level and recruitment of HIF-1α target genes that promote angiogenesis, such as VEGF, GLUT1, and EPO, in hypoxia and hyperglycemia. Significantly, Set7_1a improved wound healing in a type 2 diabetic mouse model by activating HIF-1α signaling and downstream proangiogenic factors. To our knowledge, this is the first Hcy-targeting iridium compound shown to be a SET7/9 antagonist that can accelerate diabetic wound healing. More importantly, this study opens a therapeutic avenue for the treatment of diabetic wounds by the inhibition of SET7/9 lysine methyltransferase activity.

A Fuzzy Cerebellar Model Articulation Controller Based Visual Servo System for Robot

Wei Sun,Cong Wang,Shengnan Liu,Baoqiang Wu,Minghua Ouyang 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.2

This paper presents a novel online learning visual servo controller integrating the FCMAC with proportion controller for the control of position of manipulator end-effector. Since the FCMAC has good learning capability and fast learning speed, and can save much computer memory space by fuzzy processing of input space division and memory unit activation, it is used to develop an adaptive control law by learning the relationship between the image feature errors and manipulator input, and the aim of online learning of the FCMAC is to minimize the output of proportion controller. Further-more, the FCMAC has no need for models of robot manipulator and image feature extraction, so that the capability of proposed controller for tasks under uncertain environment can be improved. Finally, the proposed controller is proved to be effective by the experiment, and compared with BP neural net-work.

Compound heterozygous mutations of NDUFV1 identified in a child with mitochondrial complex I deficiency

Tang Xiaojun,Xu Wuhen,Song Xiaozhen,Ye Haiyun,Ren Xiang,Yang Yongchen,Zhang Hong,Wu Shengnan,Lan Xiaoping 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Mitochondrial complex I deficiency (MCID) is the most common biochemical defect identified in childhood with mitochondrial diseases, mainly including Leigh syndrome, encephalopathy, macrocephaly with progressive leukodystrophy, hypertrophic cardiomyopathy and myopathy. Objective: To identify genetic cause in a patient with early onset autosomal recessive MCID. Methods: Trio whole-exome sequencing was performed and phenotype-related data analyses were conducted. All candidate mutations were confirmed by Sanger sequencing. Results: Here we report a child of Leigh syndrome presented with global developmental delay, progressive muscular hypotonia and myocardial damage. A missense mutation c.118C > T (p.Arg40Trp) and a previously reported mutation c.1157G > A (p.Arg386His) in NDUFV1 have been identified as compound heterozygous in the patient. The mutation p.Arg386His is closely associated with the impairment of 4Fe-4S domain and this mutation has been reported pathogenic. The c.118C > T mutation has not been reported in ClinVar and HGMD database. In silico protein analyses showed that p.Arg40 is highly conserved in a wide range of species, and the amino acid substitution p.Trp40 largely decreases the stability of NDUFV1. In addition, the mutation has not been detected in the Asian populations and it was predicted to be deleterious by numerous prediction tools. Conclusion: This research expands the mutation spectrum of NDUFV1 and substantially provides an early and accurate diagnosis basis of MCID, which would benefit subsequently effective genetic counseling and prenatal diagnosis for future reproduction of the family.