Genome-wide Association Studies for Osteoporosis: A 2013 Update

Hong-Wen Deng,Lei Zhang,Christopher J. Papasian,Hong-Wen Deng 대한골대사학회 2014 대한골대사학회지 Vol.21 No.2

In the past few years, the bone field has witnessed great advances in genome-wide as-sociation studies (GWASs) of osteoporosis, with a number of promising genes identified. In particular, meta-analysis of GWASs, aimed at increasing the power of studies by com-bining the results from different study populations, have led to the identification of nov-el associations that would not otherwise have been identified in individual GWASs. Re-cently, the first whole genome sequencing study for osteoporosis and fractures waspublished, reporting a novel rare nonsense mutation. This review summarizes the im-portant and representative findings published by December 2013. Comments are madeon the notable findings and representative studies for their potential influence and im-plications on our present understanding of the genetics of osteoporosis. Potential limi-tations of GWASs and their meta-analyses are evaluated, with an emphasis on under-standing the reasons for inconsistent results between different studies and clarificationof misinterpretation of GWAS meta-analysis results. Implications and challenges ofGWAS are also discussed, including the need for multi- and inter-disciplinary studies.

Novel HMW glutenin genes from Aegilops tauschii and their unique structures

Wen-Jie Chen,Zhong-Wei Yuan,Lian-Quan Zhang,Xing Fan,Ze-Hong Yan,Ji-Rui Wang,You-Liang Zheng,Huai-Gang Zhang,Deng-Cai Liu 한국유전학회 2012 Genes & Genomics Vol.34 No.3

A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx5.3t and 1Dy12.1**t were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3t was between those of 1Bx6 and 1Bx7, while 1Dy12.1**t with slightly faster migration rate than that of 1Dy12. The lengths of 1Dx5.3t and 1Dy12.1**t were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues,respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. 1Dx5.3t is the shortest of the known Dx-type alleles. 1Dy12.1**t is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that 1Dy12.1**t may be a key site in the genealogy of the Glu-Dy.

MiR-214 inhibits apoptosis in thyroid epithelial follicular cells induced by amiodarone through the FASL/MAPK pathway

Wen Jing,Deng Chaonan,Shi Lixin,Zhou Shi,Zhang Miao,Hu Xiaoli,Wang Nianxue,Luo Lijuan 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.2

Background Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is one of the most common autoimmune disease (AITD) in clinical practice. It is urgent to explore the mechanism of amiodarone-induced thyroid dysfunction. Objective This study aims to assess the expression levels of miR-214 and FasL in amiodarone contact type of HT, and the effect of miR-214 on cell viability and apoptosis and potential mechanism. Results We found that miR-214 was low expressed in the tissues of amiodarone-treated thyroiditis patients. MiR-214 increased the survival rate of amiodarone-induced thyroid epithelial follicular cells and inhibited apoptosis. Mechanically, we found that miR-214 could bind to FASL and regulate MAPK signaling pathway through FASL. Conclusions Our results suggested that miR-214 could be a potential therapeutic target for Hashimoto's thyroiditis.

Improving Bricks (kaolin) Performance by adding Incinerator Bottom Ash and Adjusting Kiln Calcination Temperatures

( Deng-fong Lin ),( Huan-lin Luo ),( Wei-zhu Wang ),( Jia-wen Chen ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-

The bottom ash (BA) is the residuals of the municipal trashes after incinerated in an incinerator plant. The BA is usually collected and landfilled as the final disposal treatment. However, it becomes harder finding locations for Taiwan as an island with a very scarce land for the landfill treatment. Moreover, the amount of the BA recycled was far behind the amount produced in which leading to a hard problem for central and local governments. How to properly recycle or reuse of the BA becomes an urgent need. From the results obtained by many different researchers, the use of the BA as a ceramic material may help improve the strength of related products. In this study, the BA was applied as part of the replacement for kaolin in the manufacture of the brick specimens. To investigate the effects of the kiln temperature on the brick specimens and evaluate the related properties of the specimens, the kiln temperatures were set at 850, 900, and 950℃. The municipal solid waste incineration BA was applied as part of kaolin replacement in the manufacture of bricks. Test results showed that the shrinkage increment of bricks tended to be smooth when the BA replacement was larger than 10%. Moreover, the results of loss on ignition indicated that the organic and inorganic matters and heavy metal were mostly burned off at the kiln temperature of 900℃ or above. The compressive strengths of bricks increased with the increase amount of BA replacement. The study of the micro structure observations showed that the silicification of the brick specimen bodies reduced with the increase amount of the BA replacement. Moreover, the results obtained from XRD indicated that the peak intensities of the SiO₂ in the brick specimens decreased with increase amount of the BA replacement. This study suggested that the optimal amount of BA replacement and the kiln temperature were 20% and 950℃, respectively.

Evaluating Service System Alternatives via a Computer Simulation-enabled MCDM Framework

Deng, Wei-Jaw,Pei, Wen,Tsai, Chih-Hung The Korean Society for Quality Management 2007 The Asian Journal on Quality Vol.8 No.2

Decision makers in the service industry must effectively cope with queuing problems, service capacity optimization, service efficiency and service quality problems. This study proposes a computer simulation-enabled MCDM framework that integrates computer simulation analysis, Taguchi method, expert opinion and multiple criteria decision making (MCDM) to assist decision makers in coping with decision problems. In this framework, Taguchi method is adopted to reduce the time required for the simulation experiment. Computer simulation analysis is adopted to obtain useful information for rapid decision-making without interrupting actual production. MCDM is used to select the optimal alternative. The illustrative result is extremely promising.

Concept and Analysis of Stay Cables with a CFRP and Steel Composite Section

Wen Xiong,C.S. Cai,Rucheng Xiao,Lu Deng 대한토목학회 2012 KSCE Journal of Civil Engineering Vol.16 No.1

This paper describes the concept of a novel stay cable with a Carbon Fiber Reinforced Plastic (CFRP) and steel composite section. In this concept CFRP core conserves all the advantages of CFRP materials such as light weight and high strength, while steel coat provides protection for the CFRP core and ensures reliable anchorage performance. The steel coat can reduce cost and provide higher stiffness than CFRP materials in some length range. Following this concept, several configurations were proposed for the composite structure. Effects of the key design parameter of the proposed stay cables, namely the ratio of the CFRP section area to the whole section area, were examined through a parametric study using analytical solutions. By doing this, an appropriate range of the ρ value was suggested. Factors that can affect the appropriate range of the ρ value, including the horizontal projected length of stay cables,cable force, and pylon height (i.e., vertically projected length of stay cables), were also studied. Finally, the feasibility of the proposed stay cables was conceptually verified. It was shown that the proposed stay cables could be an excellent alternative to the pure CFRP or traditional steel stay cables.

Electrochemical Immunosensor for Carcinoembryonic Antigen Detection Based on Mo-Mn3O4/MWCNTs/Chits Nanocomposite Modified ITO Electrode

Wen-Ting Li,Yu Wang,Fen-Fang Deng,Li-Li Liu,Hai-Jun Nan,He Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8

A novel electrochemical immunosensor for determination of carcinoembryonic antigen (CEA) in human serum was fabricated by depositing Mo–Mn3O4/MWCNTs/Chits nanocomposite onto an indium-tin oxide (ITO) electrode. Mo-doped Mn3O4 (MMO) was synthesized by sol–gel method and the presence of molybdenum improved its electrochemical properties. The MMO/ MWCNTs/Chits nanocomposite could accelerate the electron transfer rate and enlarge the surface area to capture a large number of Carcinoembryonic Antigen (CEA). The factors influencing the performance of the immunosensor were investigated, such as incubation time, incubation temperature and pH. Under optimal conditions, the electrochemical immunosensor could detect CEA in a linear range from 0.1 ng · mL-1 to 125 ng · mL-1 with a detection limit of 4.9 pg · mL-1 (S/N = 3). In addition, it exhibited high sensitivity and acceptable stability on a promising immobilization platform for signal amplification, which could be extended to other labeled recognition systems. This electrochemical immunosensor may provide potential applications for the clinical diagnosis.

Clinical Observation and Therapeutic Evaluation of Rh-endostatin Combined with DP Regimen in Treating Patients with Advanced Esophageal Cancer

Deng, Wen-Ying,Song, Tao,Li, Ning,Luo, Su-Xia,Li, Xiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Objective: To observe the curative effects of rh-endostatin combined with DP regimen in treating patients with advanced esophageal cancer and analyze the correlation of CT perfusion (CTP) parameters and the expression of vascular endothelial growth factor (VEGF). Methods: Twenty patients with esophageal cancer confirmed pathologically were randomly divided into combined treatment (rh-endostatin+DP regimen) group and single chemotherapy group, 10 patients in each group, respectively. All patients were given conventional CT examination and CTP imaging for primary tumor. The level of VEGF, the size of tumor and CTP parameters (BF, BV, PS and MTT) before treatment and after 2 cycles of treatment were determined for the comparison and the correlation between CTP parameters and VEGF expression was analyzed. Results: the therapeutic effect of rh-endostatin+DP regimen group was superior to single chemotherapy group. VEGF level after treatment in rh-endostatin+DP regimen group was obviously lower than single chemotherapy group (P<0.01). The expression of VEGF had positive correlation with BF and BV but negative correlation with MTT. Compared with treatment before for rh-endostatin+DP regimen group, BF, BV and PS decreased while MTT increased after treatment (P<0.05). However, there were no significant differences between treatment before and after treatment in single chemotherapy (P>0.05). Conclusions: Rh-endostatin can down-regulate the expression of VEGF in esophageal cancer, change the state of hypertransfusion and high permeability of tumor vessels and had the better curative effect and slighter adverse reactions when combined with chemotherapy.

Behavioral Responses of Pregnant Women to the Early Stage of COVID-19 Pandemic in the Network Era in China: Online Questionnaire Study

Wen-sheng Hu,Sha Lu,Meng-yan Xu,Min-cong Zhou,Zhen-ming Yuan,Yue-yue Deng 한국간호과학회 2021 Asian Nursing Research Vol.15 No.3

Purpose: The aim of this study was to examine the behavioral responses of pregnant women during the early stage of Coronavirus Disease 2019 (COVID-19) outbreak. Methods: We recruited 1,099 women to complete an online questionnaire survey from February 10 to February 25, 2020. The subjects were divided into two groups (the pregnant women group and the control group). Results: Concerns about infection: most of the participants watched the COVID-19 news at least once a day. Protective behaviors: the utilization rate of pregnant women (often using various measures) was higher than that of nonpregnant women. Exercise: 30.6% of the pregnant women continued to exercise at home, whereas in the control group, this percentage was 8.4%. Spouse relationship: 38.8% of the subjects’ relationship improved, whereas only 2.3% thought the relationship was getting worse. Conclusion: Pregnant women had some unique behavioral responses different from that of nonpregnant women. It is important to understand the behavioral responses of pregnant women in this network era.

Study on Chinese Consumers’ Purchase Intention on Cosmetics PPL in Korean(Hallyu) Drama

Deng Xiao-Wen,Soon-Hong Kim 한국유통과학회 2015 KODISA ICBE (International Conference on Business Vol.2015 No.-

In terms of an implication of this study, it was confirmed that hallyu celebrity-oriented imitative psychology, South Korean cosmetics brand image, PPL attitude and others have effects on purchase intention on South Korean cosmetic products and in particular, of the path coefficients, this PPL attitude variable was proved to be the most influential factor and after all, PPL strategies of South Korea’s dramas were verified as a marketing strategy that would work effectively on Chinese consumers. South Korea needs to keep creating cultures and dramas of good quality, and they also must come up with strategies to promote their creations to China.