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이유자돈에 있어서 복합 생균제(MR-1)의 사료 내 첨가가 성장 능력 및 생화학적 조성, 면역 반응에 미치는 영향
이상범(Sang-Bum Lee),이재성(Jae-Sung Lee),왕도(Tao Wang),김민정(Min-Jeong Kim),정우석(Woo-Suk Jung),전승우(Seung-Woo Jeon),박윤정(Yun-Jeong Park),신택순(Teak-Soon Shin),박상홍(Sang-Hong Park),이홍구(Hong-Gu Lee) 한국생명과학회 2014 생명과학회지 Vol.24 No.4
이 연구의 목적은 이유자돈에 있어서 목장토에서 자체 분리 동정한 19종의 혼합 미생물 제제인 MR-1이 in vitro 실험(축 종별 병원성 미생물에 대한 항균효과 검정)과 in vivo 실험(성장, 혈액 생화학, 면역력 검정)에 미치는 영향을 평가하고자 하였다. 38일령 이유자돈 69두를 처리구 당 23두씩 나누어 대조구(basal diet; No-Antibiotics), 항생 제구(Antibiotics), MR-1구(No-Antibiotics + MR-1)로 배치하여 45일 동안 급여시켰다. MR-1에 대한 축 종별 병원성 미생물에 대한 항균 효과 결과 E. coli K88와 S. Typhimurium균에서 약한 항균효과(+)를 나타내었으며, E. coli K99균에 대해서는 아주 강한 항균효과(++++)를 나타내었다. MR-1에 대한 일 당 증체량, 일 당 사료섭취량 및 사료요구율 결과는 MR-1의 사료 내 첨가에 의해 일 당 증체량은 증가 30 g 증가된 반면, 일 당 사료 섭취량은 감소하여 결과적으로 사료요구율이 대조구에 비해 유의하게 개선된 것으로 나타났다(p<0.05). 혈액 생화학적 분석의 결과는 WBC (백혈구)농도가 다른 처리구보다 높게 변화하였으며, MCH (평균 혈구 헤모글로빈 량) 농도 및 MCHC (평균 혈구 헤모글로빈 농도)는 각각 항생제구와 대조구보다 유의하게 높아졌다(p<0.05). 혈장 내 cytokine production의 농도 결과 MR-1의 사료 내 첨가에 의해 TNFα의 경우 처리간 변화가 없었으나, IFNγ의 경우 항생제구에서 다른 처리구보다 유의하게 낮아진 반면, MR-1의 경우 대조구와 동일하게 유지된 결과를 나타내었다. 따라서 이유자돈에 있어서 혼합 미생물 제제인 MR-1은 병원성 미생물에 대한 항균력이 있을 뿐 만 아니라 사료 내 첨가 급여 시 생산성에서 효과가 유의하게 나타났으며, 항생제에 따른 바이러스 저항 인자인 IFNγ의 감소 없이 유지되는 것으로 나타나 이유자돈에 있어서 항생제 대체제로서 산업적으로 이용이 적합할 것으로 판단된다. We evaluated the growth performance, biochemical characteristics, and immune responses in weaning pigs given a diet containing MR-1 (0.2%/feed) or antibiotics (0.1%/feed) for 45 days. In vitro study showed that MR-1 has antibacterial activity against a variety of strains of pathogenic bacteria, especially a strain of cattle-derived Escherichia coli K99 (E. coli K99) by agar diffusion assay. In the in vivo model, 0.2% MR-1-given group clearly ameliorated the weight gain and feed efficiency in the growth performance of weaning pigs compared to the basal diet group (p<0.05). Additionally, 0.2% MR-1 induced an elevation in the levels of mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) and showed a similar pattern (TNFα and IFNγ production) to the antibiotic treated pigs. Taken together, we suggest that 0.2% MR-1 makes probiotics an alternative to antibiotics in weaning pigs.
Sang Jun Yoon,Jae Goo Lee,Hideo Tajima,Akihiro Yamasaki,Tetsuya Nakazato,Hiroaki Tao,Fumio Kiyono 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.3
Extractions of five kinds of lanthanide metal ions by bis(2-ethylhexyl)phosphoric acid (DEHPA) with [1-Cn-3-methylimidazolium][PF6](Cn = C2, C4) or [1-butyl-4-methylpyridinium][PF6] were carried out under various DEHPA and HNO3 concentrations from 0 to 1 M and under different temperature conditions from 298 to 333 K. These results were compared with those using the conventional organic solvent, hexane, in terms of their distribution coefficient values. Under all of the conditions in this study,the ionic liquid system shows more than three times greater extractability for lanthanide compared to when hexane was used. The distribution coefficient of lanthanide ions decreased as the length of the alkyl chain increased from the ethyl to the butyl. In addition, the imidazolium cation generally shows a higher distribution coefficient compared to the pyridinium cation in an ionic liquid. The concentration ratio of lanthanides and DEHPA resulted in an extraction affinity transition for lanthanides. Also evaluated in this study were issues related to the selectivity associated with the lanthanide mixture and the dependency of the ionic radius during lanthanide extraction. 2010 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
( Sang-bum Lee ),( Kyung-won Lee ),( Tao Wang ),( Jae-sung Lee ),( U-suk Jung ),( Jalil Ghassemi Nejad ),( Young-kyoon Oh ),( Youl-chang Baek ),( Kyoung Hoon Kim ),( Hong-gu Lee ) 한국축산학회 2019 한국축산학회지 Vol.61 No.4
This study aimed to determine the effective dose of intravenous administration of L-tryptophan (L-T) on gastrointestinal hormones (GIH) secretions and melatonin using Hanwoo cattle. Three steers (362 ± 23 kg) fitted with indwelling jugular vein catheters were assigned in a 3 × 3 Latin square design. Treatments were intravenous administration of saline (control), 28.9 mg L-T/kg body weight (BW; low) and 57.8 mg L-T/kg BW (high) L-T for 1 day with 7 days of adaptation. Samples were collected after adaptation period at -60, 0, 30, 60, 90, 120, 150, 180, 240, and 300 min of sampling day. The levels of serum cholecystokinin (CCK) and secretin were higher (p < 0.05) in the high L-T group than those in the other groups. Serum Melatonin (MEL) levels were increased upon L-T administration (p < 0.05) in the high L-T group. Taken together, the effective dose of L-T administration was defined at 57.8 mg L-T/kg BW in order to stimulate increase of GIH and MEL.
Sung-ho Ha(하성호),Luo-tao Liu(류라도) 한국인터넷전자상거래학회 2009 인터넷전자상거래연구 Vol.9 No.4
인터넷의 발전으로 온라인 쇼핑은 한국에서 점점 대중화되고 있다. 특히, 소비자간 (C2C) 전자상거래가 점차 대중화되고 있다. 본 연구의 목적은 C2C 전자상거래에서 고객의 구매 의도에 관한 통합 모델을 제안하는 것이다. 선행연구에서 연구자들은 TAM 모형을 기반으로 지각된 웹사이트 품질이 온라인 비즈니스를 운영하는 중요한 요인이라는 것을 밝혔다. 본 연구는 구매의도가 지각된 웹사이트품질, 지각된 유용성, 제3자 인지, 만족과 신뢰에 영향을 받는다고 가설 설정하였다. 실증데이터는 구조방정식모델을 사용하여 분석하였으며, 지각된 웹사이트품질이 만족도에 미치는 영향과 지각된 유용성이 구매의도에 미치는 영향을 제외한 나머지 가설은 채택되어졌다.
BLDC Motor Control System using ASK DC-link PLC Technology
Tao Yu,Sung-Geun Song,Se-Min Kim,Kwang-Heon Kim,Sung-Jun Park 한국조명·전기설비학회 2009 조명·전기설비학회논문지 Vol.23 No.3
Power line communication or power line canier (PLC) is a system for carrying data on a conductor and can also used for electric power transmission. Now the powers used in the motor drive system are mostly DC sources. Even AC powers in some systems are generated from DC sources by the inverter. It is can be forecasted that the DC-link PLC technology will be widely used in various industrial fields. In this paper a novel BLDC motor control system using ASK DC-link PLC technology is proposed. The characteristic of this system is that the communication only needs 2 DC lines and there are no additional lines to translate the reference signals. The number of signal cables can be minimized when the DC-link PLC method is applied in the multi motor control system and the slip ring design also be simplified when this method is applied in rotation machines. The proposed motor control system is clarified by the PSIM and MATLAB simulations and tested through the hardware prototype.
Oral Delivery of Probiotics in Poultry Using pH-Sensitive Tablets
( Tao Jiang ),( Hui-shan Li ),( Geon Goo Han ),( Bijay Singh ),( Sang-kee Kang ),( Jin-duck Bok ),( Dae-duk Kim ),( Zhong-shan Hong ),( Yun-jaie Choi ),( Chong-su Cho ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4
As alternatives to antibiotics in livestocks, probiotics have been used, although most of them in the form of liquid or semisolid formulations, which show low cell viability after oral administration. Therefore, suitable dry dosage forms should be developed for livestocks to protect probiotics against the low pH in the stomach such that the products have higher probiotics survivability. Here, in order to develop a dry dosage forms of probiotics for poultry, we used hydroxypropyl methylcellulose phthalate 55 (HPMCP 55) as a tablet-forming matrix to develop probiotics in a tablet form for poultry. Here, we made three different kinds of probiotics-loaded tablet under different compression forces and investigated their characteristics based on their survivability, morphology, disintegration time, and kinetics in simulated gastrointestinal fluid. The results indicated that the probiotics formulated in the tablets displayed higher survival rates in acidic gastric conditions than probiotics in solution. Rapid release of the probiotics from the tablets occurred in simulated intestinal fluid because of fast swelling of the tablets in neutral pH. As a matrix of tablet, HPMCP 55 provided good viability of probiotics after 6 months under refrigeration. Moreover, after oral administration of probiotics-loaded tablets to chicken, more viable probiotics were observed, than with solution type, through several digestive areas of chicken by the tablets.
A Novel Ripple-Reduced DC-DC Converter
Tao, Yu,Park, Sung-Jun The Korean Institute of Power Electronics 2009 JOURNAL OF POWER ELECTRONICS Vol.9 No.3
A DC/DC converter generally needs to work under high switching frequency when used as an adjustable power supply to reduce the size of magnetic elements such as inductors, transformers and capacitors, but with the rising of the switch frequency, the switch losses will increase and the efficiency will reduce. Recently, to solve these problems, research is actively being done on a soft switching method that can be applied under high frequency and on a PWM converter that can be applied under low frequency such as a multi-level topology. In this paper a novel DC-DC conversion method for reducing the ripple of output voltage is proposed. In the proposed converter, buck converters are connected in series to generate the output voltage. By using this method, the ripple of output voltage can be reduced compared to a conventional buck converter. Particularly when output voltage is low, the number of acting switching elements is less and the result of ripple reduction is more obvious. It is expected that the converter proposed in this paper could be very useful in the case of wide-range output voltage.