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Shahid S. Siddiqui,Ken Nishikawa,M.A.Shakir,M. Yusuf Ali,K.Fukami Kobayashi,M.L.A.Khan The Korea Science and Technology Center 2000 BMB Reports Vol.33 No.2
In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditis elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The in situ RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C-terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.
Ali, M. Yusuf,Khan, M.L.A.,Shakir, M.A.,Kobayashi, K. Fukami,Nishikawa, Ken,Siddiqui, Shahid S. 생화학분자생물학회 2000 Journal of biochemistry and molecular biology Vol.33 No.2
In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditis elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The insitu RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C-terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.
Domain Wall Pinning by a Stray Field from NiFe on a Co/Ni Nanowire
R. Hiramatsu,T. Koyama,H. Hata,T. Ono,D. Chiba,S. Fukami,N. Ishiwata 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.3
We have investigated the influence of a stray field on a magnetic domain wall (DW). Thedepinning fields of a DW in a perpendicularly magnetized Co/Ni nanowire with partly-stackedNi81Fe19/SiO2 islands on it was measured. The depinning field for the wire with Ni81Fe19/SiO2was found to be higher than that for the wire without the stack, indicating that the stray field fromthe Ni81Fe19/SiO2 stack clearly served as an artificial pinning site for the DW. The magnetic forcemicroscopy observation evidenced that the DW was pinned near the Ni81Fe19 stack.
Ali, M . Yusuf,Khan, M . L . A .,Shakir, M . A .,Kobayashi, K . Fukami,Nishikawa, Ken,Siddiqui, Shahid S . 생화학분자생물학회 2001 BMB Reports Vol.33 No.2
In eukaryotes, chromosomes undergo a series of complex and coordinated movements during cell division. The kinesin motor proteins, such as the chicken Chromokinesin, are known to bind DNA and transport chromosomes on spindle microtubles. We previously cloned a family of retrograde C-terminus kinesins in Caenorhabditts elegans that mediate chromosomal movement during embryonic development. Here we report the cloning of a C. elegans klp-12 cDNA, encoding an ortholog of chicken Chromokinesin and mouse KIF4. The KLP-12 protein contains 1609 amino acid and harbors two leucine zipper motifs. The in situ RNA hybridization in embryonic stages shows that the klp-12 gene is expressed during the entire embryonic development. The RNA interference assay reveals that, similar to the role of Chromokinesin, klp-12 functions in chromosome segregation. These results support the notion that during mitosis both types, the anterograde N-terminus kinesins such as KLP-12 and the retrograde C- terminus kinesins, such as KLP-3, KLP-15, KLP-16, and KLP-17, may coordinate chromosome assembly at the metaphase plate and chromosomal segregation towards the spindle poles in C. elegans.