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Sechman, A.,Shimada, K.,Saito, N.,Ieda, T.,Ono, T. Asian Australasian Association of Animal Productio 1996 Animal Bioscience Vol.9 No.1
The present study was conducted to investigate the role of thyroid hormones in the regulation of gene expression of calbindin-$D_{28k}$ (CaBP-D28K) in the chicken. By employing slot blot and RIA analyses, levels of CABP-D28K mRNA and CaBP-D28K protein in the intestine, kidney, cerebellum and liver were measured 6 and 12 h after i.m. injection of 1, 25-dihydroxyvitamin $D_3$ [1, 25 $(OH)_2D_3$; 250 ng/chick] and 3, 5, 3'-triiodothyronine ($T_3$; 500 ng/chick) in one-day-old chicks. The abundant messages of CaBP-D28K mRNA were detected in the intestine, kidney and cerebellum while there was little message in the liver. After 1, 25 $(OH)_2D_3$ treatment (6 + 12 hours), levels of CaBP-D28K mRNA increased in the intestine, but there was no change in the mRNA levels in the kidney and cerebellum. Although $T_3$ alone had no effect on CaBP-D28K mRNA levels, simultaneous administration of $T_3$ enhanced the 1, 25 $(OH)_2D_3$ effect of levels of CaBP-D28K mRNA in the intestine both 6 and 12 h post-treatment, and in the kidney 12 h post-treatment. At a protein level, co-treatment with 1, 25 $(OH)_2D_3$ and $T_3$ elicited a significant increase in CaBP-D28K expression in the intestine 12 h post-treatment, as compared to treatment with only 1, 25 $(OH)_2D_3$, whereas no differences were observed in the CaBP-D28K protein levels in the kidney and cerebellum. These results suggest that thyroid hormones may play a synergistic role with 1, 25 $(OH)_2D_3$ for CaBP-D28K gene expression in the intestine and kidney in chicks.
Development of complete Culture System for Quail Embryos and Its Application for Embryo Manipulation
Ono, T. The Korean Society of Poultry Science 2001 韓國家禽學會誌 Vol.28 No.2
Gene and cell transfer technique will serve as a powerful tool for the genetic improvement of the poultry and to yield useful products. For avian transgenesis, Japanese quail may serve as an excellent animal model because of its small body size and fast growth rate. Recent progress was described on the manipulation of quail embryos such as the introduction of foreign genes and cells, and the subsequent culturing of the manipulated embryos yielding hatchlings. Intraspecific donor-derived offspring have been available in quail, however, further investigation will be required to obtain interspecific offspring with the aim of rescuing endangered species. Trans genesis will also be useful for improving the profitability and quality of poultry stocks and for developing stocks with novel uses. Considerable progress should soon be made toward the production of transgenic poultry. The key feature of the procedure described here is that embryos are initially taken out from the shell for ease of manipulation and then placed back in culture in addition to various operations midway during culture.
Kobayashi, T.,Ida, K.,Inagaki, S.,Tsuchiya, H.,Tamura, N.,Choe, G.H.,Yun, G.S.,Park, H.K.,Ko, W.H.,Evans, T.E.,Austin, M.E.,Shafer, M.W.,Ono, M.,Ló,pez-bruna, D.,Ochando, M.A.,Estrada, T.,Hidalg IOP 2017 Nuclear fusion. Fusion nucléaire. &n.Illiga Vol.57 No.7
<P>In this contribution we analyze modulation electron cyclotron resonance heating (MECH) experiment and discuss higher harmonic frequency dependence of transport coefficients. We use the bidirectional heat pulse propagation method, in which both inward propagating heat pulse and outward propagating heat pulse are analyzed at a radial range, in order to distinguish frequency dependence of transport coefficients due to hysteresis from that due to other reasons, such as radially dependent transport coefficients, a finite damping term, or boundary effects. The method is applied to MECH experiments performed in various helical and tokamak devices, i.e. Large Helical Device (LHD), TJ-II, Korea Superconducting Tokamak Advanced Research (KSTAR), and Doublet III-D (DIII-D) with different plasma conditions. The frequency dependence of transport coefficients are clearly observed, showing a possibility of existence of transport hysteresis in flux-gradient relation.</P>
Kobayashi, T,Kim, J H,Lee, S R,Araki, H,Ono, T IEEE 2010 IEEE geoscience and remote sensing letters Vol.7 No.3
<P>Simultaneous observations of Lunar Radar Sounder (LRS) and Laser ALTimeter (LALT) of Kaguya, a Japanese lunar exploration project, were carried out for the purpose of mapping regolith layer thickness of the Moon. Nadir surface echo of a high-frequency (5 MHz) pulse of LRS interferes the shallow (<; 10 m) subsurface echo from the bottom of the regolith layer, which subsequently makes the apparent surface be detected at a range deviated from the actual surface range, while the actual surface range is optically detected by LALT. Regolith layer thickness information is retrieved from this range difference after an inversion process. So far, four major maria on the near side of the Moon (Maria Tranquillitatis, Serenitatis, Imbrium, and Oceanus Procellarum) have been investigated, and the mean regolith layer thicknesses of the four maria were found to be about the same, ranging from 6.3 to 6.9 m. However, spatial distribution of areal regolith thickness appears different in eastern maria from western maria, which implies a difference of the growth history of the regolith layer.</P>