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Sung Min Bae,Tae Young Shin,Young Soon Jun,Seung Hee Lee,Won Seok Kwak,Yong Oh Ahn,Dong Jun Kim,See Nae Lee,In Hee Kim,Soo Dong Woo 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
Insects are among the most diverse groups of animals on the planet, representing more than half of all known living organisms. These insects are found in nearly every environment. Although humans regard certain insects as pests and attempt to control them using insecticides, most insects perform complex ecological functions, and provide either direct or indirect economic benefits to humans. Recently, the importance of insects used as food sources or as pets has increased in many countries, including Korea. In addition, several insects have a strong influence on people's emotion. Insect-mediated mental healthcare program is designed to help people who have disorders with physical, behavior and development. Children who have mental disorder, the experimental group that was provided with an insect-mediated mental healthcare program over a total of 8 sections, one section per week, 60 minutes per section, followed by pre-test and post-test. They responded to therapeutic effect after the completion of the program. Further research on the basis of this study is expected to help children with emotional therapy in other areas.
Bae, Mi-Ok,Choi, Kyung-Ho,Lee, Hu-Jang,Kim, Hyun-Woo,Kim, Jun-Sung,Hwang, Soon-Kyung,Park, Jin-Hong,Cho, Hyun-Sun,Cho, Myung-Haing The Korean Society of Veterinary Science 2004 大韓獸醫學會誌 Vol.44 No.3
When an organism is exposed to various toxicants chronically, reactive oxygen species(ROS) are accumulated and eventually result in several biological effects from gene expression to cell death. In the present study we investigated the oxidative damage of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) and/or benzo(a)pyrene (B(a)P) in C100 cells. C100 cells treated with TCDD(30 nM) and B(a)P($3{\mu}M$) underwent diverse oxidative stress as determined through thiobarbituric acid-reactive substances(TBARS) formation, DNA fragmentation, DNA single strand break(SSB) assay, immunohistochemical staining of 8-hydroxy-2'-deoxyguanosine(8-OHdG), and mRNA expressions of antioxidant enzymatic genes such as Cu/Zn-SOD gene, GPx(glutathione peroxidase 5) gene, and catalase gene. Lipid peroxidation in C100 cells was determined through measuing the formation of TBARS. For theat, the cells were pretreated with TCDD(30 nM) and/or B(a)P($3{\mu}M$) for 0.5, 1, 2 and 4 days. TBARS formation was increased in TCDD(30 nM) and B(a)P($3{\mu}M$) and mixture($30nM\;TCDD+3{\mu}M\;B(a)P$) and positive control treatment groups comparing to the controls. Mixture treatment induced more DNA fragmentation than the single treatment group at day 6. Also, SSB in all treatment groups was clearly observed when compared with the negative control group. As with the expression of antioxidant enzyme, GPx 5mRNA, B(a)P alone and mixture($30nM\;TCDD+3{\mu}M\;B(a)P$) treatment were higher comparing to those of the negative control and TCDD treatment groups. Our results suggest that exposure of C100 cells to mixture of TCDD and B(a)P leads to significant oxidative damage comparing to the exposures to the individual chemicals. Mechanisms of action are discussed. Additional studies are needed to elucidate the detailed mechanism of mixture-induced toxicity.
Bae, Woo Yong,Kim, Lee Suk,Hur, Dae Young,Jeong, Sung Wook,Kim, Jae Ryong The American Laryngological, Rhinological Otologic 2008 The Laryngoscope Vol.118 No.9
OBJECTIVES/HYPOTHESIS:: Hair cell loss results in the secondary loss of spiral ganglion neurons (SGNs), over a period of several weeks. The death of the SGNs themselves results from apoptosis. Previous studies have shown that several molecules are involved in the apoptosis of SGNs that occurred secondary to hair cell loss. However, the precise mechanism of apoptosis of the SGNs remains unclear. The aim of this study was to ascertain the secondary apoptosis of spiral ganglion cells induced by aminoglycoside and to investigate the role of the Fas–FasL signaling pathway using guinea pigs as an experimental animal model. STUDY DESIGN:: Laboratory study using experimental animals. METHODS:: Guinea pigs weighing 250 to 300 g (n = 21) from 3 to 4 weeks of age were used. Gentamicin (60 μL) was injected through a cochleostomy site on their left side. At 1 (n = 7), 2 (n = 7), and 3 (n = 7) weeks after gentamicin treatment, their cochleas were obtained from their temporal bone. Hematoxylin and eosin and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling staining were performed to observe apoptosis. To investigate the involvement of the Fas–FasL signaling pathway in the secondary apoptosis of SGNs, we performed reverse transcription-polymerase chain reaction (RT-PCR), western blotting, and immunohistochemistry. RESULTS:: A progressive loss of spiral ganglion cells with increasing time after gentamicin treatment was observed on light microscopic examination. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling staining demonstrated induction of apoptotic cell death in SGNs after gentamicin treatment. Expression of FasL increased over time after gentamicin treatment as determined by RT-PCR and western blotting. On immunohistochemical staining, we observed the localization of FasL in the SGNs. The proapoptotic molecules Bax and Bad were increased, but levels of the antiapoptotic molecule Bcl-2 were decreased at increasing survival times after gentamicin treatment on RT-PCR. The gentamicin-treated group displayed initial activation of caspase-8 and increased the cleavage of caspase-3, caspase-8, and PARP protein in a time-dependent manner. CONCLUSIONS:: The secondary apoptosis of SGNs could be a result of the apoptotic Fas–FasL signaling pathway. Blocking the Fas–FasL signaling pathway could be considered as a method for preventing secondary degeneration of SGNs, and further studies are needed to confirm this.
( Sung Soo La ),( Dong Woo Kim ),( Ki Chul Shin ),( Se Young Yun ),( Suk Bae Kim ),( Jung Eun Shin ),( Hong Ja Kim ),( Il Han Song ) 대한소화기학회 2007 SIDDS Vol.9 No.-
Background/Aims: Serum alanine aminotransferase (ALT) activity, reflecting the degree of hepatic necroinflammation, is one of the most frequently measured liver tests in persons who are suspected to have liver disease. But current upper limit of normal (ULN) of ALT, 40 IU/L, often can not detect the subclinical liver disease with minimal to mild inflammation. We, therefore, evaluated the ULN of ALT of healthy adult subjects in order to assess the necessity of revision for currently accepted reference range of ALT. Methods: 23,898 adult subjects who visited for medical examination at Health Promotion Center, Dankook University Hospital, from January 2005 to December 2006, were retrospectively enrolled in the present study. We excluded the adults who had HBsAg, anti-HCV, anti-HIV, VDRL, history of current medication and alcohol consumption more than 40 g/week, and high risks for liver disease defined as BMI more than 23 kg/m2, serum cholesterol level more than 240 mg/dL, triglyceride more than 200 mg/dL, fasting glucose more than 115 mg/dL, and ultrasonographic liver diseases including fatty change. We defined updated ULN of ALT as activity below 95th percentile for each gender. Results: 5,994 adults (2,983 men, 2,961 women) met the inclusion criteria of this present study. Updated ULN of ALT levels were 38 IU/L in men and 25 IU/L in women. This ALT activity showed a tendency to increase according to age increase, and revealed positive correlation with the ultrasonographic degree of fatty liver. Serum ALT activity below 95th percentile of HBsAg or anti-HCV positive carrier subjects, whose medical conditions were equal to those of enrolled healthy subjects in aspect of biochemical and ultrasonographic finding, were 75 IU/L and 77 IU/L, respectively. Conclusions: Updated ULN of ALT was lower than currently accepted ALT activity. Considering age and gender, current reference range of serum ALT level should be revised for the detection of subclinical liver diseases such as fatty liver.
Structure-related Characteristics of SiGe HBT and 2.4 ㎓ Down-conversion Mixer
Sang-Heung Lee,Sang-Hoon Kim,Ja-Yol Lee,Hyun-Cheol Bae,Seung-Yun Lee,Jin-Yeong Kang,Bo Woo Kim 대한전자공학회 2006 Journal of semiconductor technology and science Vol.6 No.2
In this paper, the effect of base and collector structures on DC, small signal characteristics of SiGe HBTs fabricated by RPCVD was investigated. The structure of SiGe HBTs was designed into four types as follows: SiGe HBT structures which are standard, apply extrinsic-base SEG selective epitaxial growth (SEG), apply selective collector implantation (SCI), and apply both extrinsic-base SEG and SCI. We verified the devices could be applied to the fabrication of RFIC chip through a fully integrated 2.4 ㎓ down-conversion mixer.