DETERMINATION OF AMINOGLYCOSIDE ANTIBIOTICS BY A COLORIMETRIC METHOD BASED ON THE AGGREGATION OF GOLD NANOPARTICLES

RUIYONG WANG,SHUMIN FAN,RUIQIANG WANG,RUI WANG,HUANJING DOU,LVJING WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.4

A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL-1, 13.33–66.67 ng mL-1 and 20–180 ng mL-1, respectively. The corresponding limit of detection (3σ) was 0.354 ng mL-1, 0.999 ng mL-1 and 0.579 ng mL-1, respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.

Raw Inonotus obliquus polysaccharide counteracts Alzheimer’s disease in a transgenic mouse model by activating the ubiquitin-proteosome system

Wang Shumin,Dong Kaiye,Zhang Ji,Chen Chaochao,Shuai Hongyan,Yu Xin 한국영양학회 2023 Nutrition Research and Practice Vol.17 No.6

BACKGROUND/OBJECTIVES: Inonotus obliquus has been used as antidiabetic herb around the world, especially in the Russian and Scandinavian countries. Diabetes is widely believed to be a key factor in Alzheimer’s disease (AD), which is widely considered to be type III diabetes. To investigate whether I. obliquus can also ameliorate AD, it would be interesting to identify new clues for AD treatment. We tested the anti-AD effects of raw Inonotus obliquus polysaccharide (IOP) in a mouse model of AD (3×Tg-AD transgenic mice). MATERIALS/METHODS: SPF-grade 3×Tg-AD mice were randomly divided into three groups (Control, Metformin, and raw IOP groups, n = 5 per group). β-Amyloid deposition in the brain was analyzed using immunohistochemistry for AD characterization. Gene and protein expression of pertinent factors of the ubiquitin-proteasome system (UPS) was determined using real-time quantitative polymerase chain reaction and Western blotting. RESULTS: Raw IOP significantly reduced the accumulation of amyloid aggregates and facilitated UPS activity, resulting in a significant reduction in AD-related symptoms in an AD mouse model. The presence of raw IOP significantly enhanced the expression of ubiquitin, E1, and Parkin (E3) at both the mRNA and protein levels in the mouse hippocampus. The mRNA level of ubiquitin carboxyl-terminal hydrolase isozyme L1, a key factor involved in UPS activation, also increased by approximately 50%. CONCLUSIONS: Raw IOP could contribute to AD amelioration via the UPS pathway, which could be considered as a new potential strategy for AD treatment, although we could not exclude other mechanisms involved in counteracting AD processing.

Exosomal CircPRRX1 Enhances Doxorubicin Resistance in Gastric Cancer by Regulating MiR-3064-5p/PTPN14 Signaling

Shumin Wang,Mei Ping,Bin Song,Yarong Guo,Yuanfei Li,Junmei Jia 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.9

Purpose: Gastric cancer (GC) is a malignant tumor with a high mortality rate. Drug resistance is a major obstacle to GC therapy. This study aimed to investigate the role and mechanism of exosomal circPRRX1 in doxorubicin resistance in GC. Materials and Methods: HGC-27 and AGS cells were exposed to different doses of doxorubicin to construct doxorubicin-resistantcell lines. Levels of circPRRX1, miR-3064-5p, and nonreceptor tyrosine phosphatase 14 (PTPN14) were detected by quantitativereal-time PCR or Western blot assay. Then, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, transwell,and Western blot assays were used to explore the function of circPRRX1 in GC cells. Interactions among circPRRX1, miR-3064-5p,and PTPN14 were confirmed by dual-luciferase reporter assay. The in vivo function of circPRRX1 was analyzed in a xenograft tumormodel. Results: CircPRRX1 was highly expressed in doxorubicin-resistant GC cell lines. Knockdown of circPRRX1 reversed doxorubicinresistance in doxorubicin-resistant GC cells. Additionally, extracellular circPRRX1 was carried by exosomes to spread doxorubicinresistance. CircPRRX1 silencing reduced doxorubicin resistance by targeting miR-3064-5p or regulating PTPN14. In GC patients,high levels of circPRRX1 in serum exosomes were associated with poor responses to doxorubicin treatment. Moreover, depletionof circPRRX1 reduced doxorubicin resistance in vivo. Conclusion: CircPRRX1 strengthened doxorubicin resistance by modulating miR-3064-5p/PTPN14 signaling and might be atherapeutic target for GC patients.

The Analysis and Application of Competition and Cooperation between the Bus Lines

Shumin Feng,Xianglong Sun,Dixin Wang 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4

It is very valuable for planning bus routes and improving transport efficiency to quantify the cooperation and competition between lines. Cooperation modes and competition modes between two lines are defined respectively based on the location of bus stops. Cooperative coefficient and competitive coefficient are proposed to calculate different modes quantitatively in term of the number of overlapped stops and overlapped service area. These coefficients can be used to convert multi-relation transit network into weighted single network to analyze the close degree between lines. Taking Qiqihar City transit network as an example, cooperative coefficient and competitive coefficient between two lines are calculated. The bus network is converted into weighted single network, and the single network is then clustered with social network analysis method. The cluster result indicates that the network can be divided into three groups whose bus lines have different cooperation and competition strength, so distinguish management modes for different group are quite necessary.

Antibacterial and Hydrophilic Modification of PET Fabrics by Electron Beam Irradiation Process

Shumin Zhang,Fang Ding,Yingfeng Wang,Xuehong Ren,Tung-Shi Huang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.5

Electron beam (EB) irradiation has been utilized to modify materials for various applications due to its remarkableadvantages. As an efficient and environmental-friendly way for antibacterial and hydrophilic purposes, EB irradiation wasapplied to modify polyethylene terephthalate (PET) fabrics by grafting with a N-halamine precursor monomer 3-allyl-5,5-dimethylhydantoin (ADMH) and acrylic acid (AA) in this study. The grafted PET fabrics were loaded with silver ions tofurther enhance the antimicrobial efficacy. The hydrophilicity of the modified PET fabrics was evaluated by testing the watercontact angles with different contact times. The breaking strength and thermal stability of the modified swatches werestudied. The UVA light stability results showed the chlorine loading of the modified PET fabrics decreased with the extensionof UVA exposure time, and most chlorine loading could be recovered by re-chlorination. The antibacterial test showed thatthe modified PET swatches can inactivate all inoculated S. aureus and E. coli with short contact times.

Observer-based H∞ Control for Synchronization in Delayed Neural Networks Under Multiple Disturbances

Ting Wang,Jifeng Ge,Tao Li,Xin Chen,Shumin Fei 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.12

In this paper, the problem on master-slave synchronization is investigated for a class of delayed neural networks (DNNs) subject to multiple variable disturbances, where the synchronization can be widely utilized in many engineering fields such as teleoperation control, secure communication, and so on. Initially, the unknown disturbances are assumed to be generated by two external systems, in which the additional ones are required to belong to the space of L2[0,+∞). Then based on disturbance-observer-based-control method, H∞ control technique, and composite controller, a sufficient condition on designing the observer gains and controller ones are established to ensure that the overall closed-loop system is asymptotically stable with H∞ control performance. Furthermore, by using matrix transformation technique, the derived condition is converted into the linear matrix inequality (LMI)forms, which can be easily checked and present much less conservatism. Finally, some simulations and comparisons in an example are given to illustrate the effectiveness of our control methods.

Isolation of formaldehyde-degrading bacteria and the evaluation of the degradation characteristics

Menglei Wang,Shumin Xu,Shunyi Li,Rencheng Zhu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.75 No.-

A formaldehyde-degrading bacteria strain, B1, was isolated. Strain B1 was characterized morphologically,physiologically and biochemically, and it was identified as Pseudomonas putida. Then, the formaldehydebiodegradation characteristics were evaluated in a biofilter. A formaldehyde removal rate above 90% wasmaintained when inlet loading was below 38.9 mg L 1 h 1. In addition, strain B1 quickly becamedominant bacteria when the reactor was restarted following a 30-days’ disruption, illustrating that thestrain is both adaptable and resilient. The numerical simulation results demonstrate that the maximumdegradation rates in the biofilter with strain B1 are betterfitted with the Haldane model.

LncRNA MALAT1 protects human umbilical vein endothelial cells against ox-LDL triggered cell death through regulation of MGP

Xia Wang,Hongqin Zhao,Shaonan Yang,Xiaojun Shao,Shumin Nie,Xudong Pan 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.3

Backgrounds: Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is an lncRNA that has been suggested as a key regulator in the onset of atherosclerosis (AS). This study described the role of MALAT1 in oxidized low density lipoprotein (ox-LDL)-induced endothelial cells death. Methods: Human umbilical vein endothelial cells (HUVECs) were subjected to ox-LDL, before which the expression of MALAT1 was overexpressed by transfection. CCK-8 assay, flow cytometer detection, and western blot were carried out to evaluate cell viability, apoptosis and autophagy. qRT-PCR and western blot analyses were performed to investigate the regulatory relationship between MALAT1, Matrix Gla protein (MGP) and mTOR signaling to decode the underlying mechanism. Results: Up-regulation of MALAT1 attenuated ox-LDLinduced HUVECs lose, as evidenced by the promoted cell viability, and the decreased apoptosis rate. This finding was coupled with the down-regulated p53, Bax, active-caspase-3, Beclin-1 and LC3-II, as well as the up-regulated Bcl-2 and p62. Meanwhile, MALAT1 upregulation promoted the phosphorylation of p70S6K and mTOR, and the expression of MGP. MGP up-regulation exhibited MALAT1-like propoties in preventing ox-LDL-induced cell death and mTOR deactivation. Of contrast, MGP silence affected HUVECs survival and mTOR signaling resulted in contrary impacts. Conclusion: The present work described that MALAT1 up-regulation prevented ox-LDL-mediated apoptosis and autophagy in HUVECs. The protective effects of MALAT1 might be partially via up-regulating MGP, which led to the activation of mTOR signaling.

Finite-time Issue of Discrete-time Linear Switched Systems With Partial Finite-time Unstable Modes Based on an Inverse Weighted Switching Scheme

Yunpeng Zhan,Ruihua Wang,Shumin Fei 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

The work proposes a multiple convex Lyapunov function and an inverse weighted switching scheme to investigate the finite-time stability and finite-time boundedness for a class of discrete-time switched linear systems with partial finite-time unstable modes. A multiple convex Lyapunov function is put forth by constructing a convex combination of positive definite matrices, which can relax the restricted conditions of the Lyapunov function and make it carry more decision variables than traditional Lyapunov function methods. Besides, the inverse weighted switching scheme is devised by summing the reciprocal of each dwell time with weighting coefficients, by which tighter dwell time bounds are ensured. On the basis of the new Lyapunov function and switching scheme, the finitetime control for a class of switched linear systems with partial finite-time unstable modes is addressed. Different from other researches that require all subsystems to be controllable, we only require the existence of one controllable subsystem. In the end, two numerical examples and a tunnel diode circuit example are provided to verify the effectiveness of the developed results.

Exponential Synchronization for Arrays of Coupled Neural Networks with Time-delay Couplings

Tao Li,Ting Wang,Ai-guo Song,Shumin Fei 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.1

This paper deals with global exponential synchronization in arrays of coupled delayed neural networks with both delayed coupling and one single delayed one. Through employing Kronecker product and convex combination technique, two novel synchronization criteria are presented in terms of linear ma-trix inequalities (LMIs), and these conditions are dependent on the bounds of both time-delay and its derivative. Through employing Matlab LMI Toolbox and adjusting some matrix parameters in the derived results, we can realize the design and applications of the addressed systems, which shows that our methods improve and extend those reported methods. The efficiency and applicability of the proposed results can be demonstrated by three numerical examples with simulations.