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Etiopathogenic role of ERK5 signaling in sarcoma: prognostic and therapeutic implications
Sánchez-Fdez Adrián,Matilla-Almazán Sofía,Del Carmen Sofía,Abad Mar,Arconada-Luque Elena,Jiménez-Suárez Jaime,Chinchilla-Tábora Luis Miguel,Ruíz-Hidalgo Mª José,Sánchez-Prieto Ricardo,Pandiella Atanas 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Sarcomas constitute a heterogeneous group of rare and difficult-to-treat tumors that can affect people of all ages, representing one of the most common forms of cancer in childhood and adolescence. Little is known about the molecular entities involved in sarcomagenesis. Therefore, the identification of processes that lead to the development of the disease may uncover novel therapeutic opportunities. Here, we show that the MEK5/ERK5 signaling pathway plays a critical role in the pathogenesis of sarcomas. By developing a mouse model engineered to express a constitutively active form of MEK5, we demonstrate that the exclusive activation of the MEK5/ERK5 pathway can promote sarcomagenesis. Histopathological analyses identified these tumors as undifferentiated pleomorphic sarcomas. Bioinformatic studies revealed that sarcomas are the tumors in which ERK5 is most frequently amplified and overexpressed. Moreover, analysis of the impact of ERK5 protein expression on overall survival in patients diagnosed with different sarcoma types in our local hospital showed a 5-fold decrease in median survival in patients with elevated ERK5 expression compared with those with low expression. Pharmacological and genetic studies revealed that targeting the MEK5/ERK5 pathway drastically affects the proliferation of human sarcoma cells and tumor growth. Interestingly, sarcoma cells with knockout of ERK5 or MEK5 were unable to form tumors when engrafted into mice. Taken together, our results reveal a role of the MEK5/ERK5 pathway in sarcomagenesis and open a new scenario to be considered in the treatment of patients with sarcoma in which the ERK5 pathway is pathophysiologically involved.
SiC Substrate Effects on Electron Transport in the Epitaxial Graphene Layer
Engin Arslan,Semih Çakmakyapan,Özgür Kazar,Serkan Bütün,Sefer Bora Li esivdin,Neval A. Cinel,Gülay Ertas,Sükrü Ardal,Engin T ras,Jawad-ul-Hassan,E. Janzén,Ekmel Özbay 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.2
Hall effect measurements on epitaxial graphene (EG) on SiC substrate have been carried out as a function of temperature. The mobility and concentration of electrons within the two-dimensional electron gas (2DEG) at the EG layers and within the underlying SiC substrate are readily separated and characterized by the simple parallel conduction extraction method (SPCEM). Two electron carriers are identified in the EG/SiC sample: one highmobility carrier (3493 cm2/Vs at 300 K) and one low-mobility carrier (1115 cm2/Vs at 300 K). The high mobility carrier can be assigned to the graphene layers. The second carrier has been assigned to the SiC substrate.
Interaction of surface water waves with small bottom undulation on a sea-bed
S. C. Martha,S. N. Bora,A. Chakrabarti 한국전산응용수학회 2009 Journal of applied mathematics & informatics Vol.27 No.5
The problem of interaction of surface water waves by small undulation at the bottom of a laterally unbounded sea is treated on the basis of linear water wave theory for both normal and oblique incidences. Perturbation analysis is employed to obtain the first order corrections to the reflection and transmission coefficients in terms of integrals involving the shape function c(x) representing the bottom undulation. Fourier transform method and residue theorem are applied to obtain these coefficients. As an example, a patch of sinusoidal ripples is considered in both the cases as the shape function. The principal conclusion is that the reflection coefficient is oscillatory in the ratio of twice the surfacewave number to the wave number of the ripples. In particular, there is a Bragg resonance between the surface waves and the ripples, which is associated with high reflection of incident wave energy. The theoretical observations are validated computationally The problem of interaction of surface water waves by small undulation at the bottom of a laterally unbounded sea is treated on the basis of linear water wave theory for both normal and oblique incidences. Perturbation analysis is employed to obtain the first order corrections to the reflection and transmission coefficients in terms of integrals involving the shape function c(x) representing the bottom undulation. Fourier transform method and residue theorem are applied to obtain these coefficients. As an example, a patch of sinusoidal ripples is considered in both the cases as the shape function. The principal conclusion is that the reflection coefficient is oscillatory in the ratio of twice the surfacewave number to the wave number of the ripples. In particular, there is a Bragg resonance between the surface waves and the ripples, which is associated with high reflection of incident wave energy. The theoretical observations are validated computationally
Débora M.Borsato,Arthur S. Prudente,Patrícia M. Döll-Boscardin,Aurélio V. Borsato,Cynthia F.P. Luz,Beatriz H.L.N.S. Maia,Daniela A. Cabrini,Michel F. Otuki,Marilis D. Miguel,Paulo V. Farago,Obdulio G. 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.7
Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the highperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 – 5%. This extract decreased the myeloperoxidase activity in 75 – 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 – 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.
Review : Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives
( Mohammed N. Baeshen ),( Ahmed M. Al Hejin ),( Roop S. Bora ),( Mohamed M. M. Ahmed ),( Hassan A. I. Ramadan ),( Kulvinder S. Saini ),( Nabih A. Baeshen ),( Elrashdy M. Redwan ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7
Escherichia coli is the most preferred microorganism to express heterologous proteins for therapeutic use, as around 30% of the approved therapeutic proteins are currently being produced using it as a host. Owing to its rapid growth, high yield of the product, costeffectiveness, and easy scale-up process, E. coli is an expression host of choice in the biotechnology industry for large-scale production of proteins, particularly non-glycosylatedproteins, for therapeutic use. The availability of various E. coli expression vectors and strains, relatively easy protein folding mechanisms, and bioprocess technologies, makes it very attractive for industrial applications. However, the codon usage in E. coli and the absence ofpost-translational modifications, such as glycosylation, phosphorylation, and proteolytic processing, limit its use for the production of slightly complex recombinant biopharmaceuticals. Several new technological advancements in the E. coli expression system to meet the biotechnology industry requirements have been made, such as novel engineered strains,genetically modifying E. coli to possess capability to glycosylate heterologous proteins an express complex proteins, including full-length glycosylated antibodies. This review summarizes the recent advancements that may further expand the use of the E. coli expression system to produce more complex and also glycosylated proteins for therapeutic use in the future.
Artur N. Carreiro,João A. R. A. Diniz,Joyce G. Souza,Débora V. F. Araújo,Rômulo F. F. Dias,Liliane M. S. Azerêdo,Ediane F. Rocha,Ana Y. F. La Salles,Carlos E. Peña-Alfaro,Maria A. M. Carvalho,Maria J. 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.3
The objective of the present study was to monitor and characterize morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by using abdominal ultrasonography. All animals underwent daily vaginal cytological examination to identify the current cycle phase. For each phase of the estrous cycle, ultrasound examinations were carried out to identify and describe the morphology of both ovaries. Topographic parameters in an ultrasound window were established to locate the ovaries. The agouti estrous cycle lasted an average of 29.94 ± 6.77 days. During vaginal cytology examinations, all cell types were identified, and each phase of the estrous cycle was established by cell counts. No significant alterations were observed in the assessed ovarian morphometry measurements. In 75% of the animals examined, ovarian follicle presence was observed in the proestrus phase.
INTERACTION OF SURFACE WATER WAVES WITH SMALL BOTTOM UNDULATION ON A SEA-BED
Martha, S.C.,Bora, S.N.,Chakrabarti, A. The Korean Society for Computational and Applied M 2009 Journal of applied mathematics & informatics Vol.27 No.5
The problem of interaction of surface water waves by small undulation at the bottom of a laterally unbounded sea is treated on the basis of linear water wave theory for both normal and oblique incidences. Perturbation analysis is employed to obtain the first order corrections to the reflection and transmission coefficients in terms of integrals involving the shape function c(x) representing the bottom undulation. Fourier transform method and residue theorem are applied to obtain these coefficients. As an example, a patch of sinusoidal ripples is considered in both the cases as the shape function. The principal conclusion is that the reflection coefficient is oscillatory in the ratio of twice the surface wave number to the wave number of the ripples. In particular, there is a Bragg resonance between the surface waves and the ripples, which is associated with high reflection of incident wave energy. The theoretical observations are validated computationally.