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Shan-Jing Yao,Yi-Xin Guan,Hai-Xue Pan,Yong-Gui Gao,Man-Gi Cho 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human interferon- (rhIFN-) at a high concentration. The rhIFN- was overexpressed in E. coli, resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded rhIFN-, with protein recovery of 67.1% and specific activity up to 1.2 107 IU/mg.
Luminescent characteristics of Sr2ZnSi2O7 : Eu3+ phosphor for ultraviolet light emitting diodes
Shan-shan Yao,Li-hong Xue,You-wei Yan,Yuan-yuan Li,Mi-fang Yan 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.6
Red-emitting phosphors Sr2-xZnSi2O7 : Eux 3+ was prepared by a combustion-assisted synthesis method and an efficient red emission under near-ultraviolet (UV) was observed. The luminescence, crystallinity, and particle sizes were investigated using luminescence spectrometry, X-ray diffractometry, and field scanning electron microscopy. The emission spectrum shows that the most intense peak is located at 614 nm, which corresponds to the 5D0→ 7F2 transition of Eu3+. The phosphor has two main excitation peaks located at 394 and 465 nm, which match the emission of UV and blue light-emitting diodes, respectively.
Yao Shan,Shunhua Zhou,Yao Shu 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.7
The effects of shield tunnel construction on the differential settlement and the distribution of soil dynamic stress of an adjacentculvert-embankment transition zone are investigated. A construction project of shield tunnels in Hangzhou (China) beneath anexisting railroad culvert-embankment transition zone is employed as a case study. Firstly, the shield tunneling activities in the vicinityof a culvert-embankment transition zone are simulated by a three-dimensional (3D) Finite Element Analysis (FEA) method. Thedifferential settlement of the transition zone is calculated to evaluate the influence of the shield tunneling on the safety of the passingtrain. Secondly, a plane strain model is employed to investigate the discipline of the soil dynamic stress in transition zones, which isinduced by the passing train and the shield tunnel beneath the railroad. Results indicate that the reinforcement treatment of thefoundation is required since the embankment differential settlement is significantly affected by the shield tunneling. Finally, arecommended treatment is introduced according to the property of the surrounding soil. Numerical simulation reveals that thistreatment is appropriate for reducing the differential settlement and soil dynamic stress of the transition zone.
Novel Architecture Design of Large-Scale Distributed Object Storage System
Shan Ying,YAO Nian-min 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.1
A novel architecture design of large-scale distributed object storage system (called DOSS) is proposed. Our design takes several aspects effecting on the overall performance of DOSS into consideration, including an improved model of interaction based on the traditional interactive mode of object-based storage systems, MDS(Metadata Server) management scheme and a load balancing scheme combines the definition of the maximum load for OSD(Object Storage Device), hierarchical model and re-polling in order to solve access of heat objects issues. Our experimental results show that the architecture is an effective way to promote the performance of DOSS.
Recent advances in carbon-fiber-reinforced thermoplastic composites: A review
Yao, Shan-Shan,Jin, Fan-Long,Rhee, Kyong Yop,Hui, David,Park, Soo-Jin Elsevier 2018 Composites Part B, Engineering Vol.142 No.-
<P><B>Abstract</B></P> <P>Carbon fibers (CFs) have high specific tensile strength, high modulus, and outstanding wear resistance, and are widely used for the reinforcement of advanced composite materials. CF-reinforced thermoplastic composites have received much attention because of their easy processability and recycling convenience compared with thermosetting composites. Surface treatment of CFs is generally employed to increase the surface functional groups and interfacial adhesion between the CFs and the surrounding polymer matrix. In this review, we explore recent advances in the surface treatment of CFs and preparation of CF/thermoplastic composites. The thermal, mechanical, and electrical properties of the composites are also discussed.</P>
Concentration quenching of Eu^2+ in Ba_2Mg(BO_3)_2: Eu^2+ phosphor
Shan-shan Yao,Li-hong Xue,You-wei Yan 한국물리학회 2011 Current Applied Physics Vol.11 No.3
The red emitting phosphor Ba_2Mg(BO_3)_2: Eu^2+ is prepared by the combustion-assisted method. An efficient red emission under conditions ranging from ultraviolet to visible light is observed. The emission spectrum shows a single intensive band centered at 610 nm, which correspond to the 4f^65d^1 → 4f^7transition of Eu^2+. The critical quenching concentration of Eu^2+ in Ba_2Mg(BO_3)_2: Eu^2+ phosphor is about 0.05 mol. The corresponding concentration quenching mechanism is verified to be a dipole―dipole interaction. The value of the critical transfer distance is calculated as 17.32 Å, which is in good agreement with the value (18 Å) derived from the experimental data.
Yao Shan-Shan,이슬이,Li Hai-Long,Jin Fan-Long,박수진 한국탄소학회 2024 Carbon Letters Vol.34 No.3
This study aimed to fabricate composites with high thermal conductivity using diglycidyl ether of bisphenol-A (DGEBA), incorporating carbon fiber cloth (CFC) and graphene as reinforcing agents. Notably, the dispersion of graphene within the DGEBA matrix was enhanced through surface modification via a silane coupling agent. The effects of CFC and graphene addition on the impact strength, thermal conductivity, and morphology of the composites were examined. The experimental results showed that the incorporation of 6 wt% CFC resulted in a substantial (16-fold) increase in impact strength. Furthermore, the introduction of 6 wt% CFCs along with 20 wt% graphene led to a remarkable enhancement in thermal conductivity to 5.7 W/(m K), which was approximately 22 and 4 times higher than the intrinsic thermal conductivities of pristine DGEBA and the CFC/DGEBA composite, respectively. The increased impact strength is ascribed to the incorporation of CFC and silane-modified graphene. Additionally, the gradual increase in thermal conductivity can be attributed to the enhanced interaction between the acidic silane-modified graphene and the basic epoxy–amine hardener within the system studied.
Shan-Shan Yao,Chun-Liu Ma,Fan-Long Jin,박수진 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.11
Graphene nanoplatelets (GNPs)/silane-coupling-agent-treated GNPs (KH- GNPs) and hydroxyl multi-walled carbon nanotubes (MWCNTs-OH) were added as reinforcing agents to an epoxy matrix, diglycidylether of bisphenol- A (DGEBA), to improve the fracture toughness of DGEBA. The influence of the MWCNT-OH fraction on the thermal and flexural properties, fracture toughness, and morphology of the DGEBA/GNP/MWCNT-OH and DGEBA/ KH-GNP/MWCNT-OH nanocomposites was investigated. The results indicate that the fracture toughness of the DGEBA/KH-GNP/MWCNT-OH nanocomposites increased from 1.09 to 1.46MPa·m1/2, which is 33.9% greater than that of pristine DGEBA. Analysis of the fracture surfaces of the nanocomposites showed a rough morphology with numerous tortuous and river-like structures. In addition, the MWCNTs-OH were slightly pulled out or broken in the epoxy matrix during the fracture toughness tests.