A biodegradable drug‐controlled delivery system based on mesoporous manganese dioxide and poly(dopamine)

Li Miaomiao,Cai Wenrong,Jiang Lihua,Li Junyao,Li Shan,Tang Tongtong,Kong Yong 대한화학회 2023 Bulletin of the Korean Chemical Society Vol.44 No.12

Mesoporous manganese dioxide (mMnO 2 ) was first synthesized for the loading of methotrexate (MTX), and then dopamine was in situ polymerized on the surface of the MTX‐loaded mMnO 2 (mMnO 2 ‐MTX) in an alkaline solution to encapsulate the drug in the mesopores of mMnO 2 . Both low pH and glutathione (GSH) can result in the degradation of mMnO 2 and poly(dopamine) (PDA), and thus the delivery of MTX from the mMnO 2 ‐MTX‐PDA can be triggered by low pH and GSH. Near‐infrared (NIR) light‐responsive delivery of MTX can be achieved owing to the outstanding photothermal conversion capability of PDA; on the other hand, the mMnO 2 ‐MTX‐PDA can be utilized for photothermal therapy under the irradiation of NIR light due to the elevated temperature. The results of cytotoxicity test demonstrate that the pH, GSH, and NIR light tri‐responsive drug‐controlled delivery system has excellent biocompatibility, while exhibits pronounced growth inhibition against murine breast tumor cell line 4T1. Mesoporous manganese dioxide (mMnO2) was first synthesized for the loading of methotrexate (MTX), and then dopamine was in situ polymerized on the surface of the MTX-loaded mMnO2 (mMnO2-MTX) in an alkaline solution to encapsulate the drug in the mesopores of mMnO2. Both low pH and glutathione (GSH) can result in the degradation of mMnO2 and poly(dopamine) (PDA), and thus the delivery of MTX from the mMnO2-MTX-PDA can be triggered by low pH and GSH. Near-infrared (NIR) light-responsive delivery of MTX can be achieved owing to the outstanding photothermal conversion capability of PDA; on the other hand, the mMnO2-MTX-PDA can be utilized for photothermal therapy under the irradiation of NIR light due to the elevated temperature. The results of cytotoxicity test demonstrate that the pH, GSH, and NIR light triresponsive drug-controlled delivery system has excellent biocompatibility, while exhibits pronounced growth inhibition against murine breast tumor cell line 4T1.

Cell Division Cycle 2 Protects Neonatal Rats Against Hyperoxia-Induced Bronchopulmonary Dysplasia

Zhongying Li,Yanhong Chen,Wenrong Li,Fan Yan 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.8

Purpose: Hyperoxia-induced bronchopulmonary dysplasia (BPD) is a lung disease in preterm infants. We aimed to explore therole of cell division cycle 2 (CDC2) on histopathologic changes of lung tissues, as well as the viability, apoptosis, and inflammationof lung cells in rats with hyperoxia-induced BPD. Materials and Methods: Hyperoxia-induced BPD in neonatal rats and hyperoxia-induced A549 cells were constructed. The mRNAexpression of CDC2 was detected by qRT-PCR. The fibrosis score of lung tissues was evaluated by hematoxylin-eosin staining. Theviability and apoptosis of A549 cells were detected by cell counting kit-8 assay and flow cytometry. The protein expressions of bcl-2,bax, and caspase-3 were measured by western blot. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β inA549 cells were detected by enzyme-linked immunosorbent assay. The pcDNA3.1-CDC2 was injected into rats to determine therole of CDC2 in hyperoxia-induced BPD in vivo. Results: The expression of CDC2 was decreased in lung tissues of neonatal rats with hyperoxia-induced BPD and hyperoxia-inducedA549 cells. The fibrosis score was increased in the lung tissues of neonatal rats with hyperoxia-induced BPD. Overexpressionof CDC2 increased the viability and protein expression of bcl-2; and inhibited the apoptosis, inflammation, and protein expressionof bax and caspase-3 in hyperoxia-induced A549 cells. Up-regulation of CDC2 alleviated the histopathologic changes in lung tissuesof neonatal rats with hyperoxia-induced BPD. Conclusion: Overexpression of CDC2 promoted the viability and inhibited the apoptosis and inflammation of hyperoxia-inducedcells, and alleviated the histopathologic changes of lung tissues in neonatal rats with hyperoxia-induced BPD.

DISCUSSION ON THE ANALYTIC SOLUTIONS OF THE SECOND-ORDER ITERATED DIFFERENTIAL EQUATION

Liu, HanZe,Li, WenRong Korean Mathematical Society 2006 대한수학회보 Vol.43 No.4

This paper is concerned with a second-order iterated differential equation of the form $c_0x'(Z)+c_1x'(z)+c_2x(z)=x(az+bx(z))+h(z)$ with the distinctive feature that the argument of the unknown function depends on the state. By constructing a convergent power series solution of an auxiliary equation, analytic solutions of the original equation are obtained.

Hyaluronic acid encapsulated aminated mesoporous silica nanoparticles for pH-responsive delivery of methotrexate and release kinetics

Rui Qian,Yin Zheng‐Zhi,Cai Wenrong,Li Junyao,Wu Datong,Kong Yong 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.5

A simple drug controlled delivery system is facilely designed for pH-responsive delivery of methotrexate (MTX), an anticancer drug. Aminated mesoporous silica nanoparticles (AMSNs) were first synthesized for the loading of MTX, and then the MTX-loaded AMSN (AMSN-MTX) was encapsulated with hyaluronic acid (HA) through electrostatic attractions. Successful preparation of the HAencapsulated AMSN-MTX (AMSN-MTX-HA) is confirmed by different characterizations such as scanning electron microscopy, Fourier transform infrared, x-ray diffraction, and so on. Because the protonation/deprotonation of HA is closely related to pH, the electrostatic interactions between HA and AMSN depend closely on the pH of the medium and thus pH-responsive delivery of MTX is achieved. The release kinetic data of MTX from the carrier fit well to Higuchi and Korsmeyer-Peppas models. Cell experiments indicate that the developed AMSN-MTX-HA displays high inhibitory effect on hepatoma (SMMC-7721) cells while the drug-free carrier of AMSN-HA has good biocompatibility.

Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos

Zhang, Xuemei,Wang, Liqin,Wu, Yangsheng,Li, Wenrong,An, Jing,Zhang, Fuchun,Liu, Mingjun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10

Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.

Estimates of linkage disequilibrium and effective population sizes in Chinese Merino (Xinjiang type) sheep by genome-wide SNPs

Shudong Liu,Sangang He,Lei Chen,Wenrong Li,Jiang Di,Mingjun Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.7

Knowledge of linkage disequilibrium (LD) is important for effective genome-wide association studies and accurate genomic prediction. Chinese Merino (Xinjiang type) is well-known fine wool sheep breed. However, the extent of LD across the genome remains unexplored. In this study, we calculated autosomal LD based on genomewide SNPs of 635 Chinese Merino (Xinjiang type) sheep by Illumina Ovine SNP50 BeadChip. A moderate level of LD (r2 ≥ 0.25) across the whole genome was observed at short distances of 0–10 kb. Further, the ancestral effective population size (Ne) was analyzed by extent of LD and found that Ne increased with the increase of generations and declined rapidly within the most recent 50 generations, which is consistent with the history of Chinese Merino sheep breeding, initiated in 1971. We also noted that even when the effective population size was estimated across different single chromosomes, Ne only ranged from 140.36 to 183.33 at five generations in the past, exhibiting a rapid decrease compared with that at ten generations in the past. These results indicated that the genetic diversity in Chinese Merino sheep recently decreased and proper protective measures should be taken to maintain the diversity. Our datasets provided essential genetic information to track molecular variations which potentially contribute to phenotypic variation in Chinese Merino sheep.