자외선 B 조사로 유도되는 염증 반응과 지질과산화에 대한 Egb 761의 억제 효과

최욱희,안령미 생활과학연구소 2005 생활과학연구 Vol.10 No.-

Reactive oxygen species are involved in inflammatory reaction & lipid peroxidation of skin induced by ultraviolet radiation. The aim of this study was to investigate a protective effect of topical EGb 761 on ultraviolet B induced inflammation and lipid peroxidation in ICR mice. Lipid peroxidation in skin was assessed by thiobarbituric acid reactive substances(TBARS) levels, while skin-fold thickness was used as the measure of UVB-induced inflammation. EGb 761 was topically applied to the skin for 7 days before a single dose of 2KJ/㎡ UVB irradiation. In irradiated skin, maximal skin swelling occurred at 24h, lipid peroxides were increased at 3h after irradiation. Topical application of 1% EGb 761 reduced edema response and lipid peroxide levels. In conclusion, we suggest that topical application of EGb 761 before UVB irradiation acts as a photoprotective agent.

서울시 일부 여성 노인의 뇨중 카드뮴함량에 관한 연구

이세나,안령미 동덕여자대학교 2002 생활과학연구 Vol.7 No.-

We collected urine samples from 118 old female on Sungbukgu area in Seoul city. They were not occupationally exposed to cadmium and female aged over 60 years. The summarized results were as follows: The average urine cadmium level of subjects was 3.54㎍/g Cr and urine calcium level of subject was 0.23㎎/g Cr Urine calcium level was significantly correlated eating fish meal (p<0.05) but not urine cadmium was not correlated it. Urine cadmium was significantly correlated with living in farm village rather than fish village (P<0.05) Urine calcium was positively correlated with pregnancy and labor. (p<0.05)

성북구 거주 거동 불편 여성노인의 생활만족도에 관한 연구

윤종희,김덕성,안령미 동덕여자대학교 한국여성연구소 2002 젠더연구 Vol.- No.6

The purpose of the study was to investigate relationships among life satisfaction of the elderly women in poor-health conditions, with their self-esteem, health and financial status, family relations, and social participation. A structured questionnaire was used to interview a sample of 168 elderly women who were randomly selected from Sungbuk-ku, Seoul. The statistical methods used were percentiles, means, standard deviations, Cronbach`s α, Pearson`s correlations and a multiple regression. The regression analysis showed that the most powerful predictor of satisfaction of the poor-health elderly women was their self esteem, followed by relationships with their adult children, perceived financial status, age, and the amount of allowances. These variables accounted for 41.7% of life satisfaction of the sample. Implications for the policy-makers and practitioners were further discussed.

Effects of Natural Extracts on COX-1 and COX-2 mRNA Expression on UVB-induced Skin Inflammation in C57BL/6 Mouse

Ahn, Ryoung-Me Korean Society of Environmental Health 2006 한국환경보건학회지 Vol.32 No.6

Exposure to ultraviolet B(UVB) radiation causes skin inflammation such as pigmentation and the induction of cyclooxygenase-2(COX-2) gene expression. In this study, we investigated the effect of natural extracts from Tea, EGb 761 and Korean red ginseng(KRG), on the pigmentation and expression of COX-1 and COX-2 mRNA in UVB-irradiated C57BL/6 mice. Before UVB irradiation, the skin color was significantly showed the lightening effect by topical application of natural compounds (p<.05). In the case of UVB irradiated mice, we observed a decrease in pigmentation by compounds (p<.05). In irradiated skin, COX-1 mRNA expression is not changed following UVB irradiation, but COX-2 gene increases. Also, natural compounds lowered mRNA levels of COX-2. Therefore, these results suggest that COX-2 mRNA increases by UVB irradiation. Also, Tea, EGb 761 and KRG as a topical application may inhibit skin pigmentation and modulate COX-2 mRNA level.

카드륨 독성에 대한 부추 (Allium Odorum L.) 의 방어효과

안령미(Ryoung Me Ahn),김완태(Wan Tai Kim),이희성(Hi Sung Lee) 한국환경보건학회 1991 한국환경보건학회지 Vol.17 No.2

This study aimed to find out the effect of freeze -drying leek against cadmiurn poisoning on the cholesterol and enzyme activities in serum and superoxide radical, SOD and catalase in liver and kidney of the rnale rats during the administrered period. ln this experiment, male rats of SPragμe-Dawley strain were used. The rats were divided into 3 groups which were fed different1y either for 5 weeks or for 10 weeks : basal diet, basal diet and cadmium in water and 3 % leek added diet and cadmium in water. Cadmium was administered ad libltum 100 ppm CdCh in water. The followings are the results of this experiment. l. Leek reduced the cholesterol and the activities of GPT increase resulted from cadmium treatment. 2. Leek reduced the rate of cadmium in liver and kidney. 3. Leek reduced the activities of SOR and catalase in liver and kidney, while it enhanced the activities of SOD. 4. Leek reduced the necrosis and swelling in liver and kidney casused by cadmium treatment. This experiment showed that leek -addition group had protective effect agair:st cadmium pois oning and increased ALPase activities in serum. Leek alleviated GPT activities m serum and cad•rnium concentration, necrosis, and swelling in liver and kidney, Therefore, this experiment con c1uded that leek has defenslve power against cadmium poisoning.

보문 : 자외선에 의한 비타민 D3 합성과 직물 ( 제 1 보 )

안령미(Ryoung Me Ahn),송명견(Myung Kyun Song) 한국의류학회 1997 한국의류학회지 Vol.21 No.5

자외선에 의한 비타민 D_3 합성과 직물 (제2보) : 동물 실험을 통하여 The animal study

안령미,김정현,송명건 한국의류학회 1998 한국의류학회지 Vol.22 No.5

Because there is a great. concern today about the damaging effect of chronic exposure to sunlight the use of sunscreen providing the photoprotection effect against ultraviolet (UV) was widely increased. As a result of common use of level of photosynthetic Vit. D_3 in human skin decreased these days. In our experiment the animals covered with fabrics with 50% (fabric B) and 100% (fabric A) protection rate against ultraviolet B (UVB) were used to measure serum 25(OH)D_3, ALP, total clacium and phosphorus. Vitamin D deficiency diet group had no effect on concentration of serum phosphorus. But the concentrations of serum 25(OH)D_3 and total calcium were more decreased in vitamin D deficiency diet rats than in normal diet rats. Alkaline phosphatase activity in sunlight irradiated groups covered with 50% (fabric B) and 100% (fabric A) UVB protection fabrics was more significantly decreased than vitamin D deficiency diet group. In conclusion, sunlight irradiateted groups were compared to effective to protect born disease due to the Vit. D deficeincy group.

비타민 D 결핍식이 쥐에서의 태양광선 조사에 의한 malondialdehyde 생성측정과 alkaline phosphatase 변화에 관한 연구

안령미,김정현,장동덕 대한보건협회 1998 대한보건연구 Vol.24 No.1

비타민 D는 칼슘과 인의 세포외액농도를 정상범위로 유지하는 비타민이다. 또한 골의 피괴와 형성과도 관계가 있다. 비타민 D는 두 가지 공급원에 의해서 공급되어 지는데, 첫 번째는 식이에 의한 섭취, 두 번째는 피부에서의 합성이다. 본 연구에서는 일반식이와 비타민 D 결핍식이 쥐에 태양광선을 조사하였을 경우 혈청 25(OH)D_3, 칼슘, 인, alkaline phosphatase(ALP)의 변화를 연구하고자 하였다. 또한 실험동물에 태양광선의 조사가 과산화지질의 지표로 사용되는 malondialdehyde 농도에 미치는 영향을 관찰하였다. 어린 암컷의 SD rats를 1)일반식이, 2) 일반식이+태양광선, 3) 비타민 D 결핍식이, 4) 비타민 D 결핍식이+태양광선의 4군으로 분류하였다. 비타민 D결핍식이는 혈청 칼슘과 인의 농도에 영향을 미치지 않았다. 하지만 혈청 25(OH)D_3 농도의 경우 일반식이군에 비하여 비타민 D 결핍식이 군에서 유의하게 감소하였으며, 비타민 D 결핍식이군에 태양광선을 조사하였을 때 증가하였다. ALP 활성의 경우 일반식이나 비타민 D 결핍식이군에 비해 태양광선을 조사한 군들에서 감소를 보였다. 또한 일반식이보다 비타민 D 결핍식이군에서 ALP의 활성이 낮았다. Malondialdehyde의 생성은 탸양광선 비조사군에 비하여 조사군에서 감소하였다. 결론적으로 실험동물에 태양광선을 조사한 경우 칼슘, 인, 25(OH)D_3 의 혈청농도에는 변화를 주지 않았지만 골질환의 인지지표인 ALP에 있어서는 유의한 차이를 나타냈다. 즉 비타민 D 결핍식이를 공급한 쥐에 있어 태양광선을·조사하였을 경우에는 통계적으로 유의하게 ALP가 감소하였다. 이는 비타민 D의 결핍으로 인한 ALP의 상승을 태양광선의 조사에 의해 완화된다는 것을 나타낸다. Vitamin D is a unique vitamin and hormone which maintains extracellular fluid concentrations of calcium and phosphorus within the normal range. Additionally, it is related with bone destruction and bone formation. The vitamin D is supplied with two sources 1) ingested in the diet, 2) produced in the skin. We now have examined the effect of sunlight exposure on serum 25(OH)D_3, calcium, phosphorus and alkaline phosphatase in rat with normal or vitamin D deficiency diet. Also we have observed the malondialdehyde concentration as an index of lipid peroxidation when rats was irradiated with sunlight. Weanling female Sprague Dawely rats were randomly divided into 4 groups : 1) normal diet, 2) normal diet with sunlight exposure, 3) vitamin D deficiency diet, 4) vitamin D deficiency diet with sunlight exposure. Vitamin D deficiency diet had no effect on concentration of serum calcium and phosphorus. But the concentrations of serum 25(OH)D_3 was decreased in rat with vitamin D deficiency diet compared to rats with normal diet. Serum 25(OH)D_3 concentration was increased by sunlight exposure in vitamin D deficiency diet group. In alkaline phosphatase activity, it was decreased in sunlight irradiated groups compared to normal and vitamin D deficiency diet groups. And the activity of alkaline phosphatase was lowered in vitamin D deficiency diet group than normal diet one. Sunlight decreased the synthesis of malondialdehyde in irradiated group compared to non-irradiated group. These results suggest that the sunlight irradiation had lowered the ALP concentration in serum. And it implied that the sunlight exposure may produce an beneficial effect on the bone.

직물의 자외선차단과 세포에 미치는 방호효과

안령미,이수진,송명견 한국의류학회 1997 한국의류학회지 Vol.21 No.4

The purpose of the study was to investigate a transmittance rate of UVB (Ultraviolet B) through summer fabrics and a protection rate of summer fabric from UVB. The subjects were randomly selected 159 fabrics from Korean common summer fabrics. The protection rates of 159 fabrics from UVB were measured by UVB lamp and UVB sensor, and 14 fabrics among these fabrics were selected for an assay of MTT(3-[4, 5-dimethylthiazol-2-yl] -2, 5 -diphenyltetrazolium). The protection rate of fabrics from cell toxicity of UVB was measured by investigating the difference of the amount of cell toxic substance on between fabrics covered with and without HeLa cell. The average protection rate of 159 fabrics from UVB was 95.08%. As result findings, three negative correlations were found between: 1) the transmittance rate of UVB and the amount of MTT on fabrics (y=0.0373+0.00518 x, r=-0.9323, p<0.001); 2) the air permeability of fabrics and the amount of MTT (r= -0.79, p<0.01); 3) the air permeability of fabrics and the protection rate of fabrics from UVB (r=0.89, p<0.01). However, there was no effect of thickness of fabrics on the protection rate from UVB and the amount of MTT.

비타민 D 결핍식이 쥐에서의 태양광성 조사에 의한 malondialdehyde 생성측정과 alkaline phosphatase 변화에 관한 연구

안령미,김정현,장동덕 대한보건협회 1998 대한보건연구 Vol.24 No.1

비타민 D는 칼슘과 인의 세포외액농도를 정상범위로 유지하는 비타민이다. 또한 골의 파괴와 형성과도 관계가 있다. 비타민 D는 두 가지 공급원에 의해서 공급되어 지는데, 첫 번째는 식이에 의한 섭취, 두 번째는 피부에서의 합성이다. 본 연구에서는 일반식이와 비타민 D 결핍식이 쥐에 태양광선을 조사하였을 경우 혈청 25(OH)D_3, 칼슘, 인, alkaline phosphatase(ALP)의 변화를 연구하고자 하였다. 또한 실험동물에 태양광선의 조사가 과산화지질의 지표로 사용되는 malondialdehyde 농도에 미치는 영향을 관찰하였다. 어린 암컷의 SD rats를 1)일반식이, 2) 일반식이+태양광선, 3) 비타민 D 결핍식이, 4) 비타민 D 결핍식이+태양광선의 4군으로 분류하였다. 비타민 D 결핍식이는 혈청 칼슘과 인의 농도에 영향을 미치지 않았다. 하지만 혈청 25(OH)D_3 농도의 경우 일반식이군에 비하여 비타민 D 결핍식이 군에서 유의하게 감소하였으며, 비타민 D 결핍식이군에 태양광선을 조사하였을 때 증가하였다. ALP 활성의 경우 일반식이나 비타민 D 결핍식이군에 비해 태양광선을 조사한 군들에서 감소를 보였다. 또한 일반식이보다 비타민 D 결핍식이군에서 ALP의 활성이 낮았다. Malondialdehyde의 생성은 태양광선 비조사군에 비하여 조사군에서 감소하였다. 결론적으로 실험동물에 태양광선을 조사한 경우 칼슘, 인, 25(OH)D_3의 혈청농도에는 변화를 주지 않았지만 골질환의 인지지표인 ALP에 있어서는 유의한 차이를 나타냈다. 즉 비타민 D 결핍식이를 공급한 쥐에 있어 태양광선을 조사하였을 경우에는 통계적으로 유의하게 ALP가 감소하였다. 이는 비타민 D의 결핍으로 인한 ALP의 상승을 태양광선의 조사에 의해 완화된다는 것을 나타낸다. Vitamin D is a unique vitamin and hormone which maintains extracellular fluid concentrations of calcium and phosphorus within the normal range. Additionally, it is related with bone destruction and bone formation. The vitamin D is supplied with two sources 1) ingested in the diet, 2) produced in the skin. We now have examined the effect of sunlight exposure on serum 25(OH)D_3, calcium, phosphorus and alkaline phosphatase in rat with normal or vitamin D deficiency diet. Also we have observed the malondialdehyde concentration as an index of lipid peroxidation when rats was irradiated with sunlight. Weanling female Sprague Dawely rats were randomly divided into 4 groups: 1) normal diet, 2) normal diet with sunlight exposure, 3) vitamin D deficiency diet, 4) vitamin D deficiency diet with sunlight exposure. Vitamin D deficiency diet had no effect on concentration of serum calcium and phosphorus. But the concentrations of serum 25(OH)D_3 was decreased in rat with vitamin D deficiency diet compared to rats with normal diet. Serum 25(OH)D_3 concentration was increased by sunlight exposure in vitamin D deficiency diet group. In alkaline phosphatase activity, it was decreased in sunlight irradiated groups compared to normal and vitamin D deficiency diet groups. And the activity of alkaline phosphatase was lowered in vitamin D deficiency diet group than normal diet one. Sunlight decreased the synthesis of malondialdehyde in irradiated group compared to non-irradiated group. These results suggest that the sunlight irradiation had lowered the ALP concentration in serum. And it implied that the sunlight exposure may produce an beneficial effect on the bone.