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RISS 인기검색어
- 검색키워드
- 저자 : Vijai, D (검색결과 14 건)
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Flexural behaviour of fibre reinforced geopolymer concrete composite beams
Vijai K,Kumutha R,Vishnuram B.G. 사단법인 한국계산역학회 2015 Computers and Concrete, An International Journal Vol.15 No.3
An experimental investigation on the behaviour of geopolymer composite concrete beams reinforced with conventional steel bars and various types of fibres namely steel, polypropylene and glass in different volume fractions under flexural loading is presented in this paper. The cross sectional dimensions and the span of the beams were same for all the beams. The first crack load, ultimate load and the load-deflection response at various stages of loading were evaluated experimentally. The details of the finite element analysis using "ANSYS 10.0" program to predict the load-deflection behavior of geopolymer composite reinforced concrete beams on significant stages of loading are also presented. Nonlinear finite element analysis has been performed and a comparison between the results obtained from finite element analysis (FEA) and experiments were made. Analytical results obtained using ANSYS were also compared with the calculations based on theory and presented.
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Vijai Singh Karwasara,Vinod Kumar Dixit 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The effect of culture medium nutrients ongrowth and alkaloid production by plant cell cultures ofNothapodytes nimmoniana (J. Grah.) Mabberley (Icacinaceae)was studied with a view to increasing the productionof the alkaloid camptothecin, a key therapeutic drug usedfor its anticancer properties. Amongst the various sugarstested with Murashige and Skoog (MS) medium, such asglucose, fructose, maltose, and sucrose, maximum accumulationof camptothecin was observed with sucrose. Highnitrate in the media supports the biomass, while highammonium enhances the camptothecin content. Selectivefeeding of 60 mM total nitrogen with a NH4?/NO3- balanceof 5/1 on day 15 of the culture cycle results in a 2.4-fold enhancement in the camptothecin content over thecontrol culture (28.5 lg/g DW). Furthermore, the sucrosefeeding strategy greatly stimulated cell biomass and camptothecinproduction. A modified MS medium was developedin the present study, which contained 0.5 mMphosphate, a nitrogen source feeding ratio of 50/10 mMNH4?/NO3- and 3 % sucrose with additional 2 % sucrosefeeding (added on day 12 of the cell culture cycle) with10.74 lM naphthaleneacetic acid and 0.93 lM kinetin. Finally, the selective medium has 1.7- and 2.3-fold higherintracellular and extracellular camptothecin content overthe control culture (29.2 and 8.2 lg/g DW), respectively.
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Vijai Singh,Dharmendra Kumar Chaudhary,Indra Mani,Rohan Jain,B.N. Mishra 한국미생물학회 2013 The journal of microbiology Vol.51 No.3
Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of diseases in fish, amphibians, reptiles, and humans. In fish it causes several disease symptoms including tail and skin rot, and haemorrhagic septicemia; in human it causes soft-tissue wound infection and diarrhoea. The pathogenesis of A. hydrophila is multifactorial, but the mechanism is unknown so far. It is considered to be mediated by expression and secretion of extracellular proteins such as aerolysin, lipase, chitinase, amylase, gelatinase, hemolysins, and enterotoxins. A number of the putative virulence-protein-encoding genes that are present in the genome of A. hydrophila have been targeted by PCR for molecular diagnosis. These significant genes are also targeted for over-production of proteins by cloning and expression methods. In this review, we emphasize recent progress in the cloning, expression, and regulation of putative virulence-protein-encoding genes of A. hydrophila for a better understanding of the pathogenesis and also help to provide effective strategies for control of diseases.
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Karwasara, Vijai Singh,Dixit, Vinod Kumar 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
The effect of culture medium nutrients on growth and alkaloid production by plant cell cultures of Nothapodytes nimmoniana (J. Grah.) Mabberley (Icacinaceae) was studied with a view to increasing the production of the alkaloid camptothecin, a key therapeutic drug used for its anticancer properties. Amongst the various sugars tested with Murashige and Skoog (MS) medium, such as glucose, fructose, maltose, and sucrose, maximum accumulation of camptothecin was observed with sucrose. High nitrate in the media supports the biomass, while high ammonium enhances the camptothecin content. Selective feeding of 60 mM total nitrogen with a $NH_4{^+}/NO_3{^-}$ balance of 5/1 on day 15 of the culture cycle results in a 2.4-fold enhancement in the camptothecin content over the control culture ($28.5{\mu}g/g$ DW). Furthermore, the sucrose feeding strategy greatly stimulated cell biomass and camptothecin production. A modified MS medium was developed in the present study, which contained 0.5 mM phosphate, a nitrogen source feeding ratio of 50/10 mM $NH_4{^+}/NO_3{^-}$ and 3 % sucrose with additional 2 % sucrose feeding (added on day 12 of the cell culture cycle) with $10.74{\mu}M$ naphthaleneacetic acid and $0.93{\mu}M$ kinetin. Finally, the selective medium has 1.7- and 2.3-fold higher intracellular and extracellular camptothecin content over the control culture (29.2 and $8.2{\mu}g/g$ DW), respectively.
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A simple, rapid and sensitive spectrofluorimetric method for the determination of camptothecin
Karwasara, Vijai Singh,Nahata, Alok,Dixit, Vinod Kumar 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.2
Camptothecin (CPT) represents a clinically useful class of anticancer agent. Proper identification and quantitation of the CPT in the plant extracts and in-vitro cell culture extracts is fundamental to assess the CPT content and its biosynthetic potential in plants. A simple, sensitive and rapid, spectrofluorimetric method has been developed and validated for the quantitative estimation of camptothecin. The method was validated in terms of linearity (2-20 ng/ml), precision (intra-day variation below 0.15, interday variation below 1.2), and accuracy (98.0 to 100.2%). The limit of detection and limit of quantification for CPT were found to be 0.10 ng/ml and 0.36 ng/ml, respectively. The developed spectrofluorimetric method provides a rapid and cost effective method for the routine analysis of CPT in plant extracts and tissue culture samples. The developed method was successfully used for the estimation of CPT in natural plant extracts and cell culture extracts. The Nothapodytes nimmoniana callus cells having nearly 3-fold higher CPT content over the leaf (0.005%) explant of the plant. The highest CPT content was found in the stem part (0.092%) followed by the fruit (0.088%). The method is simple, sensitive and precise; it can be used for the routine quality control testing of formulations containing CPT.
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Evaluation of protective coatings for geopolymer mortar under aggressive environment
Rathinam, Kumutha,Kanagarajan, Vijai,Banu, Sara Techno-Press 2020 Advances in materials research Vol.9 No.3
The aim of this study is to investigate the durability of fly ash based geopolymer mortar with and without protective coatings in aggressive chemical environments. The source materials for geopolymer are Fly ash and Ground Granulated Blast furnace Slag (GGBS) and they are considered in the combination of 80% & 20% respectively. Two Molarities of NaOH solution were considered such as 8M and 10M. The ratio of binder to sand and Sodium silicate to Sodium hydroxide solution (Na<sub>2</sub>SiO<sub>3</sub>/NaOH) are taken as 1:2 and 2 respectively. The alkaline liquid to binder ratio is 0.4. Compressive strength tests were conducted at various ages of the mortar specimens. In order to evaluate the performance of coatings on geopolymer mortar under aggressive chemical environment, the mortar specimens were coated with two different types of coatings such as epoxy and Acrylic. They were then subjected to different chemical environments by immersing them in 10% standard solutions of each ammonium nitrate, sodium chloride and sulphuric acid. Drop in compressive strength as a result of chemical exposure was considered as a measure of chemical attack and the drop in compressive strength was measured after 30 and 60 days of chemical exposure. The compressive strength results following chemical exposure indicated that the specimens containing the acrylic coating proved to be more resistant to chemical attacks. The control specimen without coating showed a much greater degree of deterioration. Therefore, the application of acrylic coating was invariably much more effective in improving the compressive strength as well as the resistance of mortar against chemical attacks. The results also indicated that among all the aggressive attacks, the sulphate environment has the most adverse effect in terms of lowering the strength.
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Dharmendra Kumar Soni,Durg Vijai Singh,Suresh Kumar Dubey 한국미생물학회 2015 The journal of microbiology Vol.53 No.9
cccListeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. The present study aimed at characterizing L. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and ERIC- and REP-PCR fingerprint analyses. The results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from vaginal swab (2200)] were positive for L. monocytogenes. All the isolates belonged to serogroup 4b, and were + ve for virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA, hlyA, and iap. Based on the mice inoculation tests, 20 isolates showed 100% and 4 isolates 60% relative virulence while 6 isolates were non-pathogenic. Moreover, 2 and 10 isolates were resistant to ciprofloxacin and cefoxitin, respectively, while the rest susceptible to other antibiotics used in this study. ERIC- and REP-PCR collectively depicted that the isolates from placental bit and vaginal swab had distinct PCR fingerprints except a few isolates with identical patterns. This study demonstrates prevalence of pathogenic strains mostly resistant to cefoxitin and/or ciprofloxacin. The results indicate the importance of isolating and characterizing the pathogen from human clinical samples as the pre-requisite for accurate epidemiological investigations. Listeria monocytogenes, a life-threatening pathogen, poses severe risk during pregnancy, may cause abortion, fetal death or neonatal morbidity in terms of septicemia and meningitis. The present study aimed at characterizing L. monocytogenes isolated from pregnant women based on serotyping, antibiotic susceptibility, virulence genes, in vivo pathogenicity test and ERIC- and REP-PCR fingerprint analyses. The results revealed that out of 3700 human clinical samples, a total of 30 (0.81%) isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from vaginal swab (2200)] were positive for L. monocytogenes. All the isolates belonged to serogroup 4b, and were + ve for virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA, hlyA, and iap. Based on the mice inoculation tests, 20 isolates showed 100% and 4 isolates 60% relative virulence while 6 isolates were non-pathogenic. Moreover, 2 and 10 isolates were resistant to ciprofloxacin and cefoxitin, respectively, while the rest susceptible to other antibiotics used in this study. ERIC- and REP-PCR collectively depicted that the isolates from placental bit and vaginal swab had distinct PCR fingerprints except a few isolates with identical patterns. This study demonstrates prevalence of pathogenic strains mostly resistant to cefoxitin and/or ciprofloxacin. The results indicate the importance of isolating and characterizing the pathogen from human clinical samples as the pre-requisite for accurate epidemiological investigations.
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