Plant Regeneration and Bulblet Formation of Allium wakegi Araki

Song,Won-seob,Yang,Deok-Chun,Yoon,Jae-Ho,Ryu,Sang-Hyun 한국자원식물학회 2004 Plant Resources Vol.7 No.1

Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

Identification of the Gene Products Responsible for F Plasmid Partitioning

KIM, SUNG0UK,NAGAI, KAZUO,TAMURA, GAKUZO,YU, JU-HYUN,BOK, SONG-HAE 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.4

DNA subfragments, sopA, sopB and sopC which help to maintain the stability of an on C plasmid, were derived from a mini-F plasmid DNA (EcoRI restriction fragment f5) after digestion with restriction endonuclease, and cloned in the vector plasmid pBR322. The recombinant plasmids obtained were introduced into E. coli KY7231 and E. coli CSR603 strains, and proteins specified by the mini-F fragments were analysed by SDS-PAGE. Two proteins encoded by the F fragments were detected, and their molecular weights were 41,000 and 37,000 daltons. Fluorography after one and two dimensional gel electrophoresis of the lysates showed that these two proteins had been overproduced in the cells which were allowed to incorporate radioactive amino acid after plasmid amplification by chloramphenicol treatment. The isoelectric points of sopA and sopB proteins were 6.6 and 7.0, respectively.

Quantitative determination of aminopyrine in pig kidney tissues by surrogate analyte-based LC-MS/MS method

Hyun ju Han,Hyo bi Kim,Soo hee Kim,Jae Young Song,Sung Won Park 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-

Gamma-Ray Susceptibility of Immature and Mature Hippocampal Cultured Cells

SONG, Myoung-Sub,KIM, Joong-Sun,YANG, Miyoung,KIM, Sung-Ho,KIM, Jong-Choon,PARK, Soo Hyun,SHIN, Taekyun,MOON, Changjong Japanese Society of Veterinary Science 2010 The Journal of veterinary medical science Vol.72 No.5

<P>Ionizing radiation suppresses neurogenesis in the mammalian brain. This <I>in vitro</I> study compared the detrimental effect of acute gamma-irradiation on immature hippocampal cells with mature cells. Both rat immature (0.5 day <I>in vitro</I> (DIV)) and mature hippocampal cells (14 DIV) were irradiated with 0-4 Gy gamma-rays. Cell viability was analyzed by using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. DNA fragmentation study was performed by extracting intracellular DNA. Morphological features of apoptosis were characterized by 4',6-diamidine-2'-phenylindole, dihydrochloride (DAPI) staining. MTT assay revealed that the survival rate of immature hippocampal cells declined in a dose-dependent manner within the range of irradiation applied, but was not changed in mature cells. Intranucleosomal DNA fragmentation in a ladder like pattern was dose-dependently increased in immature cells, but not in mature cells. The number of apoptotic nuclei in immature cells increased significantly in a dose-dependent manner within the range of irradiation applied. Active caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP) expressions in immature hippocampal cells at 6 hr after 2 Gy exposure were markedly higher than control levels. The significantly greater radiosensitivity of immature hippocampal cells than that of the mature cells, indicates that the susceptibility of such hippocampal cells depends on their maturation. In addition, gamma-irradiation may induce caspase-dependent apoptosis in immature hippocampal cells.</P>

Post-activation treatment with demecolcine improves development of somatic cell nuclear transfer embryos in pigs by modifying the remodeling of donor nuclei

Song, Kilyoung,Hyun, Sang-Hwan,Shin, Taeyoung,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2009 Molecular reproduction and development Vol.76 No.7

<P>The objective of this study was to examine the effect of cytochalasin B (CB) and/or demecolcine (Dc) on the remodeling of donor nuclei, nuclear ploidy, and development of somatic cell nuclear transfer (SCNT) and parthenogenetic (PA) pig embryos. SCNT and PA oocytes were either untreated (control), or treated with CB, Dc, or both CB and Dc after electric activation, and then cultured or transferred to surrogates. In SCNT, blastocyst formation was higher after treatment with CB and/or Dc (26–28%) than in the controls (16%). The number of oocytes that formed a single pronucleus (PN) was higher after treatment with Dc (86%) and CB + Dc (86%) than under control conditions (44%) or after treatment with CB (63%). In PA, blastocyst formation was higher after CB treatment (47%) than under control conditions (28%), while the formation of a single PN was higher after treatment with Dc (88%) and CB + Dc (84%) compared to controls (34%). The rate of formation of diploid embryos was higher after treatment with Dc and CB + Dc than under control conditions. Dc treatment resulted in a farrowing rate of 50% with 1.1% production efficiency, while controls showed a farrowing rate of 37.5% and a production efficiency of 0.7%. The results of our study demonstrate that post-activation treatment with Dc improves preimplantation development and supports normal in vivo development of SCNT pig embryos, probably because Dc induces formation of a single PN and this leads to normal nuclear ploidy. Mol. Reprod. Dev. 76: 611–619, 2009. © 2008 Wiley-Liss, Inc.</P>

Is the early cyclosporine A level predictive of the outcome of immunosuppressive therapy in severe aplastic anemia?

Song, Moo-Kon,Chung, Joo-Seop,Joo, Young-Don,Kim, Yang-Soo,Kim, Sung-Hyun,Seol, Young-Mi,Shin, Ho-Jin,Choi, Young-Jin,Cho, Goon-Jae Blackwell Publishing Ltd 2009 European journal of haematology Vol.83 No.1

<P>Abstract</P><P>Immunosuppressive therapy (IST) has provided an alternative treatment option for cure of aplastic anemia patients who cannot receive bone marrow transplantation. Although there have been many recent studies on the efficacy of antithymoglobulin (ATG) combined with cyclosporine A (CsA), there is no data on the correlation between the variability of CsA levels and the response to IST. Therefore, we retrospectively assessed the factors associated with IST efficacy in patients with acquired severe aplastic anemia (SAA). Sixty-six patients were treated with ATG combined with CsA for 6 months. In the response group, the CsA levels were increased rapidly to more than 200 ng/mL within the first 2 wk after starting the IST. However, the non-response group had a pattern of slower increase of the CsA levels. The CsA levels, during the first and second week of treatment with IST, were significantly different in the responders and non-responders. The factors predictive of response to IST and survival were analyzed. The univariate analysis showed that a younger age at the initiation of IST, a high absolute neutrophil count prior to starting IST, a short interval between the diagnosis and initiation of IST, and high CsA levels during the first and second week of IST treatment were positively associated with the response rate and overall survival. The multivariate analysis showed that these four factors were independent factors associated with a longer patient survival. A high response rate was associated with a short interval between diagnosis and initiation of IST as well as high CsA levels during the first and second week of IST. Therefore, early intensification of CsA levels might improve patient outcome.</P>

Metal-directed supramolecular assembly of metal(II) benzoates (M=Co, Ni, Cu, Zn, Mn, and Cd) with 4,4′-bipyridine: Effects of metal coordination modes and novel catalytic activities

Song, Young Joo,Kwak, Han,Lee, Young Min,Kim, Soo Hyun,Lee, Sun Hwa,Park, Byeong Kwon,Jun, Je Yeol,Yu, Seung Man,Kim, Cheal,Kim, Sung-Jin,Kim, Youngmee Elsevier 2009 Polyhedron Vol.28 No.7

<P><B>Graphical abstract</B></P><P>We have presented a systematic investigation on the coordination polymers assembled from metal benzoates and 4,4′-bpy. Since different structures provide different coordination geometry of each metal ion, it is clear that selection of appropriate metal ions can control the coordination geometry of each metal ion to form different crystal structures. Reactivity study of seven compounds for the transesterification of a variety of esters has shown that <B>4</B>-Zn and <B>5</B>-Zn are very efficient and the best among them. It can be possible to tune the catalytic activities by changing from Zn to those metals such as Cd, a kinetically inert metal, or Cu, Mn, and Co, the redox-active metals.</P><ce:figure></ce:figure> <P><B>Abstract</B></P><P>Six polymeric metal(II)-benzoate complexes of formula [Co<SUB>2</SUB>(O<SUB>2</SUB>CPh)<SUB>4</SUB>(4,4′-bpy)<SUB>2</SUB>]<I><SUB>n</SUB></I> (<B>1</B>-Co), [Ni(O<SUB>2</SUB>CPh)<SUB>4</SUB>(H<SUB>2</SUB>O)<SUB>2</SUB>(4,4′-bpy)]<I><SUB>n</SUB></I> (<B>2</B>-Ni), [Cu<SUB>2</SUB>(O<SUB>2</SUB>CPh)<SUB>4</SUB>(4,4′-bpy)]<I><SUB>n</SUB></I> (<B>3</B>-Cu), [Zn<SUB>2</SUB>(O<SUB>2</SUB>CPh)<SUB>2</SUB>(OH)<SUB>2</SUB>(4,4′-bpy)<SUB>2</SUB>]<I><SUB>n</SUB></I> (<B>4</B>-Zn), [Zn<SUB>3</SUB>(O<SUB>2</SUB>CPh)<SUB>4</SUB>(μ-OH)<SUB>2</SUB>(4,4′-bpy)<SUB>2</SUB>]<I><SUB>n</SUB></I> (<B>5</B>-Zn), and [Cd<SUB>2</SUB>(O<SUB>2</SUB>CPh)<SUB>4</SUB>(4,4′-bpy)<SUB>2</SUB>]<I><SUB>n</SUB></I> (<B>6</B>-Cd) have been synthesized and characterized (4,4′-bpy=4,4′-bipyridine). <B>1</B>-Co and <B>6</B>-Cd show ladder-type double chains, <B>2</B>-Ni does a helical structure, <B>3</B>-Cu does a one-dimensional chain containing paddle-wheel units, <B>4</B>-Zn does a zigzag chain, and <B>5</B>-Zn does two-dimensional sheets. Since different structures provide different coordination geometry of each metal ion, it is clear that selection of appropriate metal ions can control the coordination geometry of each metal ion to form different crystal structures. Reactivity study of the compounds <B>1</B>–<B>7</B> for the transesterification of a variety of esters has shown that <B>4</B>-Zn and <B>5</B>-Zn are very efficient and the best among them. The catalyst <B>6</B>-Cd containing Cd ion, well known as an inert metal ion for the ligand substitution, also catalyzed efficiently the transesterification of a variety of esters, and its reactivity is comparable to <B>4</B>-Zn and <B>5</B>-Zn. Moreover, the redox-active metal-containing polymers, <B>1</B>-Co, <B>3</B>-Cu, and <B>7</B>-Mn, have shown efficient catalytic reactivities for the transesterification reactions, while <B>2</B>-Ni has displayed a very slow conversion. The reactivities of the compounds used in this study are in the order of <B>5</B>-Zn><B>4</B>-Zn><B>6</B>-Cd><B>7</B>-Mn∼<B>3</B>-Cu><B>1</B>-Co><B>2</B>-Ni, indicating that the non-redox metal-containing compounds (<B>5</B>-Zn, <B>4</B>-Zn, and <B>6</B>-Cd) show better activity than the redox-active metal-containing compounds (<B>7</B>-Mn, <B>3</B>-Cu, <B>1</B>-Co, and <B>2</B>-Ni). These results suggest that it is possible to tune the catalytic activities by changing from Zn to those metals such as Cd, a kinetically inert metal, or Cu, Mn, and Co, the redox-active metals.</P>

Expression, Solubilization and Purification of a Human Olfactory Receptor from Escherichia coli

Song, Hyun Seok,Lee, Sang Hun,Oh, Eun Hae,Park, Tai Hyun Springer-Verlag 2009 Current microbiology Vol.59 No.3

A New Angle-Based Location Method Using a Forward-Link Signal

Song, Seung-Hun,Im, Hyun-Ja,Park, Ji-Won,Sung, Tae-Kyung Hindawi Publishing Corporation 2009 EURASIP journal on advances in signal processing Vol.2009 No.1

<P>With conventional AOA positioning using a reverse-link signal, each sensor is equipped with an array antenna to measure the incident angle of the signal emitting by a mobile source. In order to perform the complicated array processing for angle measurements, both the sensor size and the power consumed by the sensor can increase in RLAOA (reverse-link AOA) positioning. In some applications such as mobile vehicle localization, the vehicle has fewer limitations in terms of size or power consumption. Rather, it is desirable to make the sensor as light as possible. This paper presents a new angle-based positioning scheme using a forward-link signal. Under the assumption that a ground vehicle moves on a horizontal surface, a measurement equation for the FLAOA (forward-link AOA) is initially derived. Using the measurement equation, a closed-form solution for FLAOA positioning is proposed. With the proposed method, it is also possible to estimate the azimuth of the vehicle as well as its position. The performance of the proposed method is compared to that of RLAOA positioning in a computer simulation. The simulation results show that the proposed method is potentially suitable for applications involving the localization and guidance of mobile vehicles.</P>

Enhanced invasiveness of drug-resistant acute myeloid leukemia cells through increased expression of matrix metalloproteinase-2

Song, Ju Han,Kim, Seung Hyun,Cho, Daeho,Lee, Il-Kwon,Kim, Hyeoung-Joon,Kim, Tae Sung Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.125 No.5

<P>The acquired drug resistance as well as extramedullary tissue infiltration of leukemic cells is a major obstacle in leukemia treatment. Excessive egress of leukemia cell blasts results in invasion into various organs or tissues, which is facilitated by the catalytic activities of matrix metalloproteinases (MMPs). However, the migration of chemoresistant leukemia cells remains unclear. Here, we generated drug-resistant variants of the human acute myeloid leukemia cell line (AML-2/WT) by stepwise exposure to anticancer drugs and evaluated the level of MMP-2 in the drug-resistant variants, along with their invasiveness. Each of the drug-resistant cell variants demonstrated predominant increases in the expression and gelatinolytic activity of MMP-2 as well as in invasiveness, which were significantly suppressed by both a MMP-2 inhibitor and a blocking antibody. Knockdown experiments using MMP-2 short hairpin RNA also indicated that its upregulation was strongly associated with the cells' increased invasive properties. Importantly, elevated levels of MMP-2 activity and invasiveness were observed in ex vivo mononuclear cell of bone marrow from patients with poor responses to chemotherapy. These findings suggest that advanced malignancy due to acquired drug resistance is responsible for the progressive invasiveness of leukemia cells via MMP-2. © 2009 UICC</P>