Protective effect of polysaccharides from Celluclast-assisted extract of <i>Hizikia fusiforme</i> against hydrogen peroxide-induced oxidative stress <i>in vitro</i> in Vero cells and <i>in vivo</i> in zebrafish

Wang, Lei,Oh, Jae Young,Kim, Hyun Soo,Lee, WonWoo,Cui, Yongri,Lee, Hyo Geun,Kim, Yong-Tae,Ko, Jae Young,Jeon, You-Jin Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.112 No.-

<P><B>Abstract</B></P> <P>Polysaccharides were extracted by Celluclast-assisted hydrolysis and ethanol precipitation from <I>Hizikia fusiforme</I> (HFPS), and their <I>in vitro</I> antioxidant effects were evaluated in Vero cells. HFPS contains 63.56±0.32% sulfate polysaccharides, which comprise fucose (53.53%), galactose (23.15%), glucose (5.95%), and xylose (17.37%). HFPS scavenged DPPH, alkyl, and hydroxyl radicals at IC<SUB>50</SUB> values of 0.81±0.02, 0.25±0.02, and 0.21±0.03mg/mL, respectively. HFPS significantly reduced H<SUB>2</SUB>O<SUB>2</SUB>-induced cytotoxicity in Vero cells. Furthermore, HFPS reduced intracellular ROS levels, and H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis. HFPS has a strong protective effect against H<SUB>2</SUB>O<SUB>2</SUB>-stimulated oxidative stress <I>in vivo</I> in zebrafish, and showed improved survival rate, decreased heart rate, and reduced ROS generation and cell death. These results suggest that HFPS may be useful as an antioxidant in medical and cosmetic industries.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Polysaccharides of <I>Hizikia fusiforme</I> (HFPS) were obtained by Celluclast-assisted hydrolysis. </LI> <LI> HFPS contains 63.56±0.32% sulfate polysaccharide and constitutes by fucose (53.53%), galactose (23.15%), glucose (5.95%), and xylose (17.37%). </LI> <LI> HFPS possessed a protective effect against hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>)-induced oxidative stress <I>in vitro</I> Vero cells. </LI> <LI> HFPS inhibited H<SUB>2</SUB>O<SUB>2</SUB>-induced reactive oxygen species (ROS) generation, and cell death <I>in vivo</I> zebrafish. </LI> </UL> </P>

Active damage localization technique based on energy propagation of Lamb waves

Wang, Lei,Yuan, F.G. Techno-Press 2007 Smart Structures and Systems, An International Jou Vol.3 No.2

An active damage detection technique is introduced to locate damage in an isotropic plate using Lamb waves. This technique uses a time-domain energy model of Lamb waves in plates that the wave amplitude inversely decays with the propagation distance along a ray direction. Accordingly the damage localization is formulated as a least-squares problem to minimize an error function between the model and the measured data. An active sensing system with integrated actuators/sensors is controlled to excite/receive $A_0$ mode of Lamb waves in the plate. Scattered wave signals from the damage can be obtained by subtracting the baseline signal of the undamaged plate from the recorded signal of the damaged plate. In the experimental study, after collecting the scattered wave signals, a discrete wavelet transform (DWT) is employed to extract the first scattered wave pack from the damage, then an iterative method is derived to solve the least-squares problem for locating the damage. Since this method does not rely on time-of-flight but wave energy measurement, it is more robust, reliable, and noise-tolerant. Both numerical and experimental examples are performed to verify the efficiency and accuracy of the method, and the results demonstrate that the estimated damage position stably converges to the targeted damage.

Room-temperature columnar liquid crystal based on tetrathiafulvalene

Wang, Lei,Jeong, Kwang-Un,Lee, Myong-Hoon Royal Society of Chemistry 2008 Journal of materials chemistry Vol.18 No.23

<P>A series of novel TTF derivatives has been synthesized. Polarized optical microscopy (POM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD) techniques reveal the first room temperature columnar liquid crystal based on TTF.</P> <P>Graphic Abstract</P><P>The first room temperature columnar liquid crystal based on tetrathiafulvalene was synthesized. The highly ordered structure incorporating the tetrathiafulvalene unit can be a promising candidate for a wide range of electronic applications. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b805023f'> </P>

Corporate venture capital: technological innovation or value creation? A comparative study of CVC- and IVC-invested Chinese listed companies

Wang Lei,Zhou Fangzhao,An Yunbi,Yang Jun 기술경영경제학회 2019 ASIAN JOURNAL OF TECHNOLOGY INNOVATION Vol.27 No.3

This paper analyzes the effects of corporate venture capital (CVC) and independent venture capital (IVC) backing on technological innovation and value creation in invested companies. Using data on Chinese listed companies, we show that CVC investment can induce higher firm value than can IVC investment. By considering different dimensions of complementary assets, technological fit, geographical proximity, and product market relations, we explore the mechanism through which CVC contributes to innovation and value creation in invested companies in China.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Story of the monkey: The modular narrative and its origin of 〈The Uproar in Heaven〉

Wang, Lei 한국만화애니메이션학회 2012 만화애니메이션연구 Vol.- No.29

The essay explores the narrative structure of the classical Chinese feature animation, <The Uproar in Heaven> (a.k.a. Da Nao Tian Gong, 1964). The film is presented with a modular structure which is quite unique compared with the storytelling in feature animated films from other cultures, but could be connected with the tradition narrative structure in Chinese Zhanghui style novels in Ming and Qing Dynasty. By relating the original text of the story, the 16th century novel Journey to the West (a.k.a. Xi You Ji), with the film <The Uproar in Heaven>, the essay addresses the question of how the narrative tradition in Chinese classical literature influenced the Uproar in Heave for its segment narrative structure, character driven storytelling strategy and mirrored repetitive 2 plot lines. The subject of this essay is even more significant after the restored 3D version of <The Uproar in Heaven> was re-released in the spring of 2012 and became one of the best-selling animated feature film in the history of the country.

Network Coding for Energy-Efficient Distributed Storage System in Wireless Sensor Networks

( Wang Lei ),( Yang Yuwang ),( Zhao Wei ),( Lu Wei ) 한국인터넷정보학회 2013 KSII Transactions on Internet and Information Syst Vol.7 No.9

Anetwork-coding-basedschemeis proposed to improvethe energy efficiency of distributedstorage systemsinWSNs (Wireless Sensor Networks).Wemainly focuson two problems: firstly, consideration is given to effective distributed storage technology; secondly, we address how to effectively repair the data in failed storage nodes. For the first problem, we propose a method to obtainasparse generator matrix to construct network codes, and thissparse generator matrix is proven to be the sparsest. Benefiting from thismatrix, the energy consumption required to implement distributed storage is reduced. For the second problem, we designed a network-coding-based iterativerepair method, which adequately utilizes the idea of re-encoding at intermediate nodes from network codingtheory. Benefiting from the re-encoding, the energy consumption requiredby data repair issignificantly reduced. Moreover, we providean explicitlower bound of field size required by this scheme, which implies that itcan work over a small fieldand therequiredcomputation overhead is very low. The simulation resultverifiesthat the proposedschemenot only reducesthe total energy consumptionrequired to implement distributed storage systeminWSNs, but also balancesenergyconsumption of the networks.

Bioactivities of the edible brown seaweed, <i>Undaria pinnatifida</i>: A review

Wang, Lei,Park, Young-Jin,Jeon, You-Jin,Ryu, BoMi Elsevier 2018 Aquaculture Vol.495 No.-

<P><B>Abstract</B></P> <P>Natural ingredients containing high nutrients, various natural compounds, with broad spectrum of bioactivities and wide range of natural distribution or production, have been attracting more and more attention in food and pharmaceutical industries. <I>Undaria pinnatifida</I>, is an edible brown seaweed that contains high carbohydrate, protein, microelement, and various types of secondary metabolites, which possess strong bioactivities. It has been one of the popular seaweeds used for food as well as medicine in most of Asian countries including China, Korea, and Japan for a long time. Recent studies suggest that <I>U. pinnatifida</I> is rich in natural compounds such as polyphenols, peptides, and polysaccharides, these compounds possess various bioactivities such as anticancer, antioxidant, anti-inflammatory, and anti-diabetes properties. In the present review, we have collected and summarized the scientific data published about the bioactivities of <I>U. pinnatifida</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The chemical components of the edible brown seaweed, <I>Undaria pinnatifida</I> had been summarized. </LI> <LI> The bioactivities of the edible brown seaweed, <I>Undaria pinnatifida</I> had been summarized. </LI> <LI> Recent bioactivities research progress of the edible brown seaweed, <I>Undaria pinnatifida</I> had been discussed. </LI> </UL> </P>

Antioxidant effects of citrus pomace extracts processed by super-heated steam

Wang, Lei,Jo, Min-Ji,Katagiri, Riho,Harata, Kaori,Ohta, Moemi,Ogawa, Ayane,Kamegai, Masayuki,Ishida, Yasuyuki,Tanoue, Shota,Kimura, Sojiro,Lee, Seung-Cheol,Jeon, You-Jin Elsevier 2018 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.90 No.-

<P>This study was performed to investigate the enhanced antioxidant effects of citrus pomace (CP) extracts processed by super-heated steam (SHS). CP extracts were obtained using a closed SHS system at three different temperatures (100, 200, and 300 degrees C) with an extraction time of 10 or 20 min. These extracts were evaluated for their antioxidant activities by measuring their DPPH, alkyl, and hydroxyl radical scavenging activity using Electron Spin Resonance (ESR) spectroscopy and for their protective effects against H2O2-induced oxidative stress in Vero cells. All extracts exhibited strong radical scavenging activities and protective effects against H2O2-induced Vero cell damage. Especially, the SHS-CP extracts obtained at 300 degrees C with extraction times of both 10 min and 20 min exhibited antioxidant activities that were significantly higher than those of ethanol extracts and SHS-CP extracts obtained at other temperatures. The total phenolic and flavonoid contents of SHS-CP extracts and their antioxidant activities were found to be increased with elevated processing temperatures. Moreover, gas chromatography/mass spectrometry (GC/MS) in the presence of a methylating reagent revealed that some characteristic polyphenols and polyhydroxycyclohexanes were present in the SHS-CP extracts. Thus, this study demonstrated that SHS extraction is an efficient and environmentally friendly method for extracting antioxidants from CP.</P>

Design, Synthesis and Catalytic Property of L-Proline Derivatives as Organocatalysts for Direct Aldol Reaction

Wang, Lei,Tang, Ruiren,Yang, Hua Korean Chemical Society 2013 대한화학회지 Vol.57 No.5

A series of chiral prolinamide compounds with pyridine-2, 6-dicarboxylic acid moieties derived from L-proline have been designed and synthesized, their catalytic properties for direct asymmetric aldol reactions were also studied in this article. These catalysts gave the aldol product in high yield (87%) and high enantioselectivity, up to 85%, of the anti-structure at room temperature but gave disappointing results at a lower temperature or when additive was added. Conditions, including solvents, temperature and additives were screened for the reactions. Moreover, the influence of presence of water on yield and stereoselectivity was also discussed.