A new tyrosinase biosensor based on covalent immobilizationof enzyme on N-(3-aminopropyl) pyrrole polymer film

Rajesh,K.Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polymer film of N-amino substituted pyrrole monomer has been prepared for covalent immobilization of enzyme for biosensing applications, illustrated by tyrosinase (PPO).The tyrosinase enzyme retains its bioactivity when being immobilized on N-substituted pyrrole polymer film by covalent bonding.The enzyme electrode was characterized by UV.Vis and infrared spectroscopy.Phenolic compounds were quantitatively estimated by the direct electrochemical reduction of enzymatically liberated quinone species at 0.2 V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode show sensitivity of 57.6, 71.4 and 45.8 mAM1cm2 and a linear response range of 1.8.170.2, 1.3.110.1 and 2.1.168 lM for phenol, catechol and p-cresol, respectively. The biosensor exhibits a lowest detection limit of 0.9, 0.7 and 1.1 lM, for phenol, catechol and p-cresol, respectively and a period of stable sensitivity of 3 months at 4.5 C.

Simultaneous co-immobilization of enzyme and a redox mediatorin polypyrrole film for the fabrication of an amperometricphenol biosensor

Rajesh,S.S. Pandey,W. Takashima,K. Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polypyrrole lm has been prepared on indium tin oxide coated glass plate by electropolymerization of pyrrole in anaqueous solution of enzyme, tyrosinase (PPO), and a redox mediator potassium hexacyanoferrate (II). The enzyme retains its bio-activity well within the polymer lm. Phenol concentration in aqueous solution was quantitatively estimated by monitoring the elec-tron exchange occurring in redox mediator within the polymer matrix i.e. direct reduction of Fe (III) to Fe (II) ion at +0.12V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode (PPO/Fe2+/PPY/ITO) show a sensitivityof 0.33 AM. 1 cm. 2 and a linear response range of 4.5107.4l M for phenol in aqueous medium. The electrode exhibits a responsetime of about 80 second and is stable for 4 months at 4.C..

A novel hybrid technique to treat flush mesenteric arterial occlusion in acute-on-chronic mesenteric ischemia

Rajesh Girdhardas Mundhada,Atul Dharmaraj Rewatkar,Aishwarya Atul Rewatkar,Anju Rajesh Mundhada,Nikita Navin Chandak 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

Acute mesenteric occlusion is a life-threatening condition with a significant mortality rate and requires prompt diagnosis and revascularization using open repair, a hybrid procedure, or endovascular intervention. The open approach is associated with significant morbidity and mortality, while endovascular procedures are done by the antegrade or retrograde open mesenteric stent technique and have been observed to improve the prognosis and outcomes of mesenteric ischemia. We report three cases of acute-on-chronic mesenteric ischemia secondary to occlusion with failed percutaneous mesenteric artery stenting. They were successfully treated using a novel hybrid technique, without the need for mesenteric arteriotomy or subsequent arterial repair.

Effect of Preoperative Vitamin D Levels on Functional Performance after Total Knee Arthroplasty

Rajesh Navin Maniar,Aniket Machindra Patil,Adit Rajesh Maniar,Bharat Gangaraju,Jaivardhan Singh 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.2

Background: Low vitamin D levels affects muscle function. Vitamin D and calcium deficiency cause osteomalacic myopathy and poor functional recovery after hip fractures. The relationship of vitamin D and functional performance after total knee arthroplasty (TKA) is not previously reported. Methods: Influence of vitamin D on functional performance before and after TKA was reviewed retrospectively in 120 patients. Of these, 64 had vitamin D deficiency (25-hydroxy vitamin D < 30 ng/mL) preoperatively. All 120 patients received vitamin D oral supplementation postoperatively. Functional parameters including Western Ontario and McMaster Osteoarthritis Index (WOMAC), Short-Form Health Survey questionnaire, and Knee Society Score were assessed pre- and postoperatively at 3 months. Results: Preoperative function was significantly lower in osteoarthritic patients with vitamin D deficiency (WOMAC score; p = 0.040), but at 3 months all functional scores were similar. Conclusions: We concluded that vitamin D deficiency has a negative effect on function in knee osteoarthritic patients. However, postoperative vitamin D supplementation can lead to functional recovery in these patients. Thus, TKA should not be delayed in vitamin D deficient patients; rather supplementation in the postoperative period is preferable to achieve comparable functional outcome at 3 months to patients with vitamin D sufficiency.

Ratio-Cum-Product Estimators of Population Mean Using Known Population Parameters of Auxiliary Variates

Tailor, Rajesh,Parmar, Rajesh,Kim, Jong-Min,Tailor, Ritesh The Korean Statistical Society 2011 Communications for statistical applications and me Vol.18 No.2

This paper suggests two ratio-cum-product estimators of finite population mean using known coefficient of variation and co-efficient of kurtosis of auxiliary characters. The bias and mean squared error of the proposed estimators with large sample approximation are derived. It has been shown that the estimators suggested by Upadhyaya and Singh (1999) are particular case of the suggested estimators. Almost ratio-cum product estimators of suggested estimators have also been obtained using Jackknife technique given by Quenouille (1956). An empirical study is also carried out to demonstrate the performance of the suggested estimators.

Evaluation of Machine Learning Algorithms for Detection of Malicious Traffic in SCADA Network

Rajesh L.,Satyanarayana Penke 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.2

Industrial Process Control Systems (IPCS) like Supervisory Control and Data Acquisition (SCADA) systems are more vulnerable to cyber-attacks. Detection of malicious traffi c in IPCS-SCADA network using machine learning techniques is one of the security enhancement methods of Industrial Process Control Systems. The existing network intrusion detection methods used limited old data sets and little addressed for IPCS-SCADA specifi c network. In this paper we evaluated machine learning algorithms with and with out fi ltering and sampling techniques for intrusion detection in IPCS-SCADA network. In this work, we generated own data set with network traffi c contains both normal and attack data using a real time SCADA test bed. Next we applied feature extraction techniques Chi-Square, ANOVA and Least Absolute Shrinkage and Selection Operator (LASSO) to reduce the feature set dimensionality. We applied SVM variant Synthetic Minority Oversampling Technique (SVMSMOTE) for handling this imbalance data set. After that we used four Machine Learning (ML) algorithms like Random Forest (RF), Support Vector Machine (SVM), K-nearest neighbors (KNN) and Naive Bayes (NB) and calculated various performance metrics like Accuracy, Matthews correlation coeffi cient (MCC), recall, F1-score, FNR and ROC. We evaluated the performance of these ML algorithms with (a) ML techniques only (b) ML techniques with feature selection methods (c) ML techniques with SVMSMOTE only (d) ML techniques with both feature selection methods and SVMSMOTE. We concluded from the results that SVM algorithm with fi ltering and SVMSMOTE technique outperforms compared to other three machine learning algorithms, with ROC value of 99.96%.

Inter-oraganisational Information Systems (IOIS) and Cybermediaries for Agriculture and Food Supply Chain

Rajesh Rajaguru,Bobby Swar 한국농식품정보과학회 2013 Agribusiness and Information Management Vol.5 No.1

Competition in supply chain has moved from between being organizations to between supply chain. To enhance competitiveness in supply chain, there is a need for organisations to establish and manage collaborative relationships among supply chain partners and share operational and strategic information timely and accurately. As a result, food supply chain partners have adopted of inter organisational information systems (IOIS) such as EDI, IEDI, RFID, internet, and extranets to facilitate exchange of information, streamline production and to effectively manage inventory processes so as to deliver value to the customer. Together with IOIS, cybermediaries also plays a key role in facilitating agriculture and food supply chain. This paper investigates the role and significance of IOIS and cybermediaries in facilitating agriculture and food supply chain. Convergent interviewing was conducted with agriculture producers, food processors and retailers. The study identifi ed logistics, supply chain and customer services benefi ts perceived from implementing IOIS and utilising cybermediaries services in the food supply chain. To conclude the conceptual paper outlined managerial implications and areas for future research.

Determinants of COVID-19 Vaccination Willingness among Health Care Workers: A Quick Online Survey in India

Rajesh Kumar,Kalpana Beniwal,Yogesh Bahurupi,Ravi Kant,Mukesh Bairwa 대한가정의학회 2021 Korean Journal of Family Medicine Vol.42 No.6

Background: The coronavirus disease 2019 (COVID-19) pandemic has caused a large number of deaths along with severe socio-economic effects. The vaccine is considered to be the last hope to control viral transmission. This study aimed to explore the determinants of health care workers’ (HCWs) willingness to take the COVID-19 vaccina-tion.Methods: A structured, pre-validated, and pre-tested questionnaire was administered online to 599 HCWs includ-ing physicians, residents, and nurses from different types of healthcare set-ups across India. Information was col-lected regarding vaccine acceptability, attitude toward vaccination, and reasons for hesitancy. The chi-square test, followed by multinomial regression analysis, was applied to determine the factors associated with HCWs’ vaccina-tion willingness.Results: It was found that 73 % (n=437) of HCWs were willing to accept the vaccines, while 10.85% (n=65) refused and 16.2% (n=96) needed more time to decide. Gender (P<0.001), occupation (P=0.040), working as front-line workers (P=0.008), vaccine manufacturing country preferences (P<0.001), and perceived risk of catching COVID-19 in the next 6 months (P=0.005) had a significant association with intent to receive vaccination (the response were “yes” vs. “no” and “not sure”). The reasons for vaccine hesitancy were vaccine safety and efficacy concerns, antivac-cine attitude and beliefs, personal choice, and not wanting to take a vaccine before others.Conclusion: The majority of HCWs agreed to take COVID-19 vaccines once available. Nevertheless, providing sup-port to manage evolving vaccine environments will help change the perception of HCWs who refuse or are reluc-tant to take the vaccines.

2010 Asian WTO Research Network (AWRN) Seoul Meeting : Dispute Resolution Mechanism in the Trade Agreements of India -An example from India-Chile PTA

( Rajesh Sharma ) 고려대학교 법학연구원 2010 Korea University Law Review Vol.8 No.-

Inactivation of phosphomannose isomerase gene abolishes sporulation and antibiotic production in Streptomyces coelicolor.

Rajesh, Thangamani,Song, Eunjung,Kim, Ji-Nu,Lee, Bo-Rahm,Kim, Eun-Jung,Park, Sung-Hee,Kim, Yun-Gon,Yoo, Dongwon,Park, Hyung-Yeon,Choi, Yun-Hui,Kim, Byung-Gee,Yang, Yung-Hun Springer International 2012 Applied microbiology and biotechnology Vol.93 No.4

<P>Phosphomannose isomerases (PMIs) in bacteria and fungi catalyze the reversible conversion of d-fructose-6-phosphate to d-mannose-6-phosphate during biosynthesis of GDP-mannose, which is the main intermediate in the mannosylation of important cell wall components, glycoproteins, and certain glycolipids. In the present study, the kinetic parameters of PMI from Streptomyces coelicolor were obtained, and its function on antibiotic production and sporulation was studied. manA (SCO3025) encoding PMI in S. coelicolor was deleted by insertional inactivation. Its mutant (S. coelicolora dagger manA) was found to exhibit a bld-like phenotype. Additionally, S. coelicolora dagger manA failed to produce the antibiotics actinorhodin and red tripyrolle undecylprodigiosin in liquid media. To identify the function of manA, the gene was cloned and expressed in Escherichia coli BL21 (DE3). The purified recombinant ManA exhibited PMI activity (K (cat)/K (m) (mM(-1) s(-1) = 0.41 for d-mannose-6-phosphate), but failed to show GDP-d-mannose pyrophosphorylase [GMP (ManC)] activity. Complementation analysis with manA from S. coelicolor or E. coli resulted in the recovery of bld-like phenotype of S. coelicolora dagger manA. SCO3026, another ORF that encodes a protein with sequence similarity towards bifunctional PMI and GMP, was also tested for its ability to function as an alternate ManA. However, the purified protein of SCO3026 failed to exhibit both PMI and GMP activity. The present study shows that enzymes involved in carbohydrate metabolism could control cellular differentiation as well as the production of secondary metabolites.</P>