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( Shanmugam Sureshkumar ),( Yan Jie Liu ),( Ning Bo Chen ),( In Ho Kim ) 한국축산학회(구 한국동물자원과학회) 2021 한국축산학회지 Vol.63 No.4
The objective of this study is to investigate the effect of dietary glucose oxidase (GOx) supplementation on the reproductive performance, litter performance, total tract digestibility, and blood profile of lactating sows fed corn- wheat-based diet. A total of twenty multiparous sows (Landrace × Yorkshire) were allocated into one of four treatments with five replicates per treatment. The dietary treatments were as follows: CON (Basal diet), GO1 (basal diet + 200 U GOx/kg), GO2 (basal diet + 300 U GOx/kg), GO3 (basal diet + 400 U Gox/kg). Dietary GOx supplementation did not affect lactating sow’s reproduction performance as well as body weight, backfat thickness, and body condition score during pre and post farrowing, and at weaning (p > 0.05). However, after farrowing to weaning period lactating sow’s fed GOx supplement has linearly (p = 0.0196) decreased the bodyweight loss. While, there were no effects (p > 0.05) observed on sows backfat thickness loss, average daily feed intake, and estrus interval among treatment groups. Dietary supplementation of GOx has linearly improved the body weight gain (p = 0.049) and average daily gain (p = 0.040) of suckling piglets. The total tract digestibility of dry matter and nitrogen was linearly increased with the graded level of GOx supplement. Also, a linear effect was observed on the glucose and superoxide dismutase of blood profile with the dietary inclusion of GOx. In summary, our finding indicates that the dietary inclusion of GOx supplement with corn- wheat-based diet had a beneficial effect on the nutrient digestibility and blood profile of lactating sows and improved the growth performance of suckling piglets.
Madesh Muniyappan,Sureshkumar Shanmugam,In Ho Kim 한국축산학회 2024 한국축산학회지 Vol.66 No.1
This study investigated the impact of clay mineral ‘illite’ (IL) on the growth performance, apparent total tract digestibility, and meat-carcass grade quality in growing-finishing pigs. One hundred fifty pigs were divided into two groups with fifteen pens/group and five pigs/pen, a control group that was fed with a corn-soybean meal-based diet and IL treated group were fed a meal-based diet supplemented with 0.5% IL. Compared to the control, IL supplementation increased average daily feed intake (ADFI) and reduce gain to feed ratio (G:F) in the pigs during days 43 to 70 and 99 to 126 and increased dry matter during days 42 and 126, and backfat thickness on day 98 (p < 0.05). The drip loss was reduced on day 7, meat firmness tended to increase with dietary IL supplementation. In summary, dietary 0.5% IL supplementation improved ADFI, nutrient utilization of dry matter, and firmness and reduced G:F and drip loss of growing-finishing pigs.
Lee, Gunsup,Choi, Hoonsung,Sureshkumar, Shanmugam,Jung, Sun Keun,Kim, Jeom Sun,Oh, Keon Bong,Kim, Kyung-Woon,Yang, Hyeon,Kim, Dong-Hoon,Byun, Sung June Elsevier 2019 Research in veterinary science Vol.123 No.-
<P><B>Abstract</B></P> <P>Infectious bronchitis (IB) generated by the infectious bronchitis virus (IBV) causes economic difficulties for livestock farmers. The 3D8 single chain variable fragment (scFv) protein is a recombinant antibody with nuclease activity that shows antiviral effects against various DNA and RNA viruses in mice and chickens. In this experiment, 3D8 scFv G<SUB>2</SUB> transgenic chickens produced by crossing 3D8 scFv G<SUB>1</SUB> transgenic rooster and wild type hens were screened by genomic PCR and immunohistochemistry analysis. 3D8 scFv transgenic chickens, wild type sibling chickens, and SPF chickens were directly infected with IBV (5 chickens per group) and indirectly infected by airborne propagation (15 chickens per group). The relative IBV shedding titers were measured by quantitative real-time PCR using oropharyngeal and cloacal swabs on days 3 and 5 after intraocular infection. The viral load was significantly decreased in the 3D8 scFv transgenic chickens from the contact transmission group. Additionally, blood was collected from each group on day 17 post-infection. The ELISA results showed a marked reduction of the antibody titer against IBV in the 3D8 scFv transgenic chickens from the contact transmission group. These results suggest that the 3D8 scFv protein potentially inhibits infectious bronchitis virus transmission in chickens.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Produced G2 3D8 single chain variable fragment (scFv) transgenic chickens. </LI> <LI> 3D8 scFv transgenic chickens showed reduced infectious bronchitis viral shedding level in the contact transmission group. </LI> <LI> 3D8 scFv transgenic chickens were 40% lower than the response in the control groups in IBV serum antibody titer. </LI> </UL> </P>
Enhancement of Semantic Interoperability in Healthcare Systems Using IFCIoT Architecture
Sony P,Siva Shanmugam G,Sureshkumar Nagarajan 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.4
Fast decision support systems and accurate diagnosis have become significant in the rapidly growing healthcare sector. As the number of disparate medical IoT devices connected to the human body rises, fast and interrelated healthcare data retrieval gets harder and harder. One of the most important requirements for the Healthcare Internet of Things (HIoT) is semantic interoperability. The state-of-the-art HIoT systems have problems with bandwidth and latency. An extension of cloud computing called fog computing not only solves the latency problem but also provides other benefits including resource mobility and on-demand scalability. The recommended approach helps to lower latency and network bandwidth consumption in a system that provides semantic interoperability in healthcare organizations. To evaluate the system's language processing performance, we simulated it in three different contexts. 1. Polysemy resolution system 2. System for hyponymy -hypernymy resolution with polysemy 3. System for resolving polysemy, hypernymy, hyponymy, meronymy, and holonymy. In comparison to the other two systems, the third system has lower latency and network usage. The proposed framework can reduce the computation overhead of heterogeneous healthcare data. The simulation results show that fog computing can reduce delay, network usage, and energy consumption.
( Jae Hong Park ),( Shanmugam Sureshkumar ),( In Ho Kim ) 한국축산학회(구 한국동물자원과학회) 2021 한국축산학회지 Vol.63 No.3
The aim of this was evaluate the efficacy of lysozyme on growth performance, nutrient digestibility, excreta microflora population, and blood profiles of weanling pigs under Escherichia coli (E. coli) challenge. A total of 30 piglets weaned at 25 days, 7.46 kg body weight, were assigned to three dietary treatments, composed of five replications, two piglets per replication, for 7 days. The dietary treatment groups were negative control (NC; without antibiotics and lysozyme), positive control (PC; NC + antibiotics), lysozyme (NC + 0.1% lysozyme). All piglets were challenged orally with 6 ml suspension, containing E. coli K88 (2 × 10<sup>9</sup> CFU/mL). Dietary supplementation with lysozyme and PC resulted in no significant differences in average daily gain and gain to feed efficiency. Weanling pigs fed with E. coli challenge with lysozyme and PC treatments had significantly enhanced nutrient retentions of dry matter and energy (p < 0.05); however, there was a tendency to increase nitrogen digestibility. Furthermore, dietary inclusion of lysozyme and antibiotics treatment groups had a beneficial effect on excreta, ileal, and cecal of the fecal microbial population as decreased E. coli (p < 0.05) counts, without effects on lactobacillus counts. A significant effect were observed on a white blood cells, epinephrine and cortisol concentrations were reduced in piglets fed diets containing E. coli challenge with lysozyme and antibiotics supplementation comparison with the NC group. Therefore, the present data indicate that lysozyme in diet could ameliorate the experimental stress response induced by E. coli in piglets by decreasing intestinal E. coli, white blood cells and stress hormones and improving nutrient digestibility.
Yang, Hyeon,Lee, Bo Ram,Lee, Hwi-Cheul,Jung, Sun Keun,Kim, Ji-Youn,No, Jingu,Shanmugam, Sureshkumar,Jo, Yong Jin,Lee, Haesun,Hwang, Seongsoo,Byun, Sung June Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.8
Objective: Transgenic hens hold a great promise to produce various valuable proteins. Through virus transduction into stage X embryo, the transgene expression under the control of constructed chicken ovalbumin promoters has been successfully achieved. However, a validation system that can evaluate differently developed ovalbumin promoters in in vitro, remains to be developed. Methods: In the present study, chicken oviduct epithelial cells (cOECs) were isolated from oviduct tissue and shortly cultured with keratinocyte complete medium supplemented with chicken serum. The isolated cells were characterized with immunofluorescence, western blot, and flow cytometry using oviduct-specific marker. Chicken mutated ovalbumin promoter (Mut-4.4-kb-pOV) was validated in these cells using luciferase reporter analysis. Results: The isolated cOECs revealed that the oviduct-specific marker, ovalbumin protein, was clearly detected by immunofluorescence, western blot, and flow cytometry analysis revealed that approximately 79.40% of the cells contained this protein. Also, luciferase reporter analysis showed that the constructed Mut-4.4-kb-pOV exhibited 7.1-fold (p<0.001) higher activity in the cOECs. Conclusion: Collectively, these results demonstrate the efficient isolation and characterization of cOECs and validate the activity of the constructed ovalbumin promoter in the cultured cOECs. The in vitro validation of the recombinant promoter activity in cOECs can facilitate the production of efficient transgenic chickens for potential use as bioreactors.