Synthesis of Triglyceride of Conjugated Linoleic Acid (CLA) by Lipozyme

Won-Seck Park,Seck-Jong Kim,Kyung-Ah Park,Jeong-Ok Kim,Eun-Joo Lee,Dong-Gil Lim,Yeong-Lae Ha 한국식품영양과학회 2000 Preventive Nutrition and Food Science Vol.5 No.2

Most fatty acids in food matrices are triglyceride (TG) forms. Conjugated linoleic acid (CLA) produced from linoleic acid by microorganisms or chemicals is a free form. To apply the CLA to food systems, the TG containing CLA (designate CLA-TG) was synthesized by Lipozyme-catalyzed esterification method. An optimum reaction condition for the esterification of free CLA (FCLA) to glycerol by Lipozyme was determined as follows; Lipozyme (50 mg) effectively catalyzed the esterification of CLA (500 mg) to glycerol (1150 mg) dissolved in isooctane (3 ml) in a shaking incubator (200 rpm, 50℃) for 48 hr. Under the reaction condition, the resultant contained 52.4% CLATG as well as 31.1% Di-CLA-glycerol (CLA-DG), 7.6% mono-CLA-glycerol (CLA-MG), and 9.0% other CLA (unreacted FCLA plus CLA dimer). These results suggest that the Lipozyme could be a useful enzyme for the production of CLA-TG to be employed in foods.

Synthesis of Triglyceride of Conjugated Linoleic Acid(CLA) by Lipozyme

Park, Won-Seck,Kim, Seck-Jong,Park, Kyung-Ah,Kim, Jeong-Ok,Lee, Eun-Joo,Lim, Dong-Gil,Ha, Yeong-Lae The Korean Society of Food Science and Nutrition 2000 Preventive Nutrition and Food Science Vol.5 No.2

Most fatty acids in food matrices are triglyceride(TG) forms. Conjugated linoleic acid(CLA) produced from linoleic acid by microorganisms or chemicals is a free form. To apply the CLA to food systems, the TG containing CLA(designate CLA-TG) was synthesized by Lipozyme-catalyzed esterification method. An optimum reaction con-dition for the esterification of free CLA(FCLA) to glycerol by Lipozyme was determined as follows; Lipozyme(50mg) effectively catalyzed the esterification of CLA(500mg) to glycerol(1150mg) dissolved in isooctane (3ml) in a shaking incubator (200 rpm, 5$0^{\circ}C$) for 48 hr. Under the reaction condition, the resultant contained 52.4% CLA-TG as well as 31.1% Di-CLA-glycerol(CLA-DG), 7.6% mono-CLA-glycerol(CLA-MG), and 9.0% other CLA(un-reacted FCLA plus CLA dimer). These results suggest that the Lipozyme could be a useful enzyme for the production of CLA-TG to be employed in foods.

Conjugated Linoleic Acid (CLA) Glycerol 유도체의 화학적 합성

박원석(Won-Seck Park),김석종(Seck-Jong Kim),박숙자(Sook-Jahr Park),김정옥(Jeong-Ok Kim),임동길(Dong-Gil Lim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.3

CLA의 glycerol 유도체를 화학적으로 합성하였다. CLA-Cl(1.79 mmole), glycerol(0.6 mmole)과 pyridine(1.3 mL)를 25C에서 8시간 반응시켰다. 이 반응물을 SGCC와 TLC를 이용하여 CLA의 glycerol 유도체를 분리하고, ¹H-NMR, ¹³C-NMR, IR, MS를 이용하여 분리된 유도체를 동정하였다. 이 실험조건하에서 사용된 CLA의 59.4%가 CLA의 glycerol 유도체(CLA-TG, CLA-DG, CLAMG)로 전환되었다. CLA의 glycerol 유도체 중 CLA-TG는 52.1%, CLA-DG는 17.0%, CLA-MG는 30.9%였다. Conjugated linoleic a cid (CLA) is a potent anticarcinogen for several animal models. CLA was synthesized by alkaline isomerization of linoleic acid. Derivatives of CLA with glycerol were synthesized by chemical methods to use as food additives. Chemically-synthesized CLA-chloride (CLA-Cl, 1.79 mmole), glycerol (0.6 mmole) and pyridine (1.3 ml) were reacted at 25℃ for 8 hrs. The resultant was fractionated by silica gel column chromatography (SGCC) and thin layer chromatography (TLC). The fractions were identified using infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (NMR), and mass spectrometry (MS). Amount of CLA converted to CLA-glycerol derivatives was 59.4% and the rest of CLA was remained as unreacted CLA or CLA dimer. The composition of the CLA-glycerol derivatives was 52.1% tri-CLA- glycerol (CLA-TG), 17.0% di-CLA-glycerol (CLA-DG) and 30.9% mono-CLA-glycerol (CLA-MG). These results suggest that the chemical synthesis of CLA-glycerol derivatives produces CLA-TG, CLA- DG and CLA-MG as well as CLA dimer.

소비자 유형에 따른 막걸리 소비 행태 분석 -광주광역시 주류 소비자를 중심으로-

서정원 ( Jeong Won Seo ),조규대 ( Gue Dae Cho ),김인석 ( In Seck Kim ) 한국식품유통학회 2015 食品流通硏究 Vol.32 No.2

The Makgeolli industry had expanded rapidly since the late 2008 in response to significant increase of exports as well as various government supports. However, the expansion of Makgeolli industry has slowed down in recent years due to the various reasons. It is essential for Makgeolli industry to correctly examine consumer purchasing behavior in order to restore its increasing market share in the alcohol market. This study classified Makgeolli consumers based on the patterns of lifestyle and analyzed purchasing behavior using survey data. Empirical results showed that the perceptions, places to drink, choice attributes of Makgeolli are statistically significantly different depending on the lifestyle of consumers. The findings of this study would provide empirical basis to develop proper market segmentation strategies in the Makgeolli industry to expand its market.

뽕나무가루 첨가 배지에서 배양한 버섯균사체 배양물의 자유라디칼 유도 산화 억제

김석종(Seck-Jong Kim),임동길(Dong-Kil Lim),박철우(Cherl-Woo Park),세르보로다메(Rhoda Mae Cerbo),형석원(Seok-Won Hyung),이강권(Kang-Kweon Lee),김정옥(Jeong-Ok Kim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2

뽕나무가루 첨가 배지에서 배양한 느타리버섯균사체 배양물의 조추출물이 자유라다칼로 유도한 linloeic acid의 산화를 대조구에 비해 75.9% 감소시켰고, mouse liver microsome 산화에서도 NADPH/Fe^(++) system에서 64.3% 감소시켰다. 이와 같은 효과는 조추출물로부터 용매분획한 분획물의 단독 효과보다 우수하였다. 상황 및 동충하초버섯균사체 배양물의 조추출물도 느타리버섯균사체 배양물의 조추출물과 효과가 유사하였지만 다소 낮았다. Antioxidant activity of extracts from the submerged-liquid culture of mushrooms was measured using two systems: linoleic acid and mouse liver microsomes induced by various free radical sources. Mushrooms of Pleurotus ostreatus (Neutari), Phellinus linteus (Sanghwang), Paecilomyces japonicus (Dongchunghacho), Hericicum erinacium (Norugungdengyee) and Agaricus blazei (Shinryeong) in 1% mulberry tree powder-supplemented medium were incubated in a shaking incubator (200 rpm, 25℃) for 3 days. Hot water extracts of mycelial cultures were freeze-dried, followed by fractioning with hexane, chloroform, ethylacetate, and butanol in the order. Antioxidant activity of each sample was examined in free radical-induced linoleic acid oxidation in phosphate-buffered saline (PBS) solution by measuring the amount of malonaldehyde (MA), and mouse liver microsomal systems by measuring the amount of thiobarbituric acid reactive substances (TBARS). In linoleic acid oxidation system, hot water extracts from the cultures of Pleurotus ostreatus, Phellinus linteus, and Paecilomyces japonicus exhibited stronger antioxidant activity than aqueous or butanol fraction and the combined fraction of hexane, chloroform and ethylacetate, but the hot water extract from Pleurotus ostreatus culture was the strongest activity. The antioxidant activity of the hot water extract from Pleurotus ostreatus culture was stronger than any other fractions in mouse microsomal system. These results suggest that hot water extract of Pleurotus ostreatus culture, and the cultures of Phellinus linteus and Paecilomyces japonicus could be useful for functional materials to reduce the oxidation of lipids in food systems induced by free radicals.

곡형 콘크리트 충진 강관기둥 부재의 횡방향 저항성능 평가

정영수(Jeong Young Soo),박무수(Park Moo Su),최형석(Choi Hyung Seck),안진희(Ahn Jin Hee),이원홍(Lee Won Hong) 한국방재학회 2017 한국방재학회논문집 Vol.17 No.2

콘크리트 충진 강관부재들은 교량의 교각이나 건축물의 기둥으로 사용되고 있다. 콘크리트 충진 강관부재를 다양한 구조물에 효율적으로 적용하기 위해서는 구조물에 작용하는 하중위치 거동 특성 등을 고려하여야 구조적 장점을 충분히 활용할 수 있다. 절개지 및 사면에 사용되고 있는 낙석방지 울타리의 주기둥은 수평방향으로 작용하게 되는 외력에 저항하여야 하는 안전시설이다. 이러한 낙석방지 울타리의 주기둥을 콘크리트 충진 곡형강관으로 적용하여 하중에 저항하는 단면성능을 확인하고 작용하중에 대한 하중저항성능을 확인하기 위하여 강관 두께, 충진 콘크리트 강도 및 충진높이 변화와 작용방향 및 하중높이 등을 변화하여 콘크리트 충진강관 기둥부재의 하중저항 성능 변화를 구조해석프로그램을 이용하여 비교하였다. 또한 기존 낙석방지 주기둥의 성능도 함께 비교하여 성능변화를 비교하였다. Concrete-Filled tube structures(CFT) have been widely installed in structural system as the bridge pier and column of buildings. The CFT member on various structure has been effectively utilized to consider the structural behavior and loading condition. The rockfall protection fence was installed to prevent rockfall from cut slopes and resisted by lateral loading. In this study, FE analyses were conducted to examine the sectional performance and load-resistant performance of curved concrete filled circular steel column by applied lateral load. FE model was considered in thickness of curvature circular steel column, compressive strength of concrete, filled height of concrete, loading direction and loading location. Additionally, their structural performances were compared to those of an installed rockfall protection fence as H-beam.

뽕나무 첨가 배지에서 배양한 버섯균사체 배양물의 자동산화 억제 효과

김석종(Seck-Jong Kim),임동길(Dong-Kil Lim),형석원(Seok-Won Hyung),김미숙(Mee-Sook Kim),김정옥(Jeong-Ok Kim),김무남(Mu-Nam Kim),이강권(Kang-Kweon Lee),하영래(Yeong-Lae Ha) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2

BMM에 여러 가지 버섯균을 7일 동안 액체배양하고 균사를 제거한 배양물에 대한 항산화성에 관한 연구를 수행하였다. 시료(1 ㎎)를 275 μmol linoleic acid가 함유된 배양액에 첨가하고 shaking incubator(200 rpm, 40℃)에 16일 동안 배양하면서 생성된 POV와 MA의 함량의 조사하였다. 이 결과를 BM에서 배양한 버섯균사체배양물과 BMM 자체의 POV 및 MA 생성 결과와 비교하였다. AB-BMM과 HE-BMM의 항산화능이 다른 버섯균사체배양물이나 BMM보다 우수하였다. 따라서 이 연구결과는 뽕나무는 버섯균사체액체배양물의 항산화성을 증가시켰고, AB-BMM 및 HE-BMM은 항산화물로 활용할 수 있음을 의미한다. Effect of mulberry tree powders on the antioxidant activity of submerged-liquid culture of mushrooms was investigated. Agaricus blazei (AB), Hericicum erinacium (HE), Pleurotus ostreatus (PO), Phellinus linteus (PL) and Paecilomyces japonicus (PJ) were cultured in a shaking incubator (200 rpm, 25℃) for 7 days in culture media: 1) basal medium (BM) and 2) BM+1% mulberry tree powders (BMM). Hot water extracts from the submerged-liquid cultures of mushrooms and BMM were freeze-dried for the measurement of antioxidant activity, of which reaction mixture (25 mL: 10 mL of 0.2 M sodium phosphate buffer, pH 8.0; 4.5 mL distilled water; and 10.5 mL ethanol) contained 275 μmol linoleic acid and one mg test sample. The reaction mixture was incubated in a shaking incubator (200 rpm, 40℃) for 16 days. Peroxide value (POV) was measured for a period of over 16 days, and malonaldehyde (MA) was determined only for samples from the day 16 of incubation. Mycelial weight of mushroom strains cultured in BMM was greater than BM. The antioxidant activities of AB-cultured in BMM (AB-BMM) and HE-cultured in BMM (HE-BMM) were superior to those of other mushroom strains-cultured in BMM or BM and of BMM. These results suggest that mulberry tree powders enhance the antioxidant activity of submerged-liquid culture of mushroom strains. The AB-BMM and HE-BMM were the most active cultures.

Selenium이 MPTP(1-methy-4-phenyl-1,2,3,6-tetrahydropyridine)에 의해 유도된 생쥐의 신경독성에 미치는 영향

김석환,이주연,김여정,강혜옥,이항우,최종원,Kim Seck-Hwan,Lee Joo-Yeon,Kim Yeo-Jeong,Kang Hye-Ok,Lee Hang-Woo,Choi Jong-Won 한국생명과학회 2006 생명과학회지 Vol.16 No.2

MPTP에 의해 유도된 Parkinsonism에 대한 selenium의 보호효과와 그 보호작용에 대한 항산화적 해독기전을 조사하기 위하여 MPTP 10mg/kg을 6일간 주사하고 selenium (25, 50, 100 ${\mu}g/kg$)을 10일간 경구 투여하였으며 처음 6일간은 selenium와 MPTP를 병용 투여하였다. 실험동물을 마지막으로 selenium을 투여하고 24시간 후에 치사시켜 일반적인 독성과 항산화 방어능과 관련된 지표성분과 monoamine oxidase와 같은 신경생화학적인 지표성분들을 뇌조직에서 측정하였으며 그 결과 다음과 같은 결론을 얻었다. 우선 MPTP를 투여함에 따라 운동능력이 저하되던 것이 selenium을 투여함에 따라 운동능력이 증가되었으며, 이러한 결과의 기전은 selelnium을 투여함으로써 MPTP를 $MPP^+$로 대사시키는 MAO-B의 활성을 억제하였으며 $MPP^+$에 의해 유도된 신경독성에 대한 selenium의 보호 효과는 selenium을 투여함으로써 활성산소 해독계인 SOD, catalase, glutathione peroxidase의 활성을 증가시키기 때문인 것으로 사료된다. This study is investigated the effect of selenium against neurotoxicity induced by MPTP(1-methy-4-phenyl-propion-oxypiperidine) in mice. In order to demonstrate neuroprotective activity of selenium, mice were administrated orally with selenium(25, 50, 100 ${\mu}g/kg$, once/day) for 10 days, and MPTP(10 mg/kg) was injected subcutaneously into the mice for 6 days from the beginning 1hr before selenium treatment. Test of rota road activity was inhibited by treatment with selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. Monoamine oxidase(MAO)-B activity and cerebral lipid peroxide content were significantly decreased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice and MAO-A was not affected. Activities of cerebral superoxide dismutase, catalase and glutathione peroxidase were significantly increased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. These results suggest that selenium might be estimated the result from the cooperative action of its inhibitory effect on monoamine oxidase-B with that of the enhancement of antioxidant(SOD, catalase, GSH-Px) defence ability.

HPLC-PDA-ESI-MS/MS identification and quantification of phenolic acids in enriched Isatidis Folium extract and assessment of its anti-wrinkle activity

Gao Dan,Chong Woon Cho,Rustam Rustamov,Cheong Tae Kim,Won Seck Jeong,Jong Seong Kang 한국분석과학회 2021 학술대회논문집 Vol.2021 No.5

Purification and Amino Acid Sequence of the Linoleate Isomerase Produced from Butyrivibrio fibrisolvens A - 38

Sook-Jahr Park,Kyung-Ah Park,Cherl-Woo Park,Won-Seck Park,Jeong-Ok Kim,Yeong-Lae Ha 한국식품영양과학회 1996 Preventive Nutrition and Food Science Vol.1 No.2

Molecular weight and partial amino acid sequence of the cis,9-cis,12-octadecadienoate isomerase(linoleate isomerase) of Butyrivibrio fibrisolvens A-38 were determined. Linoleate isomerase was isolated from the bacteria cultured anaerobically and purified by ultracentrifugation in conjunction with Sepharose 6B column chromatography, Phenyl sepharose 4B column chromatography and fast performance liquid chromatography(FPLC). The isomerase was a single polypeptide with 19KD of molecular weight, when determined by SDS-PAGE. Fourteen amino acids sequence of N-terminal of the linoleate isomerase was N-GEIDKYPRRIIKQQ determined by Edman method.