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Grease matrix as a versatile carrier of proteins for serial crystallography
Sugahara, Michihiro,Mizohata, Eiichi,Nango, Eriko,Suzuki, Mamoru,Tanaka, Tomoyuki,Masuda, Tetsuya,Tanaka, Rie,Shimamura, Tatsuro,Tanaka, Yoshiki,Suno, Chiyo,Ihara, Kentaro,Pan, Dongqing,Kakinouchi, Ke Nature Publishing Group 2015 NATURE METHODS Vol. No.
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid–binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
Tamaki Tanaka,Kazuhiro Takehara,Natsumi Yamashita,Mika Okazawa-Sakai,Kazuya Kuraoka,Norihiro Teramoto,Kenichi Taguchi,Katsushige Yamashiro,Hidenori Kato,Tomoya Mizunoe,Rie Suzuki,Dan Yamamoto,Arisa Ue 대한부인종양학회 2022 Journal of Gynecologic Oncology Vol.33 No.5
Objective: To clarify the frequency of deficient mismatch repair (dMMR) in Japanese ovarian cancer patients, we examined microsatellite instability (MSI) status and immunohistochemistry (IHC) subtypes, including endometrioid carcinoma (EMC), clear cell carcinoma (CCC), or a mixture of both (Mix). Methods: We registered 390 patients who were diagnosed with EMC/CCC/Mix between 2006 and 2015 and treated at seven participating facilities. For 339 patients confirmed eligible by the Central Pathological Review Board, MSI, IHC, and MutL homolog 1 methylation analyses were conducted. The tissues of patients with Lynch syndrome (LS)-related cancer histories, such as colorectal and endometrial cancer, were also investigated. Results: MSI-high (MSI-H) status was observed in 2/217 CCC (0.9%), 10/115 EMC (8.7%), and 1/4 Mix (25%). Additionally, loss of MMR protein expression (LoE-MMR) was observed in 5/219 (2.3%), 16/115 (14.0%), and 1/4 (25%) patients with CCC, EMC, and Mix, respectively. Both MSI-H and LoE-MMR were found significantly more often in EMC (p<0.001). The median (range) ages of patients with MMR expression and LoE-MMR were 54 (30–90) and 46 (22–76) (p=0.002), respectively. In the multivariate analysis, advanced stage and histological type were identified as prognostic factors. Conclusion: The dMMR rate for EMC/CCC was similar to that reported in Western countries. In Japan, it is assumed that the dMMR frequency is higher because of the increased proportion of CCC.
Dynamic Chest X-Ray Using a Flat-Panel Detector System: Technique and Applications
Hata Akinori,Yamada Yoshitake,Tanaka Rie,Nishino Mizuki,Hida Tomoyuki,Hino Takuya,Ueyama Masako,Yanagawa Masahiro,Kamitani Takeshi,Kurosaki Atsuko,Sanada Shigeru,Jinzaki Masahiro,Ishigami Kousei,Tomiy 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.4
Dynamic X-ray (DXR) is a functional imaging technique that uses sequential images obtained by a flat-panel detector (FPD). This article aims to describe the mechanism of DXR and the analysis methods used as well as review the clinical evidence for its use. DXR analyzes dynamic changes on the basis of X-ray translucency and can be used for analysis of diaphragmatic kinetics, ventilation, and lung perfusion. It offers many advantages such as a high temporal resolution and flexibility in body positioning. Many clinical studies have reported the feasibility of DXR and its characteristic findings in pulmonary diseases. DXR may serve as an alternative to pulmonary function tests in patients requiring contact inhibition, including patients with suspected or confirmed coronavirus disease 2019 or other infectious diseases. Thus, DXR has a great potential to play an important role in the clinical setting. Further investigations are needed to utilize DXR more effectively and to establish it as a valuable diagnostic tool.
Napoleon Enteria,Hiroshi Yoshino,Akashi Mochida,Rie Takaki,Akira Satake,Seizo Baba,Yasumitsu Tanaka 대한설비공학회 2015 International Journal of Air-Conditioning and Refr Vol.23 No.4
The desiccant-based air-conditioning system consists of many heat and mass transfer components operating together with air fans. The performance test using the multiple tracer gas dilution method is important for knowing the internal and external flow rates, including the air leakages. The results of the test show the actual internal air flow rates and the sources of air leakage, and also show the interaction of air in the air-conditioned lecture room and in the mechanical room. The performance test using the multiple tracer gas dilution method in air flow and air leakages is important for a detailed evaluation of any air-conditioning system such as this desiccant-based one.
A Survey Among Wheelchair Users Who Participated in Banie Free 2010 Trade Show
Toru Furui,Haruka Onishi,Masahiro Kagatanj,Sayaka Sako,Kensuke Hiramoto,Rie Yonezawa,Takashi Handa,Minoru Tanaka 한국재활복지공학회 2010 한국재활복지공학회 학술대회논문집 Vol.2010 No.11
Objectives of this study is to explore factors impacting their pain and discomfort, and to advocate seating ergonomics in public. We set up a seating clinic booth and conducted survey among wheelchair users at Barrie Free 2010 Trade Show held at Intec Osaka from April 15th to April 17<SUP>th</SUP> 2010.Weanalyzedtheseated posture using software "Rysis", which designed based on ISO 16840-1. Results showed pelvis and trunk asymmetry, joystick controller side, and neck pain made impact on their seated posture. With this clinical consultation process of this work, users can satisfy their interest in objective findings of their seated posture, and experience the importance of ergonomic seating.
A three-dimensional movie of structural changes in bacteriorhodopsin
Nango, Eriko,Royant, Antoine,Kubo, Minoru,Nakane, Takanori,Wickstrand, Cecilia,Kimura, Tetsunari,Tanaka, Tomoyuki,Tono, Kensuke,Song, Changyong,Tanaka, Rie,Arima, Toshi,Yamashita, Ayumi,Kobayashi, Jun American Association for the Advancement of Scienc 2016 Science Vol.354 No.6319
<P>Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.</P>