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- 저자 : 이광진(Kwang-Jin Lee) (검색결과 467 건)
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On-line Screening HPLC-ABTS<sup>+</sup> Assay을 이용한 산청목으로부터 Salidroside의 분리 및 생물활성 분석
이광진,송나영,마진열,Lee, Kwang Jin,Song, Na-Young,Ma, Jin Yeul 한국생물공학회 2014 KSBB Journal Vol.29 No.2
Acer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as on-line screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase fromAcer tegmentosum was a traditional Korean herbal medicine showing various pharmacological activities. In this work, A. tegmentosum were extracted with boiling water and then successively partitioned with dichloromethane, ethyl acetate, n-butyl alcohol (n-BuOH), and water. Salidoside, the target compound, was purified in n-BuOH phase using a chromatography method. For the analysis of salidoside, TLC and LC-MS were used as well as online screening $HPLC-ABTS^+$ assay with three different wavelength of 254, 280, and 320 nm. An amount of 1.34 g of salidoside were obtained in n-BuOH phase from 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum. 3 kg of dry biomass. The on-line screening $HPLC-ABTS^+$ assay is rapid and efficient tool to search bioactivity from A. tegmentosum.
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A6061-T6/AZ31 이종금속 마찰교반접합부의 기계적 특성에 미치는 툴 삽입 위치의 영향
이광진,김상혁,Lee, Kwang-Jin,Kim, Sang-Hyuk 대한용접접합학회 2012 대한용접·접합학회지 Vol.30 No.5
Butt-joints of A6061-T6 and AZ31 plates were successfully manufactured by using a friction stir welding method. Off-set free joints and off-set joints were fabricated to investigate the effect of the tool plunge position on the mechanical properties of the joints. Hardness test, tensile test and charpy impact test was performed to evaluate the mechanical properties of the joints. Off-set condition resulted in increase of mechanical properties of the joints. The variation of the hardness distribution in the SZ was also stabilized in the off-set condition. Tensile strength of the off-set joint was about 85% against to that of the AZ31 base metal. Impact absorption energy by Charpy-Impact-Test of the off-set joint also increased by almost 2 times against to that of the AZ31 base metal.
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마찰교반용접(FSW) 및 마찰교반처리(FSP)의 최신 연구개발 동향
이광진,Lee, Kwang-Jin 대한용접접합학회 2013 대한용접·접합학회지 Vol.31 No.2
The latest research & development trend on friction stir welding and friction stir processing technologies presented in the international symposium, 'Friction Stir Welding & Processing VII'. Papers and presentations about high temperature materials such as advanced high strength steel, stainless steel and titanum alloy shoot up this year. Papers on modeling of metal flow and control of process parameters also increased. The FSP technologies for manufacturing of carbon materials reinforced metal matrix composites were reported, too.
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이광진,김형자,Kwang-Jin, Lee,Hyung-Ja, Kim 한국기계가공학회 2004 한국기계가공학회지 Vol.3 No.3
The sub-surface stress field beneath the spur gear's contact surface in lubricated condition has been analysed. The surface pressure was obtained by the elasto-hydrodynamic lubrication analysis using the accurate geometric clearances around the contact region of the teeth. The sub-surface stress field was calculated by using the Love's rectangular patch solution. The analysis results show that the sub-surface stress distribution is quite dependent on the surface pressure distribution. The pattern of sub-surface stress field is similar to that of the external load. The depth where the maximum effective stress occurs is not proportional to the intensity of the external load.
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이광진,김상혁,권의표,손규송,Lee, Kwang-Jin,Kim, Sang-Hyuk,Kwon, Eui-Pyo,Son, Kyu-Song 대한용접접합학회 2014 대한용접·접합학회지 Vol.32 No.2
Friction stir welding (FSW) was successfully performed about 900MPa grade Twinning Induced Plastisity (TWIP) steel. A PCBN tool with convex-type shoulder was applied. Optimal process conditions were deduced. Microstructure and mechanical properties such as hardness, tensile strength and impact absorbed energy were observed and evaluated, respectively. An optical microscope (OM) and a field emission scanning electron microscope (FE-SEM) was selected for observing the grain structure.
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Al-Mg-Si계 알루미늄 합금 판재 마찰교반접합부의 결정 방위 분포에 대한 용접후열처리의 영향
이광진,Lee, Kwang-Jin 대한용접접합학회 2009 대한용접·접합학회지 Vol.27 No.6
Friction stir welding (FSW) was carried out for Al-Mg-Si series aluminum alloys which are being used for automotive body structure. Consequently, Post weld heat treatment (PWHT) was applied to the friction stir welds to evaluate the effect of the paint baking process which is one of the automotive fabrication process on friction stir welded zone (FSWZ) in 443K for 1.2Ks. Grain structure and its crystal orientation distribution was measured about both the as welded specimens and the post weld heat treated specimens. An optical microscope (OM) and an field emission scanning electron microscope (FE-SEM) was used for observing the grain structure and measuring its crystal orientation distribution, respectively. Changes on the grain structure and its crystal orientation distribution were not detected. From the present results, it was confirmed that the paint baking process after FSW do not affect on the grain structure and its crystal orientation distribution of FSWZ. The comprehensive investigations will be performed for various automotive aluminum alloys manufactured by different processes, in the future.
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분사기 배열과 추진제 유량 변화에 의한 축소형 연소기의 연소특성
이광진(Kwang-Jin Lee),서성현(Seonghyeon Seo),김성구(Seong-Gu Kim),한영민(Yeoung-Min Han),최환석(Hwan-Seok Choi) 한국추진공학회 2008 한국추진공학회 학술대회논문집 Vol.2008 No.5
추진제 유량 변화와 분사기 배열에 따른 특성속도를 연구하기 위해 축소형 연소기를 이용하여 연소시험을 수행하였다. 그 결과 유사한 압력과 혼합비 조건에서 효과적인 비 단위 면적당 유량 범위가 존재함을 알 수 있었고 최외곽 분사기로부터 막 냉각이 적용된 연소실 벽면까지의 거리 증가는 연소실 내 저 혼합비 영역을 증가시키고 특성속도를 저감시키는 기능을 하는 것으로 해석결과 나타났다. 즉, 이 2가지 설계요소는 주어진 연소압력에서 액체로켓엔진 연소기의 성능을 향상시킬 수 있는 중요한 인자임을 확인하였다. Hot firing tests of sub-scale combustors were carried out to study the characteristic velocity according to the variation of propellant mass flow and injector arrangement. Test results show that there exists an effective range of relative flow-rate density on the condition of similar combustion pressure and mixture ratio. Numerical analysis has also revealed that the increase of the distance between the outermost injector array and the cylindrical chamber wall with film cooling increases the region of low mixture ratio near combustion chamber wall and it decreases the characteristic velocity of the combustor. Thus, it was confirmed that these two factors play an important part in improving the performance of LRE combustor on a predetermined chamber pressure.
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