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Yu Shun Tian,Hyun Joo Jeong,Sang-Do Lee,Seok Heui Kong,Seung-Ho Ohk,Yun-Jung Yoo,Jeong-Taeg Seo,Dong Min Shin,Byung-Wha Sohn,Syng-Ill Lee 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3
The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1Ձ, 25-dihydroxyvitamin D<sub>3</sub> (1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>- induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ՊB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>. Knockdown of Runx2 inhibited 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1Ձ,25(OH)<sub>2</sub>D<sub>3</sub>- induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.
Tian, Yu-Shun,Jeong, Hyun-Joo,Lee, Sang-Do,Kong, Seok-Heui,Ohk, Seung-Ho,Yoo, Yun-Jung,Seo, Jeong-Taeg,Shin, Dong-Min,Sohn, Byung-Wha,Lee, Syng-Ill The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3
The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects $1{\alpha}$, 25-dihydroxyvitamin $D_3$ ($1{\alpha},25(OH)_2D_3$)-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of $1{\alpha},25(OH)_2D_3$-induced tartrate-resistant acid phosphatase-positive multinucleated cells and $1{\alpha},25(OH)_2D_3$-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) and Runx2 expressions were down-regulated in response to $1{\alpha},25(OH)_2D_3$. Knockdown of Runx2 inhibited $1{\alpha},25(OH)_2D_3$-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of $1{\alpha},25(OH)_2D_3$-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells.
A Two-Photon Tracer for Glucose Uptake
Tian, Yu Shun,Lee, Hyang Yeon,Lim, Chang Su,Park, Jongmin,Kim, Hwan Myung,Shin, Yoo Na,Kim, Eun Sun,Jeon, Hoon Jae,Park, Seung Bum,Cho, Bong Rae WILEY-VCH Verlag 2009 Angewandte Chemie. international edition Vol.48 No.43
<B>Graphic Abstract</B> <P>It takes two: Two-photon tracers (AG1, AG2) have been developed that can be excited by 780 nm fs laser pulses, can be easily loaded into cancer cells and tissues, show high photostability and negligible toxicity, and can visualize glucose uptake in living cells and tissues by two-photon microscopy (TPM). Moreover, AG2 can screen anticancer agents in cancer cells and cancer tissues at 75–150 mm depth by TPM. <img src='wiley_img/14337851-2009-48-43-ANIE200901175-content.gif' alt='wiley_img/14337851-2009-48-43-ANIE200901175-content'> </P>
Detection of Near-membrane Calcium Ions in Live Tissues with a Two-Photon Fluorescent Probe
Yu Na Shin,임창수,Yu Shun Tian,Won Young Rho,조봉래 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.3
A two-photon fluorescent probe (ACaCL) that can detect near-membrane Ca2+ is reported. This probe can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane Ca2+ in live cells and deep inside live tissues by two-photon microscopy.
Wang Tian-Yu,Li Xiao-Fen,Liu Shu-Ming,Liu Bai-Xin,Liang Xi-Dong,Li Shunning,Zhang Gui-Xin,Liu Jian-Bo,Dang Zhi-Min 나노기술연구협의회 2022 Nano Convergence Vol.9 No.55
Polymer dielectrics are insulators or energy storage materials widely used in electrical and electronic devices. Polymer dielectrics are needed with outstanding dielectric characteristics than current technologies. In this study, the self-assembly of boron nitride nanosheets (BNNSs) was applied to form an inorganic–organic nanocoating on various common polymer dielectrics. It is inexpensive and easy to fabricate this thin coating on a large scale. The coating has a wide bandgap and thus can significantly improve the breakdown strength of polymer dielectrics. The charge characteristics and trapping parameters of nano-domains on the surfaces of polymer dielectrics were measured, and the coating had shallow trap levels. This facilitated the dissipation of surface charges and thus greatly increased the flashover voltage. The coating also effectively improved the temperature stability and dielectric constant of the polymer dielectric. This nanocoating shows potential as a method to effectively improve the dielectric characteristics of polymer dielectrics and outperform existing composite polymer dielectrics, which are crucial for large-scale applications in energy storage and power and electronic devices.
Detection of Near-membrane Calcium Ions in Live Tissues with a Two-Photon Fluorescent Probe
Shin, Yu-Na,Lim, Chang-Su,Tian, Yu Shun,Rho, Won-Young,Cho, Bong-Rae Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.3
A two-photon fluorescent probe (ACaCL) that can detect near-membrane $Ca^{2+}$ is reported. This probe can be excited by 780 nm fs pulses, shows high photostability and negligible toxicity, and can visualize near-membrane $Ca^{2+}$ in live cells and deep inside live tissues by two-photon microscopy.
CDK2 is Required for the DNA Damage Response during Porcine Early Embryonic Development
HaiYang Wang,Yu-Jin Jo,Tian-Yi Sun,Suk Namgoong,Xiang-Shun Cui,Jeong Su Oh,Nam-Hyung Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
CDK2 inhibition plays a central role in DNA damage–induced cell cycle arrest and DNA repair. However, whether CDK2 also influences early porcine embryo development is unknown. In this study, we examined whether CDK2 is involved in the regulation of oocyte meiosis and early embryonic development of porcine. We found that disrupting CDK2 activity with RNAi or an inhibitor did not affect meiotic resumption or MII arrest. However, CDK2 inhibitor-treated embryos showed delayed cleavage and ceased development before the blastocyst stage. Disrupting CDK2 activity is able to induce sustained DNA damage as demonstrated by the formation of distinct γH2AX foci in nuclei of day 3- and day 5-embryos. Inhibiting CDK2 triggers a DNA damage checkpoint by activating of the ATM-P53-P21 pathway. However, the mRNA expression of genes involved in non-homologous end-joining (NHEJ) or homologous recombination (HR) pathways for double strand break (DSB) repair reduced after administering CDK2 inhibitor to 5-day-old embryos. Furthermore, CDK2 inhibition caused apoptosis in day 7 blastocysts. Thus, our results indicate that an ATM-P53-P21 DNA damage checkpoint is intact in the absence of CDK2; however, CDK2 is important for proper repair of the damaged DNA by either directly or indirectly influencing DNA repair-related gene expression.