Electrochemical synthesis of a novel poly(2,5-dimethoxy aniline) nanorod for ultrasensitive glucose biosensor application

D. Ragupathy,이수출,Salem S Al-Deyab,A. Rajendren 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.3

We report for the first time a rapid electrochemical synthesis of one-dimensional poly(2,5-dimethoxyaniline) nanorods (PDMA-NR) in the presence of surfactant. FE-SEM and TEM imagesconfirm the PDMA-NR formation and the average diameter of single rod sizes in the range of ~200–300 nm. An enzymatic glucose biosensor was fabricated through immobilizing glucose oxidase (GOx)into PDMA-NR matrix. The amperometric current response of PDMA-NR/GOx to glucose is linear in theconcentration range between 1 and 10 mM with a detection limit of 0.5 mM (S/N = 3). The PDMA-NR/GOxelectrode possesses high sensitivity (5.03 mA/mM), selectivity, stability, and reproducibility towardglucose.

Preparation of nylon-6/chitosan composites by nanospider technology and their use as candidate for antibacterial agents

Mohamed Hassan El-Newehy,Salem Slayyem Al-Deyab,Rajkumar Nirmala,Ahmed Abdel-Megeed,Hak Yong Kim 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.2

Electrospun nylon-6/chitosan (nylon-6/Ch) nanofibers were prepared by nanospider technology. Quaternary ammonium salts as antibacterial agent were immobilized onto electrospun nylon-6/Ch nanofibers via surface modification by soaking the mat in aqueous solution of glycidyltrimethylammonium chloride (GTMAC) at room temperature overnight to give nylon-6/N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (nylon-6/HTCC). The morphological,structural and thermal properties of the nylon-6/ch nanofibers were studied by field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, and thermogravimetric analysis (TGA). Biological screening has demonstrated the antibacterial activity of the electrospun nanofibers against Gram negative bacteria, Escherichia coli 35218, and Pseudomonas aeruginosa and Gram positive bacteria,Staphylococcus aureus 24213 among the tested microbes. Thus, the study ascertains the value of the use of electrospun nanofibers, which could be of considerable interest to the development of new antibacterial materials for biomedical applications.

Simple and rapid synthesis of polyaniline microrods and its electrical properties

D. Ragupathy,이수출,Salem S Al-Deyab,A. Rajendran 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.4

We report a facile and surfactant assisted synthesis of polyaniline microrods (PANI-MR) by ‘‘single-step chemical oxidative polymerization’’ method. The morphology of the PANI-MR was investigated by fieldemission scanning electron microscopy (FE-SEM). The average diameter of single PANI-MR was calculated to be 1.3 mm. The physiochemical characterization was examined using UV–visible spectroscopy, Fourier transform infrared spectroscopy and cyclic voltammetry. The electrical conducting property of the PANI-MR was analyzed using four-probe measurement method.

Cobalt/copper-decorated carbon nanofibers as novel non-precious electrocatalyst for methanol electrooxidation

Barakat, Nasser A M,El-Newehy, Mohamed,Al-Deyab, Salem S,Kim, Hak Yong Springer 2014 NANOSCALE RESEARCH LETTERS Vol.9 No.1

<P>In this study, Co/Cu-decorated carbon nanofibers are introduced as novel electrocatalyst for methanol oxidation. The introduced nanofibers have been prepared based on graphitization of poly(vinyl alcohol) which has high carbon content compared to many polymer precursors for carbon nanofiber synthesis. Typically, calcination in argon atmosphere of electrospun nanofibers composed of cobalt acetate tetrahydrate, copper acetate monohydrate, and poly(vinyl alcohol) leads to form carbon nanofibers decorated by CoCu nanoparticles. The graphitization of the poly(vinyl alcohol) has been enhanced due to presence of cobalt which acts as effective catalyst. The physicochemical characterization affirmed that the metallic nanoparticles are sheathed by thin crystalline graphite layer. Investigation of the electrocatalytic activity of the introduced nanofibers toward methanol oxidation indicates good performance, as the corresponding onset potential was small compared to many reported materials; 310 mV (vs. Ag/AgCl electrode) and a current density of 12 mA/cm<SUP>2</SUP> was obtained. Moreover, due to the graphite shield, good stability was observed. Overall, the introduced study opens new avenue for cheap and stable transition metals-based nanostructures as non-precious catalysts for fuel cell applications.</P>

Influence of Capsaicin on Inflammatory Cytokines Induced by Lipopolysaccharide in Myoblast Cells Under <i>In vitro</i> Environment

Shang, Ke,Amna, Touseef,Amina, Musarat,Al-Musayeib, Nawal M.,Al-Deyab, Salem S.,Hwang, Inho Medknow PublicationsMedia Pvt Ltd 2017 Pharmacognosy magazine Vol.13 No.49

<P><B>Background:</B></P><P>ellular damage initiated by reactive oxygen species (ROS) is the main cause of numerous severe diseases and therefore for this reason, the natural antioxidants have note worthy significance in human health. Capsaicin possesses noteworthy analgesic and anti-inflammatory properties. It also possesses healing effects for treatment of arthritis, diabetic neuropathy, gastric lesions, and cardiac excitability that is why it is incorporated in creams and gels.</P><P><B>Objective:</B></P><P>The present study was carried out to estimate the <I>in vitro</I> antioxidant and ROS scavenging activities of capsaicin against muscle precursor cells. Till date, no investigation has been carried out to study the effect of capsaicin on myoblasts.</P><P><B>Materials and methods:</B></P><P>Herein, the cytotoxicity was induced by endotoxin lipopolysaccharide (LPS) to analyze the effect of capsaicin on LPS induced inflammation and apoptosis on muscle cells. To find out the toxicity of endotoxin, myoblasts were exposed to different concentrations of LPS, viability and morphology was checkedby the means of CCK-8 test and microscopy, respectively. Apoptotic cell death was examined by fluorescence staining. Additionally, LPS-induced apoptosis was determined by mRNAexpression of calpain, caspase-3 and tumor necrosisfactor alpha (TNF-α), and were quantified by qRT-PCR.</P><P><B>Results:</B></P><P>The outcome of the presentstudy demonstrated that LPS stimulation generatestoxicity in dose-dependent manner. Pre-treatmentof myoblasts with capsaicin can considerably alleviate LPS-induced inflammation.</P><P><B>Conclusion:</B></P><P>In conclusion, this study indicates that dietetic supplementation of capsicum may help to alleviate/reduce the inflammatory effects and is therefore potent source of natural antioxidant agent which can be utilized to control muscle related diseases, such as myotube atrophy.</P><P><B>SUMMARY</B></P><P><P>In the present study cytotoxicity was induced by LPS to analyze the effect of capsaicin on LPS induced inflammation and apoptosis on muscle cells.</P><P>The results of this investigation demonstrated that LPS stimulation generates toxicity in dose dependent manner. Pre-treatment of myoblasts with capsaicin can considerably reduce LPS induced inflammation.</P><P>It has been concluded on the basis of results that the dietetic supplementation of capsicum may help to minimize inflammatory effects and are potent sources of natural antioxidants which can be utilized to control muscle related diseases such as atrophy.</P></P> >[FIG OMISSION]</BR><P><B>Abbreviation used:</B> AMP: Adenosine monophosphate, AO/EB: Acridine orange / Ethidium bromide, ATL: T-cell leukemi, CAP: Capsaicin, CCK-8: Cell counting Kit-8, CLSM: Laser Scanning Microscopy, DCF-DA: 2’, 7’-dichlorofluorescein diacetate, DMEM: Dulbecco’s modified Eagle’s medium, DPPH: α, α-diphenyl-β-picrylhydrazyl, FBS: Fetal bovine serum, KA: Kainic acid, LPS: Lipopolysaccharide, MDA: Malondialdehyde, NF-κB: Nuclear factor kgene binding, PBS: Phosphate buffer saline, pNA: p-nitroanilide, RNW: RNase free water, ROS: Reactive oxygen species, TNF-α: Tumor necrosis factor alpha, TRPV1: Transient receptor potential vanilloid 1</P>

Monodispersed 3D MnWO4-TiO2 composite nanoflowers photocatalysts for environmental remediation

M. Shamshi Hassan,Touseef Amna,Salem S. Al-Deyab,김현철,길명섭 한국물리학회 2015 Current Applied Physics Vol.15 No.6

Pollutants from textile industries into water-bodies have caused huge environmental hazards. The semiconductor mediated photocatalytic purification of polluted water is a promising environmental remediation technology. In the present study MnWO4-TiO2 composite nanoflowers endowed with efficient photocatalytic activity have been successfully synthesized by facile hydrothermal approach. XRD, SEM, TEM, EDX spectroscopy and UV-DRS were used to characterize the as-synthesized samples. The average size of composite nanoflower is ~2 μmwhile the nanorods constructing the nanoflowers had the average diameters of 90 nm. The photocatalytic activity of the MnWO4-TiO2 nanoflowers for the degradation of methyl orange (MO) in visible light was much higher than of pristine TiO2 nanorods and MnWO4 nanoflowers respectively. The superior photocatalytic activity could be attributed to the formation of a MnWO4-TiO2 heterojunction in the MnWO4-TiO2 nanoflowers.

One-step synthesis of electrically conductive polyaniline nanostructures by oxidative polymerization method

Dhanusuraman Ragupathy,Palanisamy Gomathi,이수출,Salem S. Al-Deyab,이상학,김한도 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4

A simple, rapid and efficient route to prepare polyaniline nanostructures (PANI-NS) by ‘‘one-step chemical oxidative polymerization’’ method is described. This method possesses advantages in terms of good yield, short reaction time, neat conditions and cost-effectiveness. The morphology of the PANI-NS was investigated by field-emission scanning electron microscopy (FE-SEM). The average diameter of single PANI-NS was calculated to be 376 nm. Results from UV–visible spectroscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis and cyclic voltammetry (CV) reveals the existence of PANI units present in the polymeric nanostructures. Also, electrical conducting property of the PANI-NS was analyzed using four-probe measurement method.

Impact on gene expression in response to silver-decorated titania nanomatrix using an in vitro satellite cell culture model

Amna, Touseef,Hassan, M. Shamshi,Al-Deyab, Salem S.,Khil, Myung-Seob,Hwang, Inho Springer-Verlag 2016 Polymer bulletin Vol.73 No.7

<P>Herein we report the synthesis of novel silver-ornamented titanium dioxide crossbreed nanofibers via electrospinning with improved cellular response for possible muscle tissue engineering applications. Titanium isopropoxide and silver nitrate served as precursors to get high aspect ratio silver-decorated titania nanofibers. For a model application in muscle tissue engineering, muscle satellite cells of local Korean Hanwoo were cultured on synthesized hybrid nanofibers. The standard microarray technique has been employed to screen the potential effects of synthesized nanofibers on genome after the culture of cells on hybrid nanofibers. EDX, EPMA, FT-IR, XRD and TEM analysis confirmed purity, distribution, interaction and crystalline nature of this material while the diameter of these nanofibers estimated from FE-SEM and TEM were between 200 and 300 nm. Cell counting with Kit-8 assay at regular time intervals and phase contrast microscopy data revealed that satellite cells proliferated well on nanofibers and cellular attachments were visible by SEM. Microarray analyses of gene expression response indicated clear changes in gene expression in a range of proliferation and apoptosis. Our results open-up new ways to develop and analyze gene expression biomarkers for satellite cell proliferation and finally muscle tissue engineering.</P>

Self-Assembly Growth of Electrically Conductive Chitosan Nanofibrous Scaffold

Dhanusuraman Ragupathy,김한도,Palanisamy Gomathi,Loganathan Kumaresan,이수출,Salem S. Al-Deyab,이상학 한국고분자학회 2012 Macromolecular Research Vol.20 No.10

We report on the synthesis of an electrically conductive chitosan nanofibrous scaffold (NFS) by a “simple template free self-assembly method’’ from biopolymer chitosan in the presence of inorganic acids as dopants and ammonium persulphate (APS) as an initiator. The morphology of the chitosan nanofibers scaffold (CS-NFS) was examined by field emission scanning electron microscopy (FE-SEM). Physiochemical characterizations of the CSNFS were analyzed by Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis. It is found that the morphology and structure of the CS-NFS strongly depend on the kinds of dopant used. Cyclic voltammogram measurement reveals that CS nanofibers have high electrocatalytic activity compared to chitosan powder. The CS nanofibers are expected to be useful in electrical, optical, and electrochemical devices.

Enhanced Photoelectrochemical Solar Water Splitting Using a Platinum-Decorated CIGS/CdS/ZnO Photocathode.

Mali, Mukund G,Yoon, Hyun,Joshi, Bhavana N,Park, Hyunwoong,Al-Deyab, Salem S,Lim, Dong Chan,Ahn, SeJin,Nervi, Carlo,Yoon, Sam S American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.38

<P>A Cu(InGa)Se2 film was modified with CdS/ZnO for application to solar water splitting. Platinum was electrodeposited on the ZnO layer as a hydrogen evolution catalyst. The effects of the electroplating time and acidity level of the electrolyte on the photocurrent density were studied. The highest photocurrent density of -32.5 mA/cm(2) under 1.5 AM illumination was achieved with an electroplating time of 30 min at a pH of 9. This photocurrent density is higher than those reported in previous studies. The markedly high performance of the CIGS/CdS/ZnO photocathode was rationalized in terms of its type II cascade structure that facilitated efficient charge separation at the interface junction.</P>