http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Soyoung Kang,Deok-Ho Kwon,Kyung-Seok Kim,Si Hyeock Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
To search for hyper-variable genetic markers that can distinguish regional populations of head lice, we screened the inter simple sequence repeats (ISSR) based on the genome database of body louse, which is closely related conspecific species. An ISSR mining software, SciRoKo 3.4, was employed to excavate ISSR markers from the genome database under the MISA mode (≥ 60 bp repeats). Entire body louse genome (ca.100 Mb) was loaded to SciRoKo for ISSRs mining. A total of 5,336 ISSRs were obtained, and primers specific to individual ISSRs were designed by the Primer 3 and DesignPrimer 1.0 softwares. In order to prove the compatibility of body louse ISSRs to head lice, 31 PCR primers were randomly chosen out of a total of 613 pairs, and their appropriateness was tested by comparing the amplified PCR band patterns between body and head lice. Eleven primer pairs that resulted in poor or little amplification were excluded, and 20 primer pairs were further tested for three head louse populations (California, Panama and Chung-ju, Korea). Finally, nine primer pairs ensuring robust amplification of highly variable band patterns were selected to use for population genetic study of head lice.
Comprehension of Ambiguous English Participle Constructions by Korean Learners of English
Soyoung Kang 한국영어학학회 2014 영어학연구 Vol.20 No.1
The current study reports the results of two off-line written experiments that examined baseline preference in the interpretation of ambiguous English participle constructions by Korean learners of English. Participants read a list of sentences containing ambiguous English participle phrases and answered comprehension questions designed to show their attached comprehension preference. The results showed that Korean learners of English displayed no clear bias toward either interpretation with only a weak trend toward the low attached reading.
Soyoung Kang,Hye Jung Lee,김영호,Jung Hun Oh,Bum Jun Kim,Kook Jin Lim,Seunghwan Lee,황승용,이시혁,권덕호 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.3
Proteomic differences between Bemisia tabaci biotypes (B and Q) were investigated by two-dimensional gel electrophoresis in conjunction with mass spectroscopic analysis. Among several protein spots specific to biotype B, carboxylesterase 2 (Coe2) was significantly more expressed in biotype B. Phylogenetic analysis demonstrated the close relationship of Coe2 with Myzus persicae esterase E4. Comparison of full-length cDNA sequences of Coe2 revealed no amino acid differences in functionally important conserved regions between biotypes B and Q. The transcription level of the Coe2 gene (coe2) was 5.8-fold higher in biotype B than in biotype Q, but the coe2 copy number was not different between biotypes, suggesting that the overexpression of Coe2 was due to transcriptional up-regulation. Native isoelectric focusing followed by mass spectrophotometric analysis confirmed that the overexpressed pI 5.7 esterase in biotype B was Coe2. In-gel inhibition of Coe2 by three insecticides indicated the interaction of Coe2 with chlorpyrifos-methyl oxon and permethrin,but not with imidacloprid. These findings suggest that overexpression of Coe2 in biotype B can confer chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration and hydrolysis,as seen in M. persicae E4. Finally, utility of Coe2 as a potential biotype-specific protein marker is discussed.
Development of protein and DNA markers for the identification of Bemisia tabaci biotypes B and Q
Soyoung Kang,Bum Joon Kim,Hye Jung Lee,Si Hyeock Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. In order to search for protein markers that can be employed for rapid and accurate diagnosis of biotypes, two-dimensional PAGE (2DE) in conjunction with mass spectroscopic analysis were conducted. Eleven biotype-specific spots were repeatedly identified during three repetitions of 2DE and analyzed by Q-TOF. One of the B type-specific protein spots was identified as carboxylesterase 2 (Coe2). The transcript level of coe2 was determined to be 6 times higher in B type than in Q type by quantitative real-time PCR. In addition, comparison of genomic DNA sequence of coe2 between B and Q types identified a biotype-specific intron, from which specific primer sets were designed. One-step PCR using these biotype-specific primers successfully distinguished the two biotypes in a high accuracy. Availability of the biotype-specific protein and DNA markers will greatly improve the detection of B. tabaci biotype in the field.
Individual differences in autistic traits and variability in production patterns
Kang, Soyoung,Kong, Eun Jong,Seo, Misun 한국음성학회 2015 말소리와 음성과학 Vol.7 No.1
The current study explores whether speaker variability in the fronted articulations of Seoul Korean affricates can be explained by cognitive differences measured by individual autistic traits. The goal was to explore Yu’s (2010; 2013) proposal that individual differences in cognitive style can be an important factor in speakers’ use of sound variants. The spectral peak frequencies (SPF) of affricates relative to those of fricatives, reported in Kong et al. (2014), were used to acoustically represent the relative degree of anterior place of constriction. When these individual SPFs were related to the scores of Autistic-Spectrum Quotients (Baron-Cohen et al., 2001), a correlation was found for the male speakers, but not for the female speakers, such that speakers of more anterior affricate productions scored low in AQs. Discussion is made with respect to how these findings are in line with Yu’s proposal.
Preschoolers’ Online Video Consumption Patterns and Self-Regulation
Soyoung Kang,Kayoun Chung,JinSil Lee The Pacific Early Childhood Education Research Ass 2023 Asia-Pacific journal of research in early childhoo Vol.17 No.1
The study was to understand how online video consumption patterns affect preschoolers’ self-regulation. A questionnaire was developed based on other scales in previous studies. The survey was conducted from June 3 to June 24, 2019, with 263 mothers of 3, 4, 5-year-old children enrolled in preschool and day care centers in South Korea. The analysis of the collected data was performed on SPSS 21.0, calculating frequencies and percentages and applying the t-test and one-way ANOVA. A considerable proportion of the respondents (41.4%) reported they permitted their child to use the online video platform before 24 months of age. Most children watched online video following the rules that their parents established (75.7%). Significant differences were found in children’s self-regulation depending on the presence or absence of the rules at home, the amount of screen time limit, when to watch during the day or during weekdays. This study provides empirical evidence to set preliminary guidelines for the optimal viewing conditions for preschoolers to avoid online video addiction.
The putative role of carboxylesterase 2 in insecticide tolerance in Bemisia tabaci
Soyoung Kang,Deok-Ho Kwon,Jung Hun Oh,Si Hyeock Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The hemipteran whitefly Bemisia tabaci (Gennadius) is one of the most destructive pests damaging more than 600 agricultural crop species worldwide. The B and Q biotypes are most widely spread in Korea but they are not distinguishable based on morphological characters. Carboxylesterase 2 (Coe2) was determined to be 5.9 times more expressed in B biotype compared to Q biotype. Comparison of deduced amino acid sequences of Coe2 (595 a.a.) showed a total of 3.85% polymorphisms between B and Q types but no differences in major active sites. Quantitative real-time PCR revealed that both B and Q biotypes possess a single copy of coe2, suggesting that the overexpression of Coe2 in B biotype is likely due to overtranscription. To determine the putative role of Coe2 in insecticide tolerance, esterases were separated by native isoelectric focusing (IEF) and inhibited by various insecticides. The putative Coe2 band was apparently inhibited by pyrethroid and organophosphate insecticides, but not by imidacloprid. These findings suggest that overexpression of Coe2 confers chemical defense against pyrethroid and organophosphate insecticides, perhaps by sequestration.
Soyoung Kang,김영호,Hye Jung Lee,Bum Jun Kim,Kook Jin Lim,이시혁 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.3
Sequence and length-polymorphic intron variations in the caboxylesterase 2 gene (coe2) were determined to be specific to the biotypes B and Q of Bemisia tabaci. By employing the biotype-specific coe2 intron variation as a nuclear marker, a one-step diagnostic protocol for the identification of B and Q biotypes was developed and its performance was validated for field collected B. tabaci specimens. The diagnostic results based on the coe2 intron marker were identical to those obtained from the mtCOI marker in all 256 specimens examined except for four individuals that appeared to be putative heterozygotes between B and Q biotypes. These results demonstrate a high level of accuracy of the coe2 intron marker-based protocol in distinguishing biotypes B and Q. Moreover, because the process requires only PCR amplification and gel electrophoresis,analysis of multiple samples can be done more efficiently. Based on the observation that all putative heterozygotes have the maternal background of Q biotype, they may have been created by inter-biotype cross between B type male and Q type female. If combined with the mtCOI marker, the nuclear coe2 marker would provide a better resolution than maternally inherited markers alone and facilitate the demographic study of B. tabaci biotype complex.