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Poster Session : Characterization of DJ-1 Like gene in arabidopsis
( Wahyu Indra Duwi Fanata ),( Min Hee Jung ),( Joon Yung Cha ),( Netty Ermawati ),( Ying Shi Liang ),( Mukhamad Su`udi ),( Sun Ping ),( Sun Shin Cha ),( Kon Ho Lee ),( Dae Young Son ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-
RNA-dependent RNA polymerase 6 is required for efficient hpRNA-induced gene silencing in plants
Harmoko, Rikno,Fanata, Wahyu Indra Duwi,Yoo, Jae Yong,Ko, Ki Seong,Rim, Yeong Gil,Uddin, Mohammad Nazim,Siswoyo, Tri Agus,Lee, Seung Sik,Kim, Dool Yi,Lee, Sang Yeol,Lee, Kyun Oh Springer-Verlag 2013 Molecules and cells Vol.35 No.3
Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar
Anisa Maharani,Wahyu Indra Duwi Fanata,Faida Nur Laeli,김경민,Tri Handoyo 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.1
The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic properties and phenotypes. The anther culture techniques on indica black rice cultivar have a high difficulty factor to get plants, because of the low regeneration ability at the plant formation phase from the anther callus. This study aimed to investigate the influence of the cold-pretreatment time on anther, the combination of plant growth regulators (PGR’s) concentrations, and putrescine concentrations in media for the increase callus induction and plant regeneration of indica black rice. The optimization of the cold pre-treatment time was important to obtain the high-frequency callus induction, which showed that anther at the 4°C for 8 days formed the high callus induction (20%). To accelerate the callus induction, the application of 20 µM putrescine in the MS medium could produce more friable embryogenic callus for 24 days with 27% of callus formation. Generally, the optimal medium for the high frequency of callus induction contained 2 mgL-1 NAA+0.5 mgL-1 Kinetin+20 µM putrescine. Especially indica black rice cultivars, the best media to get a high plant regeneration frequency were N6 media containing the combination of 2 mgL-1 IAA and 2,5 mgL-1 Kinetin. The total callus regenerated to plantlet about 12.5%. The study of the callus induction and in-vitro plant regeneration medium for indica black rice were still important to develop to get the best result for other cultivars.
RNA-Dependent RNA Polymerase 6 Is Required for Efficient hpRNA-Induced Gene Silencing in Plants
RIKNOHARMOKO,이균오,Wahyu Indra Duwi Fanata,유재용,고기성,임영길,엠다나짐우단,Tri Agus Siswoyo,이승식,김둘이,이상열 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.3
In plants, transgenes with inverted repeats are used to in-duce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which con-verts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self-complementary hairpin RNA (hpRNA) does not require RDR6, because the hpRNA can directly fold back on itself to form dsRNA. However, it is unclear whether RDR6 plays a role in hpRNA-induced RNA silencing by amplifying dsRNA to spread RNA silencing within the plant. To address the efficiency of hpRNA-induced RNA silencing in the presence or absence of RDR6, Wild type (WT, Col-0) and rdr6-11 Arabidopsis thaliana lines expressing green fluorescent protein (GFP) were generated and transformed with a GFP-RNA interference (RNAi) construct. Whereas most GFP-RNAi-transformed WT lines exhibited almost complete silencing of GFP expression in the T1 generation, various levels of GFP expression remained among the GFP-RNAi-transformed rdr6-11 lines. Homozygous expression of GFP-RNAi in the T3 generation was not sufficient to induce complete GFP silencing in several rdr6-11 lines. Our results indicate that RDR6 is required for efficient hpRNA-induced RNA silencing in plants.
Dewi Puspa Arisandi,Fragaria Vesca Paradisa,Bambang Sugiharto,Sholeh Avivi,Wahyu Indra Duwi Fanata 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.4
Breeding using the double haploid approach has been used in accelerating the rice breeding process. However, the main obstacle in the application of this technique in rice is the low level of callus formation and their regeneration into haploid plants. Based on these issues, this research is aimed at developing the best method for obtaining double haploid plants through anther culture using three kinds of aromatic rice of Indonesian local varieties. Anthers of Pendok, Genjah Arum, and Merah Wangi varieties were cultured in callus induction media which were either supplemented with different types of carbon sources, or auxins and ethylene inhibitors. However, the application of different sugar types did not significantly affect callus formation of Pendok and Genjah Arum. However, auxin treatment showed NAA to be more efficient for the callus induction of Pendok, while 2,4-D had no eff ect on callus induction in all tested varieties. The addition of ethylene inhibitor successfully induced callus formation of Merah Wangi varieties, while the rates of Pendok and Genjah Arum were not signifi cantly aff ected. Callus of three plant lines were then regenerated into plantlet in regeneration media. Of the three callus varieties used, only Pendok could regenerate into plantlets and grow into adult haploid plants.
Yoo, Jae Yong,Ko, Ki Seong,Seo, Hyun-Kyeong,Park, Seongha,Fanata, Wahyu Indra Duwi,Harmoko, Rikno,Ramasamy, Nirmal Kumar,Thulasinathan, Thiyagarajan,Mengiste, Tesfaye,Lim, Jae-Min,Lee, Sang Yeol,Lee, American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.27
<P>The most abundant <I>N</I>-glycan in plants is the paucimannosidic <I>N</I>-glycan with core β1,2-xylose and α1,3-fucose residues (Man<SUB>3</SUB>XylFuc(GlcNAc)<SUB>2</SUB>). Here, we report a mechanism in <I>Arabidopsis thaliana</I> that efficiently produces the largest <I>N</I>-glycan in plants. Genetic and biochemical evidence indicates that the addition of the 6-arm β1,2-GlcNAc residue by <I>N</I>-acetylglucosaminyltransferase II (GnTII) is less effective than additions of the core β1,2-xylose and α1,3-fucose residues by XylT, FucTA, and FucTB in <I>Arabidopsis</I>. Furthermore, analysis of <I>gnt2</I> mutant and 35S:GnTII transgenic plants shows that the addition of the 6-arm non-reducing GlcNAc residue to the common <I>N</I>-glycan acceptor GlcNAcMan<SUB>3</SUB>(GlcNAc)<SUB>2</SUB> inhibits additions of the core β1,2-xylose and α1,3-fucose residues. Our findings indicate that plants limit the rate of the addition of the 6-arm GlcNAc residue to the common <I>N</I>-glycan acceptor as a mechanism to facilitate formation of the prevalent <I>N</I>-glycans with Man<SUB>3</SUB>XylFuc(GlcNAc)<SUB>2</SUB> and (GlcNAc)<SUB>2</SUB>Man<SUB>3</SUB>XylFuc(GlcNAc)<SUB>2</SUB> structures.</P>