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Lim, Sanghyun,Lee, Kukjoo,Shin, Inseop,Tron, Artur,Mun, Junyoung,Yim, Taeeun,Kim, Tae-Hyun Elsevier Sequoia 2017 Journal of Power Sources Vol. No.
<P><B>Abstract</B></P> <P>The practical applications of Si electrodes in lithium-ion batteries are limited since they undergo large changes in volume during charge and discharge, and consequently become highly deteriorated. A novel binder system holding silicon particles together and preventing disintegration of the electrode during operation hence needs to be developed to enable reliable cycleability. In the current work, such a new polymer binder system, based on poly(acrylic acid) (PAA) and poly(ethylene glycol-<I>co</I>-benzimidazole) (PEGPBI), is developed for silicon anodes. The physical crosslinking using acid-base interactions between PAA and PBI, together with the ion-conducting PEG group, yields physical properties for the resulting PAA-PEGPBI-based anodes that are better than those of electrodes based on the currently available PAA binder, and yields good cell performances. A Si-based electrode with high loading levels of 1.0–1.3 mg cm<SUP>−2</SUP> (0.7–0.91 Si mg cm<SUP>−2</SUP>) is reliably manufactured using specifically PAA-PEGPBI-2, which is made with 2 wt% of PEGPBI relative to PAA, and shows a very high capacity value of 1221 mAh g<SUP>−1</SUP> at a rate of 0.5 C after 50 cycles, and a high capacity value of more than 1600 mAh g<SUP>−1</SUP> at a high rate of 2 C.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Physically-crosslinked polymer binders are developed for Si anode. </LI> <LI> Physical and electrochemical properties of new binders are investigated. </LI> <LI> Reversible acid-base interaction and ion conductivity are provided to new binders. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Development of SNP markers associated with citrus canker
Sanghyun Lim,Seunghee Ko,Young Chul Park,Yoon Kyung Uhm,Jae Joon Kim,Kwan Jeong Song,Ho Bang Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Citrus canker caused by Xanthomonas citri is a notorious disease affecting a decrease in fruit productivity and quality. Citrus export to USA is also prohibited by the disease. Therefore, development of citrus canker resistant variety is essential and exploitation of markers for molecular breeding is urgent. To develop DNA molecular markers, we performed whole genome resequencing for 8 varieties: 4 citrus canker resistant varieties including C. hybrid ‘Kioymi’ and 4 citrus canker susceptible varieties including C. iyo ‘Miyauchiiyokan’. In total, 642 polymorphic SNPs were detected between resistant and susceptible varieties. Of the 642 SNPs, 50 SNPs were preferably selected based on integrative genomics viewer. To apply the markers in a broad range of citrus variety, we performed genotyping with 6 other varieties very well known as citrus canker resistant and susceptible varieties in addition to previous mentioned 8 varieties. Three of the 50 SNPs were identified as a marker to distinguish citrus canker resistant varieties from susceptible varieties. Secondly, we developed molecular markers to apply for F1 lines crossed by ‘Kiyomi’ and ‘Miyauchiiyokan’. Of the 50 SNPs, we identified 2 SNP markers to distinguish between F1 resistant and susceptible lines. One of them is a resistance gene that plays a role in plant defense mechanism. In this study, we developed 5 molecular marker candidates possible to apply for molecular breeding to develop citrus canker resistant variety. We are working on development of candidate markers related to citrus canker.
Effects of multi-species probiotic supplementation on alcohol metabolism in rats
Lim Tae-Joong,Lim Sanghyun,Yoon Jong Hyun,Chung Myung Jun 한국미생물학회 2021 The journal of microbiology Vol.59 No.4
Probiotics are known to protect against liver damage induced by the alcohol and acetaldehyde accumulation associated with alcohol intake. However, there have been few studies of the direct effect of probiotics on alcohol metabolism, and the types of probiotics that were previously analyzed were few in number. Here, we investigated the effects of 19 probiotic species on alcohol and acetaldehyde metabolism. Four probiotic species that had a relatively high tolerance to alcohol and metabolized alcohol and acetaldehyde effectively were identified: Lactobacillus gasseri CBT LGA1, Lactobacillus casei CBT LC5, Bifidobacterium lactis CBT BL3, and Bifidobacterium breve CBT BR3. These species also demonstrated high mRNA expression of alcohol and acetaldehyde dehydrogenases. Pro- AP4, a mixture of these four probiotics species and excipient, was then administered to rats for 2 weeks in advance of acute alcohol administration. The serum alcohol and acetaldehyde concentrations were significantly lower in the ProAP4-administered group than in the control and excipient groups. Thus, the administration of ProAP4, containing four probiotic species, quickly lowers blood alcohol and acetaldehyde concentrations in an alcohol and acetaldehyde dehydrogenasedependent manner. Furthermore, the serum alanine aminotransferase activity, which is indicative of liver damage, was significantly lower in the ProAP4 group than in the control group. The present findings suggest that ProAP4 may be an effective means of limiting alcohol-induced liver damage.
Genome-wide analysis of structural variation in citrus two varieties related to citrus canker
Sanghyun Lim,Seunghee Ko,Young Chul Park,Jae Joon Kim,Yoon Kyung Uhm,Yong-Chull Jeun,Kwan Jeong Song,Ho Bang Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Citrus canker caused by Xanthomonas citri pv. citri is one of economically important diseases in the citrus industry. The devastating bacterial disease results in unattractive quality and a significant reduction in fruit production. Citrus growers and industry in Korea has been struggling with the serious disease causing the prohibition of export market. Korea also became the top import market for oranges. The development of markers linked to citrus canker resistance is strongly needed. In this study, we investigated molecular markers between ‘Kiyomi’ (Citrus unshiu x C. sinensis), a resistant cultivar, and Natsudaidai (C. natsudaidai), a susceptible cultivar. To develop markers, we focused on structural variation (copy number variation, CNV, and presence/absence variation, PAV). It has been well documented that CNV and PAV of defense-related genes are associated with resistant cultivars. Using a read depth approach following next-generation sequencing, we performed genome-wide analysis of CNV and PAV in two varieties. As a result, 633 genes showing at least two times difference between the mapping reads from two varieties and 61 genes showing presence of the mapping reads in only either one of them were screened. Visual inspection using the Integrative Genomics Viewer (IGV) was performed and experimental validation is being investigated. Interestingly, one of PAV candidates showed polymorphism in ‘Kiyomi’ and ‘Natsudaidai’ as well as other resistant and susceptible cultivars. Our results suggest a necessity for the detection of structural variation and indicate that the candidates may be useful for molecular breeding for citrus canker resistance and understanding disease resistance mechanism.
Lim, Sanghyun,Chu, Hodong,Lee, Kukjoo,Yim, Taeeun,Kim, Young-Jun,Mun, Junyoung,Kim, Tae-Hyun American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.42
<P>Silicon is greatly promising for high-capacity anode materials in lithium-ion batteries (LIBs) due to their exceptionally high theoretical capacity. However, it has a big challenge of severe volume changes during charge and discharge, resulting in substantial deterioration of the electrode and restricting its practical application. This conflict requires a novel binder system enabling reliable cyclability to hold silicon particles without severe disintegration of the electrode. Here, a physically cross-linked polymer binder induced by reversible acid–base interaction is reported for high performance silicon-anodes. Chemical cross-linking of polymer binders, mainly based on acidic polymers including poly(acrylic acid) (PAA), have been suggested as effective ways to accommodate the volume expansion of Si-based electrodes. Unlike the common chemical cross-linking, which causes a gradual and nonreversible fracturing of the cross-linked network, a physically cross-linked binder based on PAA–PBI (poly(benzimidazole)) efficiently holds the Si particles even after the large volume changes due to its ability to reversibly reconstruct ionic bonds. The PBI-containing binder, PAA–PBI-2, exhibited large capacity (1376.7 mAh g<SUP>–1</SUP>), high Coulombic efficiency (99.1%) and excellent cyclability (751.0 mAh g<SUP>–1</SUP> after 100 cycles). This simple yet efficient method is promising to solve the failures relating with pulverization and isolation from the severe volume changes of the Si electrode, and advance the realization of high-capacity LIBs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-42/acsami.5b06682/production/images/medium/am-2015-06682h_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b06682'>ACS Electronic Supporting Info</A></P>
Three new records of the subfamily Epyrinae Hymenoptera: Bethylidae) from Korea
Jongok LIM,Hyunjeong KWON,Jongwook LEE,Sanghyun KOH,Seunghwan LEE 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
Three species, Allobethylus tomoae Terayama, Cephalonomia tarsalis (Ashmead), and Laelius yamatonis Terayama of the subfamily Epyrinae are recorded for the first time in Korea. Both sexes of A. tomoae Terayama, C. tarsalis (Ashmead) and female of L. yamatonis Terayama are briefly described with diagnostic morphological illustrations for each species based on the Korean specimens. Male of the genus Allobethylus is discovered for the first time from the word.