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      • Importance of sandy bottoms in coral reefs to the oscillation of daily rhythms in the tropical wrasse <i>Halichoeres trimaculatus</i>

        Ito-Takeuchi, Hitomi,Takahashi, Kai,Bouchekioua, Selma,Yamauchi, Chihiro,Takeuchi, Yuki,Hur, Sung-Pyo,Lee, Young-Don,Takemura, Akihiro Informa UK (TaylorFrancis) 2017 Chronobiology international Vol.34 No.8

        <P>Most wrasse species swim during the day and bury themselves in the sandy bottoms of shallow reefs at night. This study aimed to evaluate the importance of sandy bottoms to the day-active/ night-inactive rhythmicity of the tropical wrasse Halichoeres trimaculatus. Actogram analysis revealed that fish were active during the photophase and inactive during the scotophase in aquariums with both sandy and bare bottoms. When fish were kept in aquariums with bare bottoms, rhythmicity was maintained under constant dark conditions (DD) but became obscured under constant light conditions (LL), suggesting that a day-active/night-inactive rhythmicity is regulated by the circadian system. Robust fluctuations in Period1 (wPer1) and Period2 (wPer2) expression were observed in the pectoral fin tissue under light-dark conditions (LD). Similar fluctuations in wPer1 expression persisted under DD. When fish were kept under LD conditions for 7 days and then DD for 20 days, the emergence of fish from the sandy bottom was delayed gradually. At the same time, the peak time of wPer1 expression under DD was retarded from 06: 00 to 10:00. Although wPer2 expression was dampened under DD, it increased after exposing fish to light. These results suggest that wPer1 and wPer2 are differentially involved in the day-active/ night-inactive rhythmicity, and that blocking light with a sandy bed at night and exposing fish to light during emergence in the morning play important roles in maintaining consistent activities in wrasse species.</P>

      • SCIESCOPUSKCI등재

        Periodontal wound healing following reciprocal autologous root transplantation in class III furcation defects

        Takeuchi, Naoshi,Shirakata, Yoshinori,Shinohara, Yukiya,Sena, Kotaro,Noguchi, Kazuyuki Korean Academy of Periodontology 2017 Journal of Periodontal & Implant Science Vol.47 No.6

        Purpose: Furcation involvement in the molars is difficult to treat, and has been recognized as a risk factor for tooth loss. Although periodontal regenerative therapies, including guided tissue regeneration and various types of bone grafts, have been applied to furcation defects, the effects of these treatments are limited, especially in large class III furcation defects. The purpose of this pilot study was to investigate the effect of reciprocal autologous root transplantation on periodontal wound healing and regeneration in class III furcation defects in dogs. Methods: Furcation defects (7 mm wide and 6 mm high) were surgically created after root separation of the unilateral third and fourth premolars in 4 dogs. Eight furcation defects were randomized to receive either reciprocal autologous root transplantation (test) or no further treatment (control). In the test group, the mesial and distal roots were transplanted into the distal and mesial extraction sockets, respectively. The animals were sacrificed 10 weeks after surgery for histologic evaluation. Results: The healing pattern in the control group was characterized by extensive collapse of the flap and limited periodontal regeneration. New bone formation in the test group ($3.56{\pm}0.57mm$) was significantly greater than in the control group ($0.62{\pm}0.21mm$). Dense collagen fibers inserting into the residual cementum on the transplanted root surfaces were observed in the test group. Slight ankylosis was observed in 2 of the 4 specimens in the test group on the mesiodistal sides where the root-planed surfaces faced the existing bone. Root resorption (RR) was detected in both the control and test groups. Conclusions: Within the limits of this study, it can be concluded that reciprocal autologous root transplantation was effective for bone regeneration in class III furcation defects in dogs. However, further studies are required to standardize the approach in order to prevent unwanted RR prior to clinical application.

      • Oxidative stress on anaerobes

        Takeuchi, Toru,Shi, Minyi,Kato, Naoki,Watanabe, Kunitomo,Morimoto, Kanehisa Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

        A strict anaerobe, Prevotella melaninogenica is highly sensitive to oxidative stress. Oxidative stress such as exposure to oxygen or addition of hydrogen peroxide, increased 8-hydroxydeoxyguanosine (80HdG), a typical of oxidative DNA damage, and decreased the bacterial cell survival rate. We could detect the generation of reactive oxygen species in P. melaninogenica after exposure to oxygen. UVA irradiation also increased 80HdG in the bacterium. On the other hand, such oxidative stress did not increase 80HdG in a facultative anaerobe. These findings suggest that P. melaninogenica is a suitable material to study the biological effects of oxidative stress, to evaluate antioxidants, and to study the effects of oxygen or reactive oxygen species on molecular evolution.

      • SCOPUSSCIEKCI등재

        IgG4-Related Intracranial Hypertrophic Pachymeningitis : A Case Report and Review of the Literature

        Takeuchi, Satoru,Osada, Hideo,Seno, Soichiro,Nawashiro, Hiroshi The Korean Neurosurgical Society 2014 Journal of Korean neurosurgical society Vol.55 No.5

        Hypertrophic pachymeningitis is an uncommon disorder that causes a localized or diffuse thickening of the dura mater. Recently, the possibility that IgG4-related sclerosing disease may underlie some cases of intracranial hypertrophic pachymeningitis has been suggested. We herein report the tenth case of IgG4-related intracranial hypertrophic pachymeningitis and review the previous literature. A 45-year-old male presented with left-sided focal seizures with generalization. Magnetic resonance imaging (MRI) revealed a diffuse thickening and enhancement of the right convexity dura matter and falx with focal nodularity. The surgically resected specimens exhibited the proliferation of fibroblast-like spindle cells and an infiltration of mononuclear cells, including predominantly plasma cells. The ratio of IgG4-positive plasma cells to the overall IgG-positive cells was 45% in the area containing the highest infiltration of plasma cells. On the basis of the above findings, IgG4-related sclerosing disease arising from the dura mater was suspected. IgG4-related sclerosing disease should be added to the pachymeningitis spectrum.

      • KCI등재

        Comparison of the Fixation Strengths of Screws between the Traditional Trajectory and the Single and Double Endplate Penetrating Screw Trajectories Using Osteoporotic Vertebral Body Models Based on the Finite Element Method

        Takeuchi Takumi,Takamizawa Yuhei,Konishi Kazumasa,Sano Hideto,Takahashi Masahito,Kouno Hitoshi,Hosogane Naobumi 대한척추외과학회 2024 Asian Spine Journal Vol.18 No.1

        Study Design: This is a finite element (FE) study. Purpose: To compare the fixation strength of traditional trajectory (TT) and single and double endplate penetrating screw trajectories (SEPST/DEPST) to the osteoporotic vertebral body model based on the FE method. Overview of Literature: SEPST/DEPST have been developed to enhance the fixation strength in patients with diffuse idiopathic hyperostosis (DISH). This technique was also applied to patients with osteoporosis. However, determining the superiority of SEPST/DEPST is difficult because of the heterogeneous patient backgrounds. Methods: Twenty vertebrae (T12 and L1) from 10 patients with osteoporosis (two males and eight females; mean age, 74.7 years) were obtained to create the 10 FE models. First, a single screw was placed with TT and SEPST/DEPST, and the fixation strength was compared by axial pullout strength (POS) and multidirectional loading tests. Second, two screws were placed on the bilateral pedicles with TT and SEPST/DEPST, and the fixation force of the vertebrae in the constructs in flexion, extension, lateral flexion, and axial rotation was examined. Results: SEPST and DEPST had 140% and 171% higher POS values than TT, respectively, and the DEPST result was statistically significant (p=0.007). The multidirectional fixation strength was significantly higher in DEPST and SEPST than in TT in the cranial, caudal, and medial directions (p<0.05) but not in the lateral direction (p=0.05). The vertebral fracture strength at the lower instrumented vertebra of the DEPST tended to be higher than that of TT. The vertebral motion angles in SEPST and DEPST were significantly smaller in lateral bending (p=0.02) and tended to be smaller in flexion and extension than in TT (p=0.13). Conclusions: This study may provide useful information for spine surgeons in deciding whether to choose the SEPS or DEPS technique for augmenting fixation in osteoporotic vertebral fracture surgery.

      • KCI등재

        地域をメンテナンスする考え方を用いた 「まちづくり」のあり方についての考察

        ( Takeuchi Yuji ) 한국일본근대학회 2018 일본근대학연구 Vol.0 No.59

        本稿の目的は、「地域をメンテナンスするという活動とは、どのような活動なのか」を意義付けすることである。この点を明らかにする理由として、實際の活動を前提に考えれば、實務者は活動そのものの全體像としての行動がわからなければ不安で仕方がないからである。だからこそ、活動の方向性を示すことができなければ、現場に適應·對應させることすら難しい。その問題点として、(1)現在までに確認されている、まちづくりに關する數多くの槪念が發展途中であり、今後の社會動向に委ねるものや問題提起で終わっているものなど、狀況が樣·であること、(2)實社會での地域經營では、市民セクタ一の補强が必要であることの2点が上げられた。しかし、問題点を浮き彫りにしただけで、今後の方向性や解決策としての補强方法などを明らかにしていない。 だからこそ、地域をメンテナンスする活動とは何かを明らかにする必要がある。その方法としては、まちづくりに關する旣往硏究から「まちづくり」そのものの性格や性質について確認する。その上で、地域メンテナンス活動の全體像を明らかにした上で、實社會における地域經營に關する問題点を重ね合わせることで、地域メンテナンスとは、どのような活動なのかを示す。 The purpose of this paper is to clarify “what kind of activities are activities to maintain the region” as a definition. The reason for clarifying this point is that if an actual worker is assumed on the premise, the practitioner is uneasy and can’t help without knowing the action as the overall picture of the activity itself. That is why even if it is not possible to show the direction of the activity, it is difficult even to adapt and correspond to the site. As a problem, (1) numerous concepts concerning “city planning” confirmed so far are being developed. There are also various situations such as those that leave it to social trends in the future and those that end with problem raising. (2) Regional management in the real world requires reinforcement of the civil sectors. These two points were raised. However, merely highlighting the problems, we do not disclose future directions and reinforcement methods as solutions. That is why it is necessary to clarify what activities are undertaken to maintain the area. As a way to do this, we confirm the nature and nature of “town development itself” from past research on town planning. Then, after clarifying the overall picture of regional maintenance activities, we superimpose problems on regional management in real society. By doing this, we will show what kind of activity is regional maintenance.

      • Effects of supplementary UV-B radiation on growth and protein biosyntheses in rice (Oryza sativa L.)

        Takeuchi, Atsuko,Hidema, Jun,Kumagai, Tadashi Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

        We examined the effects of supplementary ultraviolet-B (UV-B) radiation on the changes in synthesis and degradation of ribulose-I, 5-biphosphate carboxylase /oxygenase (Rubisco) and light-harvesting chlorophyll a/b binding protein of PSII (LHCII), as well as mRNA levels for small and large subunits of Rubisco (rbcS and rbcL, respectively) and LHCII (cab) with leaf age in UV-sensitive rice (Norin I) and UV-resistant rice (Sasanishiki). Both Rubisco and LHCII were actively synthesized until the leaf had fully expanded, and then decreased with leaf age. Synthesis of Rubisco, but not LHCII, was significantly suppressed by UV-B in Norin 1. The degradation of Rubisco was enhanced by UV-B around the time of the leaf maturation in the two cultivars. The levels of rbcS and rbcL were reduced by UV-B at the early leaf stages after emergence in both cultivars. The level of cab was first present at the highest level in the two cultivars, but drastically decreased due to UV-B treatment immediately after leaf emergence in Norin 1. It was proved that synthesis and degradation of Rubisco and LHCII greatly changed with leaf age: Rubisco synthesis was significantly suppressed by supplementary UV-B radiation at the transcription step during the early leaf stages. It was also suggested that the difference between the two rice cultivars in sensitivity to UV-B in the synthesis of Rubisco might be due to the specific suppression not only after transcription but also at transcription.

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