Effect of First Line Gastric Cancer Chemotherapy Regime on the AGS Cell Line - MTT Assay Results

Alizadeh-Navaei, Reza,Rafiei, Alireza,Abedian-Kenari, Saeid,Asgarian-Omran, Hossein,Valadan, Reza,Hedayatizadeh-Omran, Akbar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.1

Background: Combination chemotherapy regimes are common treatments for cancer. The aim of this study was to evaluation the effect of individual chemotherapeutic agents in comparison with a first line chemotherapy regime treatment in the AGS gastric cancer cell line by MTT assay. Materials and Methods: In this experimental study, AGS cells were grown in RPMI-1640 supplemented with 10% fetal calf serum and 100 IU/ml penicillin, and $10{\mu}g/ml$ streptomycinin, under a humidified condition at $37^{\circ}C$ with 5% CO2. All cells were washed with PBS and detached with trypsin, centrifuged and 8000 cells re-plated on to 96- well plates. LD50 doses of Epirubicin, Cisplatin and 5-fluorouracil were added to each well in mono or triple therapy. Anti-proliferative activities were determined by MTT assay after 24, 48 or 72 h. Results: Results of MTT assays showed that there were no significant differences among 3 drugs in monotherapy (p=0.088), but there was significant difference between combination therapy with epirubicin (P=0.031) and 5FU (p=0.013) on cell survival at 24 h. After 48 and 72 hours, cell viability showed significant differences between the 3 drugs (p=0.048 and P=0.000 for 48 and 72 h, respectively) and there was significant difference between combination therapy with epirubicin (P=0.035 and P=0.002 for 48 and 72 h, respectively). Conclusions: The results showed no significant differences between these chemotherapy drugs each given alone, but combination therapy with 3 drugs had significant effects on cell viability in comparison with epirubicin alone.

Acupuncture in Preventing Postoperative Nausea and Vomiting: Efficacy of Two Acupuncture Points Versus a Single One

Reza Alizadeh,Sara Esmaeili,Saeed Shoar,Shahram Bagheri-Hariri,Nasrin Shoar 사단법인약침학회 2014 Journal of Acupuncture & Meridian Studies Vol.7 No.2

Despite recent advances in anesthesiology and postoperative care, postoperative nausea and vomiting are common complaints. Although acupuncture techniques have received attention in anesthesiology, the ideal technique and selection of the most appropriate acupuncture points are still under debate. This study compared the efficacy of two simul- taneous acupuncture points with that of a single point in the prevention and treatment of postoperative nausea and vomiting following general anesthesia through a double-blind, randomized, controlled trial involving 227 surgical patients undergoing general anes- thesia who were randomly assigned into two groups. The first group received acupuncture by stimulation only on the PC6 point (single group), and the second group underwent concomitant stimulation of the PC6 and the L14 acupuncture points (combined group) during surgery under general anesthesia. The prevalences of postoperative nausea and vomiting were compared between the two groups. No significant differences were observed between the two groups (p > 0.05). Of 115 patients in the combined group, 80 (69.6%) complained about nausea and vomiting compared with 96 (85.7%) in the single group, a significantly lower proportion (p < 0.05). Our findings favor a combination of PC6 and LI4 stimulation for the treatment of postoperative nausea and vomiting.

Adsorption of methylene blue from an aqueous dyeing solution by use of santa barbara amorphous-15 nanostructure: Kinetic and isotherm studies

Alizadeh, Reza,Zeidi, Amir Techno-Press 2017 Advances in environmental research Vol.6 No.2

Santa Barbara Amorphous-15(SBA-15) nanoparticles were utilized as the inexpensive and effective adsorbents to remove methylene blue dye from the aqueous solution.SBA-15 was created by Zhao et al method. Infrared spectroscopy, X-ray diffraction and scanning electron microscopy (SEM) were used for the evaluated physical properties of SBA-15. The results of diffraction X-ray indicated that was the crystalline structure for it. Also IR spectroscopy indicated was a silica the whole structure of the groups and SEM image verify the structure of relatively identical particles size of SBA-15. Factors affecting adsorption including the amounts of adsorbent, pH and contact time were investigated by a SBA-15 nanomaterial design. The extent of dye removal enhanced with increasing initial dye concentration and pH from 4 to 10. The higher percentage adsorption were obtained under optimum conditions of variables (sorbent dose of 200 mg/liter, initial MB concentration 10 mg/liter, initial pH of 10 and temperature of $25^{\circ}C$). Maximum adsorption happened after the 2 hour and the kinetic processes of the dyes adsorption were described by applying the pseudo-first-order and the pseudo-second-order and the relatively High correlation with the kinetic Ellovich models. It was found that the pseudo-second-order models kinetic equation described the data of dye adsorption with a good correlation (R2>0.999) which indicated chemisorption mechanism. Freundlich and Langmuir adsorption models were investigated in conditions of variables (adsorbent dose 0.01 gr/liter, MB concentration 10, 20, 30 mg/liter, pH of 4, 7, 10, contact time 90 min and temperature of $27^{\circ}C$). The adsorption data were represented by Langmuir isotherm model. These values are higher than the adsorption capacities of some other adsorbents that have recently been published in the literature.

High Frequency of blaOXA-48like Producing Klebsiella pneumoniae Isolated from Nosocomial Infection in Azerbaijan, Iran

Ghotaslou Reza,Salahi Behnaz,Naderi Ghazal,Alizadeh Naser 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.1

Background: Klebsiella pneumoniae is one of the significant agents of hospital-acquired infections. In recent years, carbapenem-resistant K. pneumoniae (CRKP) isolates have been found in numerous epidemics of nosocomial infections. This study aimed to determine carbapenem resistance mechanisms and molecular epidemiological of CRKP infections in Azerbaijan, Iran. Materials and Methods: A total of 50 non-duplicated CRKP from January 2020 to December 2020 were isolated form Sina and Imam Reza Hospitals in Tabriz, Iran. Antimicrobial susceptibility testing was performed by the disk-diffusion method. The carbapenem resistance mechanisms were determined by the phenotypic and PCR procedures. CRKP isolates were typed by the Random Amplified Polymorphic DNA PCR (RAPD-PCR) technique. Results: Amikacin was the most effective antibiotics against CRKP isolates. AmpC overproduction was observed in five CRKP isolates. Efflux pump activity was found in one isolate by the phenotypic method. Carba NP test could find carbapenemases genes in 96% of isolates. The most common carbapenemases gene in CRKP isolates were blaOXA-48-like (76%) followed by blaNDM (50%), blaIMP (22%), blaVIM (10%), and blaKPC (10%). The outer membrane protein genes (OmpK36 and OmpK35) were identified in 76% and 82% of CRKP isolates, respectively. RAPD-PCR analysis yielded 37 distinct RAPD-types. Most blaOXA-48-like positive CRKP isolates were obtained from patients hospitalized in intensive care unit (ICU) wards with urinary tract infections. Conclusion: The blaOXA-48-like is the main carbapenemase among CRKP isolates in this area. Most blaOXA-48-like producer CRKP strains were collected from the ICU ward and urine samples. To control infections due to CRKP, a strict control program in hospital settings is required.

Cholangiocarcinoma: An-eight-year Experience in a Tertiary-Center in Iran

Mohammad-Alizadeh, Amir Houshang,Ghobakhlou, Mehdi,Shalmani, Hamid Mohaghegh,Zali, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Background and Aim: Cholangiocarcinoma (CCA) is an uncommon malignancy of the bile duct, occurring in nearly 2 out of 100,000 people. It is a type of adenocarcinoma that originates in the mucous glands of the epithelium, or surface layers of the bile ducts. The aim of this study was to evaluate the clinical features, diagnostic results and factors associated with survival, morbidity and mortalityof cholangiocarcinoma cases in Iranian patients. Method: In this retrospective study the hospital medical records of 283 patients with a primary or final diagnosis of cholangiocarcinoma who had been admitted to gastroenterology ward of our hospital from 2004 to 2011 were retrospectively reviewed. Results: 283 patients (180 male, 63%, and 103 female, 38.6%) with a mean age of $59.7{\pm}14.4$ years were studied. The most frequent symptoms were painless jaundice (190, 66.9%), abdominal pain (77, 27%), pruritus 133 (46.8%) and weight loss (169, 59.5%). The most frequent associated risk factors and diseases were as follows: gallstones (72, 25.4%), diabetes (70, 24.6%), HBV infection (52 (18.3%), HCV infection 43 (15%), primary sclerosing cholangitis (16, 5.6%) and smoking (120, 42.3%). The most frequent type of cholangiocarcinoma in ERCP and MRCP was hilar. The mean survival time was $7.42{\pm}5.76$ months. Conclusion: The mean survival time in our study was lower than one year. Moreover the most frequent risk factors and associated diseases were smoking, gallstones and diabetes. Painless jaundice, abdominal pain and weight loss were the most clinical features related to cholangiocarcinoma. Additionally survival time did not correlate with risk factors, associated diseases and clinical presentations, but was linked to biliary metallic stenting and surgery.

Diagnosis of Malignant Biliary Strictures: Conventional or Negative Pressure Brush Cytology?

Abbasi, Mohammad Reza,Mirsaeed, Seyedeh Masoumeh Ghazi,Alizadeh, Amir Houshang Mohammad Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.10

Background/Objective: The aim of this study was to perform a comparative evaluation of the yields of conventional brush cytology and brush cytology with negative pressure in the diagnosis of malignant biliary strictures. Methods: A total of 132 consecutive patients undergoing endoscopic were identified. Of these, 88.0 had brush cytology after ERCP and 44 were Brush cytology with negative pressure. Retrograde cholangiopancreatography (ERCP) including brush cytology and brush cytology with negative pressure in patients with biliary strictures between 2012-2015. Endoscopic retrograde cholangiography was performed with a standard videoduodenoscope Olympus TFJ 160-R (Olympus, Hamburg, Germany) and brush cytology with a Cook medical Double Lumen Biliary BrushTM (Cytology). Means and standard frequencies were used to calculate variables. Results: Positive results for malignancy were obtained in 22 of 88 patients (25%) by brush cytology and 31 of 44 patients (70.4 %) by brush cytology with negative pressure. Conclusions: Sensitivity of cytology sampling could be maximized by negative pressure during ERCP.

Reduction of Components in Cascaded Transformer Multilevel Inverter Using Two DC Sources

Banaei, Mohamad Reza,Salary, Ebrahim,Alizadeh, Ramin,Khounjahan, Hossein The Korean Institute of Electrical Engineers 2012 Journal of Electrical Engineering & Technology Vol.7 No.4

In this paper a novel cascaded transformer multilevel inverter is proposed. Each basic unit of the inverter includes two DC sources, single phase transformers and semiconductor switches. This inverter, which operates as symmetric and asymmetric, can output more number of voltage levels in the same number of the switching devices. Besides, the number of gate driving circuits is reduced, which leads to circuit size reduction and lower power consumption in the driving circuits. Moreover, several methods to determination of transformers turn ratio in proposed inverter are presented. Theoretical analysis, simulation results using MATLAB/SIMULINK and experimental results are provided to verify the operation of the suggested inverter.

Evaluation of Methylobacterium radiotolerance and Sphyngomonas yanoikoaie in Sentinel Lymph Nodes of Breast Cancer Cases

Yazdi, Hamid Reza,Movafagh, Abolfazl,Fallah, Fateme,Shargh, Shohreh Alizadeh,Mansouri, Neda,Pour, Atefeh Heidary,Hashemi, Mehrdad Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup3

It has been established that different kinds of bacteria agents are involved in various cancers. Although the mechanism of tumorigenesis is not clearly understood, there is evidence for the presence of bacteria within tumors, with at least a progression effect for some bacteria that prepare suitable microenvironments for tumor cell growth. The aim of current study was to evaluate bacterial dysbiosis in sentinel lymph nodes of breast cancer patients. One hundred and twenty three fresh-frozen sentinel lymph nodes and a corresponding number of normal adjacent breast tissue specimens and five normal mastectomy samples were investigated employing RT-PCR. In addition using genus-specific primers were applied. There was a significant differences as presence of Methylobacterium radiotolerance DNA recorded between patients and normal control group (p= 0.0). Based on our research work, further studies into the role of microbes in breast cancer would be of great interest.

Selective production of propylene from methanol over nanosheets of metal-substituted MFI zeolites

Naser Hadi,Reza Alizadeh,Aligholi Niaei 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-

The nanosheets of M-substituted (M: Mn, Ce, W) MFI zeolites were successfully synthesized via hydrothermal method. The catalysts were satisfactorily characterized by different techniques and the well synthesized nanostructures were approved. The prepared catalysts were examined in methanol to propylene process and showed improved performance compared to the conventional H-ZSM-5. The Wsubstituted MFI nanosheets productively represented the best performance with complete methanol conversion, propylene selectivity of 55.70%, total selectivity to light olefins of 88.04% and catalytic lifetime of 81 h. Furthermore, TGA and BET analyses were conducted to characterize the coke deposition. The lowest coke was detected on W-substituted MFI nanosheets.

The impact of modified exercise and relaxation therapy on chronic lower back pain in office workers : a randomized clinical trial

Ardalan Shariat,Reza Alizadeh,Vahideh Moradi,Elahe Afsharnia,Azadeh Hakakzadeh,Noureddin Nakhsotin Ansari,Lee Ingle,Brandon S. Shaw,Ina Shaw 한국운동재활학회 2019 JER Vol.15 No.5