사무직 근로자들의 직무 스트레스와 피로

차경태,김일희,고상백,현숙정,박준호,박종구,차봉석,장세진 大韓産業醫學會 2008 대한직업환경의학회지 Vol.20 No.3

목적: 이 연구는 전국 규모의 조사 연구에서 수집된 자료 중 사무직 근로자들을 대상으로,사회인구학적 특성,직업 특성,건강행태,직무 스트레스와 피로수준 간의 관련성을 분석하는 데 있다. 방법:‘한국인 직무스트레스 측정도구의 표준화 전국 조사연구National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002∼2004)’를 위해 2002년 5월 1일부터 2003년 5윌 30일 까지 수행되었으며,전국의 사업장에 근무하는 근로자 30,146명(남자: 84%,여자: 16%) 중 사무직 근로자 4,457명(남자: 68.9%,여자 31.1%)을 연구 대상으로 하였다. 자료수집방법은 사업장을 방문하여 보건 및 안전관리자 등에게 연구의 취지를 알린 후 설문조사에 대한 협조를 구하고 응답자 직접 기입법을 이용 하였으며,사회인구학적 특성,직업적 특성,건강관련 요인,KOSS-SF를 이용한 7개의 직무 스트레스 요인,그리고 MFS를 이용하여 피로 수준에 영향을 주는 요인에 대해 분석하였다. 결과: 일반적 특성,작업관련 특성,건강행태관련 특성 및 직무 스트레스 요인이 근로자들의 피로와 강한 관련성이 있음이 입증되었으며,특히 직무 스트레스의 하부 요인 중에서도 피로와 관련성을 보이는 요인이 남녀별로 다소 상이 하게 관련된다는 것을 발견할 수 있었다. 남자의 경우는 직무 스트레스의 하부 영역 중 직무 요구도,직무 불안정성,보상부적절 등이 영향력이 높은 주요 직무 스트레스 요인이었던 반면,여자는 직무 요구도,보상부적절,직장문화가 주요한 직무 스트레스 요인이었다. 결론: 직장인 피로가 갖는 보건학적 의학적 경제학적 중요성을 감안할 때,조직의 생산성 향상과 근로자 개개인의 삶의 질 향상 및 건강증진을 위해선 직무 스트레스 관리 프로그램이 절실히 요청된다고 볼 수 있다. Background & Objectives: A growing body of research has documented that occupational stress is closely associated with increased risk of fatigue. This study was conducted in an effort to assess the relationship of occupational stress to self-perceived fatigue among Korean white collar employees. Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002-2004). Among them, a total of 4,502 white collar employees were recruited. A structured questionnaire was employed to evaluate the participants' sociodemographics, job-related factors, health-related behaviors, occupational stress, and self-perceived fatigue. Occupational stress and self-perceived fatigue were assessed using the Korean Occupational Stress ScaleShort form (KOSS-SF) and the Multidimensional Fatigue Scale (MFS), respectively. Results: In logistic regression analyses, occupational stress was associated with an increased risk of fatigue, and some domains of occupational stress had different effects on fatigue by gender (job demand, job insecurity, and lack of reward for men, job demand, lack of reward, and discomfort in occupational climate for women), which indicates that occupational stress may perform a slightly different role in increasing the risk of fatigue by gender. Conclusion: The results of this study suggest that occupational stress is a determinant predictor of self-perceived fatigue. Thus, a stress management program for the reduction of occupational stress, and the promotion of white collar worker's health and quality of life is strongly recommended. Some limitations of this study and considerations for future studies were also discussed.

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.

Behavioral Response of Termites to Tunnel Intersection

Sook Jung Ku,Sheon-Young Kang,Nan-Yao Su,Sang-Hee Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Subterranean termites construct complicated tunnel network for foraging below the ground. Thus, they often encounter a number of tunnel intersections during their moving from place to place in the network. In order to understand how termites respond to the intersections, we artificially excavated two tunnels intersected with 90° degree in soil-filled arenas. The two tunnels had the width of W1 and W2 (=2, 3, and 4mm), respectively. We systematically observed the response behavior of advancing termites to the intersection with the combination of W1 and W2, (W1, W2). For (W1, W2)=(2, 2) and (3, 3), the advancing termites passed the intersection without directional changes because it was difficult for termites to bend their body to change their moving direction due to the small-sized width. For (W1, W2)=(4, 4), the termites statistically-equally chose the three directions, left, right, and straight, which was due to the fact that the intersection provided enough space for termites to bend. For (W1, W2)=(2, 3), (2, 4), and (3, 4), termites, advancing in narrower tunnels, tended considerably to turn right or left, while termites, advancing in wider tunnels, were favorably inclined to go straight. These results can be understood by considering the relationship between termite body length and tunnel width as explained for the cases of W1=W2. In addition, we briefly discussed our findings in relation to termite foraging efficiency.

Fucoidan의 멜라닌 합성과 tyrosinase 활성도 억제 효과

정숙희(Sook Hee Jung),구미정(Mi Jung Ku),문희정(Hee Jung Moon),유병철(Byeng Chul Yu),전만중(Man Joong Jeon),이용환(Yong Hwan Lee) 한국생명과학회 2009 생명과학회지 Vol.19 No.1

미역, 다시마 등 갈조류의 추출물로서 생리활성성분으로 각광 받고 있는 fucoidan이 멜라닌 합성에 미치는 영향을 알아봄으로써 미백제로서의 개발 가능성 여부를 확인하기 위하여 B16F10 melnoma 세포를 이용하여 멜라닌 합성과 tyrosinase 활성도에 대한 실험의 결과 B16F10 melnoma 세포의 멜라닌 생성과 tyrosinase 활성도는 fucoidan의 농도가 증가함에 따라 농도 의존적으로 억제되었다. B16F10 melnoma 세포에 α-MSH를 투여 후 멜라닌 생성과 tyrosinase의 활성도 역시 fucoidan의 농도가 증가할수록 멜라닌 생성과 tyrosinase활성도가 억제되는 경향을 보였다. 또한 DOPA 염색의 결과 fucoidan은 농도 의존적으로 tyrosinase 활성도를 억제하였다. 이상의 결과 해조 추출 다당류인 fucoidan은 B16F10 melanoma 세포의 멜라닌 합성과 tyrosinase 활성도 억제 작용을 나타내므로 미백 활성 물질로서의 가능성을 가지고 있는 것으로 생각된다. Melanogenesis is a physiological process that results in the synthesis of melanin pigments. Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. Among the possible melanin-reducing compounds, tyrosinase inhibitors are most promising for preventing and treating pigmentation disorder and are used as skin-whitening agents in the cosmetic industry. In the present study, the effects of fucoidan on melanogenesis and tyrosinase activity of B16F10 melanoma cells were investigated. Melanin synthesis and tyrosinase activity in B16F10 melanoma cells were decreased in a dose-dependent manner by fucoidan. Melanin production and tyrosinase activity in B16F10 melanoma cells stimulated by α-melanocyte stimulating hormone (α-MSH) were inhibited by fucoidan with a dose-dependent manner compared to control. Fucoidan inhibited tyrosinase activity of B16F10 melanoma cells with a dose-dependent manner as assessed by 3,4-dihydroxyphenylalanine (DOPA) staining. In conclusion, these findings indicate that fucoidan, which inhibit melanin synthesis and tyrosinase activity, is an effective skin-whitening agent.

Isolation and Identification of Fungi from a Meju Contaminated with Aflatoxins

( Yu Jung Jung ),( Soo Hyun Chung ),( Hyo Ku Lee ),( Hyang Sook Chun ),( Seung Beom Hong ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.12

A home-made meju sample contaminated naturally with aflatoxins was used for isolation of fungal strains. Overall, 230 fungal isolates were obtained on dichloran rosebengal chloramphenicol (DRBC) and dichloran 18% glycerol (DG18) agar plates. Morphological characteristics and molecular analysis of a partial β-tubulin gene and the internal transcribed spacer (ITS) of rDNA were used for the identification of the isolates. The fungal isolates were divided into 7 genera: Aspergillus, Eurotium, Penicillium, Eupenicillium, Mucor, Lichtheimia, and Curvularia. Three strains from 56 isolates of the A. oryzae/flavus group were found to be aflatoxigenic A. flavus, by the presence of the aflatoxin biosynthesis genes and confirmatory aflatoxin production by high-performance liquid chromatography (HPLC). The predominant isolate from DRBC plates was A. oryzae (42 strains, 36.2%), whereas that from DG18 was A. candidus (61 strains, 53.5%). Out of the 230 isolates, the most common species was A. candidus (34.3%) followed by A. oryzae (22.2%), Mucor circinelloides (13.0%), P. polonicum (10.0%), A. tubingensis (4.8%), and L. ramosa (3.5%). A. flavus and E. chevalieri presented occurrence levels of 2.2%, respectively. The remaining isolates of A. unguis, P. oxalicum, Eupenicillium cinnamopurpureum, A. acidus, E. rubrum, P. chrysogenum, M. racemosus, and C. inaequalis had lower occurrence levels of < 2.0%.

Effect of Dongchunghacho on Gene Expression in Aorta and Liver in ApoE -/- Mice Fed a High-fat/high-cholesterol Diet

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sook Jae Sea,Hong Ja Kim,Eunju Park,Gyeong Im Jeon,Hyun Jung Lee, 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

Protective Effects of Fucoidan against UVB-Induced Oxidative Stress in Human Skin Fibroblasts

Mi Jung Ku(구미정),Myeong Sook Lee(이명숙),Hee Jung Moon(문희정),Yong Hwan Lee(이용환) 한국생명과학회 2010 생명과학회지 Vol.20 No.1

자외선 B는 반응성 산소종(reactive oxygen species, ROS)의 발생을 유도하고 세포내 항산화 물질의 저장을 방해한다. 후코이단은 L-fucose와 황 에스터기를 포함하는 다당류로서 갈조류의 주요 성분으로 알려져 있다. 본 연구에서는 인간 피부 섬유아세포인 HS68세포에 자외선을 조사하여 산화 스트레스를 유도한 후 후코이단의 보호효과를 확인하고자 하였다. 후코이단의 전처치로 malondialdehyde (MDA)의 양이 용량-의존적으로 감소하였고, 세포내 glutathiopne도 후코이단 100 ㎍/ml을 투여하였을 때 후코이단을 투여하지 않고 자외선만 조사한 군에 비하여 21.5% 감소하였다. 후코이단을 10, 100 ㎍/ml의 용량으로 투여하였을 경우 자외선 B에 의한 ROS 생성이 자외선만 조사하였을 때 보다 각각 40.1%와 68.4%로 유의하게 감소하였다. 세포노화와 관련된 β-galactosidase의 양성 염색률은 후코이단을 10, 100 ㎍/ml 투여하였을 경우 자외선만 조사하였을 때보다 각각 23.1%, 16.4% 감소하였다. 이러한 결과로 미루어 후코이단은 자외선에 의한 산화 스트레스에 대하여 광보호 효과가 있는 것으로 생각된다. Ultraviolet-B (UVB) radiation induces the formation of reactive oxygen species (ROS) and depletes stores of cellular antioxidants. Fucoidan, polysaccharides containing L-fucose and sulfate ester groups, are constituents of brown algae. In this study, the protective effects of fucoidan on UVB-induced oxidative stress in cultured human skin fibroblast HS68 cells were assessed. Pretreatment with fucoidan significantly reduced malondialdehyde (MDA) content in a dose-dependent manner. With fucoidan pretreatment at a dose of 100 ㎍/ml, the level of intracellular glutathione was increased by 21.5%, compared to UVB irradiation alone. Fucoidan significantly reduced UVB-induced ROS generation by 40.1% and 68.4% at 10 and 100 ㎍/ml, respectively, compared to UVB irradiation alone. The positive staining rates of senescence-associated β-galactosidase were reduced by 23.1% and 16.4% with 10 and 100 ㎍/ml of fucoidan, compared to UVB irradiation alone. Fucoidan may exert a photoprotective effect against UVB radiation-induced oxidative stress.

Analysis of the Responses of Termites to Tunnel Irregularity

Sang-Hee Lee,Sook Jung Ku 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

Subterranean termites build extensive underground galleries consisting of elaborate tunnels and channels to forage food resources. Diverse soil conditions surrounding the tunnels, such as soil density, may cause irregularities in the size and shape of the tunnels, and termites are likely to encounter a number of tunnel irregularities while traveling. Considering the tunnel length, how termites respond to an irregularity is likely to affect their movement efficiency, and this in turn is directly correlated to their foraging efficiency. To understand the response of termites, we designed an artificial linear tunnel with rectangular irregularities in a 2-D arena. The tunnel widths, W, were 3 and 4 mm. The rectangular irregularities were 2 mm in width and of varying heights H (2, 1, 0, -1, and -2 mm). The positive and negative sign of H represents a convex and concave structure, respectively. We systematically observed the movement of termites, Coptotermes formosanus Shiraki, at the irregularity and quantified the time needed, τ, for a termite to pass the irregularity. The time τ was shorter for (W, H) = (3, 0) and (3, -1) than for (W, H) = (3, 1), (3, 2), and (3, -2). The time τ was longer for (W, H) = (4, -1), and (4, -2), than for (W, H) = (4, 0), (4, 1) and (4, 2). Four types of behaviors explained the response to the irregularity. The implications of these findings are briefly discussed in relation to termite foraging efficiency.

Novel Transcription Coactivator Complex Containing Activating Signal Cointegrator 1

Jung, Dong-Ju,Sung, Hee-Sook,Goo, Young-Wha,Lee, Hyun Mi,Park, Ok Ku,Jung, Sung-Yun,Lim, Janghoo,Kim, Han-Jong,Lee, Soo-Kyung,Kim, Tae Sung,Lee, Jae Woon,Lee, Young Chul 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

Human activating signal cointegrator 1 (hASC-1) was originally isolated as a transcriptional coactivator of nuclear receptors. Here we report that ASC-1 exists as a steady-state complex associated with three polypeptides, P200, P100, and P50, in HeLa neclei; stimulates transactivation by serum response factor (SRF), activating protein 1 (AP-1), and nuclear factor κB (NF-κB) through direct binding to SRF, c-Jun, p50, and p65; and relieves the previously described transrepression between nuclear receptors and either AP-1 or NF-κB. Interestingly, ectopic expression of Caenorhabditis elegans ASC-1 (ceASC-1), an ASC-1 homologue that binds P200 and P100, like hASC-1, while weakly interacting only with p65, in HeLa cells appears to replace endogenous hASC-1 from the hASC-1 complex and exerts potent dominant-negative effects on AP-1, NF-κB, and SRF transactivation. In addition, neutralization of endogenous P50 by single-cell microinjection of a P50 antibody inhibits AP-1 transactivation; the inhibition is relieved by coexpression of wild-type P50, but not of P50△KH, a mutant form that does not interact with P200. Overall, these results suggest that the endogenous hASC-1 complex appears to play an essential role in AP-1, SRF, and NF-κB transactivation and to mediate the transrepression between nuclear receptors and either AP-1 or NF-κB in vivo.

고환 및 고환주위 종양의 입상 및 병리조직학적 연구

서정일,박문향,박효숙,최구영 대한병리학회 1981 Journal of Pathology and Translational Medicine Vol.15 No.1