Moving Rate of Positive Patient Results as a Quality Control Tool for High-Sensitivity Cardiac Troponin T Assays

Li Tingting,Cao Shunwang,Wang Yi,Xiong Yujuan,He Yuting,Ke Peifeng,Huang Xianzhang 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.1

Background: A small shift in high-sensitivity cardiac troponin T (hs-cTnT) assays can lead to different result interpretation and consequent patient management. We explored whether a small bias could be detected using conventional internal quality control (QC) procedures, evaluated the performance of moving average (MA)-based QC procedures, and proposed a new QC procedure based on the moving rate (MR) of positive patient results of hs-cTnT assays. Methods:The ability of conventional QC to detect a 5 ng/L bias was examined using the13s/ 22s/R4s multi-rule procedure as deviation rules.We developed MA and MR procedures for the hs-cTnT assay using eight months of patient data. The performance of different MA or MR procedures was investigated by calculating the median number of patient samples affected until a bias introduced into the dataset was detected (MNPed). After comparing the MNPed across different procedures, we selected an optimal MA or MR procedure for validation. Validation graphs were plotted using the minimum, median, and maximum number of results affected until bias detection. Results: Our conventional QC procedures could not detect a positive bias of 5 ng/L. When a positive bias was introduced, MNPed was much higher using MA than using MR, with cut-off values of 5 ng/L and 14 ng/L, respectively. MR validation charts for optimal procedures provided insight into the MR performance. Conclusions: The MR procedure could detect different errors with few false alarms. In the hs-cTnT assay, the MR procedure with a smaller cut-off value outperformed MA and conventional QC procedures for small bias detection.

The effect of crash pulse on occupant injury in frontal coach collisions

Sun Tingting,Xiong Xin,Zheng Zhuan,Shi Xiaolong,Fan Jianwei 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.11

The purpose of this study is to reveal the effect of different acceleration curves on occupant injury risk in the frontal collision of coaches. Based on characteristics of frontal crash acceleration curves, the influence of characteristic parameters on occupant injury was studied using a two-factor variance analysis method. The finite element-rigid body coupling method was used to establish a coupled simulation for the dummy and the sled and evaluate differences in the effects on occupant injury between the acceleration curves of four different coaches and the acceleration envelope of the ECE R80 standard. Results indicate that the upper limit of the simplified curve has the greatest influence on occupant injury and it displays a positive correlation. The acceleration curve stipulated by ECE R80 is too idealized, which may lead to inadequacies in safety protection capability in vehicle and seat design.

Strong adsorption of cationic dyes by cucurbit[7]uril modified magnetic carbon nanotubes: Investigation on adsorption performance, reusability, and adsorption mechanism

Yujie Hu,Changjun Zou,Tingting Xiong,Huihui Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

In this work, a facile one-pot method was used to synthesize the co-modified carbon nanotube adsorbent(CB/MMCNT) of cucurbit[7]uril and Fe3O4. A series of characterizations were utilized to confirm the successfulsynthesis of the adsorbent. VSM and separation experiments demonstrated that the adsorbentexhibited excellent magnetic separation performance and could achieve rapid separation within 30 s. BET results show that the adsorbent has excellent specific surface area (67.438 m2/g). Subsequently, threecationic dyes, namely Methylene blue (MB), Malachite green (MG), and Gentian violet (GV), were utilizedin batch adsorption experiments. A strong adsorption effect on cations is exerted by the adsorbentthrough hydrogen bonds, electrostatic interactions, p-p conjugation effects and hydrophobic interactions. Under the optimal conditions (pH = 6, T = 300 min, C0 = 1000 mg/L), the maximum adsorptioncapacity of CB/MMCNT to MB, MG, and GV was 851.5 mg/g, 720.2 mg/g, and 563.4 mg/g. The adsorptiondata could be well fitted by both the pseudo-second-order kinetic model (R2 > 0.99) and Langmuir isothermmodel (R2 > 0.99). Thermodynamic data indicated that the adsorption process was spontaneousand endothermic. Moreover, the cyclic reuse performance of CB/MMCNT was evaluated through 7 cyclesof recycling experiments, which demonstrated that it maintained a high dye removal rate (MB 84%, MG78%, GV 81%), indicating its excellent reusability. Additionally, CB/MMCNT still maintained its impressivecationic dye removal performance (MB 95.8%, MG 92.7%, GV 92.2%) when tested in practical water environments. Overall, CB/MMCNT is a promising, environmentally friendly, efficient, and sustainable adsorbentfor removing cationic dyes.

Research on Influencing Factors of Chinese Mobile Communication Customers’ Switching Intention by Mobile Number Portability

Miao Miao,Jia Jia,Rui Tingting,Xiong Fangping,Li haibo 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.4

In order to explore the Influencing Factors of Switching Intention by Mobile Number Portability .We build up a structural equation model for Chinese mobile customers’ influence factors on MNP intention. Including competitive attraction, subjective norm, and policy perception, customer satisfaction, switching barriers, network quality, awareness rates, perceived service quality, usage habits and switching cost. According to empirical analysis, we find that customers with different perceive levels in MNP polices have significant differences in switching intention. And through the analysis and comparison, we find that the influence factors and the path coefficients of the customers’ switching intention are also different when they have different perceive levels in MNP policy.

Combining Non-Contrast CT Signs With Onset-to-Imaging Time to Predict the Evolution of Intracerebral Hemorrhage

Song Lei,Qiu Xiaoming,Zhang Cun,Zhou Hang,Guo Wenmin,Ye Yu,Wang Rujia,Xiong Hui,Zhang Ji,Tang Dongfang,Zou Liwei,Wang Longsheng,Yu Yongqiang,Guo Tingting 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.2

Objective: This study aimed to determine the predictive performance of non-contrast CT (NCCT) signs for hemorrhagic growth after intracerebral hemorrhage (ICH) when stratified by onset-to-imaging time (OIT). Materials and Methods: 1488 supratentorial ICH within 6 h of onset were consecutively recruited from six centers between January 2018 and August 2022. NCCT signs were classified according to density (hypodensities, swirl sign, black hole sign, blend sign, fluid level, and heterogeneous density) and shape (island sign, satellite sign, and irregular shape) features. Multivariable logistic regression was used to evaluate the association between NCCT signs and three types of hemorrhagic growth: hematoma expansion (HE), intraventricular hemorrhage growth (IVHG), and revised HE (RHE). The performance of the NCCT signs was evaluated using the positive predictive value (PPV) stratified by OIT. Results: Multivariable analysis showed that hypodensities were an independent predictor of HE (adjusted odds ratio [95% confidence interval] of 7.99 [4.87–13.40]), IVHG (3.64 [2.15–6.24]), and RHE (7.90 [4.93–12.90]). Similarly, OIT (for a 1-h increase) was an independent inverse predictor of HE (0.59 [0.52–0.66]), IVHG (0.72 [0.64–0.81]), and RHE (0.61 [0.54– 0.67]). Blend and island signs were independently associated with HE and RHE (10.60 [7.36–15.30] and 10.10 [7.10–14.60], respectively, for the blend sign and 2.75 [1.64–4.67] and 2.62 [1.60–4.30], respectively, for the island sign). Hypodensities demonstrated low PPVs of 0.41 (110/269) or lower for IVHG when stratified by OIT. When OIT was ≤ 2 h, the PPVs of hypodensities, blend sign, and island sign for RHE were 0.80 (215/269), 0.90 (142/157), and 0.83 (103/124), respectively. Conclusion: Hypodensities, blend sign, and island sign were the best NCCT predictors of RHE when OIT was ≤ 2 h. NCCT signs may assist in earlier recognition of the risk of hemorrhagic growth and guide early intervention to prevent neurological deterioration resulting from hemorrhagic growth.

Modeling and Virtual Screening of Antisense Peptides Targeting the Divergent Region of Tumor-Associated MT1-MMP Protein

Bowen Tan,Yijie Zhou,Zhilei Song,Yinxuan Peng,Fang Wu,Yue Kang,Xiaomin Liu,Li Zeng,Tingting Huang,Zongying Liu,Lili Xiong,Zhiyun Guo,Jian Cui,Canquan Mao 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.9

Membrane type-1 matrix metalloproteinase (MT1-MMP; also known as MMP14) is a key enzyme involved in tumor invasion and metastasis, and is a potential target for drug discovery for cancer therapy. However, till now there is no MT1-MMP- or MMP-based anticancer drugs in the market mainly because of the high conservation of the MMP family and also because there is no elucidated crystal structure for the mature MT1-MMP. The modeling of the three-dimensional structure of mature MT1-MMP and the finding of MT1-MMP targeted peptides by virtual screening are highly desired. In this study, the three-dimensional structure of mature MT1-MMP is constructed by homology and de novo modeling and later rationalized and optimized by molecular dynamics simulations. An antisense peptide library was constructed against the divergent sense peptide DEGTEEET in the specific region of MT1-MMP, which was found by multiple alignment of the whole MMP family. The antisense peptide library was virtually screened against the constructed three-dimensional model of MT1-MMP. The top 20 novel peptides were further studied, which were found well docked with MT1-MMP at the region of DEGTEEET, again confirming their specific binding to MT1-MMP. Preliminary study of one of the top-ranked peptide SFLLSPFV showed that it could inhibit the viabilities of MG63 and MDA-MB-231 tumor cells. We thus not only successfully modeled the three-dimensional structure of mature MT1-MMP but also provided a new way for the finding of peptide candidates targeting MT1-MMP based on antisense peptide library.