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피부손상 치유시 진피층내 Fibronectin, Type I 및 Type III Collagen의 발현에 대한 면역전자현미경적 연구
엄기일,조임철,김잉곤,정호심,이선우,류재만 韓國非破壞檢査學會 1998 Archives of Plastic Surgery Vol.25 No.7
This study was focused on the localizations or expressions of fibronectin and interactions of fibronectin with type I and type Ⅲ collagen in the new forming tissues during wound healing in rat skin. Adult male rats(SPrague-Dawley strain), 150∼200 gm weight, were used for experimental animals. And the experimental animals were divided into 3 or 4 groups by passed days after incision of back skin. The specimens were obtained and immunohistologically stained for electron microscopy from each of groups and the specimens were observed with the electron microscope (Hitach-600 Model). The results obtained were as follows. 1. Strong positive reactions for type I collagen are seen in the new forming tissue at day 3 after incision of skin, and moderate reactions at day 5 and day 7 after incision of skin. 2. The weak reactions for type Ⅲ collagen are seen at day 3 and day 5 after incision in the new forming tissues of skin. And the type Ⅲ collagen reaction at day 7 are more increased than that of other groups. 3. Fibronectin expressions are seen moderately at day 5 and day 3 groups, and strong at day 5 group, but at day 7 group reactions are rapidly decreased. It is suggested that type I and Ⅲ collagens are gradually increased from the beginning of wound healing to the time of the new fibrous tissues formation. The great activities of the fibronection are seen within processes of the new tissue formation for wound healing events.
Uhm, Jae-Sun,Jung, Hae-Ok,Kim, Chan-Joon,Kim, Tae-Hoon,Youn, Ho-Joong,Baek, Sang Hong,Chung, Wook-Sung,Seung, Ki Bae The Korean Academy of Medical Sciences 2012 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.27 No.11
<P>This study was performed to compare clinical and imaging parameters and prognosis of unprovoked pulmonary embolism (PE), provoked PE with reversible risk factors (provoked-rRF), and provoked PE with irreversible risk factors (provoked-iRF) in Koreans. Three hundred consecutive patients (mean age, 63.6 ± 15.0 yr; 42.8% male) diagnosed with acute PE were included. The patients were classified into 3 groups; unprovoked PE, provoked-rRF, and provoked-iRF; 43.7%, 14.7%, and 41.7%, respectively. We followed up the patients for 25.4 ± 33.7 months. Composite endpoint was all-cause mortality and recurrent PE. The provoked-iRF group had significantly higher all-cause mortality, mortality from PE and recurrent PE than the unprovoked and provoked-rRF groups (<I>P</I> < 0.001, <I>P</I> < 0.001, and <I>P</I> = 0.034, respectively). Prognostic factors of composite endpoint in the unprovoked group were high creatinine (> 1.2 mg/dL; <I>P</I> < 0.001; hazard ratio [HR], 4.735; 95% confidence interval [CI], 1.845-12.152), C-reactive protein (CRP; > 5 mg/L; <I>P</I> = 0.002; HR, 5.308; 95% CI, 1.824-15.447) and computed tomography (CT) obstruction index (<I>P</I> = 0.034; HR, 1.090; 95% CI, 1.006-1.181). In conclusion, provoked-iRF has a poorer prognosis than unprovoked PE and provoked-rRF. Renal insufficiency, high CRP, and CT obstruction index are poor prognostic factors in unprovoked PE.</P>
Uhm, Jae-Sun,Youn, Ho-Joong,Chung, Woo-Baek,Choi, Yun-Seok,Park, Chul-Soo,Oh, Yong-Seog,Chung, Wook-Sung,Park, Kyung-Il,Kim, Tae-Suk The Korean Association of Internal Medicine 2012 The Korean Journal of Internal Medicine Vol.27 No.1
<P><B>Background/Aims</B></P><P>This study elucidated the prognostic factors for neurocardiogenic syncope in males in their late teens and early twenties.</P><P><B>Methods</B></P><P>Tilt-table testing (TTT) was performed on 665 males (age range, 17 to 27 years) following the Italian protocol. The subjects were tilted head-up at a 70° angle on a table for 30 minutes during the passive phase. If the passive phase was negative, the subjects were given sublingual nitroglycerin and tilted to the same angle for 20 minutes during the drug-provocation phase. The subjects with positive results were followed without medication. We analyzed factors related to the recurrence rate of syncope.</P><P><B>Results</B></P><P>Of 305 subjects (45.8%) with positive results, 223 (age range, 18 to 26 years) were followed for 12 months. The frequency of previous syncopal episodes ≥ 4 (<I>p</I> = 0.001) and a positive result during the passive phase (<I>p</I> = 0.022) were significantly related to a high recurrence rate. A positive result during the early passive phase (≤ 12 minutes) was significantly related to a higher recurrence rate than was that during the late passive phase (> 12 minutes; <I>p</I> = 0.011).</P><P><B>Conclusions</B></P><P>A positive result during the early passive phase of TTT and frequent previous syncopal episodes were prognostic factors for neurocardiogenic syncope in men in their late teens and early twenties.</P>
( Jae Sun Uhm ),( Ho Joong Youn ),( Woo Baek Chung ),( Yun Seok Choi ),( Chul Soo Park ),( Yong Seog Oh ),( Wook Sung Chung ),( Kyung Il Park ),( Tae Suk Kim ) 대한내과학회 2012 The Korean Journal of Internal Medicine Vol.27 No.1
Background/Aims: This study elucidated the prognostic factors for neurocardiogenic syncope in males in their late teens and early twenties. Methods: Tilt-table testing (TTT) was performed on 665 males (age range, 17 to 27 years) following the Italian protocol. The subjects were tilted head-up at a 70° angle on a table for 30 minutes during the passive phase. If the passive phase was negative, the subjects were given sublingual nitroglycerin and tilted to the same angle for 20 minutes during the drugprovocation phase. The subjects with positive results were followed without medication. We analyzed factors related to the recurrence rate of syncope. Results: Of 305 subjects (45.8%) with positive results, 223 (age range, 18 to 26 years) were followed for 12 months. The frequency of previous syncopal episodes ≥ 4 (p = 0.001) and a positive result during the passive phase (p = 0.022) were significantly related to a high recurrence rate. A positive result during the early passive phase (≤ 12 minutes) was significantly related to a higher recurrence rate than was that during the late passive phase (> 12 minutes; p = 0.011). Conclusions: A positive result during the early passive phase of TTT and frequent previous syncopal episodes were prognostic factors for neurocardiogenic syncope in men in their late teens and early twenties.
OB-10 : Maternal vascular underperfusion: clinical implications in a preterm birth cohort
( Joon Ho Lee ),( Jeong Eun Kwon ),( Jee Yoon Park ),( Yeo Rang Kim ),( Yoo Kyung Uhm ),( Tae Kye Ahn ),( Joo Yeon Jeong ),( Sun Min Kim,),( Chan Wook Park ),( Joong Shin Park ),( Jong Kwan Jun ),( Bo 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: To determine the frequency and clinical implications of placental pathologic findings consistent with maternal vascular underperfusion (MVU) in preterm gestations. 방법: Placental pathologic findings consistent with MVU and obstetric and neonatal outcomes were reviewed in a consecutive preterm birth cohort with singleton gestation from Seoul National University Hospital (N=1,206). MVU was divided into two categories: vascular change and villous change (vascular MVU and villous MVU). 결과: The frequency of vascular MVU and villous MVU was 6.1% (74/1206) and 16.3% (196/1206), respectively. Cases with vascular MVU and villous MVU had lower median gestational age at delivery and birthweight than those without these lesions, respectively (p<0.01, for each). Vascular MVU and villous MVU were associated with iatrogenic preterm birth due to maternal and/or fetal indications, hypertensive disorders in pregnancy, cesarean delivery and small-for-gestational-age (SGA) newborns. Cases with vascular MVU had higher rates of neonatal respiratory distress syndrome (RDS), bronchopulmonary dysplasia (BPD) and necrotizing enterocolitis (NEC) than those without this lesion, which remained significant after adjusting for gestational age at delivery. Cases with villous MVU had a higher rate of NEC than those without this lesion after adjusting for gestational age at delivery. 결론: Placental pathologic findings consistent with maternal vascular underperfusion are associated with hypertensive disorders in pregnancy, SGA neonates and various adverse neonatal outcomes. These findings suggest that meticulous placental histopathologic examinations may be beneficial to elucidate mechanisms of adverse pregnancy outcomes.
Generation of Embryonic Stem Cell-derived Transgenic Mice by using Tetraploid Complementation
Park Sun-Mi,Song Sang-Jin,Choi Ho-Jun,Uhm Sang-Jun,Cho Ssang-Goo,Lee Hoon-Taek 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1
The standard protocol for the production of transgenic mouse from ES-injected embryo has to process via chimera producing and several times breeding steps, In contrast, tetraploid-ES cell complementation method allows the immediate generation of targeted murine mutants from genetically modified ES cell clones. The advantage of this advanced technique is a simple and efficient without chimeric intermediates. Recently, this method has been significantly improved through the discovery that ES cells derived from hybrid strains support the development of viable ES mice more efficiently than inbred ES cells do. Therefore, the objective of this study was to generate transgenic mice overexpressing human resistin gene by using tetrapioid-ES cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR and cloned into pCR 2.1 TOPO T-vector and constructed in pCMV-Tag4C vector. Human resistin mammalian expression plasmid was transfected into D3-GL ES cells by lipofectamine 2000, and then after 8~10 days of transfection, the human resistin-expressing cells were selected with G418. In order to produce tetraploid embryos, blastomeres of diploid embryos at the two-cell stage were fused with two times of electric pulse using 60 V 30 sec. (fusion rate : 93.5%) and cultured upto the blastocyst stage (development rate : 94.6%). The 15~20 previously G418-selected ES cells were injected into tetraploid blastocysts, and then transferred into the uterus of E2.5d pseudopregnant recipient mice. To investigate the gestation progress, two El9.5d fetus were recovered by Casarean section and one fetus was confirmed to contain human resistin gene by genomic DNA-PCR. Therefore, this finding demonstrates that tetraploid-ES mouse technology can be considered as a useful tool to produce transgenic mouse for the rapid analysis of gene function in vivo.
Song, Yoon-Ho,Cho, Young-Rae,Hwang, Chi-Sun,Kim, Bong-Chul,Ahn, Seong-Deok,Chung, Choong-Heui,Kim, Do-Hyung,Uhm, Hyun-Seok,Lee, Jin-Ho,Cho, Kyoung-Ik The Korean Infomation Display Society 2001 Journal of information display Vol.2 No.3
Amorphous silicon thin-film transistors (a-Si TFTs) were incorporated into Mo-tip-based triode-type field emitters and diode-type ones of carbon nanotubes for an active-matrix cathode (AMC) plate of field emission displays. Also, we developed a novel surface-treatment process for the Mo-tip fabrication, which gleatly enhanced in the stability of field emission. The field emission currents of AMC plates on glass substrate were well controlled by the gate bias of a-Si TFTs. Active-matrix field emission displays (AMFEDs) with these AMC plates were demonstrated in a vacuum chamber, showing low-voltage matrix addressing, good stability and reliability of field emission, and highly uniform light emissions from the anode plate with phosphors. The optimum design of AMFEDs including a-Si TFTs and a new light shield/focusing grid is discussed.
Yoon, Jin Sun,Hwang, Deok Won,Kim, Eun Shil,Kim, Jung Soon,Kim, Sujong,Chung, Hwa Jin,Lee, Sang Kook,Yi, Jun Ho,Uhm, Jieun,Won, Young Woong,Park, Byeong Bae,Choi, Jung Hye,Lee, Young Yiul Springer-Verlag 2016 Investigational new drugs Vol.34 No.1
<P>Arsenic compounds have been used in traditional medicine for several centuries. KML001 (sodium metaarsenite; NaAsO2) is an orally bio-available arsenic compound with potential anti-cancer activity. However, the effect of KML001 has not been studied in lymphoid neoplasms. The aim of this study is to evaluate the anti-proliferative effect of KML001 in non-Hodgkin's lymphoma and to compare its efficacy with As2O3. KML001 inhibited cellular proliferation in all tested lymphoma cell lines as well as JurkatR cells (adriamycin-resistant Jurkat cells) in a dose-dependent manner, while As2O3 was not effective. Cell cycle regulatory protein studies have suggested that KML001 induces G1 arrest via p27-induced inhibition of the kinase activities of CDK2, 4, and 6. Treatment of KML001 induced apoptosis in Jurkat and JurkatR cells. The apoptotic process was associated with down-regulation of Bcl-2 (antiapoptotic molecule), up-regulation of Bax (proapoptotic molecule), and inhibition of caspase-3, -8, and -9. In addition, cell signaling including the STAT, PI3K/Akt, MAPK, and NF-kappa B signal pathways were inhibited in KML001-treated Jurkat and JurkatR cells. Furthermore, targeting the telomere by KML001 was observed in the Jurkat and JurkatR cells. The In vivo anti-tumoral activity of KML001 was confirmed in a xenograft murine model. Interestingly, partial responses were seen in two lymphoma patients treated with 10 mg/day (follicular lymphoma for 16 weeks and mantle cell lymphoma for 24 weeks) without severe toxicities. These findings suggest that KML001 may be a candidate agent for the treatment of de novo, refractory, and relapsed non-Hodgkin's lymphoma patients.</P>