Supragingival Plaque Microbial Community Analysis of Children with Halitosis^s

( Wen Ren ),( Qun Zhang ),( Xuenan Liu ),( Shuguo Zheng ),( Lili Ma ),( Feng Chen ),( Tao Xu ),( Baohua Xu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

As one of the most complex human-associated microbial habitats, the oral cavity harbors hundreds of bacteria. Halitosis is a prevalent oral condition that is typically caused by bacteria. The aim of this study was to analyze the microbial communities and predict functional profiles in supragingival plaque from healthy individuals and those with halitosis. Ten preschool children were enrolled in this study; five with halitosis and five without. Supragingival plaque was isolated from each participant and 16S rRNA gene pyrosequencing was used to identify the microbes present. Samples were primarily composed of Actinobacteria, Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, and Candidate phylum TM7. The α and β diversity indices did not differ between healthy and halitosis subjects. Fifteen operational taxonomic units (OTUs) were identified with significantly different relative abundances between healthy and halitosis plaques, and included the phylotypes of Prevotella sp., Leptotrichia sp., Actinomyces sp., Porphyromonas sp., Selenomonas sp., Selenomonas noxia, and Capnocytophaga ochracea. We suggest that these OTUs are candidate halitosis-associated pathogens. Functional profiles were predicted using PICRUSt, and nine level-3 KEGG Orthology groups were significantly different. Hub modules of co-occurrence networks implied that microbes in halitosis dental plaque were more highly conserved than microbes of healthy individuals` plaque. Collectively, our data provide a background for the oral microbiota associated with halitosis from supragingival plaque, and help explain the etiology of halitosis.

Exploring the oral microflora of preschool children

Wen Ren,Qun Zhang,Xuenan Liu,Shuguo Zheng,Lili Ma,Feng Chen,Tao Xu,Baohua Xu 한국미생물학회 2017 The journal of microbiology Vol.55 No.7

The oral cavity is one of the most important and complicated habitats in our body and supports diverse microbial communities. In this study, we aimed to determine the bacterial diversity and composition of various oral micro-niches. Samples were collected from supragingival plaque, saliva, and tongue coating from 10 preschool children (30 samples total). 16S rRNA gene pyrosequencing dataset generated 314,639 clean reads with an average of 10,488 ± 2,787 reads per sample. The phyla Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria were predominant, accounting for more than 90% of the total sequences. We found the highest α diversity, microbial richness, and evenness in plaque, compared with saliva and tongue coating. Plaque was also distinguished from saliva and tongue coating by phylogenetic distances (weighted UniFrac). Taxa with different relative abundances were further identified, confirming the existence of microbial differences across the three niches. Core microbiomes were defined of each niche; however, only a small proportion of operational taxonomic units (8.07%) were shared by the three niches. Coaggregation between Actinomyces spp. and Streptococcus spp. and other correlations among periodontal pathogens, such as Prevotella, Fusobacteria, Capnocytophaga, and Tannerella, were shown by a co-occurrence network. In summary, our study provides a framework of oral microbial communities in the population of preschool children as a baseline for further studies of oral diseases related to microbes.

Lycorine: A Potential Broad-Spectrum Agent Against Crop Pathogenic Fungi

( Jin Wen Shen ),( Yuan Ruan ),( Wei Ren ),( Bing Ji Ma ),( Xiao Long Wang ),( Chun Feng Zheng ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

A screening test showed that lycorine exhibited significant antifungal activity against 24 pathogenic crop fungi at concentrations of 500μg/ml and 100μg/ml, respectively. Fusarium graminearum was selected for antifungal mechanism studies by observing its mycelial morphology and investigating the variations in its conductivity. In addition, the substance absorption and metabolism of F. graminearum were explored. The mechanism was revealed as being one by which lycorine destroyed the cellular membrane and further influenced substance absorption and cell metabolism.

Prediction of exploration targets based on integrated analyses of source rock and simulated hydrocarbon migration direction: a case study from the gentle slope of Shulu Sag, Bohai Bay Basin, northern China

Changqing Ren,Fugui He,Xianzhi Gao,Dongsheng Wu,Wenli Yao,Jianzhang Tian,Huiping Guo,Yuanxin Huang,Li Wang,Han Feng,Junwei Li 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6

The Shulu Sag which is a rifted sag with NNE trend is located in the south of Jizhong Depression, Bohai Bay Basin, northern China. The gentle slope and three troughs are situated in the west and east of the sag, respectively. Both of the lower part of Shasan Member (Es3x) and the lower part of Shayi Member (Es1x) act as source rocks in this sag. Researches on the type, quantity, quality and thermal maturity of the respective organic matter have been conducted using Rock-Eval pyrolysis data. Type II is the dominant kerogen in Es1x of all troughs. However, Type II1 and III is the dominant kerogen in Es3x of Middle-Southern and Northern trough, respectively. TOC (total organic carbon) and pyrolysis S2 (hydrocarbon) values suggest that the Es1x source rocks in Middle-Southern and Northern trough are fair to good and poor to fair generative potential of hydrocarbon, separately. The Es3x source rocks in Middle-Southern and Northern trough possess fair to excellent and poor to fair generative potential of hydrocarbon, individually. Tmax (pyrolysis temperature at maximum S2) values indicate that most of Es3x samples are thermally mature, but all Es1x samples are thermally immature. Under large scale condition, the hydrocarbon secondary migration in the upper part of Shasan Member (Es3s), Shaer Member (Es2) and the upper part of Shayi Member (Es1s) have been simulated using fluid potential model with Arcgis 9.3 software. The simulation results reveal the direction of hydrocarbon secondary migration and the distribution of hydrocarbon migration-accumulation units (HMAUS), and also suggest that the hydrocarbon migration direction is obviously controlled by nose-like structure belts where most of hydrocarbons accumulate. That shows high reliability because they are consistent with the hydrocarbon exploration result in this area. On the basis of integrated analyses of source rocks and hydrocarbon migration direction, the following five areas in the gentle slope are identified to be the preferred hydrocarbon accumulation area: Taijiazhuang area, northern and southern Xicaogu area, as well as northern and southern Leijiazhuang area. It is considerably helpful to reduce the risk in hydrocarbon exploration of Shulu Sag.

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Research on a Multi-Objective Control Strategy for Current-source PWM Rectifiers under Unbalanced and Harmonic Grid Voltage Conditions

Yi-Wen Geng,Hai-Wei Liu,Ren-Xiong Deng,Fang-Fang Tian,Hao-Feng Bai,Kai Wang 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

Unbalanced and distorted grid voltages cause the grid side current of a current source PWM rectifier to be heavily distorted. They can also cause the DC-link current to fluctuate with a huge amplitude. In order to enhance the performance of a current-source PWM rectifier under unbalanced and harmonic grid voltage conditions, a mathematical model of a current-source PWM rectifier is established and a flexible multi-objective control strategy is proposed to control the DC-link current and grid-current. The fundamental positive/negative sequence, 5th and 7th order harmonic components of the grid voltage are first separated with the proposed control strategy. The grid current reference are optimized based on three objectives: 1) sinusoidal and symmetrical grid current, 2) sinusoidal grid current and elimination of the DC-current 2nd order fluctuations, and 3) elimination of the DC-current 2nd and 6th order fluctuations. To avoid separation of the grid current components, a multi-frequency proportional-resonant controller is applied to control the fundamental positive/negative sequence, 5th and 7th order harmonic current. Finally, experimental results verify the effectiveness of proposed control strategy.

A pellet-type optical nanomaterial of silica-based naphthalimide–DPA–Cu(<small>II</small>) complexes: recyclable fluorescence detection of pyrophosphate

Zhang, Jun Feng,Park, Minsung,Ren, Wen Xiu,Kim, Youngmee,Kim, Sung Jin,Jung, Jong Hwa,Kim, Jong Seung Royal Society of Chemistry 2011 Chemical communications Vol.47 No.12

<P>A pellet-type nanomaterial prepared from a mesoporous silica-immobilized naphthalimide–DPA–Cu(<SMALL>II</SMALL>) complex (MSIND–Cu) is investigated as a selective fluorescent chemosensor for PPi detection with a large “off–on” fluorescence change, low detection limit, short response time and a broad pH test range.</P> <P>Graphic Abstract</P><P>A pellet-type nanomaterial prepared from a mesoporous silica-immobilized naphthalimide–DPA–Cu(<SMALL>II</SMALL>) complex is investigated as a selective fluorescent chemosensor for PPi detection. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc00021g'> </P>

CK2 phosphorylates AP-2α and increases its transcriptional activity

( Kai Qun Ren ),( Shuang Lin Xiang ),( Fang Li He ),( Wen Feng Zhang ),( Xiao Feng Ding ),( Yan Yang Wu ),( Li Ping Yang ),( Jian Lin Zhou ),( Xiang Gao ),( Jian Zhang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.7

Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment, we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner. Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.

Research on a Multi-Objective Control Strategy for Current-source PWM Rectifiers under Unbalanced and Harmonic Grid Voltage Conditions

Geng, Yi-Wen,Liu, Hai-Wei,Deng, Ren-Xiong,Tian, Fang-Fang,Bai, Hao-Feng,Wang, Kai The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.1

Unbalanced and distorted grid voltages cause the grid side current of a current source PWM rectifier to be heavily distorted. They can also cause the DC-link current to fluctuate with a huge amplitude. In order to enhance the performance of a current-source PWM rectifier under unbalanced and harmonic grid voltage conditions, a mathematical model of a current-source PWM rectifier is established and a flexible multi-objective control strategy is proposed to control the DC-link current and grid-current. The fundamental positive/negative sequence, $5^{th}$ and $7^{th}$ order harmonic components of the grid voltage are first separated with the proposed control strategy. The grid current reference are optimized based on three objectives: 1) sinusoidal and symmetrical grid current, 2) sinusoidal grid current and elimination of the DC-current $2^{nd}$ order fluctuations, and 3) elimination of the DC-current $2^{nd}$ and $6^{th}$ order fluctuations. To avoid separation of the grid current components, a multi-frequency proportional-resonant controller is applied to control the fundamental positive/negative sequence, $5^{th}$ and $7^{th}$ order harmonic current. Finally, experimental results verify the effectiveness of proposed control strategy.

Fabrication of polyacrylic acid-based composite binders with strong binding forces on copper foils for silicon anodes in lithium-ion batteries

Ting-Ting Su,Wen-Feng Ren,Jiang-Meng Yuan,Ke Wang,Bing-Yu Chi,Run-Cang Sun 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

The commercial application of silicon (Si) anodes with high theoretical capacity is hampered by the poorcyclic stability because the huge volume change of Si during discharge/charge processes results in thepulverization of electrode materials and the electric contact loss of electrode materials with copper(Cu) foils. Binders play an important role for adhering active materials and conductive additives togetheronto Cu foils. Herein, we design and develop a three-dimensional networked composite binder (PAA-co-SN) via an amidation reaction between polyacrylic acid (PAA) and thiourea (SN). The granular anchors(CuxS) are constructed at the interface between PAA-co-SN binders and Cu foils, resulting from the reactionof S and Cu during the drying processes of Si electrodes. When used as binders for Si nanoparticles,they exhibit stable cyclic performance (1580 mAh g1 after 500 cycles). The anchoring mechanismbetween composite binders and Cu foils provides a strategy for the improvement of electrochemicalproperties of Si-based anodes for lithium-ion batteries.