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The Studies on In Vivo Embryo Production using Sex-Sorted Sperm in Hanwoo
Yeoung-Gyu Ko,Sung Woo Kim,Dong-Kyo Kim,Nam-Tae Kim,Chan-Lan Kim,Ik-Soo Jeon,Min-Su Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each 12.58 ± 8.31 and 13.25 ± 7.86. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen (3.75 ± 1.98 vs. 8.23 ± 6.07, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.
전준수(Junsu Jeon),이정호(Jungho Lee),우성필(Seongphil Woo),임지혁(Ji-Hyuk Im),이광진(Kwang-Jin Lee),유병일(Byungil Yoo),조남경(Nam-Kyung Cho),한영민(Yeoung-Min Han) 한국추진공학회 2016 한국추진공학회 학술대회논문집 Vol.2016 No.12
개발 중에 있는 9톤급 다단연소방식 엔진은 크게 예연소기, 터보펌프, 연소기로 구성되어 있다. 이 중 예연소기와 연소기는 시험을 위한 점화원을 필요로 하며, 점화제로는 TEA/B 혼합액을 사용하고 있다. 점화제를 안정적으로 공급하기 위하여 점화제 용기인 TEA amplue을 설계 및 제작하였다. 제작된 TEA ampule은 강도/기밀 시험을 통하여 안정성을 확보하였으며, 향후 예연소기 및 연소기의 점화에 사용될 예정이다. A staged combustion cycle engine consists of the pre-burner, the turbo-pump and the combustion chamber. The pre-burner and the combustion chamber are needed an ignition source for a test. An ignition agent is used the TEA/B mixture liquid. A TEA ampule was designed and manufactured for stable supplying of an ignition agent. The stability of manufactured TEA ampule was confirmed by strength and leakage test. And it will be used for an ignition of the pre-burner and the combustion test.
임지혁(Ji-Hyuk Im),우성필(Seongphil Woo),전준수(Junsu Jeon),이정호(Jungho Lee),이광진(Kwang-Jin Lee),한영민(Yeoung-Min Han) 한국추진공학회 2017 한국추진공학회 학술대회논문집 Vol.2017 No.5
정지궤도용 우주발사체에는 고성능 상단엔진이 필수적이며 높은 비추력을 가지는 다단연소사이클 엔진이 적합하다. 터보펌프, 예연소기, 연소기, 공급계 시스템으로 구성된 9톤급 다단연소사이클 엔진 시스템의 기술검증시제를 제작하여 나로우주센터 3단 엔진 연소시험설비에서 3초 지상연소시험을 수행하였다. 엔진 시스템의 시동, 점화, 연소 및 종료가 정상적으로 수행되었으며 주요 성능 변수를 평가하였다. High performance upper stage engine is necessary for space launch vehicles of geostationary orbit, and staged combustion cycle engine is suitable due to high specific impulse. Technology demonstration model for 9 tonf class staged combustion cycle engine, which is consisted of turbopump, preburner, combustion chamber and supply system, was assembled, and hot-firing test was conducted for three seconds in Upper-stage Engine Test Facility of Naro Space Center. Ignition, combustion and shut down of engine system was performed normally, and its performance parameters were evaluated.
MS 마커를 활용한 지역별 오계 유전자원의 다양성 및 유연관계 분석
노희종(Hee-Jong Roh),김관우(Kwan-Woo Kim),이진욱(Jin-Wook Lee),전다연(Da-Yeon Jeon),김승창(Seung-Chang Kim),전익수(Ik-Soo Jeon),고응규(Yeoung-Gyu Ko),이준헌(Jun-Heon Lee),김성희(Sung-Hee Kim),백준종(Jun-Jong Baek),오동엽(Dong-Yep Oh) 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.3
본 연구는 연산오계(천연기념물 제265호)와 이를 기원으로 하는 5개 지역별 오계 집단의 유전적 특성 및 차별성을 분석하기 위해 25개의 초위성체(MS) 마커를 이용하여 총 9개 집단 243수를 대상으로 유전자형을 분석하였다. 마커별 다형성 분석 결과, 총 153개의 대립유전자가 확인되었으며, Hexp와 PIC의 경우 MCW0145에서 각각 0.640, 0.570으로 가장 높았고, Hobs는 MCW0252에서 0.607로 가장 높은 값을 나타내었다. 반면, LEI0166에서 Hexp, Hobs, PIC가 각각 0.248, 0.204, 0.202로 가장 낮았다. 집단간 유전거리 분석 결과로는 9개 집단중 YSO 집단과 SUO 집단이 가장 가까운(0.073) 반면, LG 집단과 CBO 집단 사이에서 가장 먼(0.937) 것으로 확인되었다. 집단의 실제 구조를 확인하기 위한 집단별 균일도를 분석한 결과, 공시된 9개의 집단은 3개의 집단으로 구분했을 때 최적의 K값(7.96)을 얻을 수 있었으며, 5개의 오계 집단(YSO, ARO, CBO, CNO, SUO) 및 LG 집단과 CN․RIR 집단은 각각 1, 2, 3번 군집에 분포하고 있는 것으로 나타났다. 한편, GBO 집단의 경우 1번과 3번 클러스터에 걸쳐서 분포하고 있는 것으로 보아 사육과정에서 타집단과의 교잡이 일어났을 것으로 추정된다. 이러한 결과를 통해 추후 오계 유전자원에 대한 국가 수준의 유전적 특성평가 및 관리의 기초 자료로 유용하게 활용될 것으로 기대된다. The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (ΔK=7.96). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.
Al-SiCp 복합재료에서 SiCp 의 용해거동에 관한 연구
김석원,이의권,전우용 ( Sug Won Kim,Eui Kweon Lee,Woo Yeoung Jeon ) 한국주조공학회 1993 한국주조공학회지 Vol.13 No.4
N/A Aluminum base composites reinforced with various amount of SiC particles and Mg contents have been investigated by different fabrication method for twenty-years. In this paper, how the decomposition and dissolution behaviors of SiCp(20㎛) in the melt of Al composites arised was studied. As the results, the decomposition and dissolution of SiCp into the melt of Al composites increased with increase of the temperature above 720℃, and holding time at a given melting temperature. Because SiC is thermodynamically unstable in this Al-SiCp composite at temperature above the liquidus, SiCp dissolves and reacts with Al in matrix to form Al₄C₃according to following chemical equation 4Al+3SiC→Al₄C₃+3Si, Si decomposed and dissolved from SiCp increases Si content of matrix, while liquidus temperature of matrix decrease with increase of SiC content in matrix. The hardness of SiCp decreased with increase of the melting temperature, the hardness of the matrix /particle interface increased with increase of the melting temperature due to increase of the Mg₂Si and Al₄C₃intermetallic compounds, etc.
Al-Si / SiCp 복합조직에 미치는 Rheo-compocasting 의 제조조건 및 Mg 첨가의 영향
김석원,이의권,전우용 ( Sug Won Kim,Eui Kweon Lee,Woo Yeoung Jeon ) 한국주조공학회 1993 한국주조공학회지 Vol.13 No.6
N/A Dispersion behaviors of SiC particles and microstructures in Al-2%Si/SiCp composite prepared by Rheo-compocasting were studied with change of fabrication conditions(slurry temperature, agitation time) and additions of Mg(0∼3wt.%). Also, the microhardness change of matrix, interface and total in composites were examined with additions of Mg (0∼3wt.%). The dispersion of particles in the composites became relatively homogeneous with increase of Mg additions, agitation time and decrease of slurry temperature. Rate of occupied area by particle in matrix was increased as increase of Mg additions due to improvement of wettability between SiC particle and matrix. A favorable composites were obtained by melting under Ar atmospheric SiCp injection and bottom pouring system. According to the analysis of X-ray diffraction, Mg₂Si, Al₄C₃, SiO₂and MgO, etc, intermetallic compounds were formed by chemical interreaction at interface of matrix and particles. The microhardness of interface is higher than that of matrix due to more strengthening of above intermetallic compounds. It was considered that the total hardness of the composites is improved by dispersing of SiCp and addition of Mg.