Cilostazol ameliorates diabetic nephropathy by inhibiting highglucose-induced apoptosis

Chien-Wen Chian,Yung-Shu Lee,Yi-Ju Lee,Ya-Hui Chen,Chi-Ping Wang,Wen-Chin Lee,Huei-Jane Lee 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.5

Diabetic nephropathy (DN) is a hyperglycemia-induced progressive development of renal insufficiency. Excessive glucose can increase mitochondrial reactive oxygen species (ROS) and induce cell damage, causing mitochondrial dysfunction. Our previous study indicated that cilostazol (CTZ) can reduce ROS levels and decelerate DN progression in streptozotocin (STZ)-induced type 1 diabetes. This study investigated the potential mechanisms of CTZ in rats with DN and in high glucose-treated mesangial cells. Male Sprague-Dawley rats were fed 5 mg/kg/day of CTZ after developing STZ-induced diabetes mellitus. Electron microscopy revealed that CTZ reduced the thickness of the glomerular basement membrane and improved mitochondrial morphology in mesangial cells of diabetic kidney. CTZ treatment reduced excessive kidney mitochondrial DNA copy numbers induced by hyperglycemia and interacted with the intrinsic pathway for regulating cell apoptosis as an antiapoptotic mechanism. In high-glucose-treated mesangial cells, CTZ reduced ROS production, altered the apoptotic status, and down-regulated transforming growth factor beta (TGF-β) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB). Base on the results of our previous and current studies, CTZ deceleration of hyperglycemia-induced DN is attributable to ROS reduction and thereby maintenance of the mitochondrial function and reduction in TGF-β and NF-κB levels.

Immobilization of Proteins on Magnetic Nanoparticles

Wen-Chien Lee,Tzu-Hsien Wang 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4

Magnetic nanoparticles prepared from an alkaline solution of divalent and trivalent iron ions could covalently bind protein via the activation of N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC). Trypsin and avidin were taken as the model proteins for the formation of protein-nanoparticle conjugates. The immobilized yield of protein increased with molar ratio of EDC/nanoparticle. Higher concentrations of added protein could yield higher immobilized protein densities on the particles. In contrast to EDC, the yields of protein immobilization via the activation of cyanamide were relatively lower. Nanoparticles bound with avidin could attach a single-stranded DNA through the avidin-biotin interaction and hybridize with a DNA probe. The DNA hybridization was confirmed by fluorescence microscopy observations. Immobilized DNA on nanoparticles by this technique may have widespread applicability to the detection of specific nucleic acid sequence and targeting of DNA to particular cells.

Impact of Interleukin-10 Gene Polymorphisms on Survival in Patients with Colorectal Cancer

Wen-Chien Ting,Lu-Min Chen,Li-Chia Huang,Mann-Jen Hour,Yu-Hsuan Lan,Hong-Zin Lee,Bang-Jau You,Ta-Yuan Chang,Bo-Ying Bao 대한의학회 2013 Journal of Korean medical science Vol.28 No.9

Chronic inflammation is thought to be the leading cause of colorectal cancer, and interleukin-10 (IL10) has been identified as a potent immunomodulatory cytokine that regulates inflammatory responses in the gastrointestinal tract. Although several single nucleotide polymorphisms (SNPs) in IL10 have been associated with the risk of colorectal cancer, their prognostic significance has not been determined. Two hundred and eightytwo colorectal cancer patients were genotyped for two candidate cancer-associated SNPs in IL10. The associations of these SNPs with distant metastasis-free survival and overall survival were evaluated by Kaplan-Meier analysis and Cox regression model. The minor homozygote GG genotype of IL10 rs3021094 was significantly associated with a 3.30-fold higher risk of death compared with the TT+TG genotypes (P = 0.011). The patients with IL10rs3021094 GG genotype also had a poorer overall survival in Kaplan-Meier analysis (log-rank P = 0.007) and in multivariate Cox regression model (P = 0.044) adjusting for age, gender,carcinoembryonic antigen levels, tumor differentiation, stage, lymphovascular invasion,and perineural invasion. In conclusion, our results suggest that IL10 rs3021094 might be a valuable prognostic biomarker for colorectal cancer patients.

Effect of dopants on the structural, optical and electrical properties of solegel derived ZnO semiconductor thin films

Chien-Yie Tsay,Wen-Che Lee 한국물리학회 2013 Current Applied Physics Vol.13 No.1

Undoped, Ga-, In-, Zr-, and Sn-doped ZnO transparent semiconductor thin films were deposited on alkalifree glasses by solegel method. 2-methoxyethanol (2-ME) and diethanolamine (DEA) were chosen as a solvent and a stabilizer, respectively. The doping concentrationwas maintained at 2 at.% in the impurity doping precursor solutions. The effects of different dopants on the structural, optical, and electrical properties of ZnO thin films were investigated. XRD results show that all annealed ZnO-based thin films had a hexagonal (wurtzite) structure. ZnO thin films doped with impurity elements obviously improved the surface flatness and enhanced the optical transmittance. All impurity doped ZnO thin films showed high transparency in the visible range (>91%). The Ga- and In- doped ZnO thin films exhibited higher Hall mobility and lower resistivity than did the undoped ZnO thin film.

Abstract : Arsenic sequestration in iron plaque and its effect on As uptake by rice plants grown in paddy soils with high contents of As, iron oxides, and organic matter

( Chien Hui Syu ),( Pei Yu Jiang ),( Hsuan Han Huang ),( Wen Ting Chen ),( Tzu Huei Lin ),( Dar Yuan Lee ) 한국환경농학회 2012 한국환경농학회 워크샵자료 Vol.2012 No.2

The iron plaque formed on rice root has been confirmed to be a barrier on the uptake of arsenic in many hydroponic experiments. However, few studies provide the information about the relationship between soil characteristics, iron plaque formation and As uptake by rice in Ascontaminated soils. Therefore, the aim of this study is to investigate the effect of the rice root`s iron plaques on the As uptake by rice plants grown in geologically As-contaminated soils with high contents of iron oxides and organic matter from the Guandu Plain of northern Taiwan. A soil flooding incubation study was performed and a pot experiment was conducted. The rice seedling was pre-cultivated in solution cultures and then transplanted in three level As-contaminated soils for growing 39 days. The amounts of iron plaque on rice root were determined by extraction using dithionite-citrate-bicarbonate (DCB). The As species on iron plaque and the concentrations of As and Fe in iron plaque, root, root base and shoot of rice plants were determined. The results of the soil incubation study showed that both As and Fe concentrations in the soil solutions increased with flooding time due to reductive dissolution of iron oxides induced by high contents of organic matter in soils. High amounts of iron plaque were deposited on rice roots and large amounts of As were sequestrated in these iron plaques. Results of the As K-edge X-ray absorption nearedge spectroscopy (XANES) indicated that arsenate was the main species of arsenic sorbed on iron plaque of rice roots. About 74.0 to 93.2 % of total As released from soils were distributed in the iron plaques and only small proportion was distributed in the rice plants. This study provides evidence that the iron plaques of the rice roots was the main controlling factor in limiting the uptake and accumulation of As into the rice plants grown in paddy soils with high contents of iron oxides and organic matter, meanwhile, it suggests that enhancement of iron plaque formation could be the approach used for reducing the uptake of As by paddy rice grown in As-contaminated soils.

Enhancing the Correct Rate of Nasopharyngeal Fiberscope Reprocessing

Wen-Hsuan Li,Cheng-Chien Chen,Chia-Ling Yeh,Chia-Lun Lee 한국간호과학회 2019 한국간호과학회 학술대회 Vol.2019 No.10

Comparison of Short- and Long-term Hearing Outcomes of Successful Inlay Cartilage Tympanoplasty Between Small and Large Eardrum Perforations

Pei-Wen Wu,Wen-Hung Wang,Chi-Che Huang,Ta-Jen Lee,Chien-Chia Huang 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.4

Objectives. To compare the short- and long-term hearing outcomes after successful inlay cartilage tympanoplasty between patients with small (≤25%) and large (≥50%) eardrums perforations. Methods. This is a retrospective case series study conducted in a tertiary referral center. Twenty-five patients who underwent 27 procedures were enrolled. Their mean age was 60.26 years (range, 42 to 76 years). The mean follow-up time was 18.86 months (range, 12.30 to 35.83 months). The preoperative, initial postoperative, and long-term hearing results in patients with total repair of the eardrum were analyzed. Results. In the small size group, the average (±standard deviation) air-bone gap (ABG) closure was 1.08±7.53 dB in the short-term and 2.33±11.56 dB in the long-term hearing examinations. There was no difference between short- and long-term ABG closure (P=0.689). In the large size group, the average ABG closure was 9.77±9.40 dB in the short-term and 16.25±6.01 dB in the long-term hearing examinations. There was a significant difference between short- and long-term ABG closure (P=0.029). Conclusion. Patients with large perforations have continuous hearing improvement and ABG closure for more than one year. In contrast, the short- and long-term postoperative ABGs are almost the same in patients with small perforations. More long-term postoperative follow-up of hearing results is necessary for large perforations.

Affinity Separations Using Microfabricated Microfluidic Devices: In Situ Photopolymerization and Use in Protein Separations

Kelvin H. Lee,Chen Li,Wen-Chien Lee 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4

The use of microfabricated microfluidic devices offers significant advantages over current technologies including fast analysis time and small reagent requirements. In the context of proteomic research, the possibility of using affinity-based separations for prefractionation of samples using microfluidic devices has significant potential. We demonstrate the use of microscale devices to achieve affinity separations of proteins using a device fabricated from borosilicate glass wafers. Photolithography and wet etching are used to pattern individual glass wafers and the wafers are fusion bonded at 650oC to obtain enclosed channels. A polymer has been successfully polymerized in situ and used either as a frit for packing beads or, when derivatized with Cibacron Blue 3GA, as a separation matrix. Both of these technologies are based on in situ UV photopolymerization of glycidyl methacrylate (GMA) and trimethylolpropane trimethacrylate (TRIM) in channels.

Poster Session : PS 0390 ; Infectious Disease ; Clinical Characteristics and Effect of Empirical Therapy in Cirrhotic Adults with Community-Onset Bacteremia Visiting the Emergency Department(ED)

( Ching Chi Lee ),( Ming Yuan Hong ),( Wen Chien Ko ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Bacteremia, a indicative of a severe bacterial infection, has been associated with varied mortality rate, up to 37% in cirrhotic patients. This study was to investigate the clinical characteristics of community-onset bacteremia in cirrhotic adults and the clinical impact of appropriate empirical antibiotics on their outcome. Methods: Cirrhotic adults with community-onset bacteremia visiting the ED from January 2005 to December 2009 were identifi ed retrospectively. Demographic characteristics, severity, pathogens, port of entry, antibiotic therapy and clinical outcome were collected form the chart record. The susceptibility were determined by the broth microdilution method. Results: Of a total 246 episodes in cirrhotic patients, the mean age was 62.7 years, and 167 patients (67.9%) were male. The major resource of bacteremia included intra-abdominal infections (112 patients, 45.5%), primary bacteremia (43, 17.5%), urinary tract infection (39, 15.9%) and soft-tissue infection (22, 8.9%).Of a total 258 bacteremia-causing microorganisms, Enterobactericaeae and Gram-negative isolates account for 72.1% (186 isolates) and 76% (196), respectively; Escherichia coli (82 isolate, 31.8%) and Klebsiella species (61, 23.6%) were the most frequently encountered microorganisms. The in vitro susceptibility rate of each drug for Gram-negative and total isolates were demonstrated in Table 1. In multivariate analyses, the following variables were associated independently with 28-day mortality: the length of delayed appropriateness >72 hours (odds ratio [OR], 4.29; P=0.003); high serum creatinine (>1.5 mg/dL; OR, 3.12 ; P=0.005) in the ED; severe sepsis (OR, 3.61; P=0.01); high Piitt score(= 4; OR, 2.66 ; P=0.04); pneumonia (OR, 5.44; P=0.02); and diabetes (OR, 3.54; P=0.004). Conclusions: For cirrhotic adults with community-onset bacteremia in the ED, the clinical characteristics of bacteremia was demonstrated. In practice, their outcomes were superior following treatment with appropriate antibiotics compared to treatment with inappropriate antibiotics empirical.

Immobilization of Proteins on Magnetic Nanoparticles

Wang, Tzu-Hsien,Lee, Wen-Chien The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4

Magnetic nanoparticles prepared from an alkaline solution of divalent and trivalent iron ions could covalently bind protein via the activation of Nethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC). Trypsin and avidin were taken as the model proteins for the formation of protein-nanoparticle conjugates. The immobilized yield of protein increased with molar ratio of EDC/nanoparticie. Higher concentrations of added protein could yield higher immobilized protein densities on the particles. In contrast to EDC, the yields of protein immobilization via the a ctivation of cyanamide were relatively lower. Nanoparticles bound with avidin could attach a single-stranded DNA through the avidin-biotin interaction and hybridize with a DNA probe. The DNA hybridization was confirmed by fluorescence microscopy observations. Immobilized DNA on nanoparticles by this technique may have widespread applicability to the detection of specific nucleic acid sequence and targeting of DNA to particular cells.