http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Monitoring of Bacterial Pathogens in Agricultural Products and Environments at Farms in Korea
( Shree Prasad Thapa ),( Seong Soo Kim ),( Sun Song Hong ),( Dong Sik Park ),( Chun Keun Lim ),( Jang Hyun Hur ) 한국응용생명화학회 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.3
A total of 142 samples comprising vegetables, soil, and water collected from different agricultural farms (five provinces) were analyzed for total aerobic bacteria (aerobic plate count [APC]), Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. The average of total APC in all the samples ranged from 4.72×105 to 8.62 ×108 CFU/g (mL). The prevalence of B. cereus, E. coli, L. monocytogenes, S. spp., and S. aureus for all samples was 17.60%, 2.11%, 1.4%, 0%, and 2.11% respectively, and their counts averaged to 4.87×104 CFU/g (mL), 4.34×103 CFU/g (mL), 2.15×102 CFU/g, 0 CFU/g, and 3.12×103 CFU/g respectively. Among the 3 different types of samples, 6 vegetables (10.34%), 24 soil (38.70%), and 3 water (13.64%) samples were found to be positive for bacterial pathogens. The result showed that the occurrence of bacterial pathogen in the samples analyzed was low. Further time to time monitoring and need to wash of raw agricultural products is recommended.
Study on Microbiological Condition of Fresh Produce at Different Stages of Packaging Sheds
Thapa, Shree Prasad,Kim, Seong-Soo,Park, Hong-Ryeol,Park, Dong-Sik,Lim, Chun-Keun,Hur, Jang-Hyun The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
A total of 223 produce samples and environmental swabs collected from various packaging sheds were examined by enumerating total aerobic plate count, total coliform, Escherichia coli and select bacterial pathogens; Bacillus cereus, E. coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. B. cereus isolates were further screened for carbohydrate utilization, antibiotics susceptibility, and presence of pathogenic gene. Total aerobic plate, total coliform, and E. coli counts of fresh produce ranged from 4.6 to 6.6, 1 to 3.0, and less than 1 to 2 log CFU/g, respectively. The prevalence of pathogens for all samples was 21.5%, and 0.89% for B. cereus and S. aureus, respectively. However, no pathogenic E. coli, L. monocytogenes, and Salmonella spp. were detected. Most of the B. cereus isolates identified were susceptible to penicillin, polymyxin B, ampicillin, oxacillin, and cefotaxime, whereas none of the isolates were positive for emetic toxin, an indication that cleansing procedure at the packaging sheds was not effective. Thorough sanitation and aseptic handling of fresh produce through out the packaging facilities is accentuated.
Thapa, Shree Prasad,Lee, Hye-Jin,Park, Duck-Hwan,Kim, Sam-Kyu,Cho, Jun-Mo,Cho, Sae-Youll,Hur, Jang-Hyun,Lim, Chun-Keun The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMV-Y inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
Monitoring of Bacterial Pathogens in Agricultural Products and Environments at Farms in Korea
Thapa, Shree Prasad,Kim, Seong-Soo,Hong, Sun-Song,Park, Dong-Sik,Lim, Chun-Keun,Hur, Jang-Hyun The Korean Society for Applied Biological Chemistr 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.3
A total of 142 samples comprising vegetables, soil, and water collected from different agricultural farms (five provinces) were analyzed for total aerobic bacteria (aerobic plate count [APC]), Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. The average of total APC in all the samples ranged from $4.72{\times}10^5$ to $8.62{\times}10^8\;CFU/g$ (mL). The prevalence of B. cereus, E. coli, L. monocytogenes, S. spp., and S. aureus for all samples was 17.60%, 2.11 %, 1.4%, 0%, and 2.11 % respectively, and their counts averaged to $4.87{\times}10^4\;CFU/g$ (mL), $4.34{\times}10^3\;CFU/g$ (mL), $2.15{\times}10^2\;CFU/g$, 0 CFU/g, and $3.12{\times}10^3\;CFU/g$ respectively. Among the 3 different types of samples, 6 vegetables (10.34%), 24 soil (38.70%), and 3 water (13.64%) samples were found to be positive for bacterial pathogens. The result showed that the occurrence of bacterial pathogen in the samples analyzed was low. Further time to time monitoring and need to wash of raw agricultural products is recommended.
Shree Prasad Thapa,이효진,박덕환,김삼규,조준모,조세열,허장현,임춘근 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4
The potential antiviral effects of the culture filtrates (CF) from Serratia marcescens strain Gsm01 against yellow strain of Cucumber mosaic virus (CMV-Y) were investigated. The culture filtrate of S. marcescens strain Gsm01 applied on Chenopodium amaranticolor showed high inhibitory activity, likewise no necrosis appeared when applied on the tobacco plants 2 days before CMVY inoculation. When plants were challenge inoculated with CMV-Y for eighteen days, the disease incidence in plants with culture filtrate of S. marcescens Gsm01 did not exceed 59%, whereas 100% of control plants were severely infected. The results of double antibody sandwich- enzyme linked immunosorbent assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RTPCR), dot blotting, and western blotting showed that culture filtrate treatment highly affected the accumulation of CMV-Y or its CP protein gene in the treated plant leaves. It was also observed that the culture filtrate had no RNase activity on genomic RNAs of CMV-Y, suggesting that culture filtrate may not contain ribosome inactivating proteins (RIPs) or proteins with RNase activity. These data shows that culture filtrate of S. marcescens strain Gsm01 seems to be a promising source of antiviral substance for the practical use.
Study on Microbiological Condition of Fresh Produce at Different Stages of Packaging Sheds
( Shree Prasad Thapa ),( Seong Soo Kim ),( Hong Ryeol Park ),( Dong Sik Park ),( Chun Keun Lim ),( Jang Hyun Hur ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
A total of 223 produce samples and environmental swabs collected from various packaging sheds were examined by enumerating total aerobic plate count, total coliform, Escherichia coli and select bacterial pathogens; Bacillus cereus, E. coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. B. cereus isolates were further screened for carbohydrate utilization, antibiotics susceptibility, and presence of pathogenic gene. Total aerobic plate, total coliform, and E. coli counts of fresh produce ranged from 4.6 to 6.6, 1 to 3.0, and less than 1 to 2 log CFU/g, respectively. The prevalence of pathogens for all samples was 21.5%, and 0.89% for B. cereus and S. aureus, respectively. However, no pathogenic E. coli, L. monocytogenes, and Salmonella spp. were detected. Most of the B. cereus isolates identified were susceptible to penicillin, polymyxin B, ampicillin, oxacillin, and cefotaxime, whereas none of the isolates were positive for emetic toxin, an indication that cleansing procedure at the packaging sheds was not effective. Thorough sanitation and aseptic handling of fresh produce through out the packaging facilities is accentuated.
Study on Microbiological Condition of Fresh Produce at Different Stages of Packaging Sheds
Shree Prasad Thapa,Seong-Soo Kim,Hong-Ryeol Park,Dong-Sik Park,임춘근,허장현 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
A total of 223 produce samples and environmental swabs collected from various packaging sheds were examined by enumerating total aerobic plate count, total coliform, Escherichia coli and select bacterial pathogens; Bacillus cereus, E. coli, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus. B. cereus isolates were further screened for carbohydrate utilization,antibiotics susceptibility, and presence of pathogenic gene. Total aerobic plate, total coliform, and E. coli counts of fresh produce ranged from 4.6 to 6.6, 1 to 3.0, and less than 1 to 2 log CFU/g, respectively. The prevalence of pathogens for all samples was 21.5%, and 0.89% for B. cereus and S. aureus, respectively. However, no pathogenic E. coli, L. monocytogenes, and Salmonella spp. were detected. Most of the B. cereus isolates identified were susceptible to penicillin, polymyxin B, ampicillin, oxacillin, and cefotaxime, whereas none of the isolates were positive for emetic toxin, an indication that cleansing procedure at the packaging sheds was not effective. Thorough sanitation and aseptic handling of fresh produce through out the packaging facilities is accentuated.