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- 저자 : Wenjie Wu (검색결과 20 건)
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WenJie Wu,YanXuan Fan,Yan Zhang,QiaoDi Jia,Jie Cao 사단법인 한국질량분석학회 2015 Mass spectrometry letters Vol.6 No.1
This study demonstrated the stabilizing role of a cyclodextrin on Keggin [PW12O40]3- via hydrogen bonding complexationunveiled by ESI-MS. The distinctive fragmentation pathways of the {PW12}/γ-CD complexes from that of discrete[PW12O40]3- showed that the so-called “weak” non-covalent interactions can effectively change the dissociation chemistry ofPOM in the gas phase. The influence of different types of solvents and organic additives such as γ-CD on the stability of Keggin[PW12O40]3- was also addressed firstly by ESI-MS.
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You-Duo Wu,Chuang Xue,Li-Jie Chen,WenJie Yuan,Feng-Wu Bai 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1
Micronutrient zinc is of great importance for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum. The effect of zinc supplementation on toxic metabolites (formic, acetic, butyric acid and butanol) tolerance during ABE fermentation was investigated under various stress-shock conditions without pH control. Great improvements on cell growth, glucose utilization and butanol production were achieved. In the presence of 0.45 g/L formic acid, zinc contributed to 11.28 g/L butanol produced from 55.24 g/L glucose compared to only 5.27 g/L butanol from 29.49 g/L glucose in the control without zinc supplementation. More importantly, relatively higher levels of 7.5 g/L acetic acid, 5.5 g/L butyric acid and 18 g/L butanol could be tolerated by C. acetobutylicum with zinc supplementation while no fermentation was observed under the same stress-shock condition respectively, suggesting that the acids and butanol tolerance in C. acetobutylicum could be significantly facilitated by pleiotropic regulation of micronutrient zinc. Thus, this paper provides an efficient bioprocess engineering strategy for improving stress tolerance in Clostridium species.
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Differential cubature method for buckling analysis of arbitrary quadrilateral thick plates
Wu, Lanhe,Feng, Wenjie Techno-Press 2003 Structural Engineering and Mechanics, An Int'l Jou Vol.16 No.3
In this paper, a novel numerical solution technique, the differential cubature method is employed to study the buckling problems of thick plates with arbitrary quadrilateral planforms and non-uniform boundary constraints based on the first order shear deformation theory. By using this method, the governing differential equations at each discrete point are transformed into sets of linear homogeneous algebraic equations. Boundary conditions are implemented through discrete grid points by constraining displacements, bending moments and rotations of the plate. Detailed formulation and implementation of this method are presented. The buckling parameters are calculated through solving a standard eigenvalue problem by subspace iterative method. Convergence and comparison studies are carried out to verify the reliability and accuracy of the numerical solutions. The applicability, efficiency, and simplicity of the present method are demonstrated through solving several sample plate buckling problems with various mixed boundary constraints. It is shown that the differential cubature method yields comparable numerical solutions with 2.77-times less degrees of freedom than the differential quadrature element method and 2-times less degrees of freedom than the energy method. Due to the lack of published solutions for buckling of thick rectangular plates with mixed edge conditions, the present solutions may serve as benchmark values for further studies in the future.
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Wu, Lanhe,Jiang, Zhiqing,Feng, Wenjie Techno-Press 2004 Structural Engineering and Mechanics, An Int'l Jou Vol.17 No.5
This paper presents a theoretic model of a smart structure, a transversely isotropic piezoelectric thick square plate constructed with three laminas, piezoelectric-elastic-piezoelectric layer, by adopting the first order shear deformation plate theory and piezoelectric theory. This model assumes that the transverse displacements through thickness are linear, and the in-plane displacements in the mid-plane of the plate are not taken to be account. By using Fourier's series expansion, an exact Navier typed analytical solution for deflection and electric potential of the simply supported smart plate is obtained. The electric boundary conditions are being grounded along four vertical edges. The external voltage and non-external voltage applied on the surfaces of piezoelectric layers are all considered. The convergence of the present approach is carefully studied. Comparison studies are also made for verifying the accuracy and the applicability of the present method. Then some new results of the electric potentials and displacements are provided. Numerical results show that the electrostatic voltage is approximately linear in the thickness direction, while parabolic in the plate in-plane directions, for both the deflection and the electric voltage. These results are very useful for distributed sensing and finite element verification.
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WenJie Yuan,Xinqing Zhao,Li-Jie Chen,Feng-Wu Bai 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
Ethanol production from Jerusalem artichoke tubers through a consolidated bioprocessing (CBP) strategy using the inulinase-producing yeast Kluyveromyces marxianus is an economical and competitive than that from a grainbased feedstock. However, poor inulinase production under ethanol fermentation conditions significantly prolongs the fermentation time and compromises ethanol productivity. Improvement of inulinase activity appears to be promising for increasing ethanol production from Jerusalem artichoke tubers by CBP. In the present study, expression of the inulinase gene INU with its own promoter in K. marxianus (K/INU2) was explored using the integrative cassette. Overexpression of INU was explored using chromosome integration via the HO locus of the yeast. Inulinase activity and ethanol were determined from inulin and Jerusalem artichoke tubers under fed-batch operation. Inulinase activity was 114.9 U/mL under aerobic conditions for K/INU2, compared with 52.3 U/mL produced by the wild type strain. Importantly, inulinase production was enhanced in K/INU2 under ethanol fermentation conditions. When using 230 g/L inulin and 220 g/L Jerusalem artichoke tubers as substrates, inulinase activities of 3.7 and 6.8 U/mL,respectively, were measured using K/INU2, comparing favorably with 2.4 and 3.1 U/mL, respectively, using the wide type strain. Ethanol concentration and productivity for inulin were improved by the recombinant yeast to 96.2 g/L and 1.34 g/L/h, respectively, vs 93.7 g/L and 1.12 g/L/h,respectively, by the wild type strain. Ethanol concentration and productivity improvements for Jerusalem artichoke tubers were 69 g/L and 1.44 g/L/h, respectively, from the recombinant strain vs 62 g/L and 1.29 g/L/h, respectively,from the wild type strain.
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The applicability study and validation of TULIP code for full energy range spectrum
Chen Wenjie,Du Xianan,Wang Rong,Zheng Youqi,Wang Yongping,Wu Hongchun 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.12
NECP-SARAX is a neutronics analysis code system for advanced reactor developed by Nuclear Engineering Computational Physics Laboratory of Xi’an Jiaotong University. In past few years, improvements have been implemented in TULIP code which is the cross-section generation module of NECP-SARAX, including the treatment of resonance interface, considering the self-shielding effect in non-resonance energy range, hyperfine group method and nuclear library with thermal scattering law. Previous studies show that NECP-SARAX has high performance in both fast and thermal spectrum system analysis. The accuracy of TULIP code in fast and thermal spectrum system analysis is demonstrated preliminarily. However, a systematic verification and validation is still necessary. In order to validate the applicability of TULIP code for full energy range, 147 fast spectrum critical experiment benchmarks and 170 thermal spectrum critical experiment benchmarks were selected from ICSBEP and used for analysis. The keff bias between TULIP code and reference value is less than 300 pcm for all fast spectrum benchmarks. And that bias keeps within 200 pcm for thermal spectrum benchmarks with neutronmoderating materials such as polyethylene, beryllium oxide, etc. The numerical results indicate that TULIP code has good performance for the analysis of fast and thermal spectrum system
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Lu Yi,Wu Jiachuan,Hu Minhui,Zhong Qinghua,Er Limian,Shi Huihui,Cheng Weihui,Chen Ke,Liu Yuan,Qiu Bingfeng,Xu Qiancheng,Lai Guangshun,Wang Yufeng,Luo Yuxuan,Mu Jinbao,Zhang Wenjie,Zhi Min,Sun Jiachen 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.6
Background/Aims: The accuracy of endosonographers in diagnosing gastric subepithelial lesions (SELs) using endoscopic ultrasonography (EUS) is influenced by experience and subjectivity. Artificial intelligence (AI) has achieved remarkable development in this field. This study aimed to develop an AI-based EUS diagnostic model for the diagnosis of SELs, and evaluated its efficacy with external validation. Methods: We developed the EUS-AI model with ResNeSt50 using EUS images from two hospitals to predict the histopathology of the gastric SELs originating from muscularis propria. The diagnostic performance of the model was also validated using EUS images obtained from four other hospitals. Results: A total of 2,057 images from 367 patients (375 SELs) were chosen to build the models, and 914 images from 106 patients (108 SELs) were chosen for external validation. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the model for differentiating gastrointestinal stromal tumors (GISTs) and non-GISTs in the external validation sets by images were 82.01%, 68.22%, 86.77%, 59.86%, and 78.12%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy in the external validation set by tumors were 83.75%, 71.43%, 89.33%, 60.61%, and 80.56%, respectively. The EUS-AI model showed better performance (especially specificity) than some endosonographers. The model helped improve the sensitivity, specificity, and accuracy of certain endosonographers. Conclusions: We developed an EUS-AI model to classify gastric SELs originating from muscularis propria into GISTs and non-GISTs with good accuracy. The model may help improve the diagnostic performance of endosonographers. Further work is required to develop a multi-modal EUS-AI system.
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Linxiang Tan,Wenjie Wu,Qiaoqiao Yin,Xiaoxia Ke,Ru Qiao,Guoxiu Tong,Lanlan Zhu 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.52 No.-
The dimer-type heterostructured Ag/Zn0.995Ni0.005O microspheres were prepared via two-stepmicrowave-assisted process, which exhibited stronger visible-light-activated antibacterial performancethan visible-light only, ZnO/Vis, Zn0.995Ni0.005O/Vis, or AgNPs/Vis. It is attributed to not only theinteraction of released metal ions with functional groups of vital enzymes and proteins, but also thecollapsing force of reactive oxygen species to the cells. Photoelectrochemical measurements show thatthe photogenerated charges of Ag/Zn0.995Ni0.005O possess longer lifetime and higher separation resultingfrom the synergistic effect between Ni and Ag, which leads to its superior visible-light-drivenantibacterial performance. Gram-negative bacteria are more effectively restrained by Ag/Zn0.995Ni0.005Othan Gram-positive bacteria because of structural difference between their cell walls.
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