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Yun, Seok Joong,Jeong, Pildu,Kang, Ho Won,Kim, Ye-Hwan,Kim, Eun-Ah,Yan, Chunri,Choi, Young-Ki,Kim, Dongho,Kim, Jung Min,Kim, Seon-Kyu,Kim, Seon-Young,Kim, Sang Tae,Kim, Won Tae,Lee, Ok-Jun,Koh, Gou-Yo Korean Continence Society 2015 International Neurourology Journal Vol.19 No.2
<P><B>Purpose:</B></P><P>MicroRNAs (miRNAs) in biological fluids are potential biomarkers for the diagnosis and assessment of urological diseases such as benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The aim of the study was to identify and validate urinary cell-free miRNAs that can segregate patients with PCa from those with BPH. </P><P><B>Methods:</B></P><P>In total, 1,052 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. A urine-based miRNA microarray analysis suggested the presence of differentially expressed urinary miRNAs in patients with PCa, and these were further validated in three independent PCa cohorts, using a quantitative reverse transcriptionpolymerase chain reaction analysis. </P><P><B>Results:</B></P><P>The expression levels of hsa-miR-615-3p, hsv1-miR-H18, hsv2-miR-H9-5p, and hsa-miR-4316 were significantly higher in urine samples of patients with PCa than in those of BPH controls. In particular, herpes simplex virus (hsv)-derived hsv1-miR-H18 and hsv2-miR-H9-5p showed better diagnostic performance than did the serum prostate-specific antigen (PSA) test for patients in the PSA gray zone. Furthermore, a combination of urinary hsv2-miR-H9-5p with serum PSA showed high sensitivity and specificity, providing a potential clinical benefit by reducing unnecessary biopsies. </P><P><B>Conclusions:</B></P><P>Our findings showed that hsv-encoded hsv1-miR-H18 and hsv2-miR-H9-5p are significantly associated with PCa and can facilitate early diagnosis of PCa for patients within the serum PSA gray zone.</P>
( Seon Hwa Chung ),( Mi Kyung Kong ),( Eui Hyeok Kim ),( Sang Won Han ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.3
Objective The Bishop score and length of the uterine cervix are good predictors of successful labor induction. However, little is known about the association between the funneling of the uterine cervix and successful labor induction. The study aimed to evaluate cervical funneling as a predictor of successful labor induction. Methods This study was designed as a prospective observational study. Subjects who delivered a baby by labor induction were enrolled in the study from July 2011 to August 2013. Cervical funneling and length were examined with transvaginal ultrasonography. The Bishop score was rated by digital pelvic examination. Results A total of 163 primigravida women were recruited for the study. Of these, 137 participants (84.0%) delivered vaginally by labor induction. Cervical funneling was observed in 93 women (57.1%). Successful labor induction was significantly higher in patients with cervical funneling than those without it (91.4% vs. 74.3%, P<0.01), and was significantly associated with cervical funneling, as well as the Bishop score and cervical length. In a multivariate analysis, cervical funneling was an independent predictor for successful vaginal delivery by labor induction ( odd ratio, 2.70; 95% confidence interval, 1.02 to 7.10; P=0.04). However, the Bishop score and cervical length had no association with successful vaginal delivery. Conclusion This study showed that cervical funneling could be a predictive marker for vaginal delivery during labor induction.
A NOMOGRAM FOR PREDICTING AMYLOID PET POSITIVITY IN AMNESTIC MILD COGNITIVE IMPAIRMENT
Kim, Si Eun,Woo, Sook-Young,Kim, Seon Woo,Chin, Juhee,Kim, Hee Jin,Lee, Byung In,Park, JinSe,Park, Kyung Won,Noh, Young,Ye, Byoung Seok,Yoo, Han Soo,San Lee, Jin,Kim, Yeshin,Kim, Seung Joo,Cho, Soo Hy ELSEVIER SCIENCE B.V. AMSTERDAM 2018 ALZHEIMERS AND DEMENTIA Vol.14 No.7
Effects of Storage Buffer and Temperature on the Integrity of Human DNA
Kim, Yun-Tae,Choi, Eun-Hee,Son, Bo-Kyoung,Seo, Eun-Hee,Lee, Eun-Kyoung,Ryu, Je-Kwon,Ha, Gi-Won,Kim, Jin-Seon,Kwon, Mi-Ran,Nam, Jae-Hoon,Kim, Young-Jin,Lee, Kyoung-Ryul Korean Society for Clinical Laboratory Science 2012 대한임상검사과학회지(KJCLS) Vol.44 No.1
In this study, we have examined the effects of the storage time and temperature on DNA quality and have also studied the effects of the hydration buffer in which DNA is dissolved. This study was performed using 160 human blood samples collected with informed consent from 2007 to 2008 in the hospital where this cohort study was performed. The DNA extracted was dissolved using distilled water (DW) or Tris-EDTA (TE) buffer, and stored in the deep freezer or refrigerator for up to 10 weeks at $-70^{\circ}C$, $-20^{\circ}C$, $4^{\circ}C$, and $25^{\circ}C$, respectively. DNA integrity was determined by the degree of smearing of DNA on the gel. After four weeks, all of the 20 DNA samples dissolved in DW and stored at $25^{\circ}C$ were entirely degraded. After 10 weeks, 6 of the 20 DNA samples dissolved in TE buffer and stored at $25^{\circ}C$ were fairly degraded, and 4 of the 20 DNA samples dissolved in DW and stored at $4^{\circ}C$ were fairly degraded. The 20 DNA samples dissolved in TE buffer and stored at $4^{\circ}C$ were stable for 10 weeks. DNA samples stored at $-20^{\circ}C$ and $-70^{\circ}C$ did not appear to degrade in either DW or TE buffer, even at the 10-week point. We suggest that TE buffer should use for DNA elution, in order to protect against degradation and to preserve DNA for a long period of time, and the samples should be stored at $-20^{\circ}C$ or $-70^{\circ}C$.
Kim, Haeri,Moon, Seon Young,Kim, Shin-Ik,Baek, Seung-Hyub,Jang, Ho Won,Kim, Dong-Wook American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.16
<P>We investigated the influences of charge writing on the surface work function (<I>W</I>) and sheet resistance (<I>R</I>) of the LaAlO<SUB>3</SUB>/SrTiO<SUB>3</SUB> (LAO/STO) heterointerface in several gas environments: H<SUB>2</SUB>(2%)/N<SUB>2</SUB>(98%), air, N<SUB>2</SUB>, and O<SUB>2</SUB>. The decrease in <I>W</I> and <I>R</I> due to charge writing was much larger in air (Δ<I>W</I> = −0.45 eV and Δ<I>R</I> = −40 kΩ/<I>S</I>) than in O<SUB>2</SUB> (Δ<I>W</I> = −0.21 eV and Δ<I>R</I> = −19 kΩ/<I>S</I>). The reduced <I>R</I> could be maintained more than 100 h in H<SUB>2</SUB>/N<SUB>2</SUB>. Such distinct behaviors were quantitatively discussed, based on the proposed charge-writing mechanisms. Such analyses showed how several processes, such as carrier transfer via surface adsorbates, surface redox, electronic state modification, and electrochemical surface reactions, contributed to charge writing in each gas.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-16/am503367f/production/images/medium/am-2014-03367f_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am503367f'>ACS Electronic Supporting Info</A></P>
Kim, Seon-Hee,Park, Jin lI,Choi, Soon-Kyu,Kim, Ji-A,Kim, Si-Hwan,Kim, Jack C.,Park, Won-Woo ]東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.1
Ten, heretofore unreported, 5¹-methyl-5¹-[2-(5-substituted uracil-1-yl)ethyl)]-2¹-oxo-3¹-methylenetetrahydrofurans (H, F, Cl, Br, I, CH₃, CF₃, CH₂CH₃, CH=CH₂, SePh) (7a-j) were synthesized and evaluated against four cell lines (K-562, FM-3A, P-388 and U-937). For the preparation of α-methylene-γ-butyrolactone-linked to 5-substituted uracils (7a-j), the convenient Reformasky type reaction was employed which involves the treatment of ethyl α-(bromomethyl)acrylate and zinc with the respective 1-(5-substituted uracil-1-yl)-3-butanone (6a-j). The 5-substituted uracil ketones (6a-j) were directly obtained by the respective Michael type reaction of vinyl methyl ketone with the K₂CO₃(or NaH)-treated 5-substituted uracils (5a-j) in the presence of acetic acid in the DMF solvent. The α-methylene-γ-butyrolactone compounds showing the most significant antitumor activity are 7e, 7f, 7h and 7j (inhibitory concentration (IC) ranging from 0.69 to 2.9 ㎍/ml), while 7b, 7g and 7i have shown moderate to significant activity. The compounds 7a, 7c and 7d were found to be inactive. The synthetic intermediate compounds 6a-j were also screened and found marginal to moderate activity where compounds 6b and 6g showed significant activity (IC:0.4∼2.8 ㎍/ml).