Participation of GATA-3 in regulation of bone healing through transcriptional upregulation of bcl-xL expression

Mei-Hsiu Liao,Pei-I Lin,Wei-Pin Ho,Wing P Chan,Ta-Liang Chen,Ruei-Ming Chen 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

We have previously demonstrated the expression of GATA-DNA-binding protein (GATA)-3, a transcription factor, in osteoblasts and have verified its function in transducing cell survival signaling. This translational study was further designed to evaluate the roles of GATA-3 in regulating bone healing and to explore its possible mechanisms. A metaphyseal bone defect was created in the left femurs of male ICR mice. Analysis by micro-computed topography showed that the bone volume, trabecular bone number and trabecular thickness were augmented and that the trabecular pattern factor decreased. Interestingly, immunohistological analyses showed specific expression of GATA-3 in the defect area. In addition, colocalized expression of GATA-3 and alkaline phosphatase was observed at the wound site. As the fracture healed, the amounts of phosphorylated and non-phosphorylated GATA-3 concurrently increased. Separately, GATA-3 mRNA was induced during bone healing, and, levels of Runx2 mRNA and protein were also increased. The results of confocal microscopy and co-immunoprecipitation showed an association between nuclear GATA-3 and Runx2 in the area of insult. In parallel with fracture healing, Bcl-XL mRNA was significantly triggered. A bioinformatic search revealed the existence of a GATA-3-specific DNA-binding element in the promoter region of the bcl-xL gene. Analysis by chromatin immunoprecipitation assays further demonstrated transactivation activity by which GATA-3 regulated bcl-xL gene expression. Therefore, this study shows that GATA-3 participates in the healing of bone fractures via regulating bcl-xL gene expression, owing to its association with Runx2. In the clinic, GATA-3 may be used as a biomarker for diagnoses/prognoses or as a therapeutic target for bone diseases, such as bone fractures.

Controlling the Valence State of Cu Dopant in α-Fe₂O₃ Anodes: Effects on Crystal Structure and the Conversion Reactions with Alkali Ions

Zhang, Jiliang,Lau, Vincent Wing-hei,Liao, Chang-Zhong,Wong, Kam Wa,Lee, Gi-Hyeok,Zou, Feng,Chang, Chung-Kai,Sheu, Hwo-Shuenn,Kang, Yong-Mook American Chemical Society 2019 Chemistry of materials Vol.31 No.4

<P>Doping is one of the most important ways to tailor the performance of energy materials. However, the crystal structure of doped materials is usually oversimplified as a simple substitution of dopants. Here, we characterized the doped α-Fe<SUB>2</SUB>O<SUB>3</SUB> with different Cu cations using synchrotron X-ray diffraction, X-ray absorption, and X-ray photoelectron spectroscopy, and electrochemically evaluated it as an anode in lithium batteries. The results suggest that doping is not the simple replacement of Fe<SUP>3+</SUP> sites by Cu<SUP>2+</SUP> or Cu<SUP>+</SUP> but induces a complex local structure change, which may be a characteristic of this class of materials. In Cu<SUP>+</SUP>-doped samples, Cu<SUP>+</SUP> not only replaces the Fe<SUP>3+</SUP> site and distorts the FeO<SUB>6</SUB> octahedra, but also gives rise to oxygen vacancies in CuO<SUB>6</SUB> octahedra in the bulk structure and peroxides at the surface, leading to uniform precipitation of Cu as a conductive and buffering agent. These CuO<SUB>6</SUB> octahedra also facilitate homogeneous reactions (electrochemical reduction of Cu<SUP>+</SUP> and Fe<SUP>3+</SUP> together) and the formation of high quality solid-electrolyte interface (SEI) layers. All these factors account for its improved electrochemical performance (discharge capacity of 841(25) mAh/g against 758(21) mAh/g of undoped one, after 80 cycles at 100mA/g). In Cu<SUP>2+</SUP>-doped samples, Cu<SUP>2+</SUP> takes both Fe<SUP>3+</SUP> and empty octahedral interstitial sites, forming linear clusters of three neighboring CuO<SUB>6</SUB> octahedra. Such medium-range phase separation causes electrochemical reduction to metallic Cu before the reduction of Fe<SUP>3+</SUP>, leading to inactive surface Cu that contributes to poor SEI layers and deteriorates its electrochemical performances. The present work allows a better understanding of how doping affects the crystallographic structures and offers insights into how this strategy can be employed to improve electrochemical performance, in contrast to the ambiguity over material properties associated with the commonly accepted model of simple atomic replacement.</P> [FIG OMISSION]</BR>

Modifi ed Radiology-Guided Percutaneous Gastrostomy (MRPG) for Patients with Complete Obstruction of the Upper Digestive Tract and Who are without Endoscopic or Nasogastric Access

Siu-Cheung Chan,Winnie Chiu-Wing Chu,Kar-Wai Liu,Chun-Ta Liao,Tsung-Shih Lee,Shu-Hang Ng 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.2

Objective: We wanted to report on our experience with modified radiology-guided percutaneous gastrostomy (MRPG) without endoscopic or nasogastric access for treating patients with complete obstruction of the upper digestive tract. Materials and Methods: Fourteen oncology patients (13 had hypopharyngeal cancer and 1 had upper esophageal cancer) with complete obstruction of the upper digestive tract were recruited. Conventional percutaneous endoscopic gastrostomy (PEG) and radiologic (fl uoroscopy-guided) percutaneous gastrostomy (RPG) were not feasible in all the patients. An MRPG technique (with a combination of ultrasound, an air enema and fl uoroscopic guidance) was performed in these patients. Results: We achieved successfully percutaneous gastrostomy using the modifi ed technique in all patients without any major or minor complications after the procedure. Conclusion: A modifi ed radiology-guided percutaneous gastrostomy technique can be safely performed in patients who failed to receive conventional PEG or RPG due to the absence of nasogastric access in the completely obstructed upper digestive tract. Objective: We wanted to report on our experience with modified radiology-guided percutaneous gastrostomy (MRPG) without endoscopic or nasogastric access for treating patients with complete obstruction of the upper digestive tract. Materials and Methods: Fourteen oncology patients (13 had hypopharyngeal cancer and 1 had upper esophageal cancer) with complete obstruction of the upper digestive tract were recruited. Conventional percutaneous endoscopic gastrostomy (PEG) and radiologic (fl uoroscopy-guided) percutaneous gastrostomy (RPG) were not feasible in all the patients. An MRPG technique (with a combination of ultrasound, an air enema and fl uoroscopic guidance) was performed in these patients. Results: We achieved successfully percutaneous gastrostomy using the modifi ed technique in all patients without any major or minor complications after the procedure. Conclusion: A modifi ed radiology-guided percutaneous gastrostomy technique can be safely performed in patients who failed to receive conventional PEG or RPG due to the absence of nasogastric access in the completely obstructed upper digestive tract.

Antipsychotic Medication in Schizophrenic Patients is Associated with Higher Risks of Developing Bone Fractures and Refractures

Ching-Min Kuo,Wei-Jen Liao,Chun-Che Huang,Tsuo-Hung Lan,Ching-Heng Lin,Shun-Ping Wang,Cheng-Hung Lee,Ping-Wing Lui 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.4

Objective: The relationship of antipsychotics and the risk of refracture in treated patients is unclear. The aim of this study is to evaluate the association between prolonged antipsychotic and the incidences of bone fractures and refractures in schizophrenia. Methods: This is a retrospective nested case-control study using Taiwan National Health Insurance Research Database recorded from 2000 to 2005, with cases followed up to end of 2011. Total of 7,842 schizophrenic patients, 3,955 had developed bone fractures were compared with 3,887 control subjects matched in age, sex, and index date. Antipsychotic drug exposure was classified based on the drug type and medication duration. Conditional logistic regression analyses were performed. Odds ratio (OR) and confidence interval (CI) were calculated. Results: We found (after adjustments) higher risks of developing fractures under continued use of typical (OR = 1.70; 95% CI, 1.51−1.91) or atypical antipsychotics (OR = 1.43; 95% CI, 1.28−1.60) were found. Additionally, continued use typical (OR = 1.84; 95% CI, 1.35−2.50) or atypical antipsychotics (OR = 1.44; 95% CI, 1.06−1.95) was positively associated with refracture risks. Moreover, refractures were associated with continuous use of chlorpromazine (one typical antipsychotics, OR = 2.45; 95% CI, 1.14−5.25), and risperidone (OR = 1.48; 95% CI, 1.01−2.16) or zotepine (OR = 2.15; 95% CI, 1.06−4.36) (two atypical antipsychotics). Conclusion: Higher risks of bone fracture and refracture were found in schizophrenia under prolonged medication with typical or atypical antipsychotics. We therefore recommend that clinicians should pay more attention on bone density monitoring for patients using long-term antipsychotics.

ADSORPTION OF CADMIUM BY FLY ASH FROM HSINTA POWER PLANT

( Jue Fue Sun,Ying Hsien Yang,Suen Zone Lee,Wing P . Liao ) 한국물환경학회 1997 제6차 국제수질보전학회 아시아 태평양지역 학술회의 Vol.2 No.-